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1. 发明申请

WO2015181310A2 ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION 审中-公开
标题翻译：异柠檬酸和甲基丙烯酸甲酯和二甲醚生产
公开(公告)号：WO2015181310A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061882
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries
IPC分类号： C12N15/63
CPC分类号： C12P7/44 , C07K14/395 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/62 , C12Y203/03001 , C12Y401/01006 , C12Y401/01031 , C12Y401/01032 , C12Y604/01001
摘要： The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: - a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and - a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯中的一种或多种的重组酵母细胞。本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸; 以及 - 编码催化向乙酰辅酶A反应的多肽的核酸。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯，衣康酸1-甲酯或衣康酸1,4-二甲酯的方法。

2. 发明申请

WO2014202616A2 RASAMSONIA GENE AND USE THEREOF 审中-公开
标题翻译： RASAMSONIA基因及其用途
公开(公告)号：WO2014202616A2
公开(公告)日：2014-12-24
申请号：PCT/EP2014/062737
申请日：2014-06-17
申请人： DSM IP ASSETS B.V.
发明人： WU, Liang
IPC分类号： C07K14/37
CPC分类号： C07K14/37
摘要： The invention relates to apolypeptide which comprises (a) an amino acid sequence set out in SEQ ID NO: 20501 to 32240; (b) an amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 1 to 20500; (c) or a variant polypeptide, wherein the variant polypeptide (i) has at least 70% sequence identity with the sequence set out in SEQ ID NO: 20501 to 32240, or (ii) has an amino acid sequence that differs in, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 amino acids from the amino acid sequence of SEQ ID NO: 20501 to 32240. The invention also relates to methods for using the polypeptide in industrial processes. 1 Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
摘要翻译：本发明涉及多肽，其包含（a）SEQ ID NO：20501至32240所示的氨基酸序列; （b）由SEQ ID NO：1至20500的核酸序列编码的氨基酸序列; （c）或变体多肽，其中所述变体多肽（i）与SEQ ID NO：20501至32240所示的序列具有至少70％的序列同一性，或（ii）具有不同于2的氨基酸序列，3,4,5,6,7,8,9,10,11或12个氨基酸。本发明还涉及在工业过程中使用该多肽的方法。本发明还包括用适于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

3. 发明申请

WO2013110673A1 DITERPENE PRODUCTION 审中-公开
标题翻译：地面生产
公开(公告)号：WO2013110673A1
公开(公告)日：2013-08-01
申请号：PCT/EP2013/051262
申请日：2013-01-23
申请人： DSM IP ASSETS B.V.
发明人： LEHMANN, Martin , TRUEHEART, Joshua , ZWARTJENS, Priscilla , WU, Liang , BOER, Viktor , SAGT, Cornelis, Maria, Jacobus , KUMAR, Manoj , MEIJRINK, Bernard
IPC分类号： A23L1/236 , C12N9/02 , C12N9/88 , C12N9/90 , C12P5/00
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
摘要翻译：本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

4. 发明申请

WO2009113853A3 ADIPATE ESTER OR THIOESTER SYNTHESIS 审中-公开
公开(公告)号：WO2009113853A3
公开(公告)日：2009-09-17
申请号：PCT/NL2009/050115
申请日：2009-03-11
申请人： DSM IP ASSETS B.V. , WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
发明人： WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
IPC分类号： C12P7/44
摘要： The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid(6-ACA), a method for preparing 5-formyl valeric acid (5- FVA), and a method for preparingcaprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

5. 发明申请

WO2015181312A2 CELLS FOR ITACONIC ACID PRODUCTION 审中-公开
标题翻译：用于生产酸的细胞
公开(公告)号：WO2015181312A2
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061884
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , GELDER, VAN, Marina
IPC分类号： C12N15/52 , C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12N15/52 , C12P7/46
摘要： The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid,4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which over expresses: -a nucleic 1 acid encoding a polypeptide having cis-aconitate decarboxylase activity; and-one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA,wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans- aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
摘要翻译：本发明涉及能够产生衣康酸，衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组细胞，其中所述重组微生物已经在其基因组中被修饰，从而导致其中的缺陷产生反式乌头碱甲基转移酶。能够产生衣康酸并且过表达的重组酵母细胞： - 编码具有顺 - 乌头酸脱羧酶活性的多肽的核酸1酸; 一个或多个编码多肽的核酸，其单独或一起催化向乙酰CoA的反应，其中所述重组微生物已经在其基因组中被修饰，从而导致转基因甲基转移酶的产生缺陷。这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。

6. 发明申请

WO2011009859A1 PROCESS FOR THE PREPARATION OF 1,4-BUTANEDIAMINE VIA N-ACYL OR N-GUANIDYL PROTECTED 1,4-BUTANEDIAMINE PRECURSORS 审中-公开
标题翻译：通过N-乙酰基或N-胍基保护的1,4-丁二烯前体制备1,4-丁二胺的方法
公开(公告)号：WO2011009859A1
公开(公告)日：2011-01-27
申请号：PCT/EP2010/060480
申请日：2010-07-20
申请人： DSM IP ASSETS B.V. , WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
发明人： WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
IPC分类号： C12P13/00
CPC分类号： C12P13/001
摘要： The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a - biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.
摘要翻译：本发明涉及一种制备1,4-二氨基丁烷[DAB]的新方法。根据本发明的方法涉及至少一种生物催化步骤，其包括至少一种N-保护的DAB前体的生物催化产生。本发明还涉及一种用于制备涉及至少一个生物催化步骤的DAB的方法，并且包括以下步骤：a）生物催化制备D受体的N-保护的前体，得到含有N-保护的DAB前体的生物催化反应混合物 b）从生物催化反应混合物回收N-保护的前体，和c）将N-保护的前体转化成DAB。更具体地说，本发明涉及一种制备DAB的方法，其中DAB的至少N-保护的前体选自N5保护的鸟氨酸，N-保护的DAB和N-保护的4- 氨基丁。

7. 发明申请

WO2010102982A1 METHOD FOR IMPROVING THE YIELD OF A POLYPEPTIDE 审中-公开
标题翻译：改善多肽的含量的方法
公开(公告)号：WO2010102982A1
公开(公告)日：2010-09-16
申请号：PCT/EP2010/052918
申请日：2010-03-08
申请人： DSM IP Assets B.V. , LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
发明人： LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
IPC分类号： C12P21/02 , C12N15/67
CPC分类号： C12P21/02
摘要： The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.
摘要翻译：本发明涉及提高蛋白质产量的方法。该方法包括将一组相关蛋白质特征的值修改为落入最佳范围内，或者变得更接近于真核宿主中的一种或多种蛋白质特征的最佳值。

8. 发明申请

WO2010031772A2 ALTERNATIVE BUTANOL PRODUCTION PROCESS IN A MICROBIAL CELL 审中-公开
标题翻译：一种微生物细胞中的替代丁醇生产工艺
公开(公告)号：WO2010031772A2
公开(公告)日：2010-03-25
申请号：PCT/EP2009/061961
申请日：2009-09-15
申请人： DSM IP ASSETS B.V. , WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
发明人： WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
IPC分类号： C12N15/53 , C12N15/54 , C12N9/02 , C12N9/04 , C12N9/10 , C12P7/16
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1022 , C12N9/1029 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
摘要翻译：本发明涉及包含编码酶的基因的微生物细胞，所述酶催化下列反应a）丙酮酸转化为乙酰乳酸，b）乙酰乳酸转化为2,3-二羟基异戊酸，c）2,3-二羟基异戊酸转化成2 d）2-酮异戊酸酯与异丁酰-CoA，e）异丁酰-CoA与丁酰-CoA，f）丁酰-CoA与丁醛，和g）丁醛与丁醇，其中所述细胞产生丁醇。本发明还涉及生产丁醇的方法，其中使用了根据本发明的微生物细胞。

9. 发明申请

WO2009013159A3 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
公开(公告)号：WO2009013159A3
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059119
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
IPC分类号： C12N15/81 , C12P7/16
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

10. 发明申请

WO2015181311A1 PROCESS FOR PRODUCING ITACONIC ACID AND ITACONIC ACID ESTERS UNDER ANAEROBIC CONDITIONS 审中-公开
标题翻译：在厌氧条件下生产异柠檬酸和酸性酯的方法
公开(公告)号：WO2015181311A1
公开(公告)日：2015-12-03
申请号：PCT/EP2015/061883
申请日：2015-05-28
申请人： DSM IP ASSETS B.V.
发明人： ZHAO, Zheng , MEIJRINK, Bernard , HOEVEN, VAN DER, Robertus Antonius Mijndert , WU, Liang , ROUBOS, Johannes Andries , BROERS, Nicolette Jasmijn , HARTMANS, Sybe
IPC分类号： C12P7/46 , C12P7/62
CPC分类号： C12P7/62 , C12P7/46
摘要： The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.
摘要翻译：本发明涉及一种生产衣康酸或衣康酸酯的方法，该方法包括在厌氧条件下，在合适的发酵培养基中发酵能够生产衣康酸或衣康酸酯的重组细胞，其中衣康酸产生酸或衣康酸的酯。

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