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1. 发明授权

US4617263A Method for quantitative determination of acetylputrescine in a mixture 失效
标题翻译：用于定量测定混合物中乙酰肌氨酸的方法
公开(公告)号：US4617263A
公开(公告)日：1986-10-14
申请号：US776162
申请日：1985-09-13
申请人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
发明人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
IPC分类号： G01N33/52 , C12Q1/26 , G01N33/50
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other diseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译：提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的个体游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰精氨酸和乙酰胞嘧啶诊断和治疗。该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应，优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量步骤和求解联立方程。

2. 发明授权

US4550078A Method for the quantitative determination of individual polyamine components in a mixture 失效
标题翻译：用于定量测定混合物中单个多胺组分的方法
公开(公告)号：US4550078A
公开(公告)日：1985-10-29
申请号：US495677
申请日：1983-05-18
申请人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
发明人： Hideaki Yamada , Shogo Otsuji , Kimiyasu Isobe , Kuniyoshi Matsunaga , Tadahiko Inukai
IPC分类号： G01N33/52 , C12Q1/26 , G01N33/50
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other deseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译：提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的各种游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰哌啶和乙酰吗啡诊断和治疗。该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应，优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量步骤和求解联立方程。

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