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    • 5. 发明授权
    • tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same
    • tdcBC / pckA基因灭活的微生物和使用其生产L-苏氨酸的方法
    • US07485450B2
    • 2009-02-03
    • US10817044
    • 2004-04-02
    • Young Hoon ParkByoung Choon LeeDae Cheol KimJin Ho LeeJae Yong Cho
    • Young Hoon ParkByoung Choon LeeDae Cheol KimJin Ho LeeJae Yong Cho
    • C12N1/20
    • C12P13/08C12N9/88
    • The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
    • 本发明提供了包含灭活的染色体tdcBC基因和灭活的染色体pckA基因的微生物,其显着提高了L-苏氨酸的生产率。 另外,本发明提供使用该微生物生产L-苏氨酸的方法。 通过重组技术将抗生素抗性基因并入包含失活的L-苏氨酸降解相关操纵子基因tdcBC的细菌菌株的染色体上的pckA基因中来制备微生物。 该微生物具有防止由于tdcBC操纵子基因失活引起的L-苏氨酸的降解和细胞内流入的作用,并且包括用于L-苏氨酸生物合成的更多活化途径。 因此,即使在高浓度葡萄糖的存在下,微生物也能够以高水平和高产率生产L-苏氨酸,因此可用于批量生产L-苏氨酸。