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    • 6. 发明专利
    • LEUKOCYTE ANALYZING METHOD
    • JPH0599919A
    • 1993-04-23
    • JP9055991
    • 1991-04-22
    • HITACHI LTDHITACHI INSTRUMENTS ENG
    • HORIUCHI HIDEYUKIYABE RYOHEISAKURABA SHINICHIKANEKO NORIOTATARA NOBUYUKIOKI HIROSHIYAMAZAKI ISAOMIYAKE AKIRA
    • G01N15/14G01N33/48G01N33/49
    • PURPOSE:To realize the classification of five kinds of the normal leukocytes by using acridine orange fluorescence staining solution containing acridine orange fluorescence dyestuff, buffer solution whose pH is higher than physiological pH and physiological isotonic osmotic pressure reagent as analyzing fluorescence staining solution. CONSTITUTION:When leukocyte is stained with acridine orange staining agent, the leukocyte is bonded with neucleuses present in the leukocyte and granules present in cytoplasma. The green or red fluorescence is generated. The neucleus of the cell is mainly constituted of DNA. When the neucleus is bonded with the acridine orange, the green fluorescence indicating the maximum value in the vicinity of 530nm is generated. The granule present in the cytoplasma contains RNA. Therefore, the red fluorescence having the maximum value in the vicinity of 640nm is generated. The osmotic pressure of the acridine orange fluorescence staining solution is set in the vicinity of 290mosm/kg of physiological osmotic pressure. The pH of fluorescence solution is set at a value higher than the normal physiological pH. Thus, the five of the normal leukocytes can be classified. Furthermore, a small amount of erythrocyte hemolysis agent is added into the staining solution. Thus, the surface of the cell cytoplasmic membrane of the leukocyte is changed. In this way, the fluoresence intensity is increased at normal temperature and the staining time is shortened.
    • 10. 发明专利
    • FLOW CELL DEVICE
    • JPH0298653A
    • 1990-04-11
    • JP24985888
    • 1988-10-05
    • HITACHI LTD
    • OKI HIROSHIMIYAKE AKIRAYAMAZAKI ISAOHORIUCHI HIDEYUKIKANEKO NORIOSAKURABA SHINICHIYASUDA KAORI
    • G01N21/05G01N27/02
    • PURPOSE:To prevent carry-over of a sample liquid even if there is no back sheath by providing a sample passage which is opened so that a part of a sheath passage is left in the upper and the lower parts of its opening part, in the sheath passage. CONSTITUTION:A sheath fluid 4 is led into a flow cell device 1 from two inlets 3. The sheath fluid 4 is brought to contraction in a passage 5, and flows into a constricted passage 6 in a laminar flow state. On the other hand, a sample fluid 9 flows into the device 1 from an inlet 8. The sample fluid 9 is brought to contraction in a passage 10, and flows into a confluence point 11 of the passage 5. In this case, since a part of the passage 5 is left in the upper and the lower parts of an opening 12, the sample fluid 9 is surrounded by the sheath fluid 4 from not only the right and the left directions but also the upper and the lower directions, becomes a thin flow 14 on the cross section center of the constricted passage 6 and flows. Since the thin flow 14 is a laminar flow, a particle selected by the sample fluid 4, for instance, a cell flows into a suspension one by one.