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1. 发明专利

JPS619282A Method for cultivating genetic recombinant microorganism 失效
标题翻译：用于培养遗传重组微生物的方法
公开(公告)号：JPS619282A
公开(公告)日：1986-01-16
申请号：JP12747284
申请日：1984-06-22
申请人： Hitachi Ltd
发明人： SHIMIZU NORIO , MASUDA KEIKO , KURIHARA TOSHIHARU , OTAHARA YOUJI
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/38 , C12N15/00 , C12R1/19
摘要： PURPOSE: To obtain efficiently a product of the titled microorganism in a large amount, by cultivating a microorganism having a complex plasmid consisting of the aimed gene, vector and promoter and the expression ability thereof in a culture medium containing an inhibitor substance and an inducer substance to control the promoter activity.
CONSTITUTION: A microorganism having the aimed gene, vector and promoter in the cell and an expression ability of the aimed gene is cultivated in a culture medium containing an inhibitor substance and inducer substance to control the promoter activity and give the product of the aimed gene efficiently in a large amount. For example, a DNA fragment containing a trp promoter cut out of PTREI is linked to an β-gal gene with an enzyme to create a complex plasmid PTREZI, and tryptophan (trp) is added thereto in the initial period of cultivation to suppress the activity of the trp promoter, reduce the production of the β-gal and multiply the microbial cells. An inducer substance for starting the activity of the trp promoter is then added to produce the β-gal.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过培养具有目标基因，载体和启动子组成的复合质粒的微生物及其在含有抑制物质和诱导物质的培养基中的表达能力，有效地获得大量标题微生物的产物以控制启动子活性。构成：在含有抑制剂物质和诱导物质的培养基中培养具有目的基因，细胞中的载体和启动子和靶基因的表达能力的微生物，以控制启动子活性并有效地产生目的基因的产物大量的。例如，含有从PTREI切出的trp启动子的DNA片段与酶的β-gal基因连接以产生复合质粒PTREZI，并且在培养的初始阶段向其中加入色氨酸（trp）以抑制活性的trp启动子，减少β-gal的产生并繁殖微生物细胞。然后加入用于启动trp启动子活性的诱导物质以产生β-gal。

2. 发明专利

JPS59140877A Immobilized enzyme reactor 失效
标题翻译：固定化酶反应器
公开(公告)号：JPS59140877A
公开(公告)日：1984-08-13
申请号：JP1128783
申请日：1983-01-28
申请人： Hitachi Ltd
发明人： YAMAGUCHI TETSUO , OTAHARA YOUJI
IPC分类号： C12M1/40
摘要： PURPOSE: To prevent the increase of pressure drop in the packed layer of an immobilized enzyme reactor, by placing a reaction zone packed with immobilized enzyme in a reactor in plural layers divided along the flow direction of the substrate solution, and attaching a means for controlling the temperature, pH, etc. of the substrate solution between the reaction layers.
CONSTITUTION: The inside of an immobilized enzyme reactor 4 is divided into the alternately arranged plural reaction layers 5 packed with immobilized enzyme and the controlling parts 6, and the reticular supporting material 7 for preventing the escape of the immobilized enzyme packed in the reaction chamber 5 is placed between the controlling parts 6 and the reaction layers 5. The controlling part 6 is furnished with the pipes 8, 10 to transfer the reaction liquid flowed down from the upstream-side reaction layer 5 to the downstream-side reaction layer 5. A mixing part is placed between the pipes 8 and 10, and an inlet 11 of a pH-controlling liquid is attached in the mixing part 9, and the inlet 13 and the outlet 14 of the medium for the temperature control of the reaction liquid is attached to the mixing part 9.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：为了防止固定化酶反应器的填充层压降的增加，通过将填充有固定化酶的反应区域放置在沿着基板溶液的流动方向分割的多层的反应器中，并且附接控制装置反应层之间的底物溶液的温度，pH等。构成：将固定化酶反应器4的内部分成交替排列的填充有固定化酶的多个反应层5和控制部6以及用于防止包装在反应室5中的固定化酶逸出的网状支撑材料7 配置在控制部6和反应层5之间。控制部6配备有管8,10，以将从上游侧反应层5向下流动的反应液转印到下游侧反应层5。将混合部件放置在管道8和10之间，并且将pH值控制液体的入口11附接在混合部分9中，并且连接用于反应液体的温度控制的介质的入口13和出口14 到混合部分9。

3. 发明专利

JPS58201976A Colony transfer device 失效
标题翻译：殖民地转移装置
公开(公告)号：JPS58201976A
公开(公告)日：1983-11-25
申请号：JP8401182
申请日：1982-05-20
申请人： Hitachi Electronics Eng Co Ltd , Hitachi Ltd
发明人： SHIBAYAMA KATSUJIROU , IWATANI FUKUO , TAKAHASHI KENSAKU , IKUMI MASUZOU , TSUJI TATSUTO , OOKUMA MICHIO , OTAHARA YOUJI
IPC分类号： C12M1/26 , C12M1/00
CPC分类号： C12M33/06
摘要： PURPOSE: To transplant a colony in a laboratory dish for test, by selecting the colony in a laboratory dish for culture by a monitor part with an eye, operating a pickup part under control of a microcomputer equipped with a memory and an operation panel.
CONSTITUTION: The laboratory dish for culture 11 and the laboratory dish for test 15 are placed on the X and Y stages 19-1 and 19-2 at both the ends of the common base 18, and a colony to be transplanted on the laboratory dish for culture 11 is selected by the monitor part 22. It is input to the microcomputer 23 equipped with the memory 24 and the operation panel 25 by the monitor part 22. The pickup part 10, the pickup treatment part 21 and the transfer part 20 are removed under control of the microcomputer 23, and the colony on the laboratory dish for culture 11 is transplanted in the laboratory dish for test 15.
COPYRIGHT: (C)1983,JPO&Japio
摘要翻译：目的：将实验室中的菌落移植到实验室进行测试，通过用眼睛的监视器部分选择用于培养的实验室培养皿中的菌落，在装有存储器和操作面板的微型计算机的控制下操作拾取部件。规定：用于培养的实验室11和用于测试15的实验室培养皿放置在共同基部18的两端的X和Y阶段19-1和19-2上，并将待移植到实验室皿上的菌落用于培养物11由监视器部分22选择。通过监视器部分22输入到配有存储器24和操作面板25的微型计算机23.拾取部分10，拾取处理部分21和转印部分20是在微型计算机23的控制下移除，并将用于培养的实验室皿11上的菌落移植到实验室中用于测试15。

4. 发明专利

JPS5722695A Pretreatment of cellulosic material 失效
标题翻译：纤维素材料的预处理
公开(公告)号：JPS5722695A
公开(公告)日：1982-02-05
申请号：JP9751780
申请日：1980-07-18
申请人： Hitachi Ltd
发明人： ISHIBASHI HITOSHI , ISHIDA MASAHIKO , OTAHARA YOUJI
IPC分类号： C12N1/22 , C12P19/14
摘要： PURPOSE: To improve the biological decomposition properties of the titled material, by contacting the titled material with ozone under wet state, immersing in an aqueous solution of phosphoric acid, and washing with water.
CONSTITUTION: The cellulosic material 1 used as a raw material, e.g. straw, bagasse, waste paper, etc., is crushed with a crusher 2, and mixed in a mixer 3 with water supplied from the water tank 4. The wet cellulosic material 1 is then made to contact in an ozone reactor 5 with ozone generated from the ozonizer 6, and treated with phosphoric acid in the phosphoric acid treating tank 7. After the phosphoric acid treatment, the cellulosic material 1 is washed with water 9 and separated in the washing and separating apparatus 8.
COPYRIGHT: (C)1982,JPO&Japio
摘要翻译：目的：为了提高标题材料的生物分解性能，通过将标题材料与臭氧在湿态下接触，浸入磷酸水溶液中，并用水洗涤。构成：用作原料的纤维素材料1，例如秸秆，甘蔗渣，废纸等用破碎机2粉碎，并在混合器3中与从水箱4供应的水混合。然后使湿的纤维素材料1在臭氧反应器5中与产生的臭氧接触并且在磷酸处理槽7中用磷酸处理。磷酸处理后，纤维素材料1用水洗涤9，并在洗涤分离装置8中分离。

5. 发明专利

JPS5716690A PRETREATING METHOD OF CELLULOSIC MATERIAL 失效
公开(公告)号：JPS5716690A
公开(公告)日：1982-01-28
申请号：JP8916680
申请日：1980-07-02
申请人： HITACHI LTD
发明人： ISHIBASHI HITOSHI , ISHIDA MASAHIKO , OTAHARA YOUJI
IPC分类号： C02F3/28 , A23K10/32 , C12N1/00
摘要： PURPOSE:To improve the biodegradability of cellulose, by ozonizing a cellulosic material, mixing the ozonized cellulosic material with an aqueous solution of an alkaline metal or alkaline earth metal, and heating the mixture at about 100 deg.C. CONSTITUTION:The moisture content of a raw cellulosic material is increased to 10% or more in a mixing tank 1 with water supplied from a water tank 3 by an atomizer 2, and brought into contact with ozone supplied from an ozonizer 5 in an ozone treating tank 4 by the gas-solid contacting method. The treated cellulosic material is then discharged from a discharge feeder 6 at the bottom of the tank 4. A 0.05-0.8wt% aqueous solution of an alkali, e.g. NaOH solution, in an amount of 0.5- 20 times that of the cellulosic material is fed from an alkali storage tank 7 and sprayed on the cellulosic material in a mixing heating tank 8 by the atomizer 2, and the sprayed cellulosic material is then heated at about 100 deg.C.

6. 发明专利

JPS5716656A PREPARATION OF ROUGHAGE 失效
公开(公告)号：JPS5716656A
公开(公告)日：1982-01-28
申请号：JP8916580
申请日：1980-07-02
申请人： HITACHI LTD
发明人： ISHIBASHI HITOSHI , ISHIDA MASAHIKO , OTAHARA YOUJI
IPC分类号： A23K1/00 , A23K1/12
摘要： PURPOSE:To improve the digestibility of roughage and to enable the utilization of lignin in the preparation of roughage, by impregnating cellulosic materials with water or an aqueous solution of caustic soda, and contacting the treated cellulosic materials with ozone. CONSTITUTION:Cellulosic materials 1, e.g. straw such as rice straw, bagasse, corn stover, etc. are crushed with a crusher 2, and mixed in a mixing tank 3 with water or an aqueous solution of caustic soda supplied from the solution tank 4. The cellulosic materials discharged from the mixing tank 3 are fed to the ozone reactor 5, and brought into contact with ozone generated in an ozone generator 6.

7. 发明专利

JPS56144086A CULTIVATION OF MICROORGANISM IN HIGH CONCENTRATION OF MOLD 失效
公开(公告)号：JPS56144086A
公开(公告)日：1981-11-10
申请号：JP4563680
申请日：1980-04-09
申请人： HITACHI LTD
发明人： SHIMIZU NORIO , YAMAGUCHI TETSUO , SAITOU SETSUO , UENO MASAO , OTAHARA YOUJI
IPC分类号： C12N1/00 , C12R1/78 , C12R1/865
摘要： PURPOSE:To cultivate molds in high concentration without causing contamination resulting from various germs and to intend rise in yield of mold, by returning partly molds as seed molds prepared from processes after washing process after cultivation to a culture tank. CONSTITUTION:A medium and oxygen are fed to the culture bath 1 and the cultivation of microorganisms is carried out aerobically with stirring. Molds are sent to the centrifugal separator 6, washed with cleaning water and separated, concentrated by the filter press 11, and dried to give a product. The molds prepared from the processes after washing process as seed microorganisms are partly returned to the cultivation tank 1 by the return pipe 10. Thus cultivation with an initial concentration >=20g-dry cell/l is carried out. This method is used for cultivation of various kinds of yeast, bacilli, molds, and ray fungi.

8. 发明专利

JPS55129195A FILAMENTOUS BACTERIA EXTERMINATING METHOD IN ACTIVATED SLUDGE TREATMENT 失效
公开(公告)号：JPS55129195A
公开(公告)日：1980-10-06
申请号：JP3556079
申请日：1979-03-28
申请人： HITACHI LTD
发明人： SAITOU SETSUO , SHIMIZU NORIO , OTAHARA YOUJI
IPC分类号： C02F3/12
摘要： PURPOSE:To recover the settleability of activated sludge by raising the osmotic press. of liquor in an aeration tank to 5 atm or more to exterminate filamentous bacteria propagated in the sludge. CONSTITUTION:By adding salts such as table salt or sodium sulfate to water treated with activated carbon whose settleability has been deteriorated by propagation of filamentous bacteria, the osmotic press. of the water is raised to 5 atm or more to exterminate the bacteria. When the amt. of the salts added is known, the osmotic press. is obtd. almost approximately (dissolvable org. constituents are neglected) according to the following van't Hoff's equation of osmotic press.: pi=i.R.T.C (where pi is osmotic press., i is the van't Hoff's factor, R is the gas constant, T is the absolute temp. and C is the solute mol no.). When the concn., etc. of the salts in the water are not clear, the amt. of the salts to be added can be adjusted with an osmometer so that the water becomes the predetermined osmotic press.

9. 发明专利

JPS54150850A METHOD AND DEVICE FOR DISPOSING WASTE WATER BY USE OF ACTIVE SLUDGE 失效
公开(公告)号：JPS54150850A
公开(公告)日：1979-11-27
申请号：JP5764978
申请日：1978-05-17
申请人： HITACHI LTD
发明人： MAZAKI YASUNORI , SHIMIZU NORIO , OTAHARA YOUJI , MIYAZAKI KAZUO , SUGIMOTO KUNJI
IPC分类号： C02F3/12
摘要： PURPOSE:To dispose effectively the waste water of a high BOD by setting the oxygen moving speed under the initial exposure condition at a predetermined value or more and dropping the speed down successively. CONSTITUTION:The waste water of a high BOD of which the BOD density is more than 2000 ppm, not diluted, is subjected to the air exposure in a primary air exposing vessel at an exygen moving speed (OTR value) of more than 20 m mol. O2/l.hr, thereby conducting a microorganism reaction sufficiently. If 50% of the BOD is preferably removed here, a highly active sludge which has been enriched under the high OTR value becomes prominent, which facilitates the subsequent disposal. Several air exposing vessels having successively reduced OTR values in accordance with the BOD density are provided, through which the waste water has lost BOD sufficiently when it is introduced finally into a sludge separating vessel. According to the present method, the power consumption per BOD can be minimized very much.

10. 发明专利

JPS54133755A METHOD OF PROCESS FOR WASTE WATER 失效
公开(公告)号：JPS54133755A
公开(公告)日：1979-10-17
申请号：JP4122278
申请日：1978-04-10
申请人： HITACHI LTD
发明人： NISHIMURA YUUSAKU , ISHIBASHI HITOSHI , KUBOTA SHIYOUJI , OTAHARA YOUJI , TAKAHASHI SANKICHI
IPC分类号： C02F1/42 , C02F3/34
摘要： PURPOSE:To process waste water econimically with out adding organic substances as nourishment by effecting positive ion-exchange for a part of waste water including organic substances and nitrogen oxide and introducing processed water into denitrifying process while positive ion-exchange substances into nitrifying process. CONSTITUTION:When a part of waste water 1 including organic substances and ammonium, nitrous or nitric nitrogen is sent to a positive ion-exchange process tank 7 and is contacted with positive ion-exchange substances such as zeolit, the ammonium nitrogen in the waste water 1 is removed by positive ion-exchange. Thereafter, this processed water is sent to a denitrifying tank 5 through a settling tank 9 while the substance, which absorbed the ammonium nitrogen separated in the settling tank 9 and procesed by the positive ion exchange, is sent to a nitrifying tank 4. Then, denitrifying bacteria are activated by nourishment of organic substances in the processed water in the denitrifying tank 5 and the positive ion-exchange substances in the nitrifying tank 4 are revived and circulated to the positive ion-exchange process tank 7 and thus reused.

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