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1. 发明申请

WO2010102976A1 PREGASTRIC ESTERASE AND DERIVATIVES THEREOF 审中-公开
标题翻译： PREGASTRIC ESTERASE及其衍生物
公开(公告)号：WO2010102976A1
公开(公告)日：2010-09-16
申请号：PCT/EP2010/052903
申请日：2010-03-08
申请人： DSM IP ASSETS B.V. , PARENICOVA, Lucie , BUSINK, Ronald , LAAN, VAN DER, Jan Metske
发明人： PARENICOVA, Lucie , BUSINK, Ronald , LAAN, VAN DER, Jan Metske
IPC分类号： C12N9/20 , A23C19/032 , C12N1/15 , C12N15/21 , C12H1/00 , C12P21/02
CPC分类号： A23C19/063 , A23C19/0328 , A23C19/072 , A23C19/082 , C12N9/20
摘要： The present invention relates to novel lipase polynucleotide sequences, their corresponding proteins as well as ways of manufacturing said sequences and said proteins and use of the proteins in the preparation of food compositions. The invention further relates to methods for releasing proteins from the exterior of a host cell as well as to a method for killing micro-organisms.
摘要翻译：本发明涉及新的脂肪酶多核苷酸序列，其相应的蛋白质以及制备所述序列和所述蛋白质的方法以及蛋白质在制备食物组合物中的用途。本发明还涉及从宿主细胞外部释放蛋白质的方法以及杀死微生物的方法。

2. 发明申请

WO2007062936A2 DNA BINDING SITE OF A TRANSCRIPTIONAL ACTIVATOR USEFUL IN GENE EXPRESSION 审中-公开
标题翻译：转录激活因子基因表达的DNA结合位点
公开(公告)号：WO2007062936A2
公开(公告)日：2007-06-07
申请号：PCT/EP2006/067734
申请日：2006-10-24
申请人： DSM IP ASSETS B.V. , PARENICOVA, Lucie , PEIJ, VAN, Noël Nicolaas Maria Elisabeth
发明人： PARENICOVA, Lucie , PEIJ, VAN, Noël Nicolaas Maria Elisabeth
IPC分类号： C12N15/80
CPC分类号： C12N15/80
摘要： We have discovered DNA binding sites which are specifically recognized by PrtT, a transcriptional activator for protease genes. The DNA binding site can be defined structurally by a consensus nucleotide sequence and functionally by PrtT's ability to regulate transcriptional activation through that sequence. Both PrtT and its cognate DNA binding site (i.e., the nucleotide sequence in each promoter that is recognized by PrtT) can be used in a gene expression system. Possession of only a PrtT transcriptional activator is insufficient, its cognate DNA binding site is necessary for recognition by PrtT (i.e., binding to the site and activating transcription under appropriate conditions). A functional site, such as one obtained from a wild-type fungal gene, will confer PrtT- dependent transcriptional activation on 3'-downstream sequences. A mutation of a wild- type promoter that results in a non-functional site will abolish PrtT-dependent transcriptional activation of 3'-downstream sequences. A mutation of a wild-type promoter that results in a more functional site will enhance PrtT-dependent transcriptional activation of 3'-downstream sequences.
摘要翻译：我们发现了特异性被蛋白酶基因转录激活因子PrtT识别的DNA结合位点。 DNA结合位点可以由共有核苷酸序列在结构上定义，并在功能上由PrtT通过该序列调节转录激活的能力来定义。 PrtT及其同源DNA结合位点（即每个启动子中被PrtT识别的核苷酸序列）都可用于基因表达系统。仅拥有PrtT转录激活因子是不够的，其同源DNA结合位点对于PrtT识别是必需的（即，在适当条件下与该位点结合并激活转录）。功能位点，例如从野生型真菌基因获得的位点，将赋予3'-下游序列上PrtT依赖性转录激活。导致非功能性位点的野生型启动子的突变将消除3'-下游序列的PrtT依赖性转录激活。导致更多功能位点的野生型启动子的突变将增强3'下游序列的PrtT依赖性转录激活。

3. 发明申请

WO2010102982A1 METHOD FOR IMPROVING THE YIELD OF A POLYPEPTIDE 审中-公开
标题翻译：改善多肽的含量的方法
公开(公告)号：WO2010102982A1
公开(公告)日：2010-09-16
申请号：PCT/EP2010/052918
申请日：2010-03-08
申请人： DSM IP Assets B.V. , LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
发明人： LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
IPC分类号： C12P21/02 , C12N15/67
CPC分类号： C12P21/02
摘要： The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.
摘要翻译：本发明涉及提高蛋白质产量的方法。该方法包括将一组相关蛋白质特征的值修改为落入最佳范围内，或者变得更接近于真核宿主中的一种或多种蛋白质特征的最佳值。

4. 发明申请

WO2006040312A2 FUNGAL TRANSCRIPTIONAL ACTIVATORS USEFUL IN METHODS FOR PRODUCING A POLYPEPTIDE 审中-公开
标题翻译：用于生产多肽的方法中的真菌转录激活剂
公开(公告)号：WO2006040312A2
公开(公告)日：2006-04-20
申请号：PCT/EP2005055145
申请日：2005-10-11
申请人： DSM IP ASSETS BV , PEIJ VAN NOEL NICOLAAS MARIA E , PARENICOVA LUCIE
发明人： PEIJ VAN NOEL NICOLAAS MARIA E , PARENICOVA LUCIE
IPC分类号： C12N15/31 , C07K14/37 , C12N15/67
CPC分类号： C12N15/80
摘要： The present invention relates to functional cDNA and genomic sequences encoding PrtT proteins, which have transcriptional activity on a protease promoter, to PrtT proteins and to their use. The invention further relates to two distinct types of filamentous fungal cells. Filamentous fungal cells are transformed to over-express these PrtT proteins: this type of filamentous fungus will be highly suited as protease producer. Alternatively, the endogenous prtT genes of filamentous fungal cells are inactivated: this type of filamentous fungus is highly suited for the production of any polypeptide native or not which is highly sensitive for protease degradation. The PrtT proteins of the invention provide means for identification of functional homologues in other species.
摘要翻译：本发明涉及编码PrtT蛋白质的功能性cDNA和基因组序列，其对蛋白酶启动子具有转录活性，并且涉及它们的用途。本发明还涉及两种不同类型的丝状真菌细胞。转化丝状真菌细胞以过表达这些PrtT蛋白：这种丝状真菌将非常适合作为蛋白酶生产者。或者，丝状真菌细胞的内源性prtT基因被灭活：这种类型的丝状真菌非常适合于生产对于蛋白酶降解非常敏感的天然或非天然的多肽。本发明的PrtT蛋白提供用于鉴定其他物种中的功能同系物的方法。

5. 发明申请

WO2008053019A3 METHOD FOR REDUCING THE EXPRESSION OF A GENE IN A FILAMENTOUS FUNGAL CELL 审中-公开
标题翻译：用于减少真菌真菌细胞中基因表达的方法
公开(公告)号：WO2008053019A3
公开(公告)日：2009-01-22
申请号：PCT/EP2007061768
申请日：2007-10-31
申请人： DSM IP ASSETS BV , PEIJ VAN NOEL NICOLAAS MARIA ELISABETH , PARENICOVA LUCIE , BENCINA MOJCA , IVICAK KAROLINA
发明人： PEIJ VAN NOEL NICOLAAS MARIA EISABETH , PARENICOVA LUCIE , BENCINA MOJCA , IVICAK KAROLINA
IPC分类号： C07K14/37 , C12N15/11 , C12N15/113 , C12N15/80
CPC分类号： C12N15/80 , C07K14/37 , C12N15/111 , C12N15/1137 , C12N2310/14 , C12N2320/12
摘要： The present invention relates to a method for reducing the expression of a gene in a filamentous fungal cell. The invention further relates to a modified filamentous fungal cell suitable for the production of a compound of interest, wherein, compared to the parental cell the modified cell derives from, said modified cell exhibits improved abilities for RNAi mediated reduction of gene expression of a target gene. In addition, the invention relates to a method for the production of a compound of interest in said modified filamentous fungal cell.
摘要翻译：本发明涉及一种降低丝状真菌细胞中基因表达的方法。本发明还涉及一种适用于生产目标化合物的修饰丝状真菌细胞，其中与所述修饰细胞相比，所述修饰的细胞来源的亲本细胞相比，显示改进的RNAi介导的靶基因基因表达减少的能力。此外，本发明涉及一种在所述修饰的丝状真菌细胞中生产感兴趣的化合物的方法。

6. 发明申请

WO2008053019A2 METHOD FOR REDUCING THE EXPRESSION OF A GENE IN A FILAMENTOUS FUNGAL CELL 审中-公开
标题翻译：用于减少真菌真菌细胞中基因表达的方法
公开(公告)号：WO2008053019A2
公开(公告)日：2008-05-08
申请号：PCT/EP2007/061768
申请日：2007-10-31
申请人： DSM IP ASSETS B.V. , PEIJ, VAN, Noël, Nicolaas, Maria, Elisabeth , PARENICOVA, Lucie , BENCINA, Mojca , IVICAK, Karolina
发明人： PEIJ, VAN, Noël, Nicolaas, Maria, Elisabeth , PARENICOVA, Lucie , BENCINA, Mojca , IVICAK, Karolina
IPC分类号： C12N15/09
CPC分类号： C12N15/80 , C07K14/37 , C12N15/111 , C12N15/1137 , C12N2310/14 , C12N2320/12
摘要： The present invention relates to a method for reducing the expression of a gene in a filamentous fungal cell. The invention further relates to a modified filamentous fungal cell suitable for the production of a compound of interest, wherein, compared to the parental cell the modified cell derives from, said modified cell exhibits improved abilities for RNAi mediated reduction of gene expression of a target gene. In addition, the invention relates to a method for the production of a compound of interest in said modified filamentous fungal cell.
摘要翻译：本发明涉及一种降低丝状真菌细胞中基因表达的方法。本发明还涉及一种适用于生产目标化合物的修饰丝状真菌细胞，其中与所述修饰细胞相比，所述修饰的细胞来源的亲本细胞相比，显示改进的RNAi介导的靶基因基因表达减少的能力。此外，本发明涉及一种在所述修饰的丝状真菌细胞中生产感兴趣的化合物的方法。

7. 发明申请

WO2007062936A3 DNA BINDING SITE OF A TRANSCRIPTIONAL ACTIVATOR USEFUL IN GENE EXPRESSION 审中-公开
标题翻译：用于基因表达的转录激活因子的DNA结合位点
公开(公告)号：WO2007062936A3
公开(公告)日：2007-07-26
申请号：PCT/EP2006067734
申请日：2006-10-24
申请人： DSM IP ASSETS BV , PARENICOVA LUCIE , PEIJ VAN NOEL NICOLAAS MARIA E
发明人： PARENICOVA LUCIE , PEIJ VAN NOEL NICOLAAS MARIA E
IPC分类号： C12N15/80
CPC分类号： C12N15/80
摘要： We have discovered DNA binding sites which are specifically recognized by PrtT, a transcriptional activator for protease genes. The DNA binding site can be defined structurally by a consensus nucleotide sequence and functionally by PrtT's ability to regulate transcriptional activation through that sequence. Both PrtT and its cognate DNA binding site (i.e., the nucleotide sequence in each promoter that is recognized by PrtT) can be used in a gene expression system. Possession of only a PrtT transcriptional activator is insufficient, its cognate DNA binding site is necessary for recognition by PrtT (i.e., binding to the site and activating transcription under appropriate conditions). A functional site, such as one obtained from a wild-type fungal gene, will confer PrtT- dependent transcriptional activation on 3'-downstream sequences. A mutation of a wild- type promoter that results in a non-functional site will abolish PrtT-dependent transcriptional activation of 3'-downstream sequences. A mutation of a wild-type promoter that results in a more functional site will enhance PrtT-dependent transcriptional activation of 3'-downstream sequences.
摘要翻译：我们已经发现了由蛋白酶基因的转录激活物PrtT特异性识别的DNA结合位点。 DNA结合位点可以通过共有核苷酸序列在结构上定义，并且功能上由PrtT通过该序列调节转录激活的能力。 PrtT及其同源DNA结合位点（即由PrtT识别的每个启动子中的核苷酸序列）均可用于基因表达系统。只有PrtT转录激活物的拥有不足，其同源DNA结合位点对于PrtT识别（即在适当条件下结合位点并激活转录）是必需的。诸如从野生型真菌基因获得的功能位点将赋予3'-下游序列上的PrtT依赖性转录激活。导致非功能位点的野生型启动子的突变将消除3'-下游序列的PrtT依赖性转录激活。导致更多功能位点的野生型启动子的突变将增强3'-下游序列的PrtT依赖性转录激活。

8. 发明申请

WO2006040312A3 FUNGAL TRANSCRIPTIONAL ACTIVATORS USEFUL IN METHODS FOR PRODUCING A POLYPEPTIDE 审中-公开
公开(公告)号：WO2006040312A3
公开(公告)日：2006-04-20
申请号：PCT/EP2005/055145
申请日：2005-10-11
申请人： DSM IP ASSETS B.V. , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PARENICOVA, Lucie
发明人： PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PARENICOVA, Lucie
IPC分类号： C12N15/31 , C12N15/67 , C07K14/37
摘要： The present invention relates to functional cDNA and genomic sequences encoding PrtT proteins, which have transcriptional activity on a protease promoter, to PrtT proteins and to their use. The invention further relates to two distinct types of filamentous fungal cells. Filamentous fungal cells are transformed to over-express these PrtT proteins: this type of filamentous fungus will be highly suited as protease producer. Alternatively, the endogenous prtT genes of filamentous fungal cells are inactivated: this type of filamentous fungus is highly suited for the production of any polypeptide native or not which is highly sensitive for protease degradation. The PrtT proteins of the invention provide means for identification of functional homologues in other species.

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