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1. 发明专利

JPH07155183A RECOMBINANT PLASMID VECTOR, BACTERIUM OF GENUS BACILLUS AND PRODUCTION OF PORCINE GROWTH HORMONE 失效
公开(公告)号：JPH07155183A
公开(公告)日：1995-06-20
申请号：JP33930093
申请日：1993-12-02
申请人： DAICEL CHEM
发明人： YAMASHITA KUNIHIKO , IKEDA TAKAYUKI
IPC分类号： C12N15/00 , C12N1/21 , C12P21/02 , C12R1/125
摘要： PURPOSE:To efficiently obtain a large amount of a natural type porcine growth hormone at a low cost in a simple step. CONSTITUTION:This recombinant plasmid vector contains a hybrid porcine growth hormonal gene composed of a genetic region coding for as signal sequence of a protein secreted by a bacterium of the genus Bacillus such as Bacillus subtilis alpha-amylase, a structural genetic region of the porcine growth hormone located on the down stream side thereof and a promoter capable of functioning with a bacterium of the genus Bacillus such as the Bacillus subtilis on the upstream side of the hybrid porcine growth hormonal gene. A bacterium of the genus Bacillus such as a low-protease Bacillus subtilis is transformed by this vector and this method for producing a large amount of porcine growth hormone is to culture the resultant transformant.

2. 发明专利

JP2001233708A ANTIMICROBIAL AGENT AND METHOD FOR PRODUCING THE SAME 失效
公开(公告)号：JP2001233708A
公开(公告)日：2001-08-28
申请号：JP2000220307
申请日：2000-07-21
申请人： DAICEL CHEM
发明人： ONO KATSUAKI , OMIYA TAKEO , YAMASHITA KUNIHIKO
IPC分类号： A01N33/12 , A01N25/00 , A01N43/40 , A01N61/00 , C08H99/00 , C08L101/00 , C08H5/00
摘要： PROBLEM TO BE SOLVED: To both obtain an antimicrobial agent capable of maintaining antimicrobial properties and high safety at a high level and an antimicrobial composition containing the antimicrobial agent. SOLUTION: This antimicrobial agent comprises a carboxyl group-containing hydrophilic polymer such as a carboxy methyl group-containing cellulose, etc., and plural quaternary ammonium salts. The quaternary ammonium salts are (a) a 8-16C alkylbenzyl 1-6C alkylammonium salt such as a benzonium salt, etc., and (b) a 18-30C alkyltri 1-4C alkylammonium salt such as a behenyltrimethyammonium salt, etc. LD 50 of the antimicrobial agent is >=2,000 mg/g. The ratio of the plural quaternary ammonium salts of the former (a)/the latter (b) is 5/95 to 50/50 (equivalent ratio).

3. 发明专利

JP2006136432A Method for evaluating respirator sensitization nature 有权
标题翻译：评估呼吸机感光度自然的方法
公开(公告)号：JP2006136432A
公开(公告)日：2006-06-01
申请号：JP2004327125
申请日：2004-11-10
申请人： Daicel Chem Ind Ltd , Kagoshima Univ , ダイセル化学工業株式会社 , 国立大学法人鹿児島大学
发明人： YAMASHITA KUNIHIKO , YAMAGISHI MANABU , AOYAMA KIMIHARU
IPC分类号： A61B5/08
摘要： PROBLEM TO BE SOLVED: To provide a sensitization nature evaluation method easily and quantitatively evaluating a respirator sensitization nature at the time when chemical substances, natural substances or others directly reach a trachea or a lung using small animals. SOLUTION: The sensitization nature evaluation method is characterized by that a living body section in the trachea or the lung is evaluated by directly administering a sample of 100 μl or less per one time into the trachea of the small animals of a body weight of 70 g or less. In the sensitization nature evaluation method, it is preferable to directly administering the sample inside of the trachea at the stage of sensitization and/or the stage of induction. Mouses may be used as the small animals. In the method of the invention, the mouth is opened using a mouth opening holding means capable of holding the mouth opening condition of the small animal, a sample administering means is inserted from the mouth of the small animal, and the sample is directly administered inside of the trachea through a pharyngeal portion. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了在化学物质，天然物质或其它物质直接通过小动物直接到达气管或肺部时，提供致敏性评价方法，容易且定量地评价呼吸器致敏性。敏感性评价方法的特征在于，通过将每次100μl以下的样品直接施用到体重小的动物的气管中来评价气管或肺中的活体部分为70g以下。在敏化性评价方法中，优选在致敏阶段和/或诱导阶段直接给予气管内的样品。鼠标可能被用作小动物。在本发明的方法中，使用能够保持小动物的开口状态的开口保持装置打开口，从小动物的口插入样本给药装置，并将样品直接内部的气管通过咽部。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2002301494A ACTIVATED SLUDGE AND WASTEWATER DISPOSAL METHOD 失效
公开(公告)号：JP2002301494A
公开(公告)日：2002-10-15
申请号：JP2001110444
申请日：2001-04-09
申请人： DAICEL CHEM
发明人： INOUE KOICHI , KAWADA NAOKI , YAMASHITA KUNIHIKO , MIYAMA KANAKO
IPC分类号： C12N1/20 , C02F3/00 , C02F3/12 , C02F3/34 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide an activated sludge and wastewater disposal method suitable for treating water containing organic phosphorous compounds. SOLUTION: The sludge is cultured in the presence of organic phosphorous compounds, thereby providing a method for selecting microorganisms excellent in decomposability of the organic phosphorous compounds. Activated sludge having the decomposability of the organic phosphorous compounds is obtained by using the selected microorganisms. This activated sludge is useful for treatment of organic phosphorous compound-containing wastewater.

5. 发明专利

JPH08196287A PRODUCTION OF 3-HYDROXY-1-PHENYLTHIO-1-BUTENE USING A MICROORGANISM 失效
公开(公告)号：JPH08196287A
公开(公告)日：1996-08-06
申请号：JP987295
申请日：1995-01-25
申请人： DAICEL CHEM
发明人： YAMASHITA KUNIHIKO , KAWADA NAOKI , HAMAYA TAKESHI , UEDA YOICHIRO
IPC分类号： C12P7/22 , C07C323/14 , C12R1/01 , C12R1/22 , C12R1/225 , C12R1/46 , C12R1/645 , C12R1/72 , C12R1/78 , C12R1/84 , C12R1/85
摘要： PURPOSE: To efficiently obtain 3-hydroxy-1-phenylthio-1-butene which is useful as a synthetic intermediate for azetidinone by allowing a microorganism capable of reducing the following ketone or its processed product to act on 3-oxo-1- phenylthio-1-butene and collecting the product. CONSTITUTION: A microorganism capable of reducing 3-oxo-1-phenylthio-1-butene, for example, Agrobacterium, Enterobacter, Citrobacter, Issatchenkia, Stephanoascus, Geotrichum, Hansenula, Streptococcus, Leuconostoc, Syringospora, Loddemyces, Lactobacillus, Candida, Saccharomyces or a processed product therefrom is allowed to act on 3-oxo-1-phenylthio-1-butene and the product is collected. Thus, this optically active 3-hydroxyl-phenylthio-1- butene, useful as a starting substance for producing azetidinone, is simply and efficiently obtained.

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