专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07588889B2 Chemical arrays and methods of using the same 失效
标题翻译：化学数组及其使用方法
公开(公告)号：US07588889B2
公开(公告)日：2009-09-15
申请号：US11008603
申请日：2004-12-08
申请人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
发明人： Paul K. Wolber , Robert H. Kincaid , Douglas Amorese , Diane Ilsley-Tyree , Andrew S. Atwell , Mel N. Kronick , Eric M. Leproust
IPC分类号： C12Q1/68 , C12M1/00 , C07H21/04
CPC分类号： C12Q1/6883 , B01J2219/00497 , B01J2219/00572 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00722 , B82Y30/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6876 , C12Q2600/158 , C40B40/06 , C40B50/14 , C40B70/00 , C12Q2565/537 , C12Q2525/161 , C12Q2565/525 , C12Q2525/131 , C12Q2521/301
摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
摘要翻译：提供了从初始化学阵列产生产物分子的混合物的方法和组合物。在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。

2. 发明授权

US07534561B2 Nucleic acid array in situ fabrication methods and arrays produced using the same 有权
标题翻译：核酸阵列原位制备方法和使用其制备的阵列
公开(公告)号：US07534561B2
公开(公告)日：2009-05-19
申请号：US10407080
申请日：2003-04-02
申请人： Theodore R. Sana , Eric M. Leproust , Michel G. M. Perbost , Paul K. Wolber
发明人： Theodore R. Sana , Eric M. Leproust , Michel G. M. Perbost , Paul K. Wolber
IPC分类号： C12Q1/68 , C12N11/16 , C07H21/04 , C12P19/34
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/0036 , B01J2219/00378 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00695 , B01J2219/00707 , B01J2219/00722 , C12Q1/6837 , C40B40/06 , C40B50/14 , C40B60/14 , C12Q2565/513 , C12Q2545/101
摘要： Methods and devices for producing a nucleic acid arrays using in situ nucleic acid array synthesis protocols are provided. A feature of certain embodiments of the invention is that control probes are produced in collections of features, e.g., columns, of the arrays that have been selected according to a particular efficient control probe feature/column selection protocol. A feature of certain other embodiments of the invention is that an “all-bases-all-layers” probe set is produced in at least one of column of the arrays. Also provided are devices configured for use in the subject methods, as well as arrays produced using the subject methods and devices as well as methods for using such arrays.
摘要翻译：提供了使用原位核酸阵列合成方案制备核酸阵列的方法和装置。本发明的某些实施例的特征在于，控制探针是根据特定的有效控制探针特征/列选择协议选择的阵列的特征（例如列）的集合产生的。本发明的某些其他实施例的特征在于，在全部阵列中的至少一列中产生“全基底全层”探针组。还提供了被配置为在主题方法中使用的装置以及使用主题方法和装置产生的阵列以及使用这些阵列的方法。

3. 发明授权

US07302348B2 Method and system for quantifying and removing spatial-intensity trends in microarray data 有权
标题翻译：用于量化和消除微阵列数据中的空间强度趋势的方法和系统
公开(公告)号：US07302348B2
公开(公告)日：2007-11-27
申请号：US10859868
申请日：2004-06-02
申请人： Jayati Ghosh , Bill J. Peck , Eric M. Leproust , Charles David Troup , Glenda Choate Delenstarr , Patrick J. Collins , John F. Corson , Paul K. Wolber , Xiangyang Zhou
发明人： Jayati Ghosh , Bill J. Peck , Eric M. Leproust , Charles David Troup , Glenda Choate Delenstarr , Patrick J. Collins , John F. Corson , Paul K. Wolber , Xiangyang Zhou
IPC分类号： G06F19/00 , G06F15/00 , G11C17/00
CPC分类号： G06F19/24 , G06F19/20
摘要： A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.
摘要翻译：一种用于量化和校正具有一个或多个通道的微阵列数据集的每个通道的空间强度趋势的方法和系统。本发明的一个实施例的方法和系统从微阵列数据集的每个通道中选择一组特征。基于所选择的特征集合，使用表面来确定微阵列数据集的每个通道中的所有特征的强度。基于微阵列数据集的每个通道的表面到强度，量化微阵列数据集内的空间强度趋势。在确定表面后，可以从微阵列数据集中去除空间强度趋势。

4. 发明授权

US07504213B2 Methods and apparatus for preparing arrays comprising features having degenerate biopolymers 失效
标题翻译：用于制备包括具有简并生物聚合物的特征的阵列的方法和装置
公开(公告)号：US07504213B2
公开(公告)日：2009-03-17
申请号：US10722155
申请日：2003-11-25
申请人： Theodore R. Sana , Eric M. Leproust , Paul K. Wolber
发明人： Theodore R. Sana , Eric M. Leproust , Paul K. Wolber
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6813
摘要： Methods and apparatus are disclosed for synthesizing a plurality of biopolymers at predetermined feature locations on a surface of a substrate. One or more of the feature locations comprises degenerate biopolymers. One or more biopolymer subunit precursors are added, in multiple rounds of subunit additions, at each of multiple feature locations on the surface to form the plurality of biopolymers on the surface. For each feature location comprising degenerate biopolymers, the biopolymer subunit precursors comprise a mixture of biopolymer subunit precursors for forming the degenerate biopolymers at the feature location.
摘要翻译：公开了用于在基材表面上的预定特征位置合成多种生物聚合物的方法和装置。一个或多个特征位置包括简并生物聚合物。在表面上的多个特征位置的每一个处，在多轮亚基添加物中加入一种或多种生物聚合物亚基前体，以在表面上形成多种生物聚合物。对于包含简并生物聚合物的每个特征位置，生物聚合物亚基前体包含用于在特征位置形成简并生物聚合物的生物聚合物亚基前体的混合物。

5. 发明授权

US06461816B1 Methods for controlling cross-hybridization in analysis of nucleic acid sequences 失效
标题翻译：在核酸序列分析中控制交叉杂交的方法
公开(公告)号：US06461816B1
公开(公告)日：2002-10-08
申请号：US09350969
申请日：1999-07-09
申请人： Paul K. Wolber , Robert H. Kincaid
发明人： Paul K. Wolber , Robert H. Kincaid
IPC分类号： C12Q168
CPC分类号： C12Q1/6813
摘要： Methods, reagents and kits are disclosed for selecting target-specific oligonucleotide probes, which may be used in analyzing a target nucleic acid sequence. In one aspect the present invention is directed to selecting a set of target-specific oligonucleotide probes. A cross-hybridization oligonucleotide probe is identified based on a candidate target-specific oligonucleotide probe for the target nucleic acid sequence. The cross-hybridization oligonucleotide probe measures the extent of occurrence of a cross-hybridization event having a predetermined probability. Cross-hybridization results are determined employing the cross-hybridization oligonucleotide probe and the target-specific oligonucleotide probe. The target-specific oligonucleotide probe is selected or rejected for the set based on the cross-hybridization results.
摘要翻译：公开了用于选择可用于分析靶核酸序列的靶特异性寡核苷酸探针的方法，试剂和试剂盒。一方面，本发明涉及选择一组靶特异性寡核苷酸探针。基于目标核酸序列的候选靶特异性寡核苷酸探针鉴定交叉杂交寡核苷酸探针。交叉杂交寡核苷酸探针测量具有预定概率的交叉杂交事件的发生程度。使用交叉杂交寡核苷酸探针和靶特异性寡核苷酸探针测定交叉杂交结果。基于交叉杂交结果，针对该组选择或拒绝靶特异性寡核苷酸探针。

6. 发明授权

US06251588B1 Method for evaluating oligonucleotide probe sequences 失效
标题翻译：评价寡核苷酸探针序列的方法
公开(公告)号：US06251588B1
公开(公告)日：2001-06-26
申请号：US09021701
申请日：1998-02-10
申请人： Karen W. Shannon , Paul K. Wolber , Glenda C. Delenstarr , Peter G. Webb , Robert H. Kincaid
发明人： Karen W. Shannon , Paul K. Wolber , Glenda C. Delenstarr , Peter G. Webb , Robert H. Kincaid
IPC分类号： C12Q168
CPC分类号： G06F19/20 , G06F19/24
摘要： Methods are disclosed for predicting the potential of an oligonucleotide to hybridize to a target nucleotide sequence. A predetermined number of unique oligonucleotides is identified. The unique oligonucleotides are chosen to sample the entire length of a nucleotide sequence that is hybridizable with the target nucleotide sequence. At least one parameter that is independently predictive of the ability of each of the oligonucleotides of the set to hybridize to the target nucleotide sequence is determined and evaluated for each of the above oligonucleotides. A subset of oligonucleotides within the predetermined number of unique oligonucleotides is identified based on the evaluation of the parameter. Oligonucleotides in the subset are identified that are clustered along a region of the nucleotide sequence that is hybridizable to the target nucleotide sequence. The method may be carried out with the aid of a computer.
摘要翻译：公开了用于预测寡核苷酸与靶核苷酸序列杂交的潜力的方法。鉴定出预定数量的唯一寡核苷酸。选择独特的寡核苷酸来对可与靶核苷酸序列杂交的核苷酸序列的整个长度进行采样。确定并评估每种上述寡核苷酸的至少一个独立地预测该组寡核苷酸与靶核苷酸序列杂交的能力的参数。基于参数的评估来鉴定预定数量的唯一寡核苷酸内的寡核苷酸的子集。鉴定了亚群中的寡核苷酸，其沿着与靶核苷酸序列可杂交的核苷酸序列的区域聚集。该方法可以借助于计算机进行。

7. 发明授权

US07344831B2 Methods for controlling cross-hybridization in analysis of nucleic acid sequences 失效
标题翻译：在核酸序列分析中控制交叉杂交的方法
公开(公告)号：US07344831B2
公开(公告)日：2008-03-18
申请号：US10266474
申请日：2002-10-07
申请人： Paul K. Wolber , Robert H. Kincaid
发明人： Paul K. Wolber , Robert H. Kincaid
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813
摘要： Methods, reagents and kits are disclosed for selecting target-specific oligonucleotide probes, which may be used in analyzing a target nucleic acid sequence. In one aspect the present invention is directed to selecting a set of target-specific oligonucleotide probes. A cross-hybridization oligonucleotide probe is identified based on a candidate target-specific oligonucleotide probe for the target nucleic acid sequence. The cross-hybridization oligonucleotide probe measures the extent of occurrence of a cross-hybridization event having a predetermined probability. Cross-hybridization results are determined employing the cross-hybridization oligonucleotide probe and the target-specific oligonucleotide probe. The target-specific oligonucleotide probe is selected or rejected for the set based on the cross-hybridization results.
摘要翻译：公开了用于选择可用于分析靶核酸序列的靶特异性寡核苷酸探针的方法，试剂和试剂盒。一方面，本发明涉及选择一组靶特异性寡核苷酸探针。基于目标核酸序列的候选靶特异性寡核苷酸探针鉴定交叉杂交寡核苷酸探针。交叉杂交寡核苷酸探针测定具有预定概率的交叉杂交事件的发生程度。使用交叉杂交寡核苷酸探针和靶特异性寡核苷酸探针测定交叉杂交结果。基于交叉杂交结果，针对该组选择或拒绝靶特异性寡核苷酸探针。

8. 发明授权

US07078167B2 Arrays having background features and methods for using the same 有权
公开(公告)号：US07078167B2
公开(公告)日：2006-07-18
申请号：US09899381
申请日：2001-07-02
申请人： Glenda C. Delenstarr , Paul K. Wolber , Theodore R. Sana
发明人： Glenda C. Delenstarr , Paul K. Wolber , Theodore R. Sana
IPC分类号： C12Q1/68 , G01N33/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6837 , Y10T436/143333 , C12Q2545/107
摘要： Nucleic acid arrays that have background features, and methods for using the same, are provided. The subject nucleic acid arrays include both hybridization features and background features, where the background features provide a background signal in a hybridization assay that is made up of a feature substrate component, a nucleic acid probe component and a nucleic acid probe non-specific binding component. In practicing the subject methods, the arrays are contacted with a sample and signals are observed for both hybridization features and background features. The background feature signal is then subtracted from the hybridization feature signal to obtain a background corrected hybridization feature signal that is employed as the output of the assay, e.g., to determine the presence, either qualitatively or quantitatively, of the analyte target nucleic acid in the sample. Also provided are kits for use in practicing the subject methods.

9. 发明授权

US06403314B1 Computational method and system for predicting fragmented hybridization and for identifying potential cross-hybridization 失效
标题翻译：用于预测片段化杂交和鉴定潜在交叉杂交的计算方法和系统
公开(公告)号：US06403314B1
公开(公告)日：2002-06-11
申请号：US09498608
申请日：2000-02-04
申请人： Daniel H. Lange , Nicholas M. Sampas , Paul K. Wolber , Zohar H. Yakhini
发明人： Daniel H. Lange , Nicholas M. Sampas , Paul K. Wolber , Zohar H. Yakhini
IPC分类号： C12Q168
CPC分类号： G06F19/20 , C12Q1/6813
摘要： A computational method and system for predicting the hybridization potential for two polymers. A probe/target interaction matrix is prepared to contain indications of all possible probe/target subunit interaction stabilities. The probe/target interaction matrix is analyzed to create a list of possible single-fragment hybridizations. A graph is then generated with vertices representing fragments, and edges representing possible loops in one or both of the probe and target sequences that allow the pair of fragments interconnected by the edge to coexist within a multi-fragment cross-hybridization. Finally, the graph is analyzed to construct a list of all possible single-fragment and multi-fragment cross-hybridizations possible between the probe molecule and the target molecule. The different hybridizations are scored and sorted by score.
摘要翻译：一种用于预测两种聚合物杂交势的计算方法和系统。准备探针/靶相互作用矩阵以包含所有可能的探针/靶亚单位相互作用稳定性的指示。分析探针/靶相互作用矩阵以创建可能的单片段杂交的列表。然后，生成具有表示片段的顶点的图形，以及表示探针和靶序列中的一个或两个中的可能环的边缘，其允许边缘互连的片段在多片段交叉杂交中共存。最后，分析该图以构建探针分子和靶分子之间可能的所有可能的单片段和多片段交叉杂交的列表。不同的杂交被评分并按分数排序。

10. 发明申请

US20110263445A1 Multidentate Arrays 审中-公开
标题翻译：多数数组
公开(公告)号：US20110263445A1
公开(公告)日：2011-10-27
申请号：US12423740
申请日：2009-04-14
申请人： Paul K. Wolber
发明人： Paul K. Wolber
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C12Q1/6837 , C12Q2525/161 , C12Q2565/513 , C12Q2565/107
摘要： A method of evaluating for the presence of a target polynucleotide in a sample, using an addressable array of multiple polynucleotide probes linked to a substrate. The sample is exposed to the array and a set of polynucleotide target probes, such that target polynucleotide which may be present will bind to a predetermined feature of the array through multiple target probes of the set by forming at respective target regions on a target molecule, simultaneous hybrids with anti-target regions of the multiple target probes. A binding pattern on the array is observed and the presence of the target polynucleotide evaluated based on the observed binding pattern. Kits using such arrays, and methods for selecting target probes are further provided.
摘要翻译：使用连接到底物的多个多核苷酸探针的可寻址阵列来评估样品中靶多核苷酸的存在的方法。将样品暴露于阵列和一组多核苷酸靶探针，使得可存在的靶多核苷酸通过在靶分子上的各个靶区域形成而通过该组的多个靶探针结合阵列的预定特征，同时具有多个目标探针的抗靶区域的杂交体。观察到阵列上的结合模式，并且基于观察到的结合模式评价靶多核苷酸的存在。进一步提供使用这种阵列的套件以及用于选择目标探针的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式