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    • 8. 发明授权
    • Immobilization of microogranisms on weakly basic anion exchange
substance for producing isomaltulose
    • 将微生物固定在用于转化蔗糖的阴离子交换物质上
    • US5939294A
    • 1999-08-17
    • US857808
    • 1997-05-16
    • Marja-Leena SarkkiHeikki HeikkilaTapio Viljava
    • Marja-Leena SarkkiHeikki HeikkilaTapio Viljava
    • C12N9/90C12N11/12C12P19/24C12P19/12C12N11/02C12N11/08
    • C12R1/01C12N11/12C12N9/90C12P19/24
    • Isomaltulose-forming microorganisms are immobilized on a carrier that is a weakly basic anion exchange substance in the form of a substantially non-compressible porous particulate solid material, and are used for isomerization of sucrose to isomaltulose. A preferred carrier contains microfibers or microparticles of diethylaminoethyl cellulose adherently bound by agglomeration with polystyrene. The isomerization may be a continuous conversion in one or more columns packed with the carrier. Isomaltulose may be hydrogenated to form isomalt for use in sweetening. Microorganisms can be immobilized on the carrier by feeding microorganisms to a column containing the carrier. After microorganism immobilization, the carrier may be treated with a crosslinking and/or flocculating compound. Regeneration of the carrier is carried out by removing microorganisms, washing and reloading with fresh microorganisms.
    • 异麦芽酮糖形成微生物固定在作为基本上不可压缩的多孔颗粒固体材料形式的弱碱性阴离子交换物质的载体上,并用于将蔗糖异构化成异麦芽酮糖。 优选的载体包含通过与聚苯乙烯附聚粘附结合的二乙基氨基乙基纤维素的微纤维或微粒。 异构化可以是在一个或多个填充有载体的柱中的连续转化。 异麦芽酮糖可以被氢化以形成用于增甜的异麦芽酮糖醇。 微生物可以通过将微生物送入含有载体的柱中而固定在载体上。 微生物固定后,载体可以用交联和/或絮凝化合物处理。 通过除去微生物,用新鲜的微生物清洗和重新装载来进行载体的再生。
    • 9. 发明授权
    • Method for the production of xylitol
    • 生产木糖醇的方法
    • US07482144B2
    • 2009-01-27
    • US10341220
    • 2003-01-13
    • Heikki OjamoMerja PenttilaHeikki HeikkilaJaana UusitaloMarja IlmenMarja-Leena SarkkiMaija-Leena Vehkomaki
    • Heikki OjamoMerja PenttilaHeikki HeikkilaJaana UusitaloMarja IlmenMarja-Leena SarkkiMaija-Leena Vehkomaki
    • C12P7/18C12N7/16C12N1/18
    • C12P7/18
    • The invention relates to a method for the production of xylitol, the method comprising (a1) providing (i) a microorganism having xylanolytic activity, and (ii) a microorganism capable of converting a pentose sugar to xylitol; or (a2) providing a microorganism having xylanolytic activity and being capable of converting a pentose sugar to xylitol, (b) culturing the microorganism of step (a1) (i) or the microorganism of step (a2) in a medium comprising polymer or oligomer materials containing pentose sugars in conditions sufficient for enabling hydrolysis of said polymers or oligomers by the microorganism; (c) producing xylitol in the microorganism of step (a1) (ii) or in the microorganism of step (a2) by bioconversion of the hydrolysis products obtained in step (b), and (d) recovering said xylitol produced. The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.
    • 本发明涉及一种生产木糖醇的方法,所述方法包括(a1)提供(i)具有木聚糖分解活性的微生物和(ii)能够将戊糖转化成木糖醇的微生物; 或(a2)提供具有木聚糖分解活性并能够将戊糖转化为木糖醇的微生物,(b)在包含聚合物或低聚物的培养基中培养步骤(a1)(i)的微生物或步骤(a2)的微生物) 在足以使微生物水解所述聚合物或低聚物的条件下含有戊糖的材料; (c)通过步骤(b)中获得的水解产物的生物转化和(d)回收所生产的木糖醇,在步骤(a1)(ⅱ)的微生物中或在步骤(a2)的微生物中生产木糖醇。 本发明还涉及一种具有木聚糖分解活性且经遗传修饰的微生物(i)增强其木聚糖分解活性,和(ii)降低其木糖醇代谢。