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    • 1. 发明申请
    • Substrates for hybridization and method of using the same
    • 用于杂交的底物及其使用方法
    • US20060084058A1
    • 2006-04-20
    • US10508691
    • 2003-03-27
    • Fumio NakamuraMasahiko HaraJunko Hayashi
    • Fumio NakamuraMasahiko HaraJunko Hayashi
    • C12Q1/68C12M1/34
    • C12Q1/6837C12Q2565/525C12Q2565/518
    • A hybridization substrate having on a substrate surface thereof a branching nucleic acid strand comprising a principal strand of nucleic acid a portion of which is attached to the surface of the substrate and a portion of which is single-stranded, and a strand of single-stranded nucleic acid for probe that is partially hybridized to a portion of the single-stranded portion of the principal nucleic acid. A hybridization substrate having on a substrate surface thereof a branching nucleic acid strand comprising a principal strand of nucleic acid a portion of which is attached to the surface of the substrate and a portion of which is single-stranded; an accessory strand of nucleic acid that is single-stranded and partially hybridized to a portion of the single-stranded portion of the principal strand of nucleic acid; a strand of single-stranded nucleic acid for probe partially hybridized to a portion of the single-stranded portion of the principal strand of nucleic acid; and/or a strand of single-stranded nucleic acid for probe partially hybridized to a portion of the accessory strand of nucleic acid. A method comprising bringing target nucleic acid into contact with a surface upon which has been immobilized the branching nucleic acid strand of the above-mentioned substrate to test the complementarity of the target nucleic acid with the probe area of the branching nucleic acid strand. Provided are a hybridization substrate upon the surface of which DNA strands are immobilized and a complementarity test method employing this substrate.
    • 一种杂交基质,其在其基材表面上具有支链核酸链,其包含核酸的主链,其一部分连接到所述底物的表面,并且其一部分是单链的,并且单链的链 与主要核酸的单链部分的一部分部分杂交的探针的核酸。 一种杂交基质,其在其基材表面上具有分支核酸链,其包含核酸的主链,其一部分附着于基底的表面,其一部分是单链的; 与核酸的主链的单链部分的一部分单链并部分杂交的核酸的辅助链; 用于探针的单链核酸链与核酸主链的单链部分的一部分杂交; 和/或用于探针的单链核酸链与核酸的辅助链的一部分杂交。 一种方法,包括使靶核酸与固定有上述底物的支化核酸链的表面接触,以测试靶核酸与分支核酸链的探针区的互补性。 提供了在其表面上固定有DNA链的杂交底物和使用该底物的互补性测试方法。
    • 2. 发明授权
    • Substrate for detecting base sequences, method of manufacturing the substrate, and method of detecting base sequences using the substrate
    • 用于检测碱基序列的底物,制备该底物的方法,以及使用该底物检测碱基序列的方法
    • US06495328B2
    • 2002-12-17
    • US09853948
    • 2001-05-11
    • Fumio NakamuraMasahiko Hara
    • Fumio NakamuraMasahiko Hara
    • C12Q168
    • B82Y30/00C12Q1/6834G01N21/553
    • A substrate for detecting base sequences that comprises a transparent support, a thin metal film formed on one side of the transparent support, and a self-assembled monolayer with a nonionic aromatic compound being an intercalator of nucleic acid polymers dispersed over the surface formed on the metal film. The substrate is manufactured, for example, by immersing a transparent support with a thin gold film formed on one side in a solution that contains a disulfide (S—S) compound containing anthracene for the intercalator of nucleic acid polymers form on the metal film. A nucleic acid polymer (probe or target) is immobilized to the monolayer of the substrate, another nucleic acid polymer (target or probe) is hybridized to the nucleic acid polymer immobilized to the monolayer, and the results of the hybridization can be detected by the Surface Plasmon Resonance method after washing.
    • 用于检测碱基序列的基板,其包含透明支撑体,形成在透明支撑体的一侧上的薄金属膜,以及自组装单层,其中非离子芳族化合物是分散在形成在所述透明支撑体上的表面上的核酸聚合物的嵌入剂 金属膜。 基板例如通过将一面形成的薄金膜的透明支撑体浸渍在含有用于核酸聚合物嵌嵌剂的二硫化物(S-S)化合物的溶液中而形成的金属膜上。 将核酸聚合物(探针或靶)固定在底物的单层上,另一种核酸聚合物(靶或探针)与固定在单层上的核酸聚合物杂交,杂交结果可以通过 表面等离子体共振法洗涤后。
    • 5. 发明授权
    • Vacuum film-forming apparatus
    • 真空成膜装置
    • US07828900B2
    • 2010-11-09
    • US12111698
    • 2008-04-29
    • Masanobu HatanakaMichio IshikawaSe-Ju LimFumio Nakamura
    • Masanobu HatanakaMichio IshikawaSe-Ju LimFumio Nakamura
    • C23C16/00C23C14/00C23F1/00H01L21/306
    • C23C16/38C23C16/0236C23C16/34C23C16/44C23C16/4405C23C16/45525C23C16/45536C23C16/45551C23C16/54Y10S414/139
    • A vacuum film-forming apparatus comprising substrate stages; vacuum chamber-forming containers opposed to the stages; a means for moving the substrate between the stages; and gas-introduction means connected to every containers, wherein one of the stage and the container is ascended or descended towards the other to bring the upper face of the stage and the opening of the container into contact with one another so that vacuum chambers can be formed and that a raw gas and/or a reactant gas can be introduced into each space of the chamber through each gas-introduction means to carry out either the adsorption or reaction step for allowing the raw gas to react with the reactant gas. The apparatus permits the independent establishment of process conditions for the adsorption and reaction processes and the better acceleration of the reaction between raw and reactant gases to give a film having excellent quality and the apparatus can be manufactured at a low cost.
    • 一种包括基底台的真空成膜装置; 与阶段相对的真空室形成容器; 用于在所述级之间移动所述衬底的装置; 以及连接到每个容器的气体导入装置,其中所述平台和容器中的一个朝着另一个上升或下降以使所述台的上表面和所述容器的开口彼此接触,使得真空室可以 并且可以通过每个气体引入装置将原料气体和/或反应气体引入室的每个空间中,以进行用于使原料气体与反应气体反应的吸附或反应步骤。 该装置允许独立地建立用于吸附和反应过程的工艺条件,并且更好地加速原料气体和反应气体之间的反应,得到具有优良品质的膜,并且可以以低成本制造装置。
    • 8. 发明授权
    • Process for producing sialon
    • 赛隆生产工艺
    • US4731236A
    • 1988-03-15
    • US852436
    • 1986-04-15
    • Norihiro MurakawaKazuyoshi IsogayaKensaku MaruyamaFumio Nakamura
    • Norihiro MurakawaKazuyoshi IsogayaKensaku MaruyamaFumio Nakamura
    • C04B35/626C01B21/082C04B35/597C01B33/26
    • C01B21/0826C04B35/597C01P2004/62C01P2006/80
    • A process for producing a sialon powder, which process comprises the steps of:introducing a decomposable silicon compound, decomposable aluminum compound and a decomposable carbon compound into a steam-containing hot gas to decompose said decomposable silicon compound, aluminum compound and carbon compound in said hot gas into silicon oxide, aluminum oxide and elemental carbon, respectively, thereby producing a fine solid particle mixture consisting essentially of said silicon oxide, aluminum oxide and elemental carbon to be dispersed in said gas;collecting said fine solid particle mixture dispersed in the gas from the gas phase by a solid-gas separating technique; andcalcining the thus-obtained carbon-containing composition consisting essentially of said silicon oxide, aluminum oxide and elemental carbon, in a nitrogen containing gas atmosphere.
    • 一种赛隆粉末的制造方法,该方法包括以下步骤:将可分解硅化合物,可分解铝化合物和可分解碳化合物引入含蒸汽的热气中,以分解所述可分解硅化合物,所述铝化合物和碳化合物 热气分别分成氧化硅,氧化铝和元素碳,从而产生基本上由所述氧化硅,氧化铝和元素碳组成的细固体颗粒混合物分散在所述气体中; 通过固相气体分离技术从气相中收集分散在气体中的细固体颗粒混合物; 并在含氮气体气氛中煅烧由此获得的基本上由所述氧化硅,氧化铝和元素碳组成的含碳组合物。
    • 9. 发明授权
    • Slice level deciding circuit
    • 切片电平判定电路
    • US4672682A
    • 1987-06-09
    • US783019
    • 1985-10-02
    • Seishiro NaruseFumio Nakamura
    • Seishiro NaruseFumio Nakamura
    • G06T1/00H04N1/403G06K9/38
    • H04N1/403
    • A slice level deciding circuit includes a slice level generator for generating a variable slice level and a comparator for comparing a slice level generated from the generator with a picture image signal. An optimum slice level can be provided by operating, via an arithmetic circuit, a prescribed value on the same slice level as generated from the generator when an output from the comparator satisfies a prescribed slice level deciding condition. Accordingly, deterioration of a picture image quality can be prevented, which is caused by changes of a quantity of light due to variations of ambient temperature around the light source used for obtaining a picture image signal from the readout medium and noise produced upon reading portions on the readout medium where a quantity of light from the light source is reduced.
    • 限幅电平决定电路包括用于产生可变限幅电平的限幅电平发生器和用于将从发生器产生的限幅电平与图像信号进行比较的比较器。 当比较器的输出满足规定的限幅电平判定条件时,可以通过运算电路在与发生器相同的限幅电平上进行规定的值的操作来提供最佳限幅电平。 因此,可以防止图像质量的劣化,这是由于用于从读出介质获得图像信号的光源周围的环境温度的变化引起的光量的变化和读取部分上产生的噪声引起的图像质量劣化 其中来自光源的光量减少的读出介质。