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1. 发明公开

EP1857125A4 METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION 有权转让
标题翻译：方法制造板式再生软骨的
公开(公告)号：EP1857125A4
公开(公告)日：2010-09-22
申请号：EP06714869
申请日：2006-02-28
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： KATO YUKIO , TSUJI KOICHIRO , YAMANAKA KATSUYUKI
IPC分类号： C12N5/20 , A61L27/38
CPC分类号： A61L27/18 , A61L27/3817 , A61L27/3852 , A61L27/56 , A61L2430/06 , C12N5/0655 , C12N2501/15 , C12N2501/39 , C12N2533/40 , C08L67/04

2. 发明专利

AU2006219396A1 Method of fabricating sheet for cartilage tissue regeneration 未知
公开(公告)号：AU2006219396A1
公开(公告)日：2006-09-08
申请号：AU2006219396
申请日：2006-02-28
申请人： TWO CELLS CO LTD , G C DENTAL IND CORP
发明人： YAMANAKA KATSUYUKI , TSUJI KOICHIRO , KATO YUKIO
IPC分类号： A61L27/00 , A61K9/70 , A61K35/12 , A61K35/32 , A61K35/545 , A61K47/12 , A61K47/22 , A61K47/34 , A61K47/36 , A61L27/18 , A61L27/20 , A61L27/36 , A61L27/38 , A61L27/40 , A61L27/56 , A61L27/58 , A61P19/00 , C12M3/00 , C12N5/077
摘要： An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglyc-olic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.

3. 发明申请

WO2011111787A8 CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：含有中间干细胞的细胞制备方法及其生产方法
公开(公告)号：WO2011111787A8
公开(公告)日：2011-12-01
申请号：PCT/JP2011055683
申请日：2011-03-10
申请人： TWO CELLS CO LTD , TSUCHIYA TOSHIE , TSUJI KOICHIRO , KATO YUKIO , SHAO JIN CHANG , HARA MAIKO
发明人： TSUCHIYA TOSHIE , TSUJI KOICHIRO , KATO YUKIO , SHAO JIN CHANG , HARA MAIKO
IPC分类号： A61K35/12 , A61K35/28 , A61P37/06 , C12N5/0775
CPC分类号： C12N5/0663 , A61K35/12 , C12N5/0668 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15
摘要： Disclosed is a cell preparation, which contains mesenchymal stem cells that maintain immunosuppressive ability, and which is produced by serum-free or low-serum culture. Specifically disclosed is a method for producing a cell preparation containing mesenchymal stem cells, which is characterized by comprising: a proliferation step wherein mesenchymal stem cells are proliferated in a culture medium that contains FGF, PDGF, TGF-ß, HGF, EGF, at least one phospholipid and at least one fatty acid; and a screening step wherein the mesenchymal stem cells after the proliferation step are screened for mesenchymal stem cells in which the immunosuppressive ability is maintained or improved.
摘要翻译：公开了一种细胞制剂，其含有维持免疫抑制能力的间充质干细胞，其通过无血清或低血清培养产生。具体公开了一种含有间充质干细胞的细胞制剂的制造方法，其特征在于，包括：增殖步骤，其中间充质干细胞至少在含有FGF，PDGF，TGF-β，HGF，EGF的培养基中增殖一种磷脂和至少一种脂肪酸; 筛选步骤，其中对增殖步骤后的间充质干细胞进行筛选，其中维持或改善免疫抑制能力的间充质干细胞。

4. 发明申请

WO2004081174A3 MARKER FOR DETECTING MESENCHYMAL STEM CELL AND METHOD OF DISTINGUISHING MESENCHYMAL STEM CELL USING THE MARKER 审中-公开
标题翻译：用于检测中胚层干细胞的标记物和使用标记鉴别骨髓干细胞的方法
公开(公告)号：WO2004081174A3
公开(公告)日：2004-12-02
申请号：PCT/JP2004002457
申请日：2004-02-27
申请人： JAPAN SCIENCE & TECH AGENCY , KATO YUKIO , TWO CELLS CO LTD , TSUJI KOICHIRO , KOIKE CHIKA
发明人： KATO YUKIO , TSUJI KOICHIRO , KOIKE CHIKA
IPC分类号： G01N33/53 , C07K14/47 , C07K16/18 , C12N20060101 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/113 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N37/00 , C12N15/11
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： It is intended to provide a marker for detecting, separating and distinguishing a mesenchymal stem cell and a method of detecting, separating and distinguishing a mesenchymal stem cell by using this marker. A gene shown in Sequence Listing is expressed specifically in a mesenchymal stem cell. This gene is employed as a marker for detecting a mesenchymal stem cell. It is further intended to provide a probe for detecting the mesenchymal stem cell marker gene and a PCR primer for amplifying the gene in a sample cell in detecting the mesenchymal stem cell gene marker. It is furthermore intended to provide a polypeptide marker for detecting a mesenchymal stem cell comprising a polypeptide in which the above-described mesenchymal stem cell marker gene is expressed, an antibody binding specifically to the polypeptide marker for detecting the polypeptide marker, a probe for detecting the mesenchymal stem cell marker gene, and a method of distinguishing and separating a mesenchymal stem cell by using the antibody binding specifically to the polypeptide marker.
摘要翻译：旨在提供用于检测，分离和鉴别间充质干细胞的标记物和通过使用该标记物检测，分离和区分间充质干细胞的方法。序列表中所示的基因特异性地在间充质干细胞中表达。该基因用作检测间充质干细胞的标记物。还旨在提供用于检测间充质干细胞标记基因的探针和用于在检测间充质干细胞基因标记物中扩增样品细胞中的基因的PCR引物。此外，还提供了用于检测间充质干细胞的多肽标记物，其包含其中表达上述间充质干细胞标记基因的多肽，特异性结合用于检测多肽标记的多肽标记物的抗体，检测用探针间充质干细胞标记基因，以及通过使用与多肽标记特异性结合的抗体来区分和分离间充质干细胞的方法。

5. 发明专利

JP2012143158A Inducible mesenchymal stem cell and method for preparing the same 审中-公开
标题翻译：可诱导的中胚层干细胞及其制备方法
公开(公告)号：JP2012143158A
公开(公告)日：2012-08-02
申请号：JP2009115378
申请日：2009-05-12
申请人： Hiroshima Univ , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル
发明人： KATO YUKIO , KAWAMOTO TAKESHI , NISHIMURA MASAHIRO , TSUJI KOICHIRO
IPC分类号： C12N5/10 , C12N15/09 , C12Q1/68
CPC分类号： C12N5/0662 , C12N2510/00
摘要： PROBLEM TO BE SOLVED: To provide a cell having self-renewal potency and differentiation potency similar to those of a mesenchymal stem cell (MSC) without utilizing a human embryo or embryonic stem cell (ES cell).SOLUTION: There are provided a method for preparing an inducible MSC according to a method including a step of introducing at least one of genes having a specific base sequence into a cell; a kit for preparation; a method for detecting the inducible MSC by detecting expression of the gene; a kit for detection; a microarray for the detection; and a method for screening a factor controlling the differentiation potency of the MSC.
摘要翻译：要解决的问题：提供具有与间充质干细胞（MSC）类似的自我更新效力和分化能力的细胞，而不利用人胚胎或胚胎干细胞（ES细胞）。解决方案：提供了一种根据包括将至少一个具有特定碱基序列的基因导入细胞的方法制备可诱导MSC的方法，一套准备工具; 通过检测该基因的表达来检测诱导型MSC的方法; 检测用试剂盒用于检测的微阵列; 以及筛选控制MSC的分化潜能的因素的方法。版权所有（C）2012，JPO＆INPIT

6. 发明专利

JP2009011167A Method for screening substance controlling hypoxic response, and pharmaceutical composition controlling hypoxic response 审中-公开
标题翻译：筛选物质控制高血压反应的方法和控制高血压反应的药物组合物
公开(公告)号：JP2009011167A
公开(公告)日：2009-01-22
申请号：JP2007173127
申请日：2007-06-29
申请人： Hiroshima Univ , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル
发明人： KATO YUKIO , KAWAMOTO TAKESHI , NAKAJIMA AYUMI , TSUJI KOICHIRO
IPC分类号： C12Q1/68 , A61K31/711 , A61K45/00 , A61K48/00 , A61P9/00 , A61P9/08 , A61P9/10 , A61P35/00 , C12N15/09 , C12Q1/02 , G01N33/15 , G01N33/50 , G01N33/68
摘要： PROBLEM TO BE SOLVED: To analyze a hypoxic response associated with DEC1 in diseases related to a hypoxic state in, e.g. cancer or ischemic diseases, and to provide a pharmaceutical composition for prophylaxis or therapy of the diseases.
SOLUTION: A substance having effects on the therapy or the prophylaxis of the diseases related to the hypoxic response can readily be screened by a method for screening a DEC1-mediating substance or a method for screening a DEC1-non-mediating substance. Furthermore, the therapy or the prophylaxis of the cancer or the ischemic diseases can be carried out by the pharmaceutical composition, comprising the screened substance or the substance promoting or inhibiting functions of the DEC1.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：分析与缺氧状态相关的疾病中与DEC1相关的缺氧反应。癌症或缺血性疾病，并提供用于预防或治疗疾病的药物组合物。解决方案：通过用于筛选DEC1介导物质的方法或用于筛选DEC1非介导物质的方法，可以容易地筛选对治疗或预防与缺氧反应相关的疾病有影响的物质。此外，可以通过包含筛选物质或促进或抑制DEC1功能的物质的药物组合物来进行癌症或缺血性疾病的治疗或预防。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2006000059A Method for promoting proliferation and differentiation of animal cell by using extracellular substrate 审中-公开
标题翻译：通过使用细胞基质促进动物细胞增殖和分化的方法
公开(公告)号：JP2006000059A
公开(公告)日：2006-01-05
申请号：JP2004180338
申请日：2004-06-17
申请人： Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル
发明人： KATO YUKIO , HARA TETSUAKI , TSUJI KOICHIRO
IPC分类号： C12M1/22 , C12M1/24 , C12M3/00 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N5/0789 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a culturing method for obtaining extremely more animal cells compared to a conventional method by promoting proliferation and differentiation-inducing of the cultured animal cells in the proliferation culture and differentiation-inducing culture of stem cells or the like of various kinds of animals while effectively keeping the differentiation abilities of the animal stem cells or the like under proliferation. SOLUTION: The proliferation of the cultured animal cells of various kinds of the animal cells is promoted by culturing the animal cells in the presence of an extracellular substrate derived from a mesenchymal cell, an epithelial cell, a cartilaginous precursor cell or a fibroblast. Further, the differentiation ability of the cultured animal stem cells or the like is effectively maintained under the proliferation, and the differentiation induction is promoted to enable the excellent differentiation-inducing culture of the animal stem cells to be carried out. Preferably, even serum in a low concentration can be effectively proliferated and the culture by using the human serum can be carried out by using the method of culturing the animal cells and adding various kinds of additives to a medium. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：为了提供与传统方法相比获得极其多的动物细胞的培养方法，通过促进培养的动物细胞在增殖培养物和干细胞分化诱导培养中的增殖和分化诱导像各种动物一样有效地保持动物干细胞等在增殖下的分化能力。解决方案：通过在源自间充质细胞，上皮细胞，软骨前体细胞或成纤维细胞的细胞外底物的存在下培养动物细胞来促进各种动物细胞的培养动物细胞的增殖。此外，培养的动物干细胞等的分化能力在增殖下有效地保持，促进分化诱导，使得能够进行优异的分化诱导的动物干细胞的培养。优选地，即使是低浓度的血清也可以有效地增殖，并且可以通过使用培养动物细胞并向培养基中添加各种添加剂的方法来进行使用人血清的培养。版权所有（C）2006，JPO＆NCIPI

8. 发明专利

JP2007037426A Serum-free culture medium for culturing animal stem cell 有权
标题翻译：用于培养动物干细胞的无血清培养基
公开(公告)号：JP2007037426A
公开(公告)日：2007-02-15
申请号：JP2005223242
申请日：2005-08-01
申请人： Hiroki Futagawa , Two Cells Co Ltd , 浩樹二川 , 株式会社ツーセル
发明人： FUTAGAWA HIROKI , NISHIMURA MASAHIRO , KATO YUKIO , TSUJI KOICHIRO
IPC分类号： C12N5/02 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N5/0789
摘要： PROBLEM TO BE SOLVED: To provide a serum-free culture medium for culturing animal stem cells, which is used for culturing and proliferating the animal stem cells used for anagenetic medical treatments and the like and gives sufficient proliferation equal to those in cases of culture media using serums, and to provide a method for culturing animal stem cells in the serum-free culture medium. SOLUTION: This serum-free culture medium for culturing the animal stem cells is characterized by adding an anti-microbial peptide having a specific amino acid sequence to a serum-free basal culture medium for culturing animal cells. The anti-microbial peptide can be prepared by synthesis. The serum-free culture medium for culturing the animal stem cells can prevent various problems caused when serums are used, can exhibit sufficient proliferation effects equal to those in cases of culture media using the serums, and can provide an excellent culture medium for culturing and proliferating animal stem cells, such as mesenchymal stem cells, used for anagenetic medical treatments. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供用于培养动物干细胞的无血清培养基，其用于培养和增殖用于成像医学治疗等的动物干细胞，并产生与实例中相同的增殖的培养基，并提供在无血清培养基中培养动物干细胞的方法。解决方案：用于培养动物干细胞的无血清培养基的特征在于向无血清的基础培养基中加入具有特定氨基酸序列的抗微生物肽以培养动物细胞。抗微生物肽可以通过合成来制备。用于培养动物干细胞的无血清培养基可以防止使用血清时引起的各种问题，可以发挥与使用血清的培养基相同的增殖效果，并且可以提供用于培养和增殖的优良培养基动物干细胞，如间充质干细胞，用于成像医学治疗。版权所有（C）2007，JPO＆INPIT

9. 发明专利

JP2005027579A Method for identifying/isolating mesenchymal stem cell by using fraction marker 有权
$Method for identifying/isolating mesenchymal stem cell by using fraction marker$
标题翻译：使用分类标记识别/分离中胚层干细胞的方法
公开(公告)号：JP2005027579A
公开(公告)日：2005-02-03
申请号：JP2003271649
申请日：2003-07-07
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , HARA MAIKO
IPC分类号： G01N33/48 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/566 , G01N33/577 , G01N33/68 , G01N37/00 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a method for effectively identifying and isolating a mesenchymal stem cell and a fibroblast the distinction of which is difficult because of resembled shapes by using a gene marker and/or a protein marker. SOLUTION: The mesenchymal stem cell and the fibroblast are identified/isolated by using a gene of a vitamin D receptor or a protein thereof, or a vitamin D receptor-associated gene such as osteocalcin and osteopontin, or a protein thereof as a fraction marker of the mesenchymal stem cell and the fibroblast, and detecting the gene and the expression of the protein in a subject cell. The identification/isolation of the mesenchymal stem cell is effectively carried out by detecting the expression of an activated gene because the expression of the target gene of the vitamin D receptor is activated in the mesenchymal stem cell by the addition of a material for activating the expression of the gene such as 1,25-dihydroxyvitamine D. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种有效鉴定和分离间充质干细胞和成纤维细胞的方法，其区别由于使用基因标记和/或蛋白质标记的类似形状而难以进行。解决方案：通过使用维生素D受体或其蛋白质的基因或维生素D受体相关基因如骨钙蛋白和骨桥蛋白或其蛋白质来鉴定/分离间充质干细胞和成纤维细胞作为间充质干细胞和成纤维细胞的分数标记，并检测受试细胞中蛋白质的基因和表达。通过检测活化基因的表达来有效地进行间充质干细胞的鉴定/分离，因为维生素D受体的靶基因的表达通过添加用于激活表达的材料在间充质干细胞中活化的基因如1,25-二羟基维生素D.版权所有（C）2005，JPO＆NCIPI

10. 发明专利

JP2004290189A Marker for detecting mesenchymal stem cell detecting marker and method for discriminating the mesenchymal stem cell by using the marker 审中-公开
标题翻译：检测MESENCHYMAL STEM CELL检测标记的标记和使用标记鉴别MESENCHYMAL STEM细胞的方法
公开(公告)号：JP2004290189A
公开(公告)日：2004-10-21
申请号：JP2004067037
申请日：2004-03-10
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , KOIKE CHIKA
IPC分类号： G01N33/53 , C07K14/47 , C07K16/18 , C12N20060101 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/113 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N37/00
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a marker for detecting, separating, and/or discriminating a mesenchymal stem cell, and to provide a method for detecting, separating, and/or discriminating the mesenchymal stem cell, by using the marker. SOLUTION: A gene given in sequence table is specifically expressed in the mesenchymal stem cell. The method for discriminating the mesenchymal stem cell comprises using the gene as the marker for detecting the mesenchymal stem cell. Further, a probe for detecting a mesenchymal stem cell marker gene, a primer for polymerase chain reaction used for amplifying the gene of a specimen cell in a case of detecting the mesenchymal stem cell gene marker, a polypeptide marker for detecting the mesenchymal stem cell comprising a polypeptide which the mesenchymal stem cell marker gene expresses, an antibody used for detecting the peptide marker and specifically combining with the polypeptide marker, and the method for discriminating and separating the mesenchymal stem cell which comprises using the probe for detecting the mesenchymal stem cell marker gene and the antibody specifically combining with the polypeptide marker are provided, respectively. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于检测，分离和/或区分间充质干细胞的标记物，并提供通过使用标记物来检测，分离和/或鉴别间充质干细胞的方法。解决方案：序列表中给出的基因在间充质干细胞中特异性表达。用于鉴别间充质干细胞的方法包括使用该基因作为检测间充质干细胞的标志物。此外，在检测间充质干细胞基因标记的情况下，用于检测间充质干细胞标记基因的探针，用于扩增样本细胞基因的聚合酶链反应的引物，用于检测间充质干细胞的多肽标记物，包括间充质干细胞标记基因表达的多肽，用于检测肽标记并与多肽标记特异性结合的抗体，以及鉴别和分离间充质干细胞的方法，其包括使用该探针检测间充质干细胞标记分别提供与多肽标记物特异性结合的抗体基因。版权所有（C）2005，JPO＆NCIPI

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