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1. 发明申请

WO2009013159A3 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
公开(公告)号：WO2009013159A3
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059119
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
IPC分类号： C12N15/81 , C12P7/16
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

2. 发明申请

WO2009013157A1 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇生产在一个欧洲细胞
公开(公告)号：WO2009013157A1
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059117
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine
IPC分类号： C12P7/16 , C12N15/81 , C12N9/02
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a yeast cell capable of producing butanol, comprising a nucleotide sequence encoding a butyryl-CoA dehydrogenase and at least one nucleotide sequence encoding an electron transfer flavoprotein. The invention further relates to a process for the production of butanol and the use of butanol obtainable by the process according to the present invention as a chemical or as a fuel.
摘要翻译：本发明涉及能够生产丁醇的酵母细胞，其包含编码丁酰辅酶A脱氢酶的核苷酸序列和编码电子转移黄素蛋白的至少一个核苷酸序列。本发明还涉及一种生产丁醇的方法和通过本发明的方法获得的丁醇作为化学或燃料的用途。

3. 发明申请

WO2009113853A3 ADIPATE ESTER OR THIOESTER SYNTHESIS 审中-公开
公开(公告)号：WO2009113853A3
公开(公告)日：2009-09-17
申请号：PCT/NL2009/050115
申请日：2009-03-11
申请人： DSM IP ASSETS B.V. , WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
发明人： WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
IPC分类号： C12P7/44
摘要： The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid(6-ACA), a method for preparing 5-formyl valeric acid (5- FVA), and a method for preparingcaprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

4. 发明申请

WO2011009859A1 PROCESS FOR THE PREPARATION OF 1,4-BUTANEDIAMINE VIA N-ACYL OR N-GUANIDYL PROTECTED 1,4-BUTANEDIAMINE PRECURSORS 审中-公开
标题翻译：通过N-乙酰基或N-胍基保护的1,4-丁二烯前体制备1,4-丁二胺的方法
公开(公告)号：WO2011009859A1
公开(公告)日：2011-01-27
申请号：PCT/EP2010/060480
申请日：2010-07-20
申请人： DSM IP ASSETS B.V. , WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
发明人： WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
IPC分类号： C12P13/00
CPC分类号： C12P13/001
摘要： The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a - biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.
摘要翻译：本发明涉及一种制备1,4-二氨基丁烷[DAB]的新方法。根据本发明的方法涉及至少一种生物催化步骤，其包括至少一种N-保护的DAB前体的生物催化产生。本发明还涉及一种用于制备涉及至少一个生物催化步骤的DAB的方法，并且包括以下步骤：a）生物催化制备D受体的N-保护的前体，得到含有N-保护的DAB前体的生物催化反应混合物 b）从生物催化反应混合物回收N-保护的前体，和c）将N-保护的前体转化成DAB。更具体地说，本发明涉及一种制备DAB的方法，其中DAB的至少N-保护的前体选自N5保护的鸟氨酸，N-保护的DAB和N-保护的4- 氨基丁。

5. 发明申请

WO2010102982A1 METHOD FOR IMPROVING THE YIELD OF A POLYPEPTIDE 审中-公开
标题翻译：改善多肽的含量的方法
公开(公告)号：WO2010102982A1
公开(公告)日：2010-09-16
申请号：PCT/EP2010/052918
申请日：2010-03-08
申请人： DSM IP Assets B.V. , LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
发明人： LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
IPC分类号： C12P21/02 , C12N15/67
CPC分类号： C12P21/02
摘要： The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.
摘要翻译：本发明涉及提高蛋白质产量的方法。该方法包括将一组相关蛋白质特征的值修改为落入最佳范围内，或者变得更接近于真核宿主中的一种或多种蛋白质特征的最佳值。

6. 发明申请

WO2010031772A2 ALTERNATIVE BUTANOL PRODUCTION PROCESS IN A MICROBIAL CELL 审中-公开
标题翻译：一种微生物细胞中的替代丁醇生产工艺
公开(公告)号：WO2010031772A2
公开(公告)日：2010-03-25
申请号：PCT/EP2009/061961
申请日：2009-09-15
申请人： DSM IP ASSETS B.V. , WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
发明人： WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
IPC分类号： C12N15/53 , C12N15/54 , C12N9/02 , C12N9/04 , C12N9/10 , C12P7/16
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1022 , C12N9/1029 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
摘要翻译：本发明涉及包含编码酶的基因的微生物细胞，所述酶催化下列反应a）丙酮酸转化为乙酰乳酸，b）乙酰乳酸转化为2,3-二羟基异戊酸，c）2,3-二羟基异戊酸转化成2 d）2-酮异戊酸酯与异丁酰-CoA，e）异丁酰-CoA与丁酰-CoA，f）丁酰-CoA与丁醛，和g）丁醛与丁醇，其中所述细胞产生丁醇。本发明还涉及生产丁醇的方法，其中使用了根据本发明的微生物细胞。

7. 发明申请

WO2012089613A1 PROCESS TO INCREASE THE PRODUCTION OF A SUCCINYL-COA DERIVED COMPOUND 审中-公开
标题翻译：增加化合物衍生化合物的方法
公开(公告)号：WO2012089613A1
公开(公告)日：2012-07-05
申请号：PCT/EP2011/073765
申请日：2011-12-22
申请人： DSM IP ASSETS B.V. , HANS, Marcus , DULK, DEN, Ben , WU, Liang
发明人： HANS, Marcus , DULK, DEN, Ben , WU, Liang
IPC分类号： C12N9/00 , C12N5/10
CPC分类号： C12N9/93
摘要： This invention relates to a process to improve the production of a succinyl-CoA derived compound by a eukaryotic cell. This method may be advantageously used in the biological production of e.g. adipic acid.
摘要翻译：本发明涉及通过真核细胞改善琥珀酰辅酶A衍生化合物的生产方法。该方法可有利地用于生物生产中。己二酸。

8. 发明申请

WO2009065779A1 DICARBOXYLIC ACID PRODUCTION IN A RECOMBINANT YEAST 审中-公开
标题翻译：重组酵母中的二羧酸生产
公开(公告)号：WO2009065779A1
公开(公告)日：2009-05-28
申请号：PCT/EP2008/065587
申请日：2008-11-14
申请人： DSM IP Assets B.V. , VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
发明人： VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
IPC分类号： C12N15/60 , C12N9/88 , C12P7/46
CPC分类号： C12P7/46 , C12N9/88 , Y02P20/52
摘要： The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.
摘要翻译：本发明涉及包含编码异源酶的核苷酸序列的重组酵母，其催化苹果酸向富马酸的转化。本发明还涉及一种生产二羧酸的方法，其中使用了本发明的酵母。

9. 发明申请

WO2009101180A2 PROCESS FOR THE PRODUCTION OF A DICARBOXYLIC ACID 审中-公开
标题翻译：生产二羧酸的方法
公开(公告)号：WO2009101180A2
公开(公告)日：2009-08-20
申请号：PCT/EP2009/051717
申请日：2009-02-13
申请人： DSM IP ASSETS B.V. , VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
发明人： VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
IPC分类号： C12P7/46
CPC分类号： C12P7/46
摘要： The present invention relates to a process for the production of a dicarboxylic acid wherein a eukaryotic cell is fermented in a suitable fermentation medium. The invention further relates to a eukaryotic cell comprising a nucleotide sequence encoding an enzyme which catalyses the conversion of isocitric acid to succinic acid, and a nucleotide sequence encoding an enzyme which catalyses the conversion of glyoxylic acid to malic acid.
摘要翻译：本发明涉及生产二羧酸的方法，其中真核细胞在合适的发酵培养基中发酵。本发明还涉及包含编码催化异柠檬酸向琥珀酸转化的酶的核苷酸序列的核酸细胞，以及编码催化乙醛酸向苹果酸转化的酶的核苷酸序列。

10. 发明申请

WO2009065780A1 DICARBOXYLIC ACID PRODUCTION IN EUKARYOTES 审中-公开
标题翻译：二氧化碳酸生产
公开(公告)号：WO2009065780A1
公开(公告)日：2009-05-28
申请号：PCT/EP2008/065588
申请日：2008-11-14
申请人： DSM IP ASSETS B.V. , VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
发明人： VERWAAL, René , WU, Liang , DAMVELD, Robbertus, Antonius , SAGT, Cornelis, Maria, Jacobus
IPC分类号： C12N15/60 , C12N9/88 , C12P7/46
CPC分类号： C12P7/46 , C12N9/001 , C12N9/88 , C12N15/81 , C12N15/815 , Y02P20/52
摘要： The present invention relates to a recombinant eukaryotic microbial cell comprising a nucleotide sequence encoding a heterologous enzyme catalysing the conversion from phosphoenolpyruvate to oxaloacetate whereby ATP is generated. The invention further relates to a process for the preparation of a dicarboxylic acid such as succinic acid and fumaric acid, comprising fermenting the eukaryotic microbial cell according to the invention in a suitable fermentation medium.
摘要翻译：本发明涉及重组真核生物细胞，其包含编码异源酶的核苷酸序列，所述异源酶催化由磷酸烯醇丙酮酸向草酰乙酸转化，从而产生ATP。本发明进一步涉及一种制备二羧酸如琥珀酸和富马酸的方法，其包括将本发明的真核生物细胞在合适的发酵培养基中发酵。

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