专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US4373026A Microbial polyamide oxidase PC-3 and process for preparation thereof 失效
标题翻译：微生物聚酰胺氧化酶PC-3及其制备方法
公开(公告)号：US4373026A
公开(公告)日：1983-02-08
申请号：US216302
申请日：1980-12-12
申请人： Hideaki Yamada , Yoshiki Tani , Kimiyasu Isobe
发明人： Hideaki Yamada , Yoshiki Tani , Kimiyasu Isobe
IPC分类号： C12N9/06 , C12Q1/26 , C12R1/82 , C12P21/00
CPC分类号： C12N9/0026 , C12Q1/26 , Y10S435/935
摘要： A novel polyamine oxidase, polyamine oxidase PC-3, takes an important role participating in decomposition and metabolism and polyamines such as spermidine and spermine.
摘要翻译：一种新型多胺氧化酶多胺氧化酶PC-3在分解代谢和多胺如亚精胺和精胺中起重要作用。

2. 发明授权

US4371621A Microbial polyamine oxidase AT-1 失效
标题翻译：微生物多胺氧化酶AT-1
公开(公告)号：US4371621A
公开(公告)日：1983-02-01
申请号：US216301
申请日：1980-12-12
申请人： Hideaki Yamada , Yoshiki Tani , Kimiyasu Isobe
发明人： Hideaki Yamada , Yoshiki Tani , Kimiyasu Isobe
IPC分类号： C12N9/06 , C12R1/66 , C12R1/82
CPC分类号： C12N9/0026 , Y10S435/913
摘要： A novel polyamine oxidase, polyamine oxidase AT-1, takes an important role participating in decomposition and metabolism of polyamines such as spermidine and spermine.
摘要翻译：一种新型多胺氧化酶多胺氧化酶AT-1在多胺如亚精胺和精胺的分解和代谢中起重要作用。

3. 发明授权

US4617263A Method for quantitative determination of acetylputrescine in a mixture 失效
标题翻译：用于定量测定混合物中乙酰肌氨酸的方法
公开(公告)号：US4617263A
公开(公告)日：1986-10-14
申请号：US776162
申请日：1985-09-13
申请人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
发明人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
IPC分类号： G01N33/52 , C12Q1/26 , G01N33/50
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other diseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译：提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的个体游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰精氨酸和乙酰胞嘧啶诊断和治疗。该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应，优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量步骤和求解联立方程。

4. 发明授权

US4550078A Method for the quantitative determination of individual polyamine components in a mixture 失效
标题翻译：用于定量测定混合物中单个多胺组分的方法
公开(公告)号：US4550078A
公开(公告)日：1985-10-29
申请号：US495677
申请日：1983-05-18
申请人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
发明人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
IPC分类号： G01N33/52 , C12Q1/26 , G01N33/50
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other deseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译：提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的各种游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰哌啶和乙酰吗啡诊断和治疗。该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应，优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量步骤和求解联立方程。

5. 发明授权

US4999289A Lipase, its production and use for assay of triglycerides 失效
标题翻译：脂肪酶，其生产和用于测定甘油三酯
公开(公告)号：US4999289A
公开(公告)日：1991-03-12
申请号：US34452
申请日：1987-04-06
申请人： Tetsunori Akiba , Shotaro Yamaguchi , Satoru Suzuki , Kimiyasu Isobe , Kuniyoshi Matsunaga
发明人： Tetsunori Akiba , Shotaro Yamaguchi , Satoru Suzuki , Kimiyasu Isobe , Kuniyoshi Matsunaga
IPC分类号： C12N9/20 , C12Q1/44
CPC分类号： C12N9/20 , C12Q1/44 , Y10S435/933
摘要： Lipase isolated from Penicillium characterized by the following properties:(1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms;(2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid;(3) Optimal pH range for said hydrolysis 5-7;(4) Stable in the pH range of 4.5-6;(5) Optimal temperature range for activity 35.degree.-40.degree. C.;(6) thermal stability up to about 35.degree. C.;(7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%;(8) Molecular weight about 1000,000 to 120,000;(9) Isoelectric point about pH 3.84.
摘要翻译：从青霉菌分离的脂肪酶，其特征在于以下性质：（1）有效地水解具有4-18个碳原子的脂肪酸的甘油三酯; （2）用这种脂肪酶水解这种甘油三酸酯每100摩尔脂肪酸产生至少5摩尔甘油; （3）所述水解5-7的最佳pH范围; （4）在4.5-6的pH范围内稳定; （5）最适温度范围为35°-40°C。（6）热稳定性高达约35℃。（7）表面活性剂活化，但浓度高达5％时，这些表面活性剂基本上不被抑制; （8）分子量约为1000,000至120,000; （9）等电点约pH3.84。

6. 发明申请

US20120149071A1 D-AMINOACYLASE 审中-公开
标题翻译： D-氨基酸
公开(公告)号：US20120149071A1
公开(公告)日：2012-06-14
申请号：US13345399
申请日：2012-01-06
申请人： Kimiyasu Isobe , Seiki Yamaguchi
发明人： Kimiyasu Isobe , Seiki Yamaguchi
IPC分类号： C12N9/80 , C12N1/20 , C12P13/04 , C12N1/21 , C12N15/55 , C12N15/74
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost.A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译：提供了具有高底物特异性的D-氨基酸酰化酶。该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。 Mn2 +，Co2 +，Ni2 +和Zn2 +各自以1 mmol / L的速度抑制其活性，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各自以5 mmol / L的速度进行抑制。

7. 发明授权

US06943004B1 Transformed microorganism and process for producing D-aminoacylase 失效
标题翻译：转化微生物和生产D-氨基酰化酶的方法
公开(公告)号：US06943004B1
公开(公告)日：2005-09-13
申请号：US10009782
申请日：2000-06-15
申请人： Ken-ichi Takeuchi , Yoshinao Koide , Yoshihiko Hirose , Mitsuaki Moriguchi , Kimiyasu Isobe
发明人： Ken-ichi Takeuchi , Yoshinao Koide , Yoshihiko Hirose , Mitsuaki Moriguchi , Kimiyasu Isobe
IPC分类号： C12N15/09 , C12N1/21 , C12N9/80 , C12N9/78 , C07H21/02 , C12N1/20 , C12N15/00 , C12P21/06
CPC分类号： C12N9/80
摘要： A transformed microorganism prepared by inserting into a host microorganism with zinc tolerance a D-aminoacylase-producing gene which selectively produces D-aminoacylase alone between D-aminoacylase and L-aminoacylase. A process comprising culturing the transformed microorganism in a culture medium containing zinc ion and obtaining D-aminoacylase from the culture at a high efficiency.
摘要翻译：通过将具有耐受性的D-氨基酸酰化酶产生基因插入具有耐受性的宿主微生物中的转化的微生物，其在D-氨基酸酰化酶和L-氨基酸酰化酶之间单独选择性地产生D-氨基酸酰化酶。一种方法，包括在含有锌离子的培养基中培养转化的微生物，并从培养物中以高效率获得D-氨基酰化酶。

8. 发明申请

US20080096244A1 D-AMINOACYLASE 审中-公开
标题翻译： D-氨基酸
公开(公告)号：US20080096244A1
公开(公告)日：2008-04-24
申请号：US11627256
申请日：2007-01-25
申请人： Kimiyasu Isobe , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
发明人： Kimiyasu Isobe , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
IPC分类号： C12P21/04 , C12N1/20 , C12N15/00 , C12N15/11 , C12N9/00
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译：提供了具有高底物特异性的D-氨基酸酰化酶。该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。并且其活性受到Mn 2+，Co 2+，Ni 2+，Zn 2+，Zn 2+，每个为1mmol / L，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各为5mmol / L。

9. 发明申请

US20060172375A1 D-aminoacylase 审中-公开
标题翻译： D-氨基酰化酶
公开(公告)号：US20060172375A1
公开(公告)日：2006-08-03
申请号：US10539281
申请日：2003-12-17
申请人： Kimiyasu Isobe , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
发明人： Kimiyasu Isobe , Seiki Yamaguchi , Masayuki Kobayashi , Shinya Kumagai , Takami Sarashina
IPC分类号： C12P13/04 , C07H21/04 , C12P21/06 , C12N9/80 , C12N1/21 , C12N15/74
CPC分类号： C12N9/80
摘要： A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.
摘要翻译：提供了具有高底物特异性的D-氨基酸酰化酶。该D-氨基酸酶可以以低成本方便且有效地从N-乙酰基-D，L-氨基酸产生D-氨基酸。由去纤维杆菌属的微生物产生的D-氨基酸酰化酶; 其作用于N-乙酰基-D-氨基酸; 其具有约55,000道尔顿的分子量（通过电泳测定）和5.3的等电点（通过用于变性体系的二维电泳测定）; 其作用于N-乙酰基-D-缬氨酸，N-乙酰基-D-亮氨酸等，但不适用于N-乙酰基-L-缬氨酸，N-乙酰基-L-亮氨酸等; 其最适温度为37℃（pH8），最适pH值为8〜8.5，在37℃下。并且其活性受到Mn 2+，Co 2+，Ni 2+，Zn 2+，Zn 2+，每个为1mmol / L，二硫苏糖醇，2-巯基乙醇，邻菲咯啉和L-半胱氨酸各为5mmol / L。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式