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1. 发明申请

US20050164404A1 Diagnostic testing process 有权
标题翻译：诊断测试过程
公开(公告)号：US20050164404A1
公开(公告)日：2005-07-28
申请号：US10497925
申请日：2002-12-12
申请人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
发明人： David Marlborugh , Andrew Sloane , Robert Cole , William Hunter
IPC分类号： G01N33/52 , B01L3/00 , G01N33/48 , G01N33/543 , G01N33/545 , G01N33/553 , G01N33/558
CPC分类号： B01L3/5023 , B01L2200/0642 , B01L2300/0825 , B01L2300/16 , B01L2400/0406 , B01L2400/0633 , G01N33/54306 , G01N33/54366 , Y02A50/58 , Y10S435/81 , Y10S435/973 , Y10S436/807 , Y10S436/81
摘要： A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed (typically for the presence of a particular protein), and a detection analyte (typically one or more antibodies bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre-incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound. In one version the pre-incubation chamber is supported above the second membrane in one position but can be pushed into contact with the membrane carrying the capture analyte thus permitting fluid transfer from the incubation chamber through the capture membrane. In another version the membrane at the base of the incubation chamber is hydrophobic and its underside contacts the capture membrane and when a wetting agent is applied to the contents of the pre-incubation chamber fluid transfer occurs.
摘要翻译：公开了一种用于流过测定过程的方法和装置。该方法的特征在于“预孵育步骤”，其中待分析的样品（通常为特定蛋白质的存在）和检测分析物（通常为结合胶体金或荧光标记的一种或多种抗体）已知结合特定蛋白质可以结合在一起所需的时间段。此预孵育步骤在样品和检测分析物的混合物与结合到膜的捕获分析物接触之前发生。提供预孵育步骤具有提高测定的灵敏度并降低测定所需样品的体积的效果。公开了一种用于实施该方法的装置，其限定用于接收样品的预孵育室和具有由膜和第二膜限定的基底的检测分析物，捕获分析物与该第二膜结合。在一个版本中，预孵育室在一个位置上被支撑在第二膜上方，但是可以被推入与承载捕获分析物的膜接触，从而允许流体从孵育室通过捕获膜转移。在另一个版本中，孵育室底部的膜是疏水性的并且其下侧接触捕获膜，并且当将润湿剂施加到预孵育室的内容物时，发生流体转移。

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