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    • 3. 发明授权
    • Method for preparing pure lactic acid
    • 纯乳酸的制备方法
    • US5932455A
    • 1999-08-03
    • US945874
    • 1997-11-07
    • Tapio ViljavaHannu Koivikko
    • Tapio ViljavaHannu Koivikko
    • C12P7/56C12R1/01C12R1/225
    • C12P7/56Y10S435/822Y10S435/853Y10S435/885
    • The invention relates to a method for preparing pure lactic acid or a salt thereof by fermentation. The preparation process comprises bioreactor refreshing cycle and a lactic acid production cycle, wherein during the production cycle a solution comprising substantially pure feedstock is recycled through a bioreactor containing refreshed microorganism cells, the lactic acid produced being neutralized by adding an alkali, and the recycling is discontinued when the alkali consumption is substantially diminished, and during the refreshing cycle the microorganism cells are refreshed by recycling through the bioreactor a carbohydrate solution enriched with nutrients, thus replenishing the capacity of the microorganisms to produce an acid, and recovery of lactate or conversion thereof into lactic acid or other salt.
    • PCT No.PCT / FI96 / 00415 Sec。 371日期:1997年11月7日 102(e)1997年11月7日PCT PCT 1996年7月16日PCT公布。 出版物WO97 / 04120 PCT 日期:1997年2月6日本发明涉及通过发酵制备纯乳酸或其盐的方法。 制备方法包括生物反应器更新循环和乳酸生产循环,其中在生产循环期间,包含基本上纯的原料的溶液通过含有更新的微生物细胞的生物反应器再循环,所产生的乳酸通过加入碱中和,回收利用 当碱消耗量基本上减少时停止,并且在清新循环期间,微生物细胞通过生物反应器再循环富含营养物的碳水化合物溶液而被刷新,从而补充微生物产生酸的能力,以及乳酸的回收或转化 变成乳酸或其他盐。
    • 4. 发明授权
    • Immobilization of microogranisms on weakly basic anion exchange
substance for producing isomaltulose
    • 将微生物固定在用于转化蔗糖的阴离子交换物质上
    • US5939294A
    • 1999-08-17
    • US857808
    • 1997-05-16
    • Marja-Leena SarkkiHeikki HeikkilaTapio Viljava
    • Marja-Leena SarkkiHeikki HeikkilaTapio Viljava
    • C12N9/90C12N11/12C12P19/24C12P19/12C12N11/02C12N11/08
    • C12R1/01C12N11/12C12N9/90C12P19/24
    • Isomaltulose-forming microorganisms are immobilized on a carrier that is a weakly basic anion exchange substance in the form of a substantially non-compressible porous particulate solid material, and are used for isomerization of sucrose to isomaltulose. A preferred carrier contains microfibers or microparticles of diethylaminoethyl cellulose adherently bound by agglomeration with polystyrene. The isomerization may be a continuous conversion in one or more columns packed with the carrier. Isomaltulose may be hydrogenated to form isomalt for use in sweetening. Microorganisms can be immobilized on the carrier by feeding microorganisms to a column containing the carrier. After microorganism immobilization, the carrier may be treated with a crosslinking and/or flocculating compound. Regeneration of the carrier is carried out by removing microorganisms, washing and reloading with fresh microorganisms.
    • 异麦芽酮糖形成微生物固定在作为基本上不可压缩的多孔颗粒固体材料形式的弱碱性阴离子交换物质的载体上,并用于将蔗糖异构化成异麦芽酮糖。 优选的载体包含通过与聚苯乙烯附聚粘附结合的二乙基氨基乙基纤维素的微纤维或微粒。 异构化可以是在一个或多个填充有载体的柱中的连续转化。 异麦芽酮糖可以被氢化以形成用于增甜的异麦芽酮糖醇。 微生物可以通过将微生物送入含有载体的柱中而固定在载体上。 微生物固定后,载体可以用交联和/或絮凝化合物处理。 通过除去微生物,用新鲜的微生物清洗和重新装载来进行载体的再生。