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1. 发明申请

US20130203617A1 Glycoprotein Analysis Kit and Use Thereof 审中-公开
标题翻译：糖蛋白分析试剂盒及其用途
公开(公告)号：US20130203617A1
公开(公告)日：2013-08-08
申请号：US13695197
申请日：2011-04-29
申请人： Hak Sung Kim , Sun Young Park , Sang Chul Lee , Eun Kyoung Kim , Sung Ho Kim , Wan Jung Kim , Yong Tae Kim , Young Dok Son , Jung Hoe Kim
发明人： Hak Sung Kim , Sun Young Park , Sang Chul Lee , Eun Kyoung Kim , Sung Ho Kim , Wan Jung Kim , Yong Tae Kim , Young Dok Son , Jung Hoe Kim
IPC分类号： G01N33/74 , C12N15/85 , G01N33/68
CPC分类号： G01N33/746 , C12N15/85 , G01N33/6803 , G01N33/6842 , G01N2400/02
摘要： Disclosed are a glycoprotein analysis kit and use thereof. The glycoprotein analysis kit comprises a fluorescently labeled antibody, a fluorescently labeled biomaterial, and a support, and is used in a dual probing method for analyzing the content of a glycoprotein and profiling the oligosaccharide chain, simultaneously, and a method for selecting a single cell producing the glycoprotein having a desired glycosylation pattern. Also, a single cell producing the glycoprotein having a desired glycosylation pattern is provided.
摘要翻译：公开了糖蛋白分析试剂盒及其用途。糖蛋白分析试剂盒包含荧光标记的抗体，荧光标记的生物材料和载体，并用于同时用于分析糖蛋白含量和分析寡糖链的双重探测方法，以及选择单细胞的方法产生具有所需糖基化模式的糖蛋白。此外，提供了产生具有所需糖基化模式的糖蛋白的单细胞。

2. 发明授权

US08940876B2 Method for preparing recombinant glycoproteins with high sialic acid content 有权
标题翻译：制备高唾液酸含量的重组糖蛋白的方法
公开(公告)号：US08940876B2
公开(公告)日：2015-01-27
申请号：US13390671
申请日：2011-02-01
申请人： Jung Hoe Kim , Young Dok Son , Jin Young Hwang , Yeon Tae Jeong
发明人： Jung Hoe Kim , Young Dok Son , Jin Young Hwang , Yeon Tae Jeong
IPC分类号： C07K14/505 , C07K14/705 , C07K14/52 , C12P21/00 , C12N9/90 , C12N9/12 , C12N9/10
CPC分类号： C07K14/505 , C07K14/524 , C07K14/705 , C12N9/1081 , C12N9/1205 , C12N9/90 , C12P21/005 , C12Y501/03014
摘要： The present disclosure relates to a method for preparing recombinant glycoproteins with high sialic acid content. More specifically, for UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE/MNK) enzyme where point mutation was induced by substituting arginine at position 263 by leucine only or by further substituting arginine at position 266 by glutamine, epimerase activity is constantly maintained, and overexpressed cells thereof experience an increase in intracellular cytidine monophosphate (CMP)-sialic acid content, irrespective of CMP-sialic acid concentration. Particularly, since in an glycoprotein (such as, erythropoietin and thrombopoietin)-producing host cell where point mutationinduced GNE/MNK, human alpha-2,3-sialyltransferase and a CMP-sialic acid transporter gene are simultaneously overexpressed, intracellular content of CMP-sialic acid and sialic acid in glycoprotein increases in cells, overexpression in a host cell producing a sialylated recombinant glycoprotein the three genes above may be useful for preparing glycoprotein with increased sialic acid content.
摘要翻译：本公开涉及一种制备高唾液酸含量的重组糖蛋白的方法。更具体地，对于通过仅通过亮氨酸取代位置263处的精氨酸或通过用谷氨酰胺进一步取代位置266处的精氨酸而诱发点突变的UDP-GlcNAc 2-差向异构酶/ ManNAc激酶（GNE / MNK）酶，不断维持差向异构酶活性，并且其过表达的细胞经历细胞内胞苷酸单磷酸（CMP） - 唾液酸含量的增加，而不考虑CMP-唾液酸浓度。特别是，由于在产生点突变诱导的GNE / MNK的糖蛋白（例如促红细胞生成素和血小板生成素）中，人α-2,3-唾液酸转移酶和CMP-唾液酸转运蛋白基因同时过表达，因此CMP- 糖蛋白中的唾液酸和唾液酸在细胞中增加，在产生唾液酸化重组糖蛋白的宿主细胞中过表达，上述三种基因可能用于制备具有增加的唾液酸含量的糖蛋白。

3. 发明申请

US20120149874A1 METHOD FOR PREPARING RECOMBINANT GLYCOPROTEINS WITH HIGH SIALIC ACID CONTENT 有权
标题翻译：用高碱性内容物制备重组蛋白糖的方法
公开(公告)号：US20120149874A1
公开(公告)日：2012-06-14
申请号：US13390671
申请日：2011-02-01
申请人： Jung Hoe Kim , Young Dok Son , Jin Young Hwang , Yeon Tae Jeong
发明人： Jung Hoe Kim , Young Dok Son , Jin Young Hwang , Yeon Tae Jeong
IPC分类号： C07K14/435 , C12N9/18 , C12N9/10 , C07K14/575 , C12N15/81 , C07K14/745 , C12N15/85 , C12N1/19 , C12N5/10 , C12P21/00 , C07K14/755
CPC分类号： C07K14/505 , C07K14/524 , C07K14/705 , C12N9/1081 , C12N9/1205 , C12N9/90 , C12P21/005 , C12Y501/03014
摘要： The present disclosure relates to a method for preparing recombinant glycoproteins with high sialic acid content. More specifically, for UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE/MNK) enzyme where point mutation was induced by substituting arginine at position 263 by leucine only or by further substituting arginine at position 266 by glutamine, epimerase activity is constantly maintained, and overexpressed cells thereof experience an increase in intracellular cytidine monophosphate (CMP)-sialic acid content, irrespective of CMP-sialic acid concentration. Particularly, since in an glycoprotein(such as, erythropoietin and thrombopoietin)-producing host cell where point mutationinduced GNE/MNK, human alpha-2,3-sialyltransferase and a CMP-sialic acid transporter gene are simultaneously overexpressed, intracellular content of CMP-sialic acid and sialic acid in glycoprotein increases in cells, overexpression in a host cell producing a sialylated recombinant glycoprotein the three genes above may be useful for preparing glycoprotein with increased sialic acid content.
摘要翻译：本公开涉及一种制备高唾液酸含量的重组糖蛋白的方法。更具体地，对于通过仅通过亮氨酸取代位置263处的精氨酸或通过用谷氨酰胺进一步取代位置266处的精氨酸而诱发点突变的UDP-GlcNAc 2-差向异构酶/ ManNAc激酶（GNE / MNK）酶，不断维持差向异构酶活性，并且其过表达的细胞经历细胞内胞苷酸单磷酸（CMP） - 唾液酸含量的增加，而不考虑CMP-唾液酸浓度。特别是，由于在产生点突变诱导的GNE / MNK的糖蛋白（例如促红细胞生成素和血小板生成素）中，人α-2,3-唾液酸转移酶和CMP-唾液酸转运蛋白基因同时过表达，因此CMP- 糖蛋白中的唾液酸和唾液酸在细胞中增加，在产生唾液酸化重组糖蛋白的宿主细胞中过表达，上述三种基因可能用于制备具有增加的唾液酸含量的糖蛋白。

4. 发明授权

US08959026B2 Apparatus and method for encoding/decoding multichannel signal 有权
标题翻译：用于对多声道信号进行编码/解码的装置和方法
公开(公告)号：US08959026B2
公开(公告)日：2015-02-17
申请号：US13126947
申请日：2009-10-28
申请人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
发明人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
IPC分类号： G10L19/00 , G10L19/008
CPC分类号： G10L19/008
摘要： An apparatus and method for encoding/decoding a multi-channel signal may be provided. The apparatus of encoding a multi-channel signal may insert information about whether to encode a phase parameter indicating phase information of a plurality of channels, included in the multi-channel signal, in a bitstream of the multi-channel signal. The apparatus of decoding a multi-channel signal may determine whether to up-mix a mono signal using the phase parameter based on the information about whether to encode.
摘要翻译：可以提供用于对多信道信号进行编码/解码的装置和方法。编码多声道信号的装置可以在多声道信号的比特流中插入关于是否编码指示包括在多声道信号中的多个声道的相位信息的相位参数的信息。解码多声道信号的装置可以基于关于是否编码的信息，使用相位参数来确定是否对单声道信号进行混合。

5. 发明授权

US08452018B2 Apparatus and method for encoding/decoding multichannel signal using phase information 有权
标题翻译：使用相位信息对多通道信号进行编码/解码的装置和方法
公开(公告)号：US08452018B2
公开(公告)日：2013-05-28
申请号：US13483954
申请日：2012-05-30
申请人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
发明人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
IPC分类号： H04R5/00
CPC分类号： G10L19/008
摘要： An apparatus and method for encoding/decoding a multi-channel signal may be provided. The apparatus of encoding a multi-channel signal may insert information about whether to encode a phase parameter indicating phase information of a plurality of channels, included in the multi-channel signal, in a bitstream of the multi-channel signal. The apparatus of decoding a multi-channel signal may determine whether to up-mix a mono signal using the phase parameter based on the information about whether to encode.
摘要翻译：可以提供用于对多信道信号进行编码/解码的装置和方法。编码多声道信号的装置可以在多声道信号的比特流中插入关于是否编码指示包括在多声道信号中的多个声道的相位信息的相位参数的信息。解码多声道信号的装置可以基于关于是否编码的信息，使用相位参数来确定是否对单声道信号进行混合。

6. 发明申请

US20090306971A1 AUDIO SIGNAL QUALITY ENHANCEMENT APPARATUS AND METHOD 有权
标题翻译：音频信号质量增强装置和方法
公开(公告)号：US20090306971A1
公开(公告)日：2009-12-10
申请号：US12479009
申请日：2009-06-05
申请人： Jung Hoe Kim , Ho Chong Park , Eun Mi Oh
发明人： Jung Hoe Kim , Ho Chong Park , Eun Mi Oh
IPC分类号： G10L21/00 , G10L19/02
CPC分类号： G10L21/0364
摘要： An audio signal quality enhancement apparatus and method. The apparatus includes a pitch calculating unit to extract a pitch period of an audio signal, a frequency domain transforming unit to transform the audio signal to a frequency domain, a frequency band dividing unit to classify the transformed audio signal into audio signals for each of the plurality of frequency bands based on the extracted pitch period, and a pitch enhancement unit to determine a gain based on a volume of the transformed audio signal, and to generate an output signal by multiplying each of the classified audio signals with respect to each of the plurality of frequency bands by the gain, thereby enhancing quality of the audio signal.
摘要翻译：一种音频信号质量增强装置和方法。该装置包括：音调计算单元，用于提取音频信号的音调周期;频域变换单元，用于将音频信号变换到频域;频带分割单元，用于将经变换的音频信号分类为音频信号，基于所提取的音调周期的多个频带，以及音调增强单元，用于基于经变换的音频信号的音量来确定增益，并且通过将每个分类的音频信号相对于每个音频信号相乘来生成输出信号通过增益获得多个频带，从而提高音频信号的质量。

7. 发明申请

US20120221343A1 APPARATUS AND METHOD FOR ENCODING/DECODING A MULTICHANNEL SIGNAL 有权
公开(公告)号：US20120221343A1
公开(公告)日：2012-08-30
申请号：US13464509
申请日：2012-05-04
申请人： Mi Young KIM , Jung Hoe Kim , Hwan Shim , Eun MI OH
发明人： Mi Young KIM , Jung Hoe Kim , Hwan Shim , Eun MI OH
IPC分类号： G10L19/00
CPC分类号： G10L19/008
摘要： An apparatus for encoding/decoding a multichannel signal. The apparatus for encoding/decoding a multichannel signal processes phase parameters for phase information among a plurality of channels constituting the multichannel signal in consideration of the characteristics of the multichannel signal. The apparatus generates an encoded bit stream for the multichannel signal using the processed phase parameters and the mono signal extracted from the multichannel signal.

8. 发明申请

US20120010891A1 APPARATUS AND METHOD FOR ENCODING/DECODING MULTICHANNEL SIGNAL 有权
公开(公告)号：US20120010891A1
公开(公告)日：2012-01-12
申请号：US13126947
申请日：2009-10-28
申请人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
发明人： Jung Hoe Kim , Eunmi Oh , Mi Young Kim , Ki Hyun Choo
IPC分类号： G10L19/00
CPC分类号： G10L19/008
摘要： An apparatus and method for encoding/decoding a multi-channel signal may be provided. The apparatus of encoding a multi-channel signal may insert information about whether to encode a phase parameter indicating phase information of a plurality of channels, included in the multi-channel signal, in a bitstream of the multi-channel signal. The apparatus of decoding a multi-channel signal may determine whether to up-mix a mono signal using the phase parameter based on the information about whether to encode.

9. 发明申请

US20110103592A1 Apparatus and method encoding/decoding with phase information and residual information 有权
标题翻译：具有相位信息和残差信息的装置和方法编码/解码
公开(公告)号：US20110103592A1
公开(公告)日：2011-05-05
申请号：US12926054
申请日：2010-10-22
申请人： Jung Hoe Kim , Eun Mi Oh
发明人： Jung Hoe Kim , Eun Mi Oh
IPC分类号： H04R5/00
CPC分类号： G10L19/008 , G10L19/12 , G10L19/167 , G10L19/22 , G10L21/038 , H04N21/4382
摘要： Provided is an apparatus and method of encoding and decoding multiple channel signals based upon phase information and one or more residual signals.
摘要翻译：提供了一种基于相位信息和一个或多个残余信号对多个信道信号进行编码和解码的装置和方法。

10. 发明授权

US07820414B2 Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, method of producing high-yield of xylitol using the same, and xylitol produced thereby 有权
标题翻译：木糖醇脱氢酶灭活和热带假丝酵母的阿拉伯糖还原酶抑制突变体，使用其产生高产量的木糖醇的方法和由此生产的木糖醇
公开(公告)号：US07820414B2
公开(公告)日：2010-10-26
申请号：US11771081
申请日：2007-06-29
申请人： Jung Hoe Kim , Byoung Sam Ko
发明人： Jung Hoe Kim , Byoung Sam Ko
IPC分类号： C12P7/18 , C12P19/02 , C12N1/10
CPC分类号： C12N1/16 , C12P7/18
摘要： Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.
摘要翻译：本文公开了热带假丝酵母的木糖醇脱氢酶失活和阿拉伯糖还原酶抑制突变体，使用其产生高产率的木糖醇的方法，以及通过该方法生产的木糖醇。更具体地，公开了一种生产高产率的木糖醇的方法，其中将生物质水解产物中所含的高浓度的木糖转化为木糖醇，使用热带假丝酵母的木糖醇脱氢酶灭活的突变体，而不将溶解氧控制在低水平，以及根据该方法生产的木糖醇。还公开了一种木糖醇生产方法，其中通过使用热带假丝酵母突变体ara-89（KCTC 11136bp）显着降低在使用生物质作为底物时产生的副产物阿拉伯糖醇的产生并且不利地影响木糖醇的产率）具有抑制阿拉伯糖还原酶的活性，其将阿拉伯糖转化成阿糖醇，从而增加木糖醇生产率，以及通过该方法产生的木糖醇。

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