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1. 发明授权

US5853993A Signal enhancement method and kit 失效
标题翻译：信号增强方法和试剂盒
公开(公告)号：US5853993A
公开(公告)日：1998-12-29
申请号：US735381
申请日：1996-10-21
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region, whereby the hybridization of multiple reporter probes to the homopolymeric region provides for signal amplification; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.
摘要翻译：本发明公开了一种用于检测具有均聚区和靶序列的靶核酸分析物的信号放大方法。该方法包括（a）在杂交条件下将分析物与多种报道探针接触，每个探针包括信号区和寡核苷酸序列，其与分析物均聚区相互补充并能够形成稳定的杂交，由此杂交的多个报道探针到均聚区域提供信号放大; 和（b）通过在杂交条件下使分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。

2. 发明授权

US6110682A Signal enhancement method and kit 失效
标题翻译：信号增强方法和试剂盒
公开(公告)号：US6110682A
公开(公告)日：2000-08-29
申请号：US201674
申请日：1998-11-30
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.
摘要翻译：本发明公开了一种用于检测具有均聚区和靶序列的靶核酸分析物的信号放大方法。该方法包括（a）在杂交条件下将分析物与多个报道探针接触，每个探针包括与分析物均聚区互补并能够与分析物均聚区形成稳定杂交的信号区和寡核苷酸序列; 和（b）通过在杂交条件下使分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。

3. 发明授权

US6103474A Hybridization assay signal enhancement 失效
标题翻译：杂交测定信号增强
公开(公告)号：US6103474A
公开(公告)日：2000-08-15
申请号：US183619
申请日：1998-10-30
申请人： Douglas J. Dellinger , SueAnn C. Dahm , Diane D. Ilsley , Robert A. Ach , Mark A. Troll
发明人： Douglas J. Dellinger , SueAnn C. Dahm , Diane D. Ilsley , Robert A. Ach , Mark A. Troll
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/682
摘要： A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple helix structures for further signal enhancement. Multiply-labeled hairpin reporter probes may be employed according to the invention. Also, a kit for carrying out the invention includes one or more capture probes immobilized on a surface, reporter probes each having a signal region and a sequence for binding analyte homopolymer regions, and, optionally, reagents for addition of homopolymer regions to nucleic acid analytes and for carrying out the hybridization reaction.
摘要翻译：用于检测具有均聚区和靶序列的靶核酸分析物的信号扩增方法包括以下步骤：在杂交条件下与多个报道探针接触分析物，每个报道探针包括信号区和互补的寡核苷酸序列并且能够与分析物均聚区形成稳定的杂交体以形成分析物：报道探针杂交体; 并通过在杂交条件下将分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。在将分析物靶序列与捕获探针接触之前可以形成分析物：报告物探针杂交体，因此将分析物靶序列与捕获探针接触的结果导致分析物的形成：报道探针：捕获探针复合物。分析物：捕获探针杂交体可以以阵列形式固定在固体大体上平坦的表面上。多个报道探针可以形成用于进一步信号增强的三重螺旋结构。根据本发明，可以使用多重标记的发夹报道探针。此外，用于实施本发明的试剂盒包括固定在表面上的一种或多种捕获探针，各自具有信号区域的报道探针和用于结合分析物均聚物区域的序列，以及任选的用于向核酸分析物加入均聚物区域的试剂并用于进行杂交反应。

4. 发明授权

US09273086B2 Protected monomer and method of final deprotection for RNA synthesis 有权
标题翻译：受保护的单体和RNA合成的最终脱保护方法
公开(公告)号：US09273086B2
公开(公告)日：2016-03-01
申请号：US13531191
申请日：2012-06-22
申请人： Douglas J. Dellinger , Joel Myerson , Agnieszka B. Sierzchala , Geraldine F. Dellinger , Zoltan Timar
发明人： Douglas J. Dellinger , Joel Myerson , Agnieszka B. Sierzchala , Geraldine F. Dellinger , Zoltan Timar
IPC分类号： C07H21/02 , C07H13/12 , C07H19/067 , A61K31/7125 , A61K31/712 , C07H19/00 , C07H19/167 , C07H21/00 , C07H23/00
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2′-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2′-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.
摘要翻译：使固体支持物结合的多核苷酸去保护的方法包括在足以使2'-保护的核糖核苷酸残基脱保护的条件下使多核苷酸与包含二胺的组合物接触的步骤。固体支持结合的多核苷酸具有至少一个2'-保护的核糖核苷酸残基，其具有以下结构：其中BP是被保护或未被保护的杂环; R 12是选自烃基，取代的烃基，芳基和取代的芳基的保护基; X是O或S; PG是硫代氨基甲酸酯保护基。

5. 发明申请

US20120238737A1 THIOCARBON-PROTECTING GROUPS FOR RNA SYNTHESIS 有权
标题翻译：用于RNA合成的THIOCARBON保护组
公开(公告)号：US20120238737A1
公开(公告)日：2012-09-20
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
IPC分类号： C07H1/00 , C07H19/20 , C07H21/02 , C07H19/10
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译：本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。

6. 发明申请

US20100331533A1 Use Of Thioacetic Acid Derivatives In The Sulfurization Of Oligonucleotides With Phenylacetyl Disulfide 有权
标题翻译：硫代乙酸衍生物在使用苯乙酰基二硫化物的寡核苷酸硫化中的应用
公开(公告)号：US20100331533A1
公开(公告)日：2010-12-30
申请号：US12494493
申请日：2009-06-30
申请人： Agnieszka B. Sierzchala , Douglas J. Dellinger , Victor R. Mokler , Zoltan Timar
发明人： Agnieszka B. Sierzchala , Douglas J. Dellinger , Victor R. Mokler , Zoltan Timar
IPC分类号： C07H21/00 , C09K3/00
CPC分类号： C07H21/02
摘要： A method and compositions for sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide. The methods employ a phenylacetyl disulfide reagent (known as PADS), phenylthioacetic acid (PTAA) in the presence or absence or N-alkyl imidazole in industrially preferred solvents or solvents that are derived from renewable resources. The use of PTAA eliminates the need to “age” the PADS solution prior to its use in sulfurization reactions.
摘要翻译：一种在寡核苷酸中硫化至少一种亚磷酸酯或硫代亚磷酸酯键的方法和组合物。该方法在工业上优选的可再生资源溶剂或溶剂中，在存在或不存在或N-烷基咪唑的情况下使用苯乙酰二硫化物（称为PADS），苯硫基乙酸（PTAA）。使用PTAA消除了在使用硫化反应之前“PADS”溶液的“老化”的需要。

7. 发明申请

US20090082554A1 Thiocarbonate linkers for polynucleotides 有权
标题翻译：多核苷酸的硫代碳酸酯接头
公开(公告)号：US20090082554A1
公开(公告)日：2009-03-26
申请号：US11903821
申请日：2007-09-24
申请人： Douglas J. Dellinger , Zoltan Timar , Joel Myerson , Geraldine Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Joel Myerson , Geraldine Dellinger , Marvin H. Caruthers
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C07H21/02 , C07H21/04
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译：在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。

8. 发明授权

US07427679B2 Precursors for two-step polynucleotide synthesis 失效
标题翻译：两步多核苷酸合成的前体
公开(公告)号：US07427679B2
公开(公告)日：2008-09-23
申请号：US10652048
申请日：2003-08-30
申请人： Douglas J. Dellinger , Agnieszka B. Sierzchala , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Agnieszka B. Sierzchala , Marvin H. Caruthers
IPC分类号： C07H19/10 , C07H19/20
CPC分类号： C07H21/00 , C07H19/00 , Y02P20/55
摘要： Precursors for use in the synthesis of polynucleotides are disclosed. The precursors include a heterocyclic base having an exocyclic amine group and a substituted or unsubstituted triaryl methyl protecting group bound to the exocyclic amine group. In particular embodiments, the precursor has the structure: wherein: O and H represent oxygen and hydrogen, respectively, R1 is hydrido, hydroxyl, protected hydroxyl, lower alkyl, modified lower alkyl, or alkoxy, one of R2 or R3 is a hydroxyl protecting group; and the other of R2 or R3 is a reactive group capable of reacting with a reactive site hydroxyl, Base is a heterocyclic base having an exocyclic amine group, and Tram is a triaryl methyl group having the structure (V) wherein the broken line represents a bond to the amino nitrogen of the exocyclic amine group, and R4, R5 and R6 are independently selected from unsubstituted or substituted aryl groups, provided that at least one of R4, R5, and R6 is an aryl group other than phenyl and other than substituted phenyl.
摘要翻译：公开了用于合成多核苷酸的前体。前体包括具有环外胺基的杂环碱基和与环外胺基结合的取代或未取代的三芳基甲基保护基。在具体实施方案中，前体具有以下结构：其中：O和H分别表示氧和氢，R 1是氢，羟基，被保护的羟基，低级烷基，经修饰的低级烷基或烷氧基，R 2或R 3之一是羟基保护基; R 2或R 3中的另一个为可与反应性位点羟基反应的反应性基团，碱基为具有环外胺基的杂环碱基，Tram为具有结构（V）的三芳基甲基，其中虚线表示与环外胺基团的氨基氮的键，并且R 4，R 5和R 6独立地选自未取代或取代的芳基，条件是R 4，R 5和R 6中的至少一个是芳基除苯基以外的基团，而不是取代的苯基。

9. 发明申请

US20080206850A1 Methods and compositions for DNA synthesis 审中-公开
标题翻译： DNA合成的方法和组合物
公开(公告)号：US20080206850A1
公开(公告)日：2008-08-28
申请号：US11713221
申请日：2007-02-28
申请人： Douglas J. Dellinger , Geraldine F. Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Geraldine F. Dellinger , Marvin H. Caruthers
IPC分类号： C07H21/04 , C12M1/34
CPC分类号： C07H21/04 , Y02P20/55
摘要： In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful of the synthesis of DNA, and in particular for the synthesis of long sequences of DNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step DNA synthesis, that involves the use of peroxyanions in combination with fluoride anions.
摘要翻译：在一些实施方案中，本公开涉及新的亚磷酰胺组合物，其具有含有甲硅烷基的碳酸酯或硫代碳酸酯或醚作为可用于合成DNA的5'-羟基保护基团，特别是用于合成长序列DNA（例如，> 50 mer）。在一些实施方案中，提供了同时氧化核苷磷酸三酯键并除去5'-羟基保护基团的方法，使该方法成为新的2-步DNA合成，其涉及使用过氧阴离子与氟化物阴离子

10. 发明申请

US20080146787A1 Thioether substituted aryl carbonate protecting groups 有权
标题翻译：硫醚取代的芳基碳酸酯保护基
公开(公告)号：US20080146787A1
公开(公告)日：2008-06-19
申请号：US11897898
申请日：2007-08-31
申请人： Zoltan Timar , Zoltan Kupihar , Douglas J. Dellinger , Marvin H. Caruthers
发明人： Zoltan Timar , Zoltan Kupihar , Douglas J. Dellinger , Marvin H. Caruthers
IPC分类号： C07H21/00 , C07H19/04 , C07H21/04 , C07H21/02
CPC分类号： C07H19/04 , C07H21/00 , C07H21/02 , C07H21/04 , Y02P20/55
摘要： Embodiments of the invention include thioether substituted aryl carbonate protecting groups, and nucleoside monomers protected with thioether substituted aryl carbonate protecting groups. Aspects of the invention further included methods of synthesizing nucleic acids, e.g., oligonucleotides, using such protected nucleoside monomer monomers, as well as nucleic acids produced using methods of the invention and compositions thereof.
摘要翻译：本发明的实施方案包括硫醚取代的芳基碳酸酯保护基，和用硫醚取代的芳基碳酸酯保护基保护的核苷单体。本发明的方面还包括使用这种受保护的核苷单体/单体合成核酸例如寡核苷酸的方法，以及使用本发明的方法和其组合物制备的核酸。

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