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    • 2. 发明授权
    • Method for the determination of cholesterol
    • 胆固醇测定方法
    • US4892816A
    • 1990-01-09
    • US136522
    • 1987-12-22
    • Tetsunori AkibaKuniyoshi Matsunaga
    • Tetsunori AkibaKuniyoshi Matsunaga
    • C12Q1/32C12Q1/44C12Q1/60
    • C12Q1/60Y10S435/81
    • An enzymatic method for measurement of cholesterol comprises incubating:(a) a test sample;(b) a cholesterol dehydrogenase;(c) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(d) a surfactantand measuring the resulting detectable oxidized and reduced products kinetically.A composition for the kinetic measurement of cholesterol comprises:(a) a cholesterol dehydrogenase;(b) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(c) a surfactant.
    • 用于测量胆固醇的酶法包括孵育:(a)测试样品; (b)胆固醇脱氢酶; (c)选自烟酰胺 - 腺嘌呤二核苷酸(NAD)和烟酰胺 - 腺嘌呤二核苷酸磷酸(NADP)的氧化剂; 和(d)表面活性剂并动态地测量所得可检测的氧化和还原产物。 用于胆固醇动力学测定的组合物包括:(a)胆固醇脱氢酶; (b)选自烟酰胺 - 腺嘌呤二核苷酸(NAD)和烟酰胺 - 腺嘌呤二核苷酸磷酸(NADP)的氧化剂; 和(c)表面活性剂。