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1. 发明申请

US20130052209A1 PROTEIN DRUG FORMULATIONS AND PACKAGES 审中-公开
标题翻译：蛋白质药物制剂和包装
公开(公告)号：US20130052209A1
公开(公告)日：2013-02-28
申请号：US13642333
申请日：2011-04-22
申请人： Elizabeth Murphy Topp , Frederick E. Regnier , Jun Zhang
发明人： Elizabeth Murphy Topp , Frederick E. Regnier , Jun Zhang
IPC分类号： A61K39/395 , C07K16/18
CPC分类号： A61K39/39591 , A61K47/641 , A61K47/645 , A61K2039/505 , C07K7/08 , C07K14/31 , C07K16/00 , C07K16/1282 , C07K2317/94
摘要： Compositions and methods that include stabilized protein drugs are described. In addition, protein drug formulations that are more stable under ambient conditions are described. The formulations include one or more polyamino acid ligands of the protein drug.
摘要翻译：描述了包含稳定的蛋白质药物的组合物和方法。此外，描述了在环境条件下更稳定的蛋白质药物制剂。制剂包括蛋白质药物的一种或多种聚氨基酸配体。

2. 发明授权

US4243753A Apparatus for enzyme detection 失效
标题翻译：酶检测装置
公开(公告)号：US4243753A
公开(公告)日：1981-01-06
申请号：US881577
申请日：1978-02-27
申请人： Frederick E. Regnier , Shung-Ho Chang
发明人： Frederick E. Regnier , Shung-Ho Chang
IPC分类号： C12M1/34 , C12M1/40
CPC分类号： C12M21/18 , C12M25/16 , C12M27/00 , C12M33/04 , C12M47/10 , C12M47/12 , Y10S435/803 , Y10S435/808 , Y10S435/815
摘要： An apparatus is disclosed for enzyme detection that is particularly well suited for high performance liquid chromatography. The apparatus is basically a flow-through enzyme detector that is capable of maintaining zero-order reaction kinetics with respect to substrate during enzyme detection and this results in a linear accumulation of product that can be readily measured to provide a dependable readout of detected enzymes. A sample in liquid form that may include one or more enzymes is coupled through a separation column to separate the enzymes, after which a reaction causing substrate is added and the resulting mixture is caused to flow through a reaction chamber, reaction products then being detected with the readout therefrom indicating the detected enzymes. The dimensions of the reaction chamber are dependent upon flow rates and must be selected to allow the mixture to remain in the chamber for a preselected reaction time. In addition, the chamber and packing material therein are selected to assure minimization of chromatographic profile distortion by band spreading as well as preventing separation of the components of the mixture passing therethrough. To this end, the chamber and packing material within the chamber must be of non-porous material, inert to proteins to be detected, and must assure even flow conditions.
摘要翻译：公开了用于酶检测的装置，其特别适用于高效液相色谱法。该装置基本上是一种流通酶检测器，其在酶检测期间能够保持相对于底物的零级反应动力学，并且这导致可以容易地测量以产生检测到的酶的可靠读数的产物的线性积累。可以包括一种或多种酶的液体形式的样品通过分离柱偶联以分离酶，然后加入反应引发的底物并使所得混合物流过反应室，然后用反应产物检测反应产物其读数指示检测到的酶。反应室的尺寸取决于流速，并且必须选择以允许混合物保留在室中预选的反应时间。此外，其中的室和包装材料被选择为通过带扩展确保最小化色谱分布畸变，并且防止通过其中的混合物的组分的分离。为此，腔室中的腔室和包装材料必须是无孔材料，对待检测的蛋白质是惰性的，并且必须确保均匀的流动条件。

3. 发明授权

US4245005A Pellicular coated support and method 失效
标题翻译：薄膜涂层支撑和方法
公开(公告)号：US4245005A
公开(公告)日：1981-01-13
申请号：US16031
申请日：1979-02-28
申请人： Frederick E. Regnier , Andrew J. Alpert
发明人： Frederick E. Regnier , Andrew J. Alpert
IPC分类号： B01D15/04 , B01J20/32 , B01J39/16 , B05D1/18 , C03C17/28 , C04B41/46 , C04B41/82 , B01D15/08 , B05D3/14 , G01N31/08
CPC分类号： B82Y30/00 , B01J20/0211 , B01J20/06 , B01J20/08 , B01J20/103 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28004 , B01J20/28019 , B01J20/28078 , B01J20/286 , B01J20/3092 , B01J20/3204 , B01J20/3272 , B01J20/328 , B01J20/3282 , B01J39/165 , B05D1/185 , B82Y40/00 , C03C17/28 , C04B41/009 , C04B41/46 , C04B41/82 , B01J2220/54 , B01J2220/58 , Y10T428/31536
摘要： A pellicular coated support and method for producing such a support are disclosed. The support includes a thin layer of adsorbate that is adsorbed to and cross-linked on an inorganic support material with the thus formed support being particularly well suited for use in liquid chromatography. An inorganic support of silica, alumina or titania has the pellicular coating formed thereon with the coating being an amine that is crosslinked by a crosslinking agent of epoxy resin, bromide or nitro alcohol.
摘要翻译：公开了一种用于制造这种载体的带状涂层载体和方法。载体包括被吸附并在无机载体材料上交联的被吸附的薄层，由此形成的载体特别适用于液相色谱。二氧化硅，氧化铝或二氧化钛的无机载体在其上形成有胶体涂层，涂层是通过环氧树脂，溴化物或硝基醇的交联剂交联的胺。

4. 发明授权

US3983299A Bonded carbohydrate stationary phases for chromatography 失效
标题翻译：用于色谱法的结合的碳水化合物固定相
公开(公告)号：US3983299A
公开(公告)日：1976-09-28
申请号：US537197
申请日：1974-12-30
申请人： Frederick E. Regnier
发明人： Frederick E. Regnier
IPC分类号： B01J20/281 , B01J20/32 , B05D7/24 , B32B17/02 , G01N30/93
CPC分类号： B01J20/286 , B01J20/28078 , B01J20/3204 , B01J20/3219 , B01J20/3259 , B01J20/3263 , B01J20/3274 , G01N30/93 , B01J2220/54 , B01J2220/58 , Y10T428/2995 , Y10T428/2996 , Y10T428/31612
摘要： A carbohydrate bonded support for use in a chromatographic system, and a method for preparation of such a support is disclosed herein. An inorganic support has a thin layer of carbohydrate or carbohydrate derivative covalently linked to the surface so that the thus formed support has excellent mechanical stability as well as the ability to avoid adsorbing or denaturing sensitive biological compounds, the carbohydrate bonded support thus combining the advantages of known supports without also incorporating the disadvantages thereof.
摘要翻译：本文公开了用于色谱系统的碳水化合物键合载体和制备这种载体的方法。无机载体具有与表面共价连接的碳水化合物或碳水化合物衍生物的薄层，使得如此形成的载体具有优异的机械稳定性以及避免吸附或变性敏感的生物化合物的能力，因此碳水化合物结合的载体结合了已知的支撑件，但也不包括其缺点。

5. 发明授权

US4920152A Reversed-phase packing material and method 失效
标题翻译：反相包装材料及方法
公开(公告)号：US4920152A
公开(公告)日：1990-04-24
申请号：US862750
申请日：1986-05-13
申请人： Frederick E. Regnier , William Kopaciewicz
发明人： Frederick E. Regnier , William Kopaciewicz
IPC分类号： B01J20/281 , B01D15/32 , B01J20/284 , B01J20/32 , G01N30/88
CPC分类号： B01D15/325 , B01J20/287 , B01J20/3204 , B01J20/3272 , B01J20/328 , B01J20/3282 , B01J20/3285 , B01J2220/54
摘要： Reversed-phase materials and a method for producing such materials are disclosed. In making the materials, first a thin layer of an adsorbate comprising reactive amine groups is adsorbed to a support material such as silica, alumina or titania. A portion of the reactive amine groups of said adsorbed coating are then reacted with an amount of a hydrophobic reagent sufficient to effect crosslinking of said coating. At least one remaining reactive amine group, and preferably all of the reactive amine groups of said crosslinked adsorbed coating, are then reacted, preferably in the presence of a proton scavenger, with an amount of a hydrophobic anhydride sufficient to form an amide bond with said reactive amine.
摘要翻译：公开了反相材料及其制造方法。在制造材料时，首先将包含反应性胺基的吸附物的薄层吸附到载体材料如二氧化硅，氧化铝或二氧化钛上。然后将所述吸附的涂层的一部分反应性胺基与一定量的足以实现所述涂层交联的疏水试剂反应。然后，优选在质子清除剂存在下使至少一个剩余的反应性胺基团，优选所有交联的吸附涂层的所有反应性胺基团与一定量的足够与所述的交联吸附涂层形成酰胺键的疏水酸酐反应活性胺。

6. 发明授权

US4560704A Polyamine based bonded phase chromatography 失效
标题翻译：多胺基键合相色谱法
公开(公告)号：US4560704A
公开(公告)日：1985-12-24
申请号：US441057
申请日：1982-11-12
申请人： Frederick E. Regnier , Shri P. Gupta
发明人： Frederick E. Regnier , Shri P. Gupta
IPC分类号： B01J20/32 , C08F30/08 , C08G77/04
CPC分类号： B01J20/289 , B01J20/3219 , B01J20/3257 , B01J20/3263 , B01J20/3272
摘要： Bonded phase chromatography is disclosed wherein an organosilane reacts with a polyamine to form a support matrix which is attached to the inorganic surface of a support structure. In forming an organic polyamine support matrix, a neutral organosilane is bonded to the inorganic surface with the organosilane having a functional group sufficiently reactive so that an organic amine polymer reacts with the functional group to cause covalent bonding of the organic amine polymer to the inorganic surface. By addition of an organic stationary phase group, the organic polyamine support matrix is formed into any predetermined one of a plurality of types of chromatography supports.
摘要翻译：公开了粘合相色谱法，其中有机硅烷与多胺反应以形成附着于载体结构的无机表面的载体基质。在形成有机多胺载体基质时，中性有机硅烷与无机表面结合，具有官能团的有机硅烷具有充分的反应性，使得有机胺聚合物与官能团反应，引起有机胺聚合物与无机表面的共价键合。通过加入有机固定相基团，有机多胺载体基质形成多种色谱载体中的任何预定的一种。

7. 发明授权

US4108603A Method for rapid analysis of hemoglobin variants by high speed liquid chromatography 失效
标题翻译：通过高速液相色谱快速分析血红蛋白变体的方法
公开(公告)号：US4108603A
公开(公告)日：1978-08-22
申请号：US798135
申请日：1977-05-18
申请人： Frederick E. Regnier , Shung-Ho Chang
发明人： Frederick E. Regnier , Shung-Ho Chang
IPC分类号： G01N30/96 , G01N33/72 , G01N31/08 , G01N33/16
CPC分类号： G01N33/721 , B01J20/283 , B01J20/289 , G01N30/96
摘要： A method for analysis of hemoglobin variants by high speed liquid chromatography and, more particularly, by ion-exchange chromatography. Through the use of high flow rates and pressures, the hemoglobin variants can be rapidly forced through the separation column to thus enable rapid analysis. A bonded phase inorganic support column is utilized for separation of the hemoglobin variants with the profiles thereof then being obtained. The bonded phase support preferably includes ion-exchange groups bonded through a carbohydrate intermediate to controlled porosity glass. A pair of different columns may be utilized to separate the hemoglobin variants with one of the columns being an anion-exchanger, and more particularly, a diethylaminoethanol glycophase, controlled porosity glass column, with the other column being a cation-exchanger, and more particularly, a carboxymethyl glycophase, controlled porosity glass column. The hemoglobin profiles obtained from such a pair of columns are different and the combination of chromatographic data obtained identify the particular hemoglobin variants and can thus be utilized for diagnosis purposes.
摘要翻译：用于通过高速液相色谱分析血红蛋白变体的方法，更具体地，通过离子交换色谱分析方法。通过使用高流量和压力，血红蛋白变体可以快速强制通过分离柱，从而实现快速分析。使用键合相无机载体柱用于分离血红蛋白变体，然后获得其轮廓。键合相载体优选包括通过碳水化合物中间体键合到受控多孔玻璃上的离子交换基团。可以使用一对不同的柱来分离血红蛋白变体，其中一个柱是阴离子交换剂，更具体地，二乙基氨基乙醇糖相，控制孔隙率玻璃柱，另一个柱是阳离子交换剂，更特别地，羧甲基糖相，控孔孔玻璃柱。从这样的一对柱获得的血红蛋白谱是不同的，并且所得到的色谱数据的组合识别特定的血红蛋白变体，因此可以用于诊断目的。

8. 发明授权

US08068987B2 Method and system for profiling biological systems 有权
标题翻译：生物系统分析方法和系统
公开(公告)号：US08068987B2
公开(公告)日：2011-11-29
申请号：US10218880
申请日：2002-08-13
申请人： Noubar B. Afeyan , Jan van der Greef , Frederick E. Regnier , Aram S. Adourian , Eric K. Neumann , Matej Oresic , Elwin Robbert Verheij
发明人： Noubar B. Afeyan , Jan van der Greef , Frederick E. Regnier , Aram S. Adourian , Eric K. Neumann , Matej Oresic , Elwin Robbert Verheij
IPC分类号： G01N33/48 , G01N33/50
CPC分类号： G06F19/24 , Y10T436/24
摘要： The present invention provides methods and systems for developing profiles of a biological system based on the discernment of similarities, differences, and/or correlations between biomolecular components of a plurality of biological samples. Preferably, the method comprises utilizing hierarchical multivariate analysis of spectrometric data at one or more levels of correlation.
摘要翻译：本发明提供了基于对多个生物样品的生物分子组分之间的相似性，差异和/或相关性的鉴别来开发生物系统的分布的方法和系统。优选地，该方法包括在一个或多个相关级别上利用光谱数据的分层多变量分析。

9. 发明授权

US4029583A Chromatographic supports and methods and apparatus for preparing the same 失效
标题翻译：色谱支架及其制备方法和装置
公开(公告)号：US4029583A
公开(公告)日：1977-06-14
申请号：US554071
申请日：1975-02-28
申请人： Shung Ho Chang , Frederick E. Regnier
发明人： Shung Ho Chang , Frederick E. Regnier
IPC分类号： B01J20/32 , C12N11/14 , B01D39/00 , B01D39/14
CPC分类号： B01J20/3257 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3272 , C12N11/14
摘要： Chromatographic supports are disclosed as are methods and apparatus for preparing the same. The support includes an inorganic support matrix that is capable of reacting with silanols, a stationary phase, and an intermediate silane coupling agent having a silicon functional group capable of bonding to the support surface and an organic functional group capable of bonding to the stationary phase. The inorganic support matrix is preferably controlled porosity glass or silica while the coupling agent may include a simple carbohydrate, a carbohydrate derivative, or a polymer to which the stationary phase is attached. A plurality of chromatographic supports and stationary phases are disclosed and two methods of bonding of stationary phases to supports are also disclosed. In addition, apparatus including a fluidized bed is also disclosed to prepare supports.
摘要翻译：公开了色谱载体的制备方法和装置。载体包括能够与硅烷醇，固定相和具有能够与载体表面键合的硅官能团的中间体硅烷偶联剂和能够键合到固定相的有机官能团的硅烷偶联剂的无机载体基质。无机载体基质优选为多孔玻璃或二氧化硅，而偶联剂可包括简单的碳水化合物，碳水化合物衍生物或固定相附着的聚合物。公开了多个色谱载体和固定相，并且还公开了将固定相结合到载体上的两种方法。此外，还公开了包括流化床的装置以制备载体。

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