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1. 发明专利

JP2005027579A Method for identifying/isolating mesenchymal stem cell by using fraction marker 有权
$Method for identifying/isolating mesenchymal stem cell by using fraction marker$
标题翻译：使用分类标记识别/分离中胚层干细胞的方法
公开(公告)号：JP2005027579A
公开(公告)日：2005-02-03
申请号：JP2003271649
申请日：2003-07-07
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , HARA MAIKO
IPC分类号： G01N33/48 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/566 , G01N33/577 , G01N33/68 , G01N37/00 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a method for effectively identifying and isolating a mesenchymal stem cell and a fibroblast the distinction of which is difficult because of resembled shapes by using a gene marker and/or a protein marker. SOLUTION: The mesenchymal stem cell and the fibroblast are identified/isolated by using a gene of a vitamin D receptor or a protein thereof, or a vitamin D receptor-associated gene such as osteocalcin and osteopontin, or a protein thereof as a fraction marker of the mesenchymal stem cell and the fibroblast, and detecting the gene and the expression of the protein in a subject cell. The identification/isolation of the mesenchymal stem cell is effectively carried out by detecting the expression of an activated gene because the expression of the target gene of the vitamin D receptor is activated in the mesenchymal stem cell by the addition of a material for activating the expression of the gene such as 1,25-dihydroxyvitamine D. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种有效鉴定和分离间充质干细胞和成纤维细胞的方法，其区别由于使用基因标记和/或蛋白质标记的类似形状而难以进行。解决方案：通过使用维生素D受体或其蛋白质的基因或维生素D受体相关基因如骨钙蛋白和骨桥蛋白或其蛋白质来鉴定/分离间充质干细胞和成纤维细胞作为间充质干细胞和成纤维细胞的分数标记，并检测受试细胞中蛋白质的基因和表达。通过检测活化基因的表达来有效地进行间充质干细胞的鉴定/分离，因为维生素D受体的靶基因的表达通过添加用于激活表达的材料在间充质干细胞中活化的基因如1,25-二羟基维生素D.版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004290189A Marker for detecting mesenchymal stem cell detecting marker and method for discriminating the mesenchymal stem cell by using the marker 审中-公开
标题翻译：检测MESENCHYMAL STEM CELL检测标记的标记和使用标记鉴别MESENCHYMAL STEM细胞的方法
公开(公告)号：JP2004290189A
公开(公告)日：2004-10-21
申请号：JP2004067037
申请日：2004-03-10
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , KOIKE CHIKA
IPC分类号： G01N33/53 , C07K14/47 , C07K16/18 , C12N20060101 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/113 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N37/00
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a marker for detecting, separating, and/or discriminating a mesenchymal stem cell, and to provide a method for detecting, separating, and/or discriminating the mesenchymal stem cell, by using the marker. SOLUTION: A gene given in sequence table is specifically expressed in the mesenchymal stem cell. The method for discriminating the mesenchymal stem cell comprises using the gene as the marker for detecting the mesenchymal stem cell. Further, a probe for detecting a mesenchymal stem cell marker gene, a primer for polymerase chain reaction used for amplifying the gene of a specimen cell in a case of detecting the mesenchymal stem cell gene marker, a polypeptide marker for detecting the mesenchymal stem cell comprising a polypeptide which the mesenchymal stem cell marker gene expresses, an antibody used for detecting the peptide marker and specifically combining with the polypeptide marker, and the method for discriminating and separating the mesenchymal stem cell which comprises using the probe for detecting the mesenchymal stem cell marker gene and the antibody specifically combining with the polypeptide marker are provided, respectively. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于检测，分离和/或区分间充质干细胞的标记物，并提供通过使用标记物来检测，分离和/或鉴别间充质干细胞的方法。解决方案：序列表中给出的基因在间充质干细胞中特异性表达。用于鉴别间充质干细胞的方法包括使用该基因作为检测间充质干细胞的标志物。此外，在检测间充质干细胞基因标记的情况下，用于检测间充质干细胞标记基因的探针，用于扩增样本细胞基因的聚合酶链反应的引物，用于检测间充质干细胞的多肽标记物，包括间充质干细胞标记基因表达的多肽，用于检测肽标记并与多肽标记特异性结合的抗体，以及鉴别和分离间充质干细胞的方法，其包括使用该探针检测间充质干细胞标记分别提供与多肽标记物特异性结合的抗体基因。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2007314432A New antimicrobial peptide and serum-free medium containing the antimicrobial peptide as active ingredient 审中-公开
标题翻译：含抗微生物肽作为活性成分的新型抗微生物肽和无血清培养基
公开(公告)号：JP2007314432A
公开(公告)日：2007-12-06
申请号：JP2006142505
申请日：2006-05-23
申请人： Hiroshima Univ , Japan Science & Technology Agency , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： NIKAWA HIROKI , NISHIMURA MASAHIRO , TSUJI KOICHIRO , KAWABATA RYOKO , HIROMOTO NOBUE
IPC分类号： C07K14/47 , A61K38/00 , A61P31/04 , A61P31/10 , C12N5/10
摘要： PROBLEM TO BE SOLVED: To obtain a new peptide that performs high antibacterial effect, cell growth promoting action and bone differentiation inducing action so as to be a substitute for an autologous serum or bovine fetal calf serum and is contained as an active ingredient in a serum-free medium. SOLUTION: The antimicrobial peptide comprises a specific amino acid sequence. The antifungal agent comprises the peptide. The composition having sterilization and/or growth inhibitory action contains an effective amount of the peptide as an active ingredient. The medical device and apparatus contain the peptide and have antimicrobial property. The method for sterilization and/or growth inhibition of fungi comprises administration of the peptide. The cell growth promoting factor comprises the antimicrobial peptide. The bone differentiation inducing factor comprises the antimicrobial peptide. The serum-free medium comprises the antimicrobial peptide. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：为了获得具有高抗菌作用，细胞生长促进作用和骨分化诱导作用的新型肽，以替代自体血清或牛胎牛血清，并且作为有效成分在无血清培养基中。解决方案：抗微生物肽包含特定的氨基酸序列。抗真菌剂包含肽。具有灭菌和/或生长抑制作用的组合物含有有效量的肽作为活性成分。医疗装置和装置含有肽并具有抗菌性。灭菌和/或生长抑制真菌的方法包括施用肽。细胞生长促进因子包括抗微生物肽。骨分化诱导因子包括抗微生物肽。无血清培养基包含抗微生物肽。版权所有（C）2008，JPO＆INPIT

4. 发明专利

JP2005281225A New basic antibacterial peptide and use thereof 审中-公开
标题翻译：新型基础抗菌肽及其用途
公开(公告)号：JP2005281225A
公开(公告)日：2005-10-13
申请号：JP2004098896
申请日：2004-03-30
申请人： Japan Science & Technology Agency , 独立行政法人科学技術振興機構
发明人： FUTAGAWA HIROKI , HAMADA TAIZO , NISHIMURA MASAHIRO , TSUJI KOICHIRO
IPC分类号： A61K8/00 , A61K8/64 , A61K8/72 , A61K38/00 , A61L27/00 , A61P1/02 , A61P31/04 , A61Q11/00 , C07K7/08 , C12N5/0775 , A61K7/00 , A61K7/16 , C12N5/06
CPC分类号： C07K7/08 , A61K38/00 , C12N5/0663 , C12N2501/998
摘要： PROBLEM TO BE SOLVED: To provide a new antibacterial peptide having extremely small side or inhibiting effect on living bodies and useful activity, and to provide a method for use of the peptide as an antibacterial agent or cell proliferation promoter. SOLUTION: The antibacterial peptide is a derivative in which one or several amino acids in the amino acid sequence are lost, replaced or added. The new antibacterial peptide comprising such specified amino acid sequence has a wide spectrum and strong antibacterial activity to oral microorganisms, particularly having strong bactericidal action on Streptococcus mutans being a carious bacillus, and additionally has an activity of promoting the proliferation of cells such as a mesenchymal stem cell or fibroblast. Further, this invention provides the use of the peptide as an antibacterial agent or cell proliferation promotor. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种对生物体具有极小侧面或抑制作用的新的抗菌肽和有用的活性，并提供使用该肽作为抗菌剂或细胞增殖促进剂的方法。解决方案：抗菌肽是氨基酸序列中的一个或几个氨基酸丢失，替换或添加的衍生物。包含这种特定氨基酸序列的新抗菌肽对口腔微生物具有广谱和强抗菌活性，特别是对变形链球菌为龋芽孢杆菌具有强烈的杀菌作用，另外具有促进细胞增殖的活性，例如间充质干细胞或成纤维细胞。此外，本发明提供了肽作为抗菌剂或细胞增殖促进剂的用途。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2005154338A Cell proliferating agent containing basic antibacterial peptide as active ingredient 有权
标题翻译：含有基础抗菌肽作为活性成分的细胞增殖剂
公开(公告)号：JP2005154338A
公开(公告)日：2005-06-16
申请号：JP2003395008
申请日：2003-11-26
申请人： Japan Science & Technology Agency , 独立行政法人科学技術振興機構
发明人： FUTAGAWA HIROKI , HAMADA TAIZO , AOKI YOSHIE , NISHIMURA MASAHIRO , TSUJI KOICHIRO
IPC分类号： A61K35/32 , A61K38/00 , A61P1/02 , A61P31/04 , A61P43/00 , C07K7/08 , C07K14/47 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a cell proliferating agent capable of efficiently proliferating various kinds of cells such as a mesenchymal stem cell in vitro or the like; and to provide a method for proliferating the cells by using the cell proliferating agent, very important also in a view point of prevention of postoperative infection in tissue transplantation in actual clinic application. SOLUTION: The mesenchymal stem cell is proliferated in the presence of a designed antibacterial peptide such as the antibacterial peptide derived from a natural product such as a signal peptide of a mitochondrion, histatin-5, and bovine or human lactoferrcin. A cell growth factor such as a basic fibroblast growth factor can coexist therewith. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供能够在体外等有效增殖各种细胞如间充质干细胞的细胞增殖剂; 并且通过使用细胞增殖剂提供增殖细胞的方法，在实际临床应用中在预防组织移植术后感染的观点也非常重要。在设计的抗菌肽存在下增殖间充质干细胞，例如衍生自天然产物如线粒体信号肽，组氨素-5和牛或人乳铁蛋白的抗菌肽。细胞生长因子如碱性成纤维细胞生长因子可与其共存。版权所有（C）2005，JPO＆NCIPI

6. 发明专利

JP2006000059A Method for promoting proliferation and differentiation of animal cell by using extracellular substrate 审中-公开
标题翻译：通过使用细胞基质促进动物细胞增殖和分化的方法
公开(公告)号：JP2006000059A
公开(公告)日：2006-01-05
申请号：JP2004180338
申请日：2004-06-17
申请人： Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル
发明人： KATO YUKIO , HARA TETSUAKI , TSUJI KOICHIRO
IPC分类号： C12M1/22 , C12M1/24 , C12M3/00 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N5/0789 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a culturing method for obtaining extremely more animal cells compared to a conventional method by promoting proliferation and differentiation-inducing of the cultured animal cells in the proliferation culture and differentiation-inducing culture of stem cells or the like of various kinds of animals while effectively keeping the differentiation abilities of the animal stem cells or the like under proliferation. SOLUTION: The proliferation of the cultured animal cells of various kinds of the animal cells is promoted by culturing the animal cells in the presence of an extracellular substrate derived from a mesenchymal cell, an epithelial cell, a cartilaginous precursor cell or a fibroblast. Further, the differentiation ability of the cultured animal stem cells or the like is effectively maintained under the proliferation, and the differentiation induction is promoted to enable the excellent differentiation-inducing culture of the animal stem cells to be carried out. Preferably, even serum in a low concentration can be effectively proliferated and the culture by using the human serum can be carried out by using the method of culturing the animal cells and adding various kinds of additives to a medium. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：为了提供与传统方法相比获得极其多的动物细胞的培养方法，通过促进培养的动物细胞在增殖培养物和干细胞分化诱导培养中的增殖和分化诱导像各种动物一样有效地保持动物干细胞等在增殖下的分化能力。解决方案：通过在源自间充质细胞，上皮细胞，软骨前体细胞或成纤维细胞的细胞外底物的存在下培养动物细胞来促进各种动物细胞的培养动物细胞的增殖。此外，培养的动物干细胞等的分化能力在增殖下有效地保持，促进分化诱导，使得能够进行优异的分化诱导的动物干细胞的培养。优选地，即使是低浓度的血清也可以有效地增殖，并且可以通过使用培养动物细胞并向培养基中添加各种添加剂的方法来进行使用人血清的培养。版权所有（C）2006，JPO＆NCIPI

7. 发明专利

JP2006204200A Agent for accelerating ossification and/or chondrification of mesenchymal stem cell and method for accelerating ossification and/or chondrification 有权
标题翻译：用于加速卵母细胞的移植和/或重建的代理和用于加速移植和/或修复的方法
公开(公告)号：JP2006204200A
公开(公告)日：2006-08-10
申请号：JP2005021336
申请日：2005-01-28
申请人： Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル
发明人： KATO YUKIO , NISHIMURA MASAHIRO , NISHIMURA HARUKI , TSUJI KOICHIRO , SEKIYA KENSUKE
IPC分类号： A61K35/12 , A61K36/48 , A61K38/00 , A61L27/00 , A61P19/00 , A61P19/02 , A61P19/08 , A61P19/10 , A61P29/00 , C12N5/07 , C12N5/074 , C12N5/0775
摘要： PROBLEM TO BE SOLVED: To provide a method for accelerating regeneration of a bone or cartilage, and to provide a medicament for the same. SOLUTION: This medicament for accelerating ossification and/or chondrification of a mesenchymal stem cell contains lectin as an active ingredient. The medicament is preferably added to a culture medium of the mesenchymal stem cell. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种促进骨或软骨再生的方法，并提供其用途。解决方案：用于促进间充质干细胞的骨化和/或软骨化的药物含有凝集素作为活性成分。药物优选加入到间充质干细胞的培养基中。版权所有（C）2006，JPO＆NCIPI

8. 发明专利

JP2011067175A Method for culturing mesenchymal stem cell 有权
标题翻译：培养细胞干细胞的方法
公开(公告)号：JP2011067175A
公开(公告)日：2011-04-07
申请号：JP2009222981
申请日：2009-09-28
申请人： Gc Corp , Two Cells Co Ltd , 株式会社ジーシー , 株式会社ツーセル
发明人： SAKAI YUUDAI , YAMANAKA KATSUYUKI , TAKEDA MIKA , OKURA TOMOHISA , TSUJI KOICHIRO
IPC分类号： C12N5/07
CPC分类号： C12N5/0663 , C12N2500/50
摘要： PROBLEM TO BE SOLVED: To provide a method for culturing mesenchymal stem cells, which efficiently selects and proliferates mesenchymal stem cells without requiring a separator of exclusive use and complicated separation operation. SOLUTION: One or more of bone marrow liquid, cord blood, peripheral blood, synovial membrane and amnion are seeded in a liquid medium that is filled in a container and comprises water having a specific gravity of 1.06-1.10 g/mL at 37°C as a main component and a culture is carried out on the ceiling surface of the container at 37±2°C to culture the mesenchymal stem cells. Preferably the specific gravity of the liquid medium is adjusted by using at least one selected from silica fine powder coated with a polyvinylpyrrolidone, a water-soluble copolymer of sucrose and epichlorohydrin and a water-soluble compound containing a triiodo aromatic ring. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供一种培养间充质干细胞的方法，其能够有效地选择和增殖间充质干细胞，而不需要专用分离器和复杂的分离操作。解决方案：将一种或多种骨髓液，脐带血，外周血，滑膜和羊膜接种在装在容器中的液体培养基中，并包含比重为1.06-1.10g / mL的水 37℃作为主要成分，并在37±2℃的容器的顶棚表面进行培养，以培养间充质干细胞。优选地，通过使用选自涂覆有聚乙烯吡咯烷酮的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和含有三碘芳环的水溶性化合物中的至少一种来调节液体介质的比重。版权所有（C）2011，JPO＆INPIT

9. 发明专利

JP2012143158A Inducible mesenchymal stem cell and method for preparing the same 审中-公开
标题翻译：可诱导的中胚层干细胞及其制备方法
公开(公告)号：JP2012143158A
公开(公告)日：2012-08-02
申请号：JP2009115378
申请日：2009-05-12
申请人： Hiroshima Univ , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル
发明人： KATO YUKIO , KAWAMOTO TAKESHI , NISHIMURA MASAHIRO , TSUJI KOICHIRO
IPC分类号： C12N5/10 , C12N15/09 , C12Q1/68
CPC分类号： C12N5/0662 , C12N2510/00
摘要： PROBLEM TO BE SOLVED: To provide a cell having self-renewal potency and differentiation potency similar to those of a mesenchymal stem cell (MSC) without utilizing a human embryo or embryonic stem cell (ES cell).SOLUTION: There are provided a method for preparing an inducible MSC according to a method including a step of introducing at least one of genes having a specific base sequence into a cell; a kit for preparation; a method for detecting the inducible MSC by detecting expression of the gene; a kit for detection; a microarray for the detection; and a method for screening a factor controlling the differentiation potency of the MSC.
摘要翻译：要解决的问题：提供具有与间充质干细胞（MSC）类似的自我更新效力和分化能力的细胞，而不利用人胚胎或胚胎干细胞（ES细胞）。解决方案：提供了一种根据包括将至少一个具有特定碱基序列的基因导入细胞的方法制备可诱导MSC的方法，一套准备工具; 通过检测该基因的表达来检测诱导型MSC的方法; 检测用试剂盒用于检测的微阵列; 以及筛选控制MSC的分化潜能的因素的方法。版权所有（C）2012，JPO＆INPIT

10. 发明专利

JP2009011167A Method for screening substance controlling hypoxic response, and pharmaceutical composition controlling hypoxic response 审中-公开
标题翻译：筛选物质控制高血压反应的方法和控制高血压反应的药物组合物
公开(公告)号：JP2009011167A
公开(公告)日：2009-01-22
申请号：JP2007173127
申请日：2007-06-29
申请人： Hiroshima Univ , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル
发明人： KATO YUKIO , KAWAMOTO TAKESHI , NAKAJIMA AYUMI , TSUJI KOICHIRO
IPC分类号： C12Q1/68 , A61K31/711 , A61K45/00 , A61K48/00 , A61P9/00 , A61P9/08 , A61P9/10 , A61P35/00 , C12N15/09 , C12Q1/02 , G01N33/15 , G01N33/50 , G01N33/68
摘要： PROBLEM TO BE SOLVED: To analyze a hypoxic response associated with DEC1 in diseases related to a hypoxic state in, e.g. cancer or ischemic diseases, and to provide a pharmaceutical composition for prophylaxis or therapy of the diseases.
SOLUTION: A substance having effects on the therapy or the prophylaxis of the diseases related to the hypoxic response can readily be screened by a method for screening a DEC1-mediating substance or a method for screening a DEC1-non-mediating substance. Furthermore, the therapy or the prophylaxis of the cancer or the ischemic diseases can be carried out by the pharmaceutical composition, comprising the screened substance or the substance promoting or inhibiting functions of the DEC1.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：分析与缺氧状态相关的疾病中与DEC1相关的缺氧反应。癌症或缺血性疾病，并提供用于预防或治疗疾病的药物组合物。解决方案：通过用于筛选DEC1介导物质的方法或用于筛选DEC1非介导物质的方法，可以容易地筛选对治疗或预防与缺氧反应相关的疾病有影响的物质。此外，可以通过包含筛选物质或促进或抑制DEC1功能的物质的药物组合物来进行癌症或缺血性疾病的治疗或预防。版权所有（C）2009，JPO＆INPIT

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