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1. 发明授权

US08789495B2 Method and apparatus for conserving deep-sea organisms 有权
标题翻译：保护深海生物的方法和装置
公开(公告)号：US08789495B2
公开(公告)日：2014-07-29
申请号：US13319182
申请日：2010-06-14
申请人： Guenter R. Fuhr , Heiko Zimmermann , Charli Kruse , Robert Lemor
发明人： Guenter R. Fuhr , Heiko Zimmermann , Charli Kruse , Robert Lemor
IPC分类号： A01K63/02
CPC分类号： A01K63/02 , A01N1/00 , A01N1/0221 , A01N1/0252 , A01N1/0284
摘要： The invention relates to a method for conserving at least one biological deep-sea organism (1) comprising the steps of collecting the biological organism (1) in a deep-sea trapping device (110); cooling the biological organism (1) with a cooling device (120), wherein the biological organism (1) is put in a frozen condition; and moving the frozen biological organism (1), preferably with the trapping device (110), to a normal-pressure environment. The invention further relates to a conservation apparatus (100) for conserving at least one biological deep-sea organism (1).
摘要翻译：本发明涉及一种用于保存至少一种生物深海生物体的方法（1），包括以下步骤：在深海捕获装置（110）中收集生物体（1）; 用冷却装置（120）冷却生物体（1），其中所述生物体（1）处于冷冻状态; 以及将所述冷冻生物体（1）优选地与所述捕集装置（110）一起移动到常压环境。本发明还涉及一种用于保存至少一种生物深海生物（1）的保存装置（100）。

2. 发明申请

US20120079990A1 METHOD AND APPARATUS FOR CONSERVING DEEP-SEA ORGANISMS 有权
标题翻译：用于保护深海有机体的方法和装置
公开(公告)号：US20120079990A1
公开(公告)日：2012-04-05
申请号：US13319182
申请日：2010-06-14
申请人： Guenter R. Fuhr , Heiko Zimmermann , Charli Kruse , Robert Lemor
发明人： Guenter R. Fuhr , Heiko Zimmermann , Charli Kruse , Robert Lemor
IPC分类号： A01N1/02 , A01M23/00 , A01K61/00
CPC分类号： A01K63/02 , A01N1/00 , A01N1/0221 , A01N1/0252 , A01N1/0284
摘要： The invention relates to a method for conserving at least one biological deep-sea organism (1) comprising the steps of collecting the biological organism (1) in a deep-sea trapping device (110); cooling the biological organism (1) with a cooling device (120), wherein the biological organism (1) is put in a frozen condition; and moving the frozen biological organism (1), preferably with the trapping device (110), to a normal-pressure environment. The invention further relates to a conservation apparatus (100) for conserving at least one biological deep-sea organism (1).
摘要翻译：本发明涉及一种用于保存至少一种生物深海生物体的方法（1），包括以下步骤：在深海捕获装置（110）中收集生物体（1）; 用冷却装置（120）冷却生物体（1），其中所述生物体（1）处于冷冻状态; 以及将所述冷冻生物体（1）优选地与所述捕集装置（110）一起移动到常压环境。本发明还涉及一种用于保存至少一种生物深海生物（1）的保存装置（100）。

3. 发明申请

US20110020933A1 METHOD AND DEVICE FOR RECEIVING BIOLOGICAL CELLS FROM A STEM CELL CULTURE 审中-公开
标题翻译：用于从干细胞培养物接收生物细胞的方法和装置
公开(公告)号：US20110020933A1
公开(公告)日：2011-01-27
申请号：US12671534
申请日：2008-07-25
申请人： Charli Kruse , Guenter R. Fuhr
发明人： Charli Kruse , Guenter R. Fuhr
IPC分类号： C12N5/074
CPC分类号： C12M33/14 , C12M33/00
摘要： A method for receiving biological cells (1, 2) from a cell culture (3) includes the steps: cultivation of the cell culture on a main substrate (10), cell transfer from the main substrate (10) onto at least one receiving substrate (20, 20.1, 20.2, 20.3, 20.4 . . . ), wherein the cells (1, 2) move onto the at least one receiving substrate (20, 20.1, 20.2, 20.3, 20.4 . . . ) by natural cell locomotion, and a cultivation of the cells (1, 2) is provided on the at least one receiving substrate (20, 20.1, 20.2, 20.3, . . . ), and separation of the main substrate (10) and of the at least one receiving substrate (20, 20.1, 20.2, 20.3, 20.4 . . . ) from one another. Also described is a substrate arrangement (100) for providing biological cells (1, 2) of a cell culture (3).
摘要翻译：从细胞培养物（3）接收生物细胞（1,2）的方法包括以下步骤：在主衬底（10）上培养细胞培养物，将细胞从主衬底（10）转移到至少一个接收衬底（20,20.1,20.2,20.3,20.4 ...），其中细胞（1,2）通过天然细胞运动移动到至少一个接收衬底（20,20.1,20.2,20.3,20.4 ...）上，并且在所述至少一个接收基板（20,20.1,20.2,20.3等）上设置所述单元（1,2）的培养，并且分离所述主基板（10）和所述至少一个接收衬底（20,20.1,20.2,20.3,20.4 ...）彼此。还描述了一种用于提供细胞培养物（3）的生物细胞（1,2）的底物装置（100）。

4. 发明授权

US08298822B2 Method for the production of a cell composition containing epithelial cells 有权
标题翻译：用于生产含有上皮细胞的细胞组合物的方法
公开(公告)号：US08298822B2
公开(公告)日：2012-10-30
申请号：US11547678
申请日：2005-04-01
申请人： Charli Kruse , Gunter Fuhr , Thilo Wedel
发明人： Charli Kruse , Gunter Fuhr , Thilo Wedel
IPC分类号： C12N5/00 , C12N5/02 , C12N5/071 , A01N63/00
CPC分类号： C12N5/0677 , A61K35/24 , A61K35/26
摘要： Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.
摘要翻译：公开了形成上皮细胞的方法。所述方法包括以下步骤：从分化的外分泌腺组织聚集干细胞以获得有机体，并分化至少一部分有机体或由其生长的组织体以获得上皮细胞。还公开了一种培养装置，特别是用于形成差异上皮细胞的培养装置。

5. 发明申请

US20100184621A1 METHOD AND DEVICE FOR FORMING BIOLOGIC CELL AGGREGATES 有权
标题翻译：用于形成生物细胞聚集体的方法和装置
公开(公告)号：US20100184621A1
公开(公告)日：2010-07-22
申请号：US12665325
申请日：2008-06-13
申请人： Charli Kruse , Sandra Danner
发明人： Charli Kruse , Sandra Danner
IPC分类号： C40B30/06 , C12N5/00 , C12N5/0789 , C12M1/00
CPC分类号： C12N5/0062 , C12M21/08 , C12M25/14
摘要： The invention relates to a method for cultivating biologic cells (1), wherein cells (1) are grown on a substrate (10) having a plurality of substrate openings (11), and wherein cell aggregates (2) are formed, including groups of cells (1) that span the substrate openings (11). A separation of the cell aggregates (2) from the substrate (10) by extracting the cell aggregates (2) from the substrate openings (11) can be provided. The invention further relates to a cell-cultivating device (100), including a substrate (10) having a plurality of substrate openings (11) and cell aggregates (2) including groups of cells (1) that span the substrate openings (11). The cell aggregates (2) are particularly used in high throughput tests with biologically active substances or in methods of tissue cultivation.
摘要翻译：本发明涉及一种培养生物细胞的方法（1），其中细胞（1）在具有多个基底开口（11）的基底（10）上生长，并且其中形成细胞聚集体（2），包括跨越衬底开口（11）的电池（1）。可以提供通过从基板开口（11）提取细胞聚集体（2）而将细胞聚集体（2）与基底（10）分离。本发明还涉及一种细胞培养装置（100），其包括具有多个基板开口（11）的基板（10）和包括跨越基板开口（11）的电池组（1）的电池集合体（2）。细胞聚集体（2）特别用于具有生物活性物质或组织培养方法的高通量测试中。

6. 发明申请

US20090220565A1 METHOD FOR PRODUCING AUTONOMOUSLY CONTRACTING CARDIAC MUSCLE CELLS FROM ADULT STEM CELLS, IN PARTICULAR HUMAN ADULT STEM CELLS 审中-公开
标题翻译：用于生产来自成熟干细胞的自体合成的心脏肌肉细胞的方法，特别是人成熟干细胞
公开(公告)号：US20090220565A1
公开(公告)日：2009-09-03
申请号：US12162077
申请日：2007-01-26
申请人： Norbert W. Guldner , Charli Kruse , Jennifer Kajahn
发明人： Norbert W. Guldner , Charli Kruse , Jennifer Kajahn
IPC分类号： A61K35/34 , C12N5/06 , C12N5/08 , A61F2/02 , A61P9/00
CPC分类号： A61L27/3895 , A61K35/12 , A61L27/24 , A61L27/3834 , A61L27/3873 , A61L27/56 , C12N5/0657 , C12N5/0678 , C12N2501/06 , C12N2501/12 , C12N2502/1329 , C12N2506/22
摘要： A method for producing autonomously contractile heart muscle cells by cultivating and differentiating stem cells obtained from differentiated exocrine gland tissue of an organism is described. Various uses of the heart muscle cells, in particular in regenerative medicine, are also described.
摘要翻译：描述了通过培养和分化从生物体的分化的外分泌腺组织获得的干细胞来产生自主收缩的心肌细胞的方法。还描述了心肌细胞，特别是再生医学中的各种用途。

7. 发明授权

US08603809B2 Isolated adult pluripotent stem cells and methods for isolating and cultivating thereof 有权
公开(公告)号：US08603809B2
公开(公告)日：2013-12-10
申请号：US10820430
申请日：2004-04-08
申请人： Charli Kruse
发明人： Charli Kruse
IPC分类号： C12N5/00 , C12N5/02
CPC分类号： C12N5/0607 , A61K35/12 , A61K35/39
摘要： This invention relates to isolated pluripotent adult stem cells which are obtained from exocrine glandular tissue as well as methods of isolating and culturing these pluripotent stem cells.

8. 发明申请

US20100319079A1 ISOLATED PROLIFERATING CELLS WITH STEM CELL PROPERTIES FROM ADULT TISSUE OF POIKILOTHERMIC VERTEBRATES, STABLE CELL CULTURES THEREOF, AND METHODS FOR THEIR PREPARATION 审中-公开
标题翻译：具有干细胞特性的分离的增殖细胞，其耐药性纤维细胞培养物及其制备方法及其制备方法
公开(公告)号：US20100319079A1
公开(公告)日：2010-12-16
申请号：US12664916
申请日：2008-02-27
申请人： Charli Kruse , Emel Singh
发明人： Charli Kruse , Emel Singh
IPC分类号： A01K67/00 , C12N5/071 , C12N9/00 , C12P7/64 , G01N33/53
CPC分类号： C12N5/0602 , C12N2503/02
摘要： A method of preparing adult proliferating cells with stem cell properties includes removing tissue from pronephros or pyloric appendates of an intestine of poikilothermic vertebrates, comminuting removed tissue, cultivating comminuted tissue, and propagating cells persisting in a culture.
摘要翻译：制备具有干细胞特性的成体增殖细胞的方法包括从前列腺或幽门螺旋体脊椎动物肠的幽门阑尾去除组织，粉碎去除的组织，培养粉碎的组织和繁殖培养物中的细胞。

9. 发明申请

US20070248716A1 Method For the Production of a Biological Material Composition Having an Animal Origin 审中-公开
标题翻译：用于生产具有动物来源的生物材料组合物的方法
公开(公告)号：US20070248716A1
公开(公告)日：2007-10-25
申请号：US10599713
申请日：2005-03-04
申请人： Charli Kruse , Gunter Fuhr , Jurgen Rohwedel
发明人： Charli Kruse , Gunter Fuhr , Jurgen Rohwedel
IPC分类号： A23K1/00 , A23L1/00 , C12N5/02 , C12N5/06
CPC分类号： C12N5/0677
摘要： A method for producing a biological material composition of animal, non-human origin, especially as feed or food, is described. The method includes an aggregation of stem cells from differentiated exocrine glandular tissue of an animal organism to organoid bodies, and a preparation of the material composition from the organoid bodies.
摘要翻译：描述了用于生产动物，非人来源的生物材料组合物的方法，特别是作为饲料或食物。该方法包括将来自动物生物体的分化的外分泌腺体组织的干细胞与组织体的聚集，以及来自有机体的物质组合物的制备。

10. 发明申请

US20070037281A1 Method for differentiating stem cells in cells that produce a pancreatic hormone 审中-公开
标题翻译：在产生胰腺激素的细胞中分化干细胞的方法
公开(公告)号：US20070037281A1
公开(公告)日：2007-02-15
申请号：US10561628
申请日：2004-06-23
申请人： Charli Kruse
发明人： Charli Kruse
IPC分类号： C12N5/08 , C12N15/09
CPC分类号： C12N5/0677 , A61K35/12 , C12N2502/22 , C12N2509/00 , C12N2533/54
摘要： Described is a process for generating cells producing pancreatic hormone by cultivating and differentiating stem cells obtained from differentiated exocrine glandular tissue of an organism. The use of the cells producing pancreatic hormone is also described.
摘要翻译：描述了通过培养和分化从生物体的分化的外分泌腺体组织获得的干细胞来产生产生胰腺激素的细胞的方法。还描述了产生胰腺激素的细胞的使用。

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