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1. 发明授权

US4631408A Method of simultaneously determining gauge and orientation of polymer films 失效
标题翻译：同时测定聚合物膜的规格和取向的方法
公开(公告)号：US4631408A
公开(公告)日：1986-12-23
申请号：US653962
申请日：1984-09-24
申请人： David Zelmanovic , Stanley J. Kishner
发明人： David Zelmanovic , Stanley J. Kishner
IPC分类号： G01N21/27 , G01B11/06 , G01N21/31 , G01N21/35 , G01N21/86 , G01N33/44 , G01J1/00
CPC分类号： G01N33/442 , G01N21/3563 , G01N21/86 , G01N2021/3174 , G01N2021/869
摘要： In the manufacture of a multilayer polymer film, the thickness and degree of orientation are simultaneously measured. A beam of infrared radiation having predetermined wavelengths is either transmitted through or reflected by the polymer film to be measured and received by a photodetector. The photodetector provides an electrical output signal which can be analyzed to determine the thickness and degree of orientation.
摘要翻译：在多层聚合物膜的制造中，同时测量厚度和取向度。具有预定波长的红外辐射束或者透射通过或被被测量的聚合物膜反射并由光电检测器接收。光电检测器提供电输出信号，其可以被分析以确定取向的厚度和程度。

2. 发明授权

US4677298A Method of monitoring ink-water balance on a lithographic printing press 失效
标题翻译：在平版印刷机上监测墨水平衡的方法
公开(公告)号：US4677298A
公开(公告)日：1987-06-30
申请号：US618252
申请日：1984-06-07
申请人： David Zelmanovic , Stanley J. Kishner
发明人： David Zelmanovic , Stanley J. Kishner
IPC分类号： B41F33/00 , G01N21/47 , G01N21/49 , G01N21/55
CPC分类号： G01N21/4738 , B41F33/0054 , Y10S101/45
摘要： In a lithographic printing system, a light source and a plurality of photodetectors are used to monitor the amount of fountain solution interacting with the ink. The light source irradiates an ink form roller and the photodetectors are disposed at a specular angle and at one or more non-specular angles to monitor the reflected light. An electronic circuit is responsive to the output of the photodetectors and is used for providing an indication of the amount of fountain solution emulsified in the ink, and lying on the surface of the ink.
摘要翻译：在平版印刷系统中，使用光源和多个光电检测器来监测与油墨相互作用的润版液的量。光源照射油墨辊，并且光电探测器以镜面角度和一个或多个非镜面角度设置以监测反射光。电子电路响应于光电检测器的输出，并且用于提供在油墨中乳化的并且位于油墨表面上的润版液的量的指示。

3. 发明授权

US06521729B1 Monodisperse fluorine-containing synthetic polymer particles having a defined refractive index for use as standards and calibrators in flow cytometry 失效
$Monodisperse fluorine-containing synthetic polymer particles having a defined refractive index for use as standards and calibrators in flow cytometry$
标题翻译：具有限定折射率的单分散含氟合成聚合物颗粒用作流式细胞术中的标准物和校准物
公开(公告)号：US06521729B1
公开(公告)日：2003-02-18
申请号：US09432905
申请日：1999-11-02
申请人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
发明人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
IPC分类号： C08F12024
CPC分类号： G01N15/1012 , G01N2015/1062 , G01N2015/1486 , G01N2015/149 , Y10T436/10 , Y10T436/101666
摘要： The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
摘要翻译：本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法，其提供了在这些仪器中使用的优点。在校准方法中使用的聚合物颗粒或珠粒是球形的，平均粒径为约1至约8微米，相对较窄的颗粒分布和低折射率，即约1.35至约1.45。颗粒安全，长时间稳定，特别适合用作血液分析仪校准物质。本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。特别地，使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化，用于随后准确可靠地测定全血样品中的红细胞，网织红细胞，白细胞和血小板。

4. 发明申请

US20050026240A1 Reagent composition for the analysis of residual white blood cells in leuko-reduced blood banking products 有权
标题翻译：用于分析白血病减少血库产品中残留白细胞的试剂组合物
公开(公告)号：US20050026240A1
公开(公告)日：2005-02-03
申请号：US10896338
申请日：2004-07-21
申请人： Michael Sommer , Pasquale Degiorgio , Bronislaw Czech , Gena Fischer , E. Chapman , David Zelmanovic , Jolanta Kunicka
发明人： Michael Sommer , Pasquale Degiorgio , Bronislaw Czech , Gena Fischer , E. Chapman , David Zelmanovic , Jolanta Kunicka
IPC分类号： G01N33/49 , G01N1/30 , G01N15/14 , G01N33/48 , G01N33/50
CPC分类号： G01N33/5002 , G01N2015/1486 , G01N2015/1488 , Y10T436/101666 , Y10T436/107497
摘要： The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.
摘要翻译：通过用足够量的裂解试剂形成白细胞减少的血液制品的悬浮液来进行白细胞减少的血液制品样品中的残余白细胞的计数和分析。裂解试剂包含具有低摩尔浓度的缓冲液和非离子表面活性剂。将白细胞减少的血液制品和裂解试剂的悬浮液在足以在不损伤白细胞的情况下足以选择性地裂解血小板和红细胞的温度下孵育足够的时间。然后将裂解的血液制品的白细胞与合适的染料接触以染色白细胞，并测量染色的白细胞数。裂解试剂不含苛刻的有机溶剂，可能会损坏自动化临床分析仪的塑料部件。

5. 发明授权

US6114173A Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood 失效
标题翻译：全自动化方法和试剂组合物，用于快速鉴定和表征全血中的网织红细胞和血小板
公开(公告)号：US6114173A
公开(公告)日：2000-09-05
申请号：US833033
申请日：1997-04-03
申请人： David Zelmanovic , Lynn Paseltiner , Martin Sorette
发明人： David Zelmanovic , Lynn Paseltiner , Martin Sorette
IPC分类号： G01N33/48 , G01N15/14 , G01N21/47 , G01N33/49 , G01N33/50 , G01N33/52 , G01N33/58
CPC分类号： G01N15/1459 , G01N33/5094 , G01N33/583 , G01N2015/0073 , G01N2015/0076 , G01N2015/0084 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： The invention provides a very fast and reliable method and reagent composition for determining reticulocyte and erythrocyte counts, identification and characterization, as well as platelet counts, in a whole blood sample using fully automated hematology analyzers. The reagent composition includes a zwitterionic surfactant as sphering agent for reticulocytes and erythrocytes, an organic cationic dye, e.g., Oxazine 750, for staining the reticulocytes, and buffer solution components for maintaining a reagent pH of about 7.2 to 7.8, and an osmolarity of about 292.+-.5. On the basis of the particular pH, ionic strength, and dye concentration, the reagent composition of the present method improves upon previous methods, thus allowing fully automated blood sample analyses to be completed in less than about 30 seconds, with only about a 20 second incubation of sample in the reagent solution before analysis. With such a rapid incubation time, the reagent mixture is passed through the sensing region of a flow cytometer, and the light scattered and absorbed by each cell is rapidly measured to provide accurate cell determinations and counts. The volume and hemoglobin concentration and content of each erythrocyte or reticulocyte are determined. Mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.
摘要翻译：本发明提供了使用全自动血液分析仪在全血样品中测定网织红细胞和红细胞计数，鉴定和表征以及血小板计数的非常快速和可靠的方法和试剂组合物。试剂组合物包括两性离子表面活性剂作为网织红细胞和红细胞的球形剂，有机阳离子染料，例如用于染色网织红细胞的恶嗪750，以及用于维持试剂pH约7.2至7.8的缓冲溶液组分，以及约 292 +/- 5。基于特定的pH，离子强度和染料浓度，本方法的试剂组合物改进了先前的方法，从而允许完全自动化的血液样品分析在少于约30秒内完成，仅约20秒分析前将样品在试剂溶液中孵育。通过这样快速的孵育时间，试剂混合物通过流式细胞仪的感测区域，并且快速测量每个细胞散射和吸收的光，以提供精确的细胞测定和计数。确定每种红细胞或网织红细胞的体积和血红蛋白浓度和含量。从测定的细胞间细胞体积和血红蛋白浓度计算网织红细胞和/或红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白。

6. 发明授权

US6074879A Synthetic polymer particles for use as standards and calibrators in flow cytometry 失效
标题翻译：合成聚合物颗粒，用作流式细胞仪中的标准品和校准品
公开(公告)号：US6074879A
公开(公告)日：2000-06-13
申请号：US099233
申请日：1998-06-17
申请人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
发明人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
IPC分类号： G01N33/49 , G01N15/00 , G01N15/10 , G01N15/14 , G01N31/00
CPC分类号： G01N15/1012 , G01N2015/1062 , G01N2015/1486 , G01N2015/149 , Y10T436/10 , Y10T436/101666
摘要： The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
摘要翻译：本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法，其提供了在这些仪器中使用的优点。在校准方法中使用的聚合物颗粒或珠粒是球形的，平均粒径为约1至约8微米，相对较窄的颗粒分布和低折射率，即约1.35至约1.45。颗粒安全，长时间稳定，特别适合用作血液分析仪校准物质。本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。特别地，使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化，用于随后准确可靠地测定全血样品中的红细胞，网织红细胞，白细胞和血小板。

7. 发明授权

US5817519A Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples 失效
标题翻译：用于鉴定和定量血小板以及使用全血样品测定血小板激活状态的自动化方法和装置
公开(公告)号：US5817519A
公开(公告)日：1998-10-06
申请号：US742889
申请日：1996-11-01
申请人： David Zelmanovic , Gregory M. Colella , Edward J. Hetherington , Evelyn Sabrinah Chapman , Lynn Paseltiner
发明人： David Zelmanovic , Gregory M. Colella , Edward J. Hetherington , Evelyn Sabrinah Chapman , Lynn Paseltiner
IPC分类号： G01N33/483 , G01N15/14 , G01N21/49 , G01N33/49 , G01N33/48
CPC分类号： G01N15/1429 , G01N15/1209 , G01N15/12 , G01N2015/0073 , G01N2015/0084 , G01N2015/1402 , G01N2035/1032 , G01N35/1095
摘要： The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do unactivated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.
摘要翻译：本发明提供了一种用于使用自动血液仪器鉴别和定量全血样品中血小板的高灵敏度和准确的方法和系统。本发明的方法和系统提供正常血液样品和异常血液样品（例如来自血小板减少症患者）的血小板干质量和血小板成分浓度的准确测量。血小板干质量和血小板成分浓度的测定可用于评估血小板的活化状态，因为活性血小板具有比未激活的血小板具有可测量较低的组分浓度和折射指数。本发明的方法和系统还允许临床医师或熟练医师根据血小板成分浓度的测量参数确定血液样本的年龄。

8. 发明授权

US07745219B2 Reagent composition for the analysis of residual white blood cells in leuko-reduced blood banking products 有权
标题翻译：用于分析白血病减少血库产品中残留白细胞的试剂组合物
公开(公告)号：US07745219B2
公开(公告)日：2010-06-29
申请号：US10896338
申请日：2004-07-21
申请人： Michael J. Sommer , Pasquale Degiorgio , Bronislaw P. Czech , Gena Fischer , E. Sabrinah Chapman , David Zelmanovic , Jolanta Kunicka
发明人： Michael J. Sommer , Pasquale Degiorgio , Bronislaw P. Czech , Gena Fischer , E. Sabrinah Chapman , David Zelmanovic , Jolanta Kunicka
IPC分类号： G01N33/48 , G01N33/49
CPC分类号： G01N33/5002 , G01N2015/1486 , G01N2015/1488 , Y10T436/101666 , Y10T436/107497
摘要： The enumeration and analysis of residual white blood cells in a sample of leukocyte-reduced blood products is conducted by forming a suspension of the leukocyte-reduced blood products with a sufficient amount of a lysing reagent. The lysing reagent comprises a buffer with a low molar concentration, and a non-ionic surfactant. The suspension of leukocyte-reduced blood products and the lysing reagent is incubated for a sufficient time at a temperature sufficient to selectively lyse the platelets and red blood cells without damaging the white blood cells. The white blood cells of the lysed blood products are then contacted with a suitable dye to stain the white blood cells and the number of stained white blood cells is measured. The lysing reagent is free of harsh organic solvents which can damage the plastic components of automated clinical analyzers.
摘要翻译：通过用足够量的裂解试剂形成白细胞减少的血液制品的悬浮液来进行白细胞减少的血液制品样品中的残余白细胞的计数和分析。裂解试剂包含具有低摩尔浓度的缓冲液和非离子表面活性剂。将白细胞减少的血液制品和裂解试剂的悬浮液在足以在不损伤白细胞的情况下足以选择性地裂解血小板和红细胞的温度下孵育足够的时间。然后将裂解的血液制品的白细胞与合适的染料接触以染色白细胞，并测量染色的白细胞数。裂解试剂不含苛刻的有机溶剂，可能会损坏自动化临床分析仪的塑料部件。

9. 发明授权

US07670798B2 Automated method and reagent therefor assaying body fluid samples such as cerebrospinal fluid (CSF) 有权
标题翻译：自动化方法和试剂用于测定体液样品如脑脊液（CSF）
公开(公告)号：US07670798B2
公开(公告)日：2010-03-02
申请号：US10442706
申请日：2003-05-20
申请人： Leonard Ornstein , Gena Fischer , David Zelmanovic , Pamela Elsins , Jolanta Kunicka , Michael J. Malin
发明人： Leonard Ornstein , Gena Fischer , David Zelmanovic , Pamela Elsins , Jolanta Kunicka , Michael J. Malin
IPC分类号： G01N1/00 , G01N7/00 , G01N31/00 , G01N33/48 , G01N33/546
CPC分类号： G01N33/96 , A01N1/00 , G01N15/1459 , G01N2015/0073 , G01N2015/1486 , Y10T436/10 , Y10T436/11 , Y10T436/2525
摘要： The present invention describes semi- and fully-automated methods and reagents therefor for the assay and analysis of body fluid samples, particularly non-blood samples. The methods and reagents are especially useful for the assay and analysis of cerebrospinal fluid (CSF) samples. The reagent compositions sphere and fix all cells in the sample in suspension. Reported results can include red blood cell (RBC) and white blood cell (WBC) counts, WBC differential values, cell-by-cell volumes and dry-mass concentrations.
摘要翻译：本发明描述了用于体液样品，特别是非血液样品的测定和分析的半自动和全自动化方法和试剂。方法和试剂对于脑脊液（CSF）样品的测定和分析特别有用。试剂组合物球体并将悬浮液中的样品中的所有细胞固定。报告的结果可以包括红细胞（RBC）和白血球（WBC）计数，WBC差异值，逐个细胞体积和干质量浓度。

10. 发明授权

US06524858B1 Single channel, single dilution detection method for the identification and quantification of blood cells and platelets in a whole blood sample using an automated hematology analyzer 有权
标题翻译：单通道单次稀释检测方法，用于使用自动血液分析仪鉴定和定量全血样品中的血细胞和血小板
公开(公告)号：US06524858B1
公开(公告)日：2003-02-25
申请号：US09539568
申请日：2000-03-31
申请人： David Zelmanovic , Valentine Jones
发明人： David Zelmanovic , Valentine Jones
IPC分类号： G01N3348
CPC分类号： G01N33/5094 , G01N15/14 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： The invention provides a new single channel, single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel, rather than multiple channels, of an automated hematology analyzer utilizing flow cytometry and the detection of the light scattered and absorbed by each cell. The single channel utilized in the method was previously known and used only for red blood cell and reticulocyte analysis. The method involves the use of an organic dye in the reagent solution for staining the nucleic acid of reticulocytes, including reticulated platelets, and white blood cells in the sample. The single channel method developed and described is particularly useful for determining white blood cell counts and assessing parameters of a whole blood sample, for blood samples from both human and non-human mammals. The single channel method of the invention employs only one blood diluent reagent composition that is mixed with the blood sample aliquot for analysis and, optimally, a sheath/rinse reagent, thus making it simpler and easier to use. In addition, the reagent compositions of the method are more benign to the white blood cells than previous reagents, which contained various lytic surfactants for removing red blood cells, for example. The apparatus for performing the method provides an economical, streamlined and space-saving analyzer for carrying out complete blood cell analysis on a mammalian whole blood sample.
摘要翻译：本发明提供了一种新的单通道单次稀释方法和系统，用于利用流式细胞术和光散射检测的自动血液分析仪，使用单通道而不是多个通道来识别，分析和定量全血细胞成分并被每个细胞吸收。以前已知该方法中使用的单一通道，仅用于红细胞和网织红细胞分析。该方法包括在试剂溶液中使用有机染料染色网织红细胞（包括网状血小板）和样品中的白细胞的核酸。开发和描述的单通道方法对于确定来自人和非人哺乳动物的血液样品的白细胞计数和评估全血样品的参数特别有用。本发明的单通道方法仅使用一种血液稀释剂试剂组合物，其与血液样品等分试样混合以用于分析，并且最佳地使用护套/漂洗剂，从而使其更简单和更容易使用。此外，该方法的试剂组合物比以前的试剂更为良好，其含有各种用于除去红细胞的溶解表面活性剂。用于执行该方法的装置提供了一种经济，流线型和节省空间的分析仪，用于对哺乳动物全血样品进行完整的血细胞分析。

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