专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP1857125A4 METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION 有权转让
标题翻译：方法制造板式再生软骨的
公开(公告)号：EP1857125A4
公开(公告)日：2010-09-22
申请号：EP06714869
申请日：2006-02-28
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： KATO YUKIO , TSUJI KOICHIRO , YAMANAKA KATSUYUKI
IPC分类号： C12N5/20 , A61L27/38
CPC分类号： A61L27/18 , A61L27/3817 , A61L27/3852 , A61L27/56 , A61L2430/06 , C12N5/0655 , C12N2501/15 , C12N2501/39 , C12N2533/40 , C08L67/04

2. 发明专利

AT421887T 未知
公开(公告)号：AT421887T
公开(公告)日：2009-02-15
申请号：AT05023194
申请日：2005-10-24
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： KAWATA TOSHITSUGU , TENJO KAORU , TSUJI KOICHIRO , YAMANAKA KATSUYUKI
IPC分类号： A61K47/34 , A61F2/28 , A61K6/00 , A61K9/70 , A61K35/28 , A61K35/32 , A61K35/34 , A61L27/00 , A61L27/18 , A61L27/36 , A61L27/38 , A61L27/56 , A61L27/58 , A61P9/10 , C12N5/074
摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

3. 发明专利

DE602005012564D1 未知
公开(公告)号：DE602005012564D1
公开(公告)日：2009-03-19
申请号：DE602005012564
申请日：2005-10-24
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： KAWATA TOSHITSUGU , TENJO KAORU , TSUJI KOICHIRO , YAMANAKA KATSUYUKI
IPC分类号： A61K47/34 , A61F2/28 , A61K6/00 , A61K9/70 , A61K35/28 , A61K35/32 , A61K35/34 , A61L27/00 , A61L27/18 , A61L27/36 , A61L27/38 , A61L27/54 , A61L27/56 , A61L27/58 , A61P9/10 , C12N5/074
摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

4. 发明专利

AU2005225133B2 Sheet for guiding regeneration of mesenchymal tissue and production method thereof 未知
公开(公告)号：AU2005225133B2
公开(公告)日：2010-06-24
申请号：AU2005225133
申请日：2005-10-24
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： TSUJI KOICHIRO , KAWATA TOSHITSUGU , TENJO KAORU , YAMANAKA KATSUYUKI
IPC分类号： A61L27/38 , A61K35/28 , A61K35/32 , A61K35/34 , A61L15/24 , A61L27/00 , A61L27/18 , A61L27/22 , A61L27/36 , A61L27/56 , A61L27/58 , C12N5/074
摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

5. 发明专利

AU2006219396A1 Method of fabricating sheet for cartilage tissue regeneration 未知
公开(公告)号：AU2006219396A1
公开(公告)日：2006-09-08
申请号：AU2006219396
申请日：2006-02-28
申请人： TWO CELLS CO LTD , G C DENTAL IND CORP
发明人： YAMANAKA KATSUYUKI , TSUJI KOICHIRO , KATO YUKIO
IPC分类号： A61L27/00 , A61K9/70 , A61K35/12 , A61K35/32 , A61K35/545 , A61K47/12 , A61K47/22 , A61K47/34 , A61K47/36 , A61L27/18 , A61L27/20 , A61L27/36 , A61L27/38 , A61L27/40 , A61L27/56 , A61L27/58 , A61P19/00 , C12M3/00 , C12N5/077
摘要： An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglyc-olic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.

6. 发明专利

AU2005225133A1 Sheet for guiding regeneration of mesenchymal tissue and production method thereof 未知
公开(公告)号：AU2005225133A1
公开(公告)日：2006-05-11
申请号：AU2005225133
申请日：2005-10-24
申请人： G C DENTAL IND CORP , TWO CELLS CO LTD
发明人： TSUJI KOICHIRO , KAWATA TOSHITSUGU , TENJO KAORU , YAMANAKA KATSUYUKI
IPC分类号： A61L27/38 , A61K35/28 , A61K35/32 , A61K35/34 , A61L15/24 , A61L27/00 , A61L27/18 , A61L27/22 , A61L27/36 , A61L27/56 , A61L27/58 , C12N5/074
摘要： To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

7. 发明申请

WO2011111787A8 CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME 审中-公开
标题翻译：含有中间干细胞的细胞制备方法及其生产方法
公开(公告)号：WO2011111787A8
公开(公告)日：2011-12-01
申请号：PCT/JP2011055683
申请日：2011-03-10
申请人： TWO CELLS CO LTD , TSUCHIYA TOSHIE , TSUJI KOICHIRO , KATO YUKIO , SHAO JIN CHANG , HARA MAIKO
发明人： TSUCHIYA TOSHIE , TSUJI KOICHIRO , KATO YUKIO , SHAO JIN CHANG , HARA MAIKO
IPC分类号： A61K35/12 , A61K35/28 , A61P37/06 , C12N5/0775
CPC分类号： C12N5/0663 , A61K35/12 , C12N5/0668 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15
摘要： Disclosed is a cell preparation, which contains mesenchymal stem cells that maintain immunosuppressive ability, and which is produced by serum-free or low-serum culture. Specifically disclosed is a method for producing a cell preparation containing mesenchymal stem cells, which is characterized by comprising: a proliferation step wherein mesenchymal stem cells are proliferated in a culture medium that contains FGF, PDGF, TGF-ß, HGF, EGF, at least one phospholipid and at least one fatty acid; and a screening step wherein the mesenchymal stem cells after the proliferation step are screened for mesenchymal stem cells in which the immunosuppressive ability is maintained or improved.
摘要翻译：公开了一种细胞制剂，其含有维持免疫抑制能力的间充质干细胞，其通过无血清或低血清培养产生。具体公开了一种含有间充质干细胞的细胞制剂的制造方法，其特征在于，包括：增殖步骤，其中间充质干细胞至少在含有FGF，PDGF，TGF-β，HGF，EGF的培养基中增殖一种磷脂和至少一种脂肪酸; 筛选步骤，其中对增殖步骤后的间充质干细胞进行筛选，其中维持或改善免疫抑制能力的间充质干细胞。

8. 发明申请

WO2004081174A3 MARKER FOR DETECTING MESENCHYMAL STEM CELL AND METHOD OF DISTINGUISHING MESENCHYMAL STEM CELL USING THE MARKER 审中-公开
标题翻译：用于检测中胚层干细胞的标记物和使用标记鉴别骨髓干细胞的方法
公开(公告)号：WO2004081174A3
公开(公告)日：2004-12-02
申请号：PCT/JP2004002457
申请日：2004-02-27
申请人： JAPAN SCIENCE & TECH AGENCY , KATO YUKIO , TWO CELLS CO LTD , TSUJI KOICHIRO , KOIKE CHIKA
发明人： KATO YUKIO , TSUJI KOICHIRO , KOIKE CHIKA
IPC分类号： G01N33/53 , C07K14/47 , C07K16/18 , C12N20060101 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/113 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N37/00 , C12N15/11
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： It is intended to provide a marker for detecting, separating and distinguishing a mesenchymal stem cell and a method of detecting, separating and distinguishing a mesenchymal stem cell by using this marker. A gene shown in Sequence Listing is expressed specifically in a mesenchymal stem cell. This gene is employed as a marker for detecting a mesenchymal stem cell. It is further intended to provide a probe for detecting the mesenchymal stem cell marker gene and a PCR primer for amplifying the gene in a sample cell in detecting the mesenchymal stem cell gene marker. It is furthermore intended to provide a polypeptide marker for detecting a mesenchymal stem cell comprising a polypeptide in which the above-described mesenchymal stem cell marker gene is expressed, an antibody binding specifically to the polypeptide marker for detecting the polypeptide marker, a probe for detecting the mesenchymal stem cell marker gene, and a method of distinguishing and separating a mesenchymal stem cell by using the antibody binding specifically to the polypeptide marker.
摘要翻译：旨在提供用于检测，分离和鉴别间充质干细胞的标记物和通过使用该标记物检测，分离和区分间充质干细胞的方法。序列表中所示的基因特异性地在间充质干细胞中表达。该基因用作检测间充质干细胞的标记物。还旨在提供用于检测间充质干细胞标记基因的探针和用于在检测间充质干细胞基因标记物中扩增样品细胞中的基因的PCR引物。此外，还提供了用于检测间充质干细胞的多肽标记物，其包含其中表达上述间充质干细胞标记基因的多肽，特异性结合用于检测多肽标记的多肽标记物的抗体，检测用探针间充质干细胞标记基因，以及通过使用与多肽标记特异性结合的抗体来区分和分离间充质干细胞的方法。

9. 发明专利

JP2011067175A Method for culturing mesenchymal stem cell 有权
标题翻译：培养细胞干细胞的方法
公开(公告)号：JP2011067175A
公开(公告)日：2011-04-07
申请号：JP2009222981
申请日：2009-09-28
申请人： Gc Corp , Two Cells Co Ltd , 株式会社ジーシー , 株式会社ツーセル
发明人： SAKAI YUUDAI , YAMANAKA KATSUYUKI , TAKEDA MIKA , OKURA TOMOHISA , TSUJI KOICHIRO
IPC分类号： C12N5/07
CPC分类号： C12N5/0663 , C12N2500/50
摘要： PROBLEM TO BE SOLVED: To provide a method for culturing mesenchymal stem cells, which efficiently selects and proliferates mesenchymal stem cells without requiring a separator of exclusive use and complicated separation operation. SOLUTION: One or more of bone marrow liquid, cord blood, peripheral blood, synovial membrane and amnion are seeded in a liquid medium that is filled in a container and comprises water having a specific gravity of 1.06-1.10 g/mL at 37°C as a main component and a culture is carried out on the ceiling surface of the container at 37±2°C to culture the mesenchymal stem cells. Preferably the specific gravity of the liquid medium is adjusted by using at least one selected from silica fine powder coated with a polyvinylpyrrolidone, a water-soluble copolymer of sucrose and epichlorohydrin and a water-soluble compound containing a triiodo aromatic ring. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供一种培养间充质干细胞的方法，其能够有效地选择和增殖间充质干细胞，而不需要专用分离器和复杂的分离操作。解决方案：将一种或多种骨髓液，脐带血，外周血，滑膜和羊膜接种在装在容器中的液体培养基中，并包含比重为1.06-1.10g / mL的水 37℃作为主要成分，并在37±2℃的容器的顶棚表面进行培养，以培养间充质干细胞。优选地，通过使用选自涂覆有聚乙烯吡咯烷酮的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和含有三碘芳环的水溶性化合物中的至少一种来调节液体介质的比重。版权所有（C）2011，JPO＆INPIT

10. 发明专利

JP2012143158A Inducible mesenchymal stem cell and method for preparing the same 审中-公开
标题翻译：可诱导的中胚层干细胞及其制备方法
公开(公告)号：JP2012143158A
公开(公告)日：2012-08-02
申请号：JP2009115378
申请日：2009-05-12
申请人： Hiroshima Univ , Two Cells Co Ltd , 国立大学法人広島大学 , 株式会社ツーセル
发明人： KATO YUKIO , KAWAMOTO TAKESHI , NISHIMURA MASAHIRO , TSUJI KOICHIRO
IPC分类号： C12N5/10 , C12N15/09 , C12Q1/68
CPC分类号： C12N5/0662 , C12N2510/00
摘要： PROBLEM TO BE SOLVED: To provide a cell having self-renewal potency and differentiation potency similar to those of a mesenchymal stem cell (MSC) without utilizing a human embryo or embryonic stem cell (ES cell).SOLUTION: There are provided a method for preparing an inducible MSC according to a method including a step of introducing at least one of genes having a specific base sequence into a cell; a kit for preparation; a method for detecting the inducible MSC by detecting expression of the gene; a kit for detection; a microarray for the detection; and a method for screening a factor controlling the differentiation potency of the MSC.
摘要翻译：要解决的问题：提供具有与间充质干细胞（MSC）类似的自我更新效力和分化能力的细胞，而不利用人胚胎或胚胎干细胞（ES细胞）。解决方案：提供了一种根据包括将至少一个具有特定碱基序列的基因导入细胞的方法制备可诱导MSC的方法，一套准备工具; 通过检测该基因的表达来检测诱导型MSC的方法; 检测用试剂盒用于检测的微阵列; 以及筛选控制MSC的分化潜能的因素的方法。版权所有（C）2012，JPO＆INPIT

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式