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1. 发明申请

WO2018054970A2 POL6 POLYMERASE VARIANTS 审中-公开
标题翻译： POL6聚合酶变体
公开(公告)号：WO2018054970A2
公开(公告)日：2018-03-29
申请号：PCT/EP2017/073767
申请日：2017-09-20
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE SEQUENCING SOLUTIONS, INC. , ROCHE MOLECULAR SYSTEMS, INC.
发明人： BOENITZ-DULAT, Mara , SUKO, Shawn , AYER, Aruna , WOERSDOERFER, Bigna , ECKERT, Barbara , SARVABHOWMAN, Preethi , SCHWAB, Charles Wayan , WUNDERLICH, David , CLEOMA, Arnold , LEDERMAN, Ilya , THAI, Eileen , MCGAW, Colin , AMATO, Evan
IPC分类号： C12N9/12
摘要： The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.
摘要翻译：本公开内容提供了变体Pol6聚合酶多肽，包含Pol6变体多肽的组合物，以及使用变体Pol6多肽来确定核酸测序的方法，例如通过纳米孔测序。变体Pol6聚合酶具有降低的来自聚合酶 - 模板复合物的模板解离速率，这导致相对于它们所源自的亲本Pol6多肽而言增加的持续合成能力。

2. 发明申请

WO2018054970A3 POL6 POLYMERASE VARIANTS 审中-公开
公开(公告)号：WO2018054970A3
公开(公告)日：2018-03-29
申请号：PCT/EP2017/073767
申请日：2017-09-20
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE SEQUENCING SOLUTIONS, INC. , ROCHE MOLECULAR SYSTEMS, INC.
发明人： BOENITZ-DULAT, Mara , SUKO, Shawn , AYER, Aruna , WOERSDOERFER, Bigna , ECKERT, Barbara , SARVABHOWMAN, Preethi , SCHWAB, Charles Wayan , WUNDERLICH, David , CLEOMA, Arnold , LEDERMAN, Ilya , THAI, Eileen , MCGAW, Colin , AMATO, Evan
IPC分类号： C12N9/12
摘要： The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.

3. 发明申请

WO2016124543A1 POLYMERASE VARIANTS 审中-公开
标题翻译：聚合物变体
公开(公告)号：WO2016124543A1
公开(公告)日：2016-08-11
申请号：PCT/EP2016/052068
申请日：2016-02-01
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE MOLECULAR SYSTEMS, INC. , GENIA TECHNOLOGIES, INC.
发明人： AYER, Aruna , BIBILLO, Arkadiusz , ECKERT, Barbara , SUKO, Shawn , ARNOLD, Cleoma Renetta , SCHWAB, Charles Wayan , THAI, Eileen , LEDERMAN, Ilya , MCGAW, Colin Alexander , SCHULTZ, Tyler O'Brien , BOENITZ-DULAT, Mara , WOERSDOERFER, Bigna , WUNDERLICH, David Daniel
IPC分类号： C12N9/00
CPC分类号： C12N9/1252 , C07K2319/21 , C12Y207/07007
摘要： Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
摘要翻译：本文描述的是具有至少一个选自H223，N224，Y225，H227，I295，Y342，T343，I357，S360，L361，I363，S365Q，S366，Y367，P368，D417，E475，Y476， F478，K518，H527，T529，M531，N535，G539，P542，N545，Q546，A547，L549，I550，N552，G553，F558，A596，G603，A610，V615，Y622，C623，D624，I628，Y629， R632，N635，M641，A643，I644，T647，I648，T651，I652，K655，W656，D657，V658，H660，F662，L690及其组合。

4. 发明申请

WO2022069461A1 BACTERIAL PILUS PROTEIN COMPLEX FIMGT-DSF STABILIZED PROTEIN COMPLEXES FOR PRODUCING FILAMENTOUS PHAGES 审中-公开
公开(公告)号：WO2022069461A1
公开(公告)日：2022-04-07
申请号：PCT/EP2021/076623
申请日：2021-09-28
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS GMBH , ROCHE DIAGNOSTICS OPERATIONS, INC.
发明人： BOENITZ-DULAT, Mara , SCHRAEML, Michael , WOERSDOERFER, Bigna
IPC分类号： C12N15/10 , C07K14/245 , C07K14/24 , C07K2319/70 , C12N15/1037
摘要： The present invention relates to bacterial pilus protein complex FimGt-DsF stabilized protein complexes for producing phagemids or filamentous phages, and methods for use of these.

5. 发明申请

WO2017109003A1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM ALCALIGENES FAECALIS AS WELL AS METHODS AND USES INVOLVING THE SAME 审中-公开
标题翻译：来自ALCALIGENES FAECALIS的突变型3-羟基丁酸脱氢酶以及涉及其的方法和用途
公开(公告)号：WO2017109003A1
公开(公告)日：2017-06-29
申请号：PCT/EP2016/082257
申请日：2016-12-21
申请人： F. HOFFMANN-LA ROCHE AG , ROCHE DIAGNOSTICS OPERATIONS, INC. , ROCHE DIABETES CARE, INC. , ROCHE DIAGNOSTICS GMBH , ROCHE DIABETES CARE GMBH
发明人： KRATZSCH, Peter , BOENITZ-DULAT, Mara , BECK, Daniela , STREIDL, Thomas , HUNT DUVALL, Stacy
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12Y101/0103
摘要： The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, the use of the mutant 3-HBDH for determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.
摘要翻译：本发明涉及相对于野生型3-HBDH（编码突变体3-HBDH的核酸）具有改善的性能的突变体3-羟基丁酸酯脱氢酶（3-HBDH），所述细胞包含突变体3-HBDH或核酸，确定样品中3-羟基丁酸的量或浓度的方法，突变体3-HBDH用于确定样品中3-羟基丁酸的量或浓度的用途，以及用于确定样品中3-羟基丁酸的量或浓度的装置。

6. 发明申请

WO2017137491A1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM RHODOBACTER SPHAEROIDES AS WELL AS METHODS AND USES INVOLVING THE SAME 审中-公开
标题翻译：来自球形芽孢杆菌的突变3-羟基丁酸脱氢酶以及涉及相同的方法和用途
公开(公告)号：WO2017137491A1
公开(公告)日：2017-08-17
申请号：PCT/EP2017/052855
申请日：2017-02-09
申请人： ROCHE DIABETES CARE GMBH , ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , ROCHE DIABETES CARE, INC. , ROCHE DIAGNOSTICS OPERATIONS, INC.
发明人： BECK, Daniela , BOENITZ-DULAT, Mara , KRATZSCH, Peter , STREIDL, Thomas , HORN, Carina
IPC分类号： C12N9/04 , C12Q1/32
CPC分类号： C12N9/0006 , C12Q1/32 , C12Y101/0103
摘要： The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3- HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3- hydroxybutyrate in a sample, and a device for determining the amount or concentration of3-hydroxybutyratein a sample.
摘要翻译：本发明涉及相对于野生型3-HBDH（编码突变体3-HBDH的核酸）性能改善的突变体3-羟基丁酸脱氢酶（3-HBDH），所述细胞包含突变体3-HBDH或核酸，确定样品中3-羟基丁酸的量或浓度的方法，以及确定样品中3-羟基丁酸的量或浓度的装置。

7. 发明申请

WO2011072833A1 A MUTANT SERINE PROTEASE AND A METHOD AND USES RELATING THERETO 审中-公开
标题翻译：突变体丝氨酸蛋白酶及其相关方法和用途
公开(公告)号：WO2011072833A1
公开(公告)日：2011-06-23
申请号：PCT/EP2010/007582
申请日：2010-12-13
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , BOENITZ-DULAT, Mara , CIREFICE, Morgane
发明人： BOENITZ-DULAT, Mara , CIREFICE, Morgane
IPC分类号： C12N9/64
CPC分类号： C12N9/6424
摘要： In a first aspect, the present invention relates to a mutant serine protease having glutamyl endopeptidase activity, comprising an amino acid sequence that is at least 90 % identical to the amino acid sequence of SEQ ID NO: 1 and comprising an amino acid substitution at the position corresponding to position G166 of SEQ ID NO: 1. The present invention further relates to a polynucleotide encoding a mutant serine protease of the invention, to an expression vector comprising said polynucleotide, as well as to a host cell comprising the expression vector of the present invention. In yet another aspect, the present invention provides a method of determining the amount of HbAIc, and a use and a kit related thereto. In a further aspect, the invention relates to a use of a mutant serine protease as defined in the context of the present invention for enzymatically cleaving a polypeptide.
摘要翻译：在第一方面，本发明涉及具有谷氨酰肽链内切酶活性的突变丝氨酸蛋白酶，其包含与SEQ ID NO：1的氨基酸序列至少90％相同的氨基酸序列，并且包含在位置对应于SEQ ID NO：1的位置G166。本发明还涉及编码本发明的突变丝氨酸蛋白酶的多核苷酸，包含所述多核苷酸的表达载体，以及包含所述多核苷酸的表达载体的宿主细胞本发明。在另一方面，本发明提供了确定HbAIc量的方法，以及与其相关的用途和试剂盒。在另一方面，本发明涉及本发明上下文中定义的突变丝氨酸蛋白酶用于酶切割多肽的用途。

8. 发明申请

WO2007118647A1 IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 审中-公开
标题翻译：吡咯烷醇喹诺酮类可溶性葡萄糖脱氢酶的改良突变体
公开(公告)号：WO2007118647A1
公开(公告)日：2007-10-25
申请号：PCT/EP2007/003207
申请日：2007-04-11
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
发明人： BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译：本发明公开了与麦芽糖相比具有改善的葡萄糖特异性的PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC1.1.5.2）的突变体，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体另外包含至少一个用于改善突变体的稳定性的突变和用于改善突变体对葡萄糖的亲和力的一种或多种突变，和/或用于进一步提高突变体对葡萄糖的特异性的一个或多个突变葡萄糖与麦芽糖相比的突变体，其中位置348对应于从A.ccalcoaceticuss-GDH野生型序列已知的氨基酸位置。还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。

9. 发明申请

WO2006008132A1 GENETICALLY ENGINEERED PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE COMPRISING AN AMINO ACID INSERTION 审中-公开
标题翻译：基因工程化的吡咯烷醇喹诺酮依赖葡萄糖脱水酶包含氨基酸插入
公开(公告)号：WO2006008132A1
公开(公告)日：2006-01-26
申请号：PCT/EP2005/007844
申请日：2005-07-19
申请人： ROCHE DIAGNOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , BOENITZ-DULAT, Mara , LAGGERBAUER, Jessica , SCHMUCK, Rainer , KRATZSCH, Peter , KNAPPE, Wolfgang-Reinhold
发明人： BOENITZ-DULAT, Mara , LAGGERBAUER, Jessica , SCHMUCK, Rainer , KRATZSCH, Peter , KNAPPE, Wolfgang-Reinhold
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant sGDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
摘要翻译：本发明涉及可溶性吡咯并喹啉醌（PQQ） - 依赖性葡萄糖脱氢酶（s-GDH）的改进变体，其包含位置428和429之间的氨基酸插入，其对应于从

10. 发明公开

EP3253867A1 POLYMERASE VARIANTS 有权
标题翻译：聚合酶变体
公开(公告)号：EP3253867A1
公开(公告)日：2017-12-13
申请号：EP16703461.0
申请日：2016-02-01
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： AYER, Aruna , BIBILLO, Arkadiusz , ECKERT, Barbara , SUKO, Shawn , ARNOLD, Cleoma Renetta , SCHWAB, Charles Wayan , THAI, Eileen , LEDERMAN, Ilya , MCGAW, Colin Alexander , SCHULTZ, Tyler O'Brien , BOENITZ-DULAT, Mara , WOERSDOERFER, Bigna , WUNDERLICH, David Daniel
IPC分类号： C12N9/00
摘要： Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

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