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    • 1. 发明申请
    • NOVEL UPASES AND USES THEREOF
    • NOVEL加强和使用它
    • WO2007096201A2
    • 2007-08-30
    • PCT/EP2007001693
    • 2007-02-22
    • DSM IP ASSETS BVLAAN VAN DER JAN METSKESCHOONEVELD-BERGMANS MARGOT EL
    • LAAN VAN DER JAN METSKESCHOONEVELD-BERGMANS MARGOT EL
    • C07K14/37
    • C12N9/20
    • The present invention to newly identified polynucleotide sequences comprising genes that encode novel lipolytic enzymes LIP01-LIP03. The LIP01 enzyme may be isolated from Magnaporthe grisae, the LIP02 and LIP03 may be obtained by mutating the polynucleotide sequence encoding for LIP01. The invention features the full length coding sequence of the novel gene, which is suitable for expression in a suitable host cell as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods of using these proteins in industrial processes, for example in baking industry, vegetable oil degumming, production or modification of food emulsifiers and the production of glucose from wheat gluten.
    • 本发明涉及新鉴定的包含编码新型脂解酶LIP01-LIP03的基因的多核苷酸序列。 LIP01酶可从Magnaporthe grisae分离,LIP02和LIP03可通过突变编码LIP01的多核苷酸序列获得。 本发明的特征在于新基因的全长编码序列,其适于在合适的宿主细胞中表达以及全长功能性蛋白质的氨基酸序列和该基因或氨基酸序列的功能等价物。 本发明还涉及在工业过程中使用这些蛋白质的方法,例如在烘焙工业,植物油脱胶,食品乳化剂的生产或改性以及从小麦谷蛋白生产葡萄糖中。