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1. 发明授权

US4467030A Determination of the thyroxine-binding index in serum 失效
标题翻译：血清中甲状腺素结合指数的测定
公开(公告)号：US4467030A
公开(公告)日：1984-08-21
申请号：US263948
申请日：1981-05-15
申请人： Gerd Kleinhammer , Gerlinde Deutsch , Hans-Ralf Linke , Fritz Stahler , Wolfgang Gruber
发明人： Gerd Kleinhammer , Gerlinde Deutsch , Hans-Ralf Linke , Fritz Stahler , Wolfgang Gruber
IPC分类号： G01N33/53 , C12Q1/28 , G01N33/78 , G01N33/54 , G01N33/58
CPC分类号： G01N33/78 , Y10S435/81 , Y10S435/975 , Y10S436/81
摘要： A method for the determination of the thyroxine-binding index in serum, which method comprises mixing the serum sample to be determined with a defined amount of thyroxine and a defined amount of a determinable enzyme covalently bound to thyroxine, contacting the resulting solution with anti-thyroxine antibodies present in the solid phase, separating the liquid phase from the solid phase, and measuring the activity of said determinable enzyme in one of the phases as a measure of the tyroxine-binding index in said serum sample.
摘要翻译：一种用于测定血清中甲状腺素结合指数的方法，该方法包括将待测定的血清样品与规定量的甲状腺素和限定量的与甲状腺素共价结合的可测定酶混合，存在于固相中的甲状腺素抗体，将液相与固相分离，并测定其中一相中所述可测定酶的活性，作为所述血清样品中酪氨酸结合指数的量度。

2. 发明授权

US6107055A Method and device for carrying out biochemical reactions 失效
标题翻译：进行生化反应的方法和装置
公开(公告)号：US6107055A
公开(公告)日：2000-08-22
申请号：US124985
申请日：1998-07-30
申请人： Hermann Bauer , Horst Menzler , Gerd Kleinhammer , Hans Schels , Peter Bendzko , Ute Fink , Bernd Maciej
发明人： Hermann Bauer , Horst Menzler , Gerd Kleinhammer , Hans Schels , Peter Bendzko , Ute Fink , Bernd Maciej
IPC分类号： C12M1/00 , C12M1/12 , C12M1/40 , C12P21/00 , C12P21/06 , C12P1/00
CPC分类号： C12M23/34 , C12M29/04 , C12M29/18
摘要： Method and apparatus for carrying out biochemical reactions in particular for cell-free polypeptide biosynthesis. A bioreactor has, within a housing, a reaction chamber containing a producing system as well as a supply chamber separated from the reaction chamber by means of a semi-permeable membrane and containing a liquid serving as supply medium for the producing system. The supply chamber is subdivided within the bioreactor into a first partial chamber and a second partial chamber which are each connected to the reaction chamber via a semi-permeable supply membrane and which are connected to each other by means of a connection conduit. During the biochemical reaction, liquid is circulated from the first partial chamber of the supply chamber through the reaction chamber and into the second partial chamber of the supply chamber in such a fashion that, during each passage of liquid out of the first partial chamber into the reaction chamber, liquid simultaneously passes from the reaction chamber into the second partial chamber and liquid flows out of the second partial chamber via the connection conduit back into the first partial chamber.
摘要翻译：用于进行生化反应的方法和装置，特别是用于无细胞的多肽生物合成。生物反应器在壳体内具有包含生产系统的反应室以及通过半透膜与反应室分离并且含有用作生产系统的供应介质的液体的供应室。供应室在生物反应器内被分为第一部分室和第二部分室，第一部分室和第二部分室通过半渗透供应膜连接到反应室，并通过连接导管相互连接。在生化反应期间，液体从供给室的第一部分室通过反应室循环，并进入供给室的第二部分室，使得在液体从第一部分室流出之前进入反应室，液体同时从反应室通入第二部分室，并且液体经由连接导管从第二部分室流出回到第一部分室中。

3. 发明授权

US4843001A Conjugate for enzyme immuno determinations 失效
标题翻译：用于酶免疫测定的缀合物
公开(公告)号：US4843001A
公开(公告)日：1989-06-27
申请号：US884431
申请日：1986-07-11
申请人： Harald Haug , Gerd Kleinhammer , Johann Mattersberger
发明人： Harald Haug , Gerd Kleinhammer , Johann Mattersberger
IPC分类号： G01N33/535
CPC分类号： G01N33/535 , Y10S435/81
摘要： This invention teaches a process for preparing a conjugate useful in enzyme immunoassays, and the conjugate produced by the process. The process involves oxidizing an enzyme containing a carbohydrate portion, such as peroxidase, with periodic acid or an alkali metal salt thereof in an aqueous medium followed by reduction with sodium borohydride. The oxidation and reduction step may be carried out either before or after the enzyme is coupled to the immunologically effective component of the conjugate.
摘要翻译：本发明教导了一种制备可用于酶免疫测定的缀合物的方法，以及通过该方法产生的缀合物。该方法包括在水性介质中将含有碳水化合物部分如过氧化物酶的酶与高碘酸或其碱金属盐氧化，然后用硼氢化钠还原。氧化和还原步骤可以在酶与缀合物的免疫有效成分偶联之前或之后进行。

4. 发明授权

US4732848A Process for the determination of an immunologically-bindable substance involving a Fab fragment 失效
标题翻译：测定涉及Fab片段的免疫结合物质的方法
公开(公告)号：US4732848A
公开(公告)日：1988-03-22
申请号：US767503
申请日：1985-08-20
申请人： Helmut Lenz , Gerd Kleinhammer
发明人： Helmut Lenz , Gerd Kleinhammer
IPC分类号： G01N33/543 , A61K39/395 , C12Q1/28 , C12Q1/34 , G01N33/535 , G01N33/563 , G01N33/573 , G01N33/53
CPC分类号： G01N33/535 , G01N33/563 , G01N33/573
摘要： The present invention provides a process for the determination of an immunologically-bindable substance by immunochemical methods in heterogeneous phase, wherein(a) a sample solution containing the substance to be determined is incubated with(b) a solid phase-bound antibody against the bindable substance to be determined(c) a conjugate of the bindable substance to be determined and a Fab fragment of an antibody directed against a determinable enzyme in a definite amount and(d) the enzyme which corresponds to the antibody contained in the conjugate (c),the phases are separated and the enzyme activity is measured in one of the two phases.The present invention also provides a reagent for the determination of an immunologically-bindable substance, wherein it contains a solid phase-bound antibody against the bindable substance to be determined, a conjugate of the bindable substance to be determined and a Fab fragment of an antibody directed against a determinable enzyme, a determinable enzyme which binds immunologically with the Fab fragment contained in the conjugate and buffer.
摘要翻译：本发明提供了一种通过免疫化学方法在非均相中测定免疫结合物质的方法，其中（a）将含有待测定物质的样品溶液与（b）针对可结合的固相结合抗体要确定的物质（c）待确定的可结合物质的缀合物和定向量的针对可测定酶的抗体的Fab片段和（d）对应于缀合物（c）中所含抗体的酶，分离各相，在两相中的一个相中测定酶活性。本发明还提供了用于测定免疫结合物质的试剂，其中其含有针对待测定的可结合物质的固相结合抗体，待测定的可结合物质的结合物和抗体的Fab片段针对可测定的酶，与包含在缀合物和缓冲液中的Fab片段免疫结合的可测定酶。

5. 发明授权

US4929543A Process for the determination of an antibody in human body fluids 失效
标题翻译：用于测定人体液中抗体的方法
公开(公告)号：US4929543A
公开(公告)日：1990-05-29
申请号：US192957
申请日：1988-05-12
申请人： Rosemarie Kientsch-Engel , Walter Worner , Gerd Kleinhammer
发明人： Rosemarie Kientsch-Engel , Walter Worner , Gerd Kleinhammer
IPC分类号： G01N33/531 , G01N33/543 , G01N33/563 , G01N33/68
CPC分类号： G01N33/6857 , G01N33/563 , G01N33/6854 , Y10S435/967 , Y10S435/974 , Y10S436/819 , Y10S436/826
摘要： The present invention provides a process for the determination of an antibody in human body fluids according to the immunoassay principle in which a sample containing the antibody to be determined is incubated with at least two different receptors R.sub.1 and R.sub.2, of which one receptor R.sub.1 carries an antigenic determinant specific for the antibody to be determined and one receptor R.sub.2 carries a label to form bound and unbound label, the part which contains the bound label is separated from the part which contains the unbound label and the label is measured in one of the two parts, wherein, for the control, instead of the sample, there is used a standard solution which contains a conjugate of a bindable non-human antibody or a Fab or F(ab').sub.2 fragment thereof which non-human component binds with a receptor R.sub.s which also binds to the antibody to be determined, and a human immunoglobulin or the Fc part thereof. The present invention also provides a control reagent for the determination of an antibody in human body fluids according to the immunoassay principle, containing a conjugate of an antibody bindable with the receptor R.sub.s specific for the antibody to be determined or a Fab or F(ab').sub.2 fragment thereof with a human immunoglobulin or the Fc part thereof, and said receptor.
摘要翻译：本发明提供了一种根据免疫测定原理测定人体液中的抗体的方法，其中将待测定的抗体的样品与至少两种不同的受体R1和R2一起孵育，其中一个受体R1携带待测抗体特异的抗原决定簇，一个受体R2携带标签形成结合和未结合的标签，含有结合标签的部分与含有未结合标签的部分分离，标记在两个其中，对于对照而不是样品，使用标准溶液，其含有可结合的非人抗体或Fab或F（ab'）2片段的缀合物，其非人类成分与还结合待测抗体的受体Rs，以及人免疫球蛋白或其Fc部分。本发明还提供了用于根据免疫测定原理测定人体液中的抗体的对照试剂，其含有可与待测抗体特异的受体Rs结合的抗体的缀合物或Fab或F（ab' ）2片段与人免疫球蛋白或其Fc部分和所述受体结合。

6. 发明授权

US4757016A Process for stabilizing the activity of peroxidase in solution 失效
标题翻译：稳定溶液中过氧化物酶活性的方法
公开(公告)号：US4757016A
公开(公告)日：1988-07-12
申请号：US835896
申请日：1986-03-04
申请人： Dagmar Klenner , Gerd Kleinhammer , Rolf Deeg
发明人： Dagmar Klenner , Gerd Kleinhammer , Rolf Deeg
IPC分类号： C12N9/96 , C12Q1/28 , G01N33/535 , C12N9/08
CPC分类号： C12N9/96 , C12Q1/28 , G01N33/535
摘要： The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, when the peroxidase is used as a conjugate with an immunologically effective substance, there is added, as activity stabilizer, aminopyrine in an amount of from 0.0005 to 2% by weight, referred to the solution.
摘要翻译：本发明提供了一种通过添加比活性稳定剂来稳定溶液中过氧化物酶活性的方法，其中以固体或溶解形式存在的酶，当过氧化物酶与免疫有效物质结合使用时，作为活性稳定剂，添加量为0.0005-2重量％的氨基比林，称为溶液。

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