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1. 发明申请

US20050019942A1 Array-based biomolecule analysis 审中-公开
标题翻译：基于阵列的生物分子分析
公开(公告)号：US20050019942A1
公开(公告)日：2005-01-27
申请号：US10865678
申请日：2004-06-10
申请人： Andrew Sloane , Malcolm Pluskal , Andrew Gooley , Janice Joss , Russell Ludowyke , Michael Hsu
发明人： Andrew Sloane , Malcolm Pluskal , Andrew Gooley , Janice Joss , Russell Ludowyke , Michael Hsu
IPC分类号： B01L3/00 , G01N27/447 , G01N35/00 , G01N35/02 , G01N21/00 , G01N1/00
CPC分类号： B01L3/5085 , G01N27/44717 , G01N27/44726 , G01N35/00029 , G01N35/028 , Y10T436/25
摘要： Separation of macromolecules by one-dimensional or two-dimensional methods, such as gel electrophoresis, produces an array of macromolecules, which can be transferred to a support, thereby producing the same array as on the gel. In the case of one-dimensional gel electrophoresis, because of the regular spacing of the gel lanes and the predictable direction of migration of the macromolecules, the positions of the macromolecule spots or bands in the array can be predicted to be at least within the area of the support corresponding to the lanes of the gel. Where the molecular weight of a macromolecule of interest is known, molecular weight markers can be used to determine where the macromolecule band is on the support, even if the macromolecule is not stained in the gel or on the support. Assays that reveal characteristics of the macromolecule can be carried out by spotting reagents onto the support in a series of microspots of small volume in a line which intersects the macromolecule band, and which corresponds to the line of the direction of migration of the macromolecules on the gel. Appropriate detection methods can be applied, depending on the reagent, to see the results. The steps for locating the bands of macromolecules, applying reagents, and detecting the effect of the reagent on the macromolecule can be automated in an appropriate instrument.
摘要翻译：通过一维或二维方法（例如凝胶电泳）分离大分子产生大分子阵列，其可以转移到载体上，从而产生与凝胶上相同的阵列。在一维凝胶电泳的情况下，由于凝胶泳道的规则间隔和大分子的可预测的迁移方向，阵列中的大分子斑点或条带的位置可以预测至少在该区域内的对应于凝胶泳道的支撑物。当感兴趣的大分子的分子量是已知的时，分子量标记可用于确定大分子带在载体上的位置，即使大分子没有在凝胶或载体上染色。可以通过将试剂点在与大分子带相交的一行中的小体积的一系列小体积的载体上，将大分子的迁移方向对应于大分子的迁移方向凝胶。可以根据试剂适用检测方法来查看结果。用于定位大分子带，应用试剂和检测试剂对大分子的影响的步骤可以在合适的仪器中自动化。

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