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1. 发明申请

US20050164404A1 Diagnostic testing process 有权
标题翻译：诊断测试过程
公开(公告)号：US20050164404A1
公开(公告)日：2005-07-28
申请号：US10497925
申请日：2002-12-12
申请人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
发明人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
IPC分类号： G01N33/52 , B01L3/00 , G01N33/48 , G01N33/543 , G01N33/545 , G01N33/553 , G01N33/558
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/807 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为结合胶体金或荧光标记的一种或多种抗体）已知结合特定蛋白质可以结合在一起所需的时间段。此预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

2. 发明申请

US20070190667A1 Diagnostic testing process and apparatus 审中-公开
标题翻译：诊断测试过程和设备
公开(公告)号：US20070190667A1
公开(公告)日：2007-08-16
申请号：US11787523
申请日：2007-04-17
申请人： Robert Cole , Andrew Sloane , William Hunter
发明人： Robert Cole , Andrew Sloane , William Hunter
IPC分类号： G01N33/543
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为与胶体金或荧光标签结合的抗体）已知结合特定蛋白质的蛋白质可以结合在一起所需的时间段。该预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

3. 发明申请

US20050124077A1 Diagnostic testing process and apparatus 有权
标题翻译：诊断测试过程和设备
公开(公告)号：US20050124077A1
公开(公告)日：2005-06-09
申请号：US10487052
申请日：2002-08-20
申请人： Robert Cole , Andrew Sloane , William Hunter
发明人： Robert Cole , Andrew Sloane , William Hunter
IPC分类号： B01L3/00 , G01N33/543
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为与胶体金或荧光标签结合的抗体）已知结合特定蛋白质的蛋白质可以结合在一起所需的时间段。该预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

4. 发明申请

US20070178541A1 Method of isolating a protein 审中-公开
标题翻译：分离蛋白质的方法
公开(公告)号：US20070178541A1
公开(公告)日：2007-08-02
申请号：US10562132
申请日：2004-06-28
申请人： Susanne Pedersen , Robert Cole , Ron Weinberger , Andrew Sloane
发明人： Susanne Pedersen , Robert Cole , Ron Weinberger , Andrew Sloane
IPC分类号： C12Q1/70 , G01N33/569 , G01N33/554 , G01N33/543
CPC分类号： G01N33/6854 , G01N33/564 , G01N33/569
摘要： The present invention provides a method for isolating and/or identifying an immunogenic protein from a protein complex comprising an immunoglobulin or a mixture thereof or an immunoglobulin-containing fraction.
摘要翻译：本发明提供了用于从包含免疫球蛋白或其混合物或含免疫球蛋白的级分的蛋白质复合物中分离和/或鉴定免疫原性蛋白的方法。

5. 发明申请

US20050019942A1 Array-based biomolecule analysis 审中-公开
标题翻译：基于阵列的生物分子分析
公开(公告)号：US20050019942A1
公开(公告)日：2005-01-27
申请号：US10865678
申请日：2004-06-10
申请人： Andrew Sloane , Malcolm Pluskal , Andrew Gooley , Janice Joss , Russell Ludowyke , Michael Hsu
发明人： Andrew Sloane , Malcolm Pluskal , Andrew Gooley , Janice Joss , Russell Ludowyke , Michael Hsu
IPC分类号： B01L3/00 , G01N27/447 , G01N35/00 , G01N35/02 , G01N21/00 , G01N1/00
CPC分类号： B01L3/5085 , G01N27/44717 , G01N27/44726 , G01N35/00029 , G01N35/028 , Y10T436/25
摘要： Separation of macromolecules by one-dimensional or two-dimensional methods, such as gel electrophoresis, produces an array of macromolecules, which can be transferred to a support, thereby producing the same array as on the gel. In the case of one-dimensional gel electrophoresis, because of the regular spacing of the gel lanes and the predictable direction of migration of the macromolecules, the positions of the macromolecule spots or bands in the array can be predicted to be at least within the area of the support corresponding to the lanes of the gel. Where the molecular weight of a macromolecule of interest is known, molecular weight markers can be used to determine where the macromolecule band is on the support, even if the macromolecule is not stained in the gel or on the support. Assays that reveal characteristics of the macromolecule can be carried out by spotting reagents onto the support in a series of microspots of small volume in a line which intersects the macromolecule band, and which corresponds to the line of the direction of migration of the macromolecules on the gel. Appropriate detection methods can be applied, depending on the reagent, to see the results. The steps for locating the bands of macromolecules, applying reagents, and detecting the effect of the reagent on the macromolecule can be automated in an appropriate instrument.
摘要翻译：通过一维或二维方法（例如凝胶电泳）分离大分子产生大分子阵列，其可以转移到载体上，从而产生与凝胶上相同的阵列。在一维凝胶电泳的情况下，由于凝胶泳道的规则间隔和大分子的可预测的迁移方向，阵列中的大分子斑点或条带的位置可以预测至少在该区域内的对应于凝胶泳道的支撑物。当感兴趣的大分子的分子量是已知的时，分子量标记可用于确定大分子带在载体上的位置，即使大分子没有在凝胶或载体上染色。可以通过将试剂点在与大分子带相交的一行中的小体积的一系列小体积的载体上，将大分子的迁移方向对应于大分子的迁移方向凝胶。可以根据试剂适用检测方法来查看结果。用于定位大分子带，应用试剂和检测试剂对大分子的影响的步骤可以在合适的仪器中自动化。

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