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1. 发明授权

US08298822B2 Method for the production of a cell composition containing epithelial cells 有权
标题翻译：用于生产含有上皮细胞的细胞组合物的方法
公开(公告)号：US08298822B2
公开(公告)日：2012-10-30
申请号：US11547678
申请日：2005-04-01
申请人： Charli Kruse , Gunter Fuhr , Thilo Wedel
发明人： Charli Kruse , Gunter Fuhr , Thilo Wedel
IPC分类号： C12N5/00 , C12N5/02 , C12N5/071 , A01N63/00
CPC分类号： C12N5/0677 , A61K35/24 , A61K35/26
摘要： Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.
摘要翻译：公开了形成上皮细胞的方法。所述方法包括以下步骤：从分化的外分泌腺组织聚集干细胞以获得有机体，并分化至少一部分有机体或由其生长的组织体以获得上皮细胞。还公开了一种培养装置，特别是用于形成差异上皮细胞的培养装置。

2. 发明申请

US20080193420A1 Method for the Production of a Cell Composition Containing Epithelial Cells 有权
标题翻译：含上皮细胞的细胞组合物的制备方法
公开(公告)号：US20080193420A1
公开(公告)日：2008-08-14
申请号：US11547678
申请日：2005-04-01
申请人： Charli Kruse , Gunter Fuhr , Thilo Wedel
发明人： Charli Kruse , Gunter Fuhr , Thilo Wedel
IPC分类号： A61K48/00 , C12N5/02 , C12M1/00
CPC分类号： C12N5/0677 , A61K35/24 , A61K35/26
摘要： Disclosed is a method for forming epithelial cells. Said method comprises the steps of aggregating stem cells from differentiated exocrine gland tissue to obtain an organoid body and differentiating at least one portion of the organoid body or a tissue body grown therefrom to obtain epithelial cells. Also disclosed is a cultivation device, particularly for forming differential epithelial cells.
摘要翻译：公开了形成上皮细胞的方法。所述方法包括以下步骤：从分化的外分泌腺组织聚集干细胞以获得有机体，并分化至少一部分有机体或由其生长的组织体以获得上皮细胞。还公开了一种培养装置，特别是用于形成差异上皮细胞的培养装置。

3. 发明申请

US20070248716A1 Method For the Production of a Biological Material Composition Having an Animal Origin 审中-公开
标题翻译：用于生产具有动物来源的生物材料组合物的方法
公开(公告)号：US20070248716A1
公开(公告)日：2007-10-25
申请号：US10599713
申请日：2005-03-04
申请人： Charli Kruse , Gunter Fuhr , Jurgen Rohwedel
发明人： Charli Kruse , Gunter Fuhr , Jurgen Rohwedel
IPC分类号： A23K1/00 , A23L1/00 , C12N5/02 , C12N5/06
CPC分类号： C12N5/0677
摘要： A method for producing a biological material composition of animal, non-human origin, especially as feed or food, is described. The method includes an aggregation of stem cells from differentiated exocrine glandular tissue of an animal organism to organoid bodies, and a preparation of the material composition from the organoid bodies.
摘要翻译：描述了用于生产动物，非人来源的生物材料组合物的方法，特别是作为饲料或食物。该方法包括将来自动物生物体的分化的外分泌腺体组织的干细胞与组织体的聚集，以及来自有机体的物质组合物的制备。

4. 发明申请

US20080193421A1 Multicellular Tissue and Organ Culture Systems 有权
标题翻译：多细胞组织和器官培养系统
公开(公告)号：US20080193421A1
公开(公告)日：2008-08-14
申请号：US11597317
申请日：2005-05-09
申请人： Charli Kruse , Gunter Fuhr
发明人： Charli Kruse , Gunter Fuhr
IPC分类号： A61K35/12 , C12N5/02 , A61P43/00 , A01N1/02
CPC分类号： A61L27/3886 , A61K35/12 , A61L27/3839 , C12N5/0062
摘要： The invention relates to methods for producing three-dimensional multicellular tissue and organ culture systems using multicellular mammalian cell aggregates, these systems themselves and their use for various medical purposes, in particular implantations.
摘要翻译：本发明涉及使用多细胞哺乳动物细胞聚集体制备三维多细胞组织和器官培养系统的方法，这些系统本身及其用于各种医疗目的，特别是植入物的用途。

5. 发明申请

US20080113400A1 Methods and Devices for Culturing Stem Cells 审中-公开
标题翻译：培养干细胞的方法和装置
公开(公告)号：US20080113400A1
公开(公告)日：2008-05-15
申请号：US11597309
申请日：2005-05-09
申请人： Charli Kruse , Gunter Fuhr
发明人： Charli Kruse , Gunter Fuhr
IPC分类号： C12N5/06 , C12Q1/02 , C12M1/00
CPC分类号： C12M23/48 , C12M21/10 , C12M23/50
摘要： Methods are described for the cultivation of stem cells (2, 3, 5), comprising the steps: Introduction of cell material comprising at least one stem cell (2, 3, 5) of a donor organism into a host egg (4) with a nutrient reservoir, and cultivation of the cell material in the host egg (4), a cell culture, and a cell culture device for carrying out the method.
摘要翻译：描述了用于培养干细胞（2,3,5）的方法，包括以下步骤：将包含供体生物体的至少一个干细胞（2,3,5）的细胞材料引入宿主卵（4）中，养殖池，宿主卵中细胞材料的培养（4），细胞培养物和用于实施该方法的细胞培养装置。

6. 发明授权

US09375514B2 Multicellular tissue and organ culture systems 有权
标题翻译：多细胞组织和器官培养系统
公开(公告)号：US09375514B2
公开(公告)日：2016-06-28
申请号：US11597317
申请日：2005-05-09
申请人： Charli Kruse , Gunter Fuhr
发明人： Charli Kruse , Gunter Fuhr
IPC分类号： C12N5/00 , A61L27/38 , A61K35/12
CPC分类号： A61L27/3886 , A61K35/12 , A61L27/3839 , C12N5/0062
摘要： The invention relates to methods for producing three-dimensional multicellular tissue and organ culture systems using multicellular mammalian cell aggregates, these systems themselves and their use for various medical purposes, in particular implantations.
摘要翻译：本发明涉及使用多细胞哺乳动物细胞聚集体制备三维多细胞组织和器官培养系统的方法，这些系统本身及其用于各种医疗目的，特别是植入物的用途。

7. 发明申请

US20080038806A1 Magnetic Manipulation of Biological Samples 有权
标题翻译：生物样品的磁操作
公开(公告)号：US20080038806A1
公开(公告)日：2008-02-14
申请号：US10591068
申请日：2005-02-25
申请人： Gunter Fuhr
发明人： Gunter Fuhr
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12M25/16
摘要： The invention relates to a cell carrier (10) for receiving a biological sample. Said carrier comprises a magnetic element (20) and a floor element (30) that forms a stable support (31) that can be displaced on a solid base surface in at least one direction. The invention also relates to a manipulation device for biological samples and to a method for manipulating biological samples.
摘要翻译：本发明涉及用于接收生物样品的细胞载体（10）。所述载体包括形成可在至少一个方向上在固体基底表面上移位的稳定支撑体（31）的磁性元件（20）和地板元件（30）。本发明还涉及用于生物样品的操作装置和用于操纵生物样品的方法。

8. 发明申请

US20060051735A1 Method and devices for transferring biological cells between a carrier and a probe 有权
标题翻译：用于在载体和探针之间转移生物细胞的方法和装置
公开(公告)号：US20060051735A1
公开(公告)日：2006-03-09
申请号：US10545829
申请日：2003-12-02
申请人： Gunter Fuhr , Heiko Zimmermann , Hagen Thielecke
发明人： Gunter Fuhr , Heiko Zimmermann , Hagen Thielecke
IPC分类号： C12M1/34 , C12Q1/00
CPC分类号： B01L3/021 , A61B10/0233 , A61B10/0283 , A61B2010/0208 , B01L2200/0647 , B01L2300/0838 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C12M33/02 , C12M35/00 , G01N1/286 , G01N15/12 , G01N15/14 , G01N2015/1486 , G01N2015/1493
摘要： A cell transfer method is described, in which transfer of at least one biological cell (21) describing a natural cell locomotion between a carrier (80) and a probe (10) is provided, and whereby positioning the probe (10) and the carrier (80) relative to one another in a transfer arrangement is provided, in which transfer of the cell (21) takes place due to the natural cell locomotion. Probes and cell manipulators for transferring biological cells are also described
摘要翻译：描述了一种细胞转移方法，其中提供描述载体（80）和探针（10）之间的天然细胞运动的至少一个生物细胞（21）的转移，由此将探针（10）和载体（80）在传送装置中相对于彼此设置，其中由于天然细胞运动而发生细胞（21）的转移。还描述了用于转移生物细胞的探针和细胞操纵器

9. 发明申请

US20060014134A1 Low-temperature storage of suspension samples in suspended sample chambers 失效
标题翻译：悬浮样品在悬浮样品室中的低温储存
公开(公告)号：US20060014134A1
公开(公告)日：2006-01-19
申请号：US10532017
申请日：2003-11-05
申请人： Gunter Fuhr , Heiko Zimmermann
发明人： Gunter Fuhr , Heiko Zimmermann
IPC分类号： A01N1/02 , F25C1/00
CPC分类号： A01N1/02 , A01N1/0257 , A01N1/0263 , A01N1/0268 , B01L3/505 , B01L3/545 , B01L2300/021 , B01L2300/024 , B01L2300/0838 , B01L2400/0406 , G01N1/42
摘要： A cryostorage device (100) is described that includes at least one data storage device (200) and at least one sample receptacle device (300) with at least one sample chamber (301, 302, etc.) for the uptake of a suspension sample, the at least one sample chamber (301, 302, etc.) being connected to the data storage device (200) and having a elongated, hollow shape that extends from an inlet end (320) over a predetermined length to an outlet end (330) and being attached to the at least one data storage device (200) in a flexible and movably hanging manner.
摘要翻译：描述了一种包括至少一个数据存储装置（200）和至少一个具有用于吸收悬浮液样品的至少一个样品室（301,302等）的样品容器装置（300）的冷冻装置（100），所述至少一个样品室（301,302等）连接到所述数据存储装置（200）并且具有从入口端（320）延伸超过预定长度的细长的中空形状到出口端（ 330）并且以柔性和可移动的悬挂方式附接到所述至少一个数据存储设备（200）。

10. 发明授权

US6149789A Process for manipulating microscopic, dielectric particles and a device therefor 失效
标题翻译：用于操作微观电介质颗粒的方法及其装置
公开(公告)号：US6149789A
公开(公告)日：2000-11-21
申请号：US508079
申请日：1995-07-27
申请人： Wolfgang Benecke , Bernd Wagner , Gunter Fuhr , Rolf Hagedorn , Thorsten Muller
发明人： Wolfgang Benecke , Bernd Wagner , Gunter Fuhr , Rolf Hagedorn , Thorsten Muller
IPC分类号： B01F13/00 , B01J19/00 , B03C5/00 , B03C5/02 , G01N27/447 , G01N27/26
CPC分类号： B01F13/0077 , B01F13/0076 , B01J19/0093 , B03C5/005 , B03C5/026 , B03C5/028 , B01F13/0059 , B01J2219/00853
摘要： Disclosed is a process for manipulating microscopic dielectric particles in which particles are exposed to an electric field. In disadvantaged processes the particles are exposed to inhomogeneous electric field and are polarized by them. The polarized particles are moved in the direction of higher field strength. These processes permit no reversal of the traveling direction of the particles. The particles are enriched at the electrode so that they cannot be retained in free space. In the invented process the particles are exposed to high-frequency field traveling in one or more prescribed direction, by which the particles are subject to a force which sets them in a motion that is strongly synchronous to the field. With the aid of a device for carrying out the invented process, the particles can be manipulated very flexibly. By means of linearly or circularly disposed electrodes, the particles can be moved on linear paths in any direction including through branched microstructures, they can be separated according to their dielectric properties and retained without contact. Applications are in the fields of biotechnology and molecular separation, focussing and microtransport technology.
摘要翻译：公开了一种操作微粒介质颗粒的方法，其中颗粒暴露于电场。在不利的过程中，颗粒暴露于不均匀的电场，并被它们极化。极化粒子沿高场强的方向移动。这些处理不允许反转颗粒的行进方向。颗粒在电极处富集，使得它们不能保留在自由空间中。在本发明的方法中，颗粒暴露于在一个或多个规定方向上行进的高频场，通过该方法，颗粒受到将其设置成与场强同步的运动的力。借助于用于实施本发明方法的装置，可以非常灵活地操纵颗粒。通过线性或圆形设置的电极，颗粒可以在包括通过分支微结构的任何方向上在线性路径上移动，它们可以根据其介电性质分离并保持而不接触。应用领域包括生物技术和分子分离，聚焦和微传输技术。

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