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    • 2. 发明申请
    • PRODUCTION OF BETA-LACTAM ANTIBIOTICS
    • 生产β-LACTAM抗生素
    • WO2008040731A3
    • 2008-05-22
    • PCT/EP2007060460
    • 2007-10-02
    • DSM IP ASSETS BVBOER REMONHANS MARCUSBOVENBERG ROELOF ARY LANSKLAASSEN PAULLAAN VAN DER JAN METSKE
    • HANS MARCUSBOVENBERG ROELOF ARY LANSKLAASSEN PAULLAAN VAN DER JAN METSKE
    • C12N9/02C12P37/00
    • C12N9/0004
    • The present invention describes a process for the production of an N-a-amino-hydroxyphenylacetyl or an N-a-aminophenylacetyl ß-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl- valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N- hydroxyphenylglycyl or the N-phenylglycyl ß-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V. An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing ß-lactam antibiotics with N-a-amino-hydroxyphenylacetyl or an N-a-aminophenylacetyl side chains.
    • 本发明描述了一种生产Na-氨基 - 羟基苯基乙酰基或一种氨基苯乙酰基β-内酰胺抗生素的方法,其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰缬氨酸(HpgCV)或苯基甘氨酰 - 半胱氨酰缬氨酸(HpgCV) PgCV)分别与N-羟基苯基甘氨酰或N-苯基甘氨酰ß-内酰胺抗生素反应。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸(Hpg)或苯基甘氨酸(Pg),半胱氨酸(C)和缬氨酸(V)与非核糖体肽合成酶(NRPS)接触来进一步制备,以形成 三肽HpgCV或三肽PgCV,NRPS包括对于Hpg或Pg特有的第一模块M1,对C特异的第二模块M2和对于V特定的第三模块M3。进一步提供IPNS,其在该转换中具有改善的活性,如 以及催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。
    • 6. 发明申请
    • NOVEL UPASES AND USES THEREOF
    • NOVEL加强和使用它
    • WO2007096201A2
    • 2007-08-30
    • PCT/EP2007001693
    • 2007-02-22
    • DSM IP ASSETS BVLAAN VAN DER JAN METSKESCHOONEVELD-BERGMANS MARGOT EL
    • LAAN VAN DER JAN METSKESCHOONEVELD-BERGMANS MARGOT EL
    • C07K14/37
    • C12N9/20
    • The present invention to newly identified polynucleotide sequences comprising genes that encode novel lipolytic enzymes LIP01-LIP03. The LIP01 enzyme may be isolated from Magnaporthe grisae, the LIP02 and LIP03 may be obtained by mutating the polynucleotide sequence encoding for LIP01. The invention features the full length coding sequence of the novel gene, which is suitable for expression in a suitable host cell as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods of using these proteins in industrial processes, for example in baking industry, vegetable oil degumming, production or modification of food emulsifiers and the production of glucose from wheat gluten.
    • 本发明涉及新鉴定的包含编码新型脂解酶LIP01-LIP03的基因的多核苷酸序列。 LIP01酶可从Magnaporthe grisae分离,LIP02和LIP03可通过突变编码LIP01的多核苷酸序列获得。 本发明的特征在于新基因的全长编码序列,其适于在合适的宿主细胞中表达以及全长功能性蛋白质的氨基酸序列和该基因或氨基酸序列的功能等价物。 本发明还涉及在工业过程中使用这些蛋白质的方法,例如在烘焙工业,植物油脱胶,食品乳化剂的生产或改性以及从小麦谷蛋白生产葡萄糖中。
    • 8. 发明申请
    • PROLINE-SPECIFIC ENDOPROTEASE AND USE THEREOF
    • 特异性内切酶及其用途
    • WO2015185592A3
    • 2016-02-11
    • PCT/EP2015062327
    • 2015-06-03
    • DSM IP ASSETS BV
    • LAAN VAN DER JAN METSKEVONDERVOORT VAN DE PETER JOZEF IDACHRISTIS CHANTALSPAANS MARTINEBRUINE-PAULUS DE ANGELA
    • C12N9/62
    • C12N9/62C12C11/00C12Y304/21026
    • The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65°C for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of M144L, S398F, V460A, I204F, Y205N, F279S, F263Y, P377A, L470P, and P477A, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
    • 本发明涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其中当多肽已经在65℃的温度下保持15分钟时,多肽具有小于70%的残留活性。 本发明还涉及具有脯氨酸特异性内切蛋白酶活性的多肽,其包含根据SEQ ID NO:1的氨基酸序列,其中SEQ ID NO:1包含至少一个选自M144L,S398F,V460A的氨基酸取代 ,I204F,Y205N,F279S,F263Y,P377A,L470P和P477A,编码具有脯氨酸特异性内切蛋白酶活性的多肽的核酸,制备具有脯氨酸特异性内切蛋白酶活性的变体多肽的方法,重组宿主细胞和方法 以及制备其中使用多肽的食品或饲料产品的方法。