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1. 发明申请

US20070092899A1 Multiple displacement amplification with blocker DNA 审中-公开
标题翻译：具有阻断DNA的多位置扩增
公开(公告)号：US20070092899A1
公开(公告)日：2007-04-26
申请号：US11509996
申请日：2006-08-24
申请人： Christine Hara , Allen Christian
发明人： Christine Hara , Allen Christian
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2537/163 , C12Q2537/1373
摘要： A method of whole genome amplification of a nucleic acid sample comprising providing a starting template of the nucleic acid sample, bringing the template together with blocking DNA, bringing the template together with DNA polymerase, and multiple displacement amplification of the template. The method can be used with a starting template of less than 1 ng of the nucleic acid sample.
摘要翻译：一种核酸样品的全基因组扩增方法，包括提供核酸样品的起始模板，使模板与阻断DNA一起，将模板与DNA聚合酶一起，以及模板的多重置换扩增。该方法可以与小于1ng核酸样品的起始模板一起使用。

2. 发明申请

US20060160116A1 Repetitive sequence-free DNA libraries 审中-公开
标题翻译：重复序列的DNA文库
公开(公告)号：US20060160116A1
公开(公告)日：2006-07-20
申请号：US11299347
申请日：2005-12-08
申请人： Allen Christian , Larry Dugan , Joel Bedford
发明人： Allen Christian , Larry Dugan , Joel Bedford
IPC分类号： C40B40/08 , C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6841 , C12Q1/6876 , C12Q2537/161
摘要： A method of creating a repetitive sequence-free DNA library comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, and adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library. The invention also provides a method of creating a whole chromosome painting probe comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library, and labeling the repetitive sequence-free DNA library to produce the whole chromosome painting probe. The invention also provides a method of in-situ hybridization comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library, labeling the repetitive sequence-free DNA library to produce the whole chromosome painting probe, and using the painting probe in in-situ hybridization.
摘要翻译：一种产生无重复序列的DNA文库的方法，包括提供DNA文库，从DNA文库提供扩增混合物，并向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库的步骤。本发明还提供了一种创建整个染色体绘画探针的方法，其包括以下步骤：提供DNA文库，提供来自DNA文库的扩增混合物，向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库并标记重复序列的DNA文库以产生整个染色体绘图探针。本发明还提供了一种原位杂交方法，其包括提供DNA文库，从DNA文库提供扩增混合物，向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库，标记重复无序DNA文库产生全染色体绘图探针，并在原位杂交中使用绘图探针。

3. 发明申请

US20060134772A1 System for locating cells and for cellular analysis 审中-公开
标题翻译：用于定位细胞和细胞分析的系统
公开(公告)号：US20060134772A1
公开(公告)日：2006-06-22
申请号：US11283454
申请日：2005-11-17
申请人： Robin Miles , Jackie Crawford , Allen Christian
发明人： Robin Miles , Jackie Crawford , Allen Christian
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0864 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487
摘要： A system for locating a cell for analysis. A capture structure is provided with a flow channel in the capture structure and a capture well in the flow channel. A fluid flows along the fluid flow channel and through the capture well. The cell flows with the fluid along the flow channel into the capture well but no further. In one embodiment a capture structure is provided and a flow channel and capture well are located in the capture structure. A fluid carrying the cell flows along the fluid flow channel and into the capture well. The cell flows into the capture well but no further.
摘要翻译：用于定位细胞以进行分析的系统。捕获结构具有捕获结构中的流动通道和流动通道中的捕获阱。流体沿着流体流动通道并通过捕获阱流动。细胞随着流体沿流动通道流入捕获阱，但不再进一步流动。在一个实施例中，提供捕获结构，并且流动通道和捕获阱位于捕获结构中。携带细胞的流体沿着流体流动通道流入捕获阱。细胞流入捕获阱，但不再进一步。

4. 发明申请

US20070107538A1 Biobriefcase aerosol collector heater 失效
标题翻译： Biobriefcase气溶胶集热器
公开(公告)号：US20070107538A1
公开(公告)日：2007-05-17
申请号：US11497908
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N15/0606 , C12N1/06 , G01N1/2208 , G01N1/44 , G01N2015/0088 , G01N2015/0261
摘要： A system for sampling air and collecting particles potentially including bioagents entrained in the air for detection. The system comprises collecting a sample of the air with the particles entrained in the air, directing the sample to a receiving surface, directing a liquid to the receiving surface thereby producing a liquid surface, wherein the particles potentially including bioagents become captured in the liquid, and heating the liquid wherein the particles potentially including bioagents become heated to lysis the bioagents.
摘要翻译：一种用于对空气进行采样和收集颗粒的系统，其中可能包括携带在空气中的生物信号进行检测。该系统包括用携带在空气中的颗粒收集空气样品，将样品引导到接收表面，将液体引导到接收表面，从而产生液体表面，其中潜在地包括生物标记的颗粒被捕获在液体中，并加热液体，其中潜在地包括生物体的颗粒被加热以裂解生物制剂。

5. 发明申请

US20050272042A1 Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions 有权
标题翻译：在基于DNA聚合酶的合成反应中连续添加短DNA寡核苷酸
公开(公告)号：US20050272042A1
公开(公告)日：2005-12-08
申请号：US10727779
申请日：2003-12-03
申请人： Shea Gardner , Raymond Mariella , Allen Christian , Jennifer Young , David Clague
发明人： Shea Gardner , Raymond Mariella , Allen Christian , Jennifer Young , David Clague
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.
摘要翻译：制备用户定义序列的DNA分子的方法。该方法包括以下步骤：预先选择将包含用户定义序列的DNA分子的多个DNA序列片段，以及将多个DNA序列片段与至少一种聚合酶结合， DNA序列片段连接以产生用户定义序列的DNA分子。序列段可以是长度为n，其中n是偶或奇整数。在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。在一个实施方案中，起始序列片段具有不同长度，n，n + 1，n + 2等

6. 发明申请

US20050059029A1 Derivatized versions of ligase enzymes for constructing DNA sequences 失效
标题翻译：用于构建DNA序列的连接酶的衍生版本
公开(公告)号：US20050059029A1
公开(公告)日：2005-03-17
申请号：US10718856
申请日：2003-11-21
申请人： Raymond Mariella , Allen Christian , James Tucker , John Dzenitis , Alexandros Papavasiliou
发明人： Raymond Mariella , Allen Christian , James Tucker , John Dzenitis , Alexandros Papavasiliou
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/10 , C12P19/34
摘要： A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.
摘要翻译：制备非常长的双链合成多核苷酸的方法。提供多种短寡核苷酸。短寡核苷酸相互杂交。寡核苷酸的酶连接提供了预定序列的连续的PCR-准备DNA片段。

7. 发明申请

US20070292889A1 Immunoassay magnetic trapping device 审中-公开
标题翻译：免疫测定磁捕获装置
公开(公告)号：US20070292889A1
公开(公告)日：2007-12-20
申请号：US11455211
申请日：2006-06-16
申请人： Christopher Bailey , Perry Bell , Kodumudi Venkateswaran , Allen Christian , Elizabeth Wheeler
发明人： Christopher Bailey , Perry Bell , Kodumudi Venkateswaran , Allen Christian , Elizabeth Wheeler
IPC分类号： G01N33/53 , C12M3/00 , G01N33/551
CPC分类号： B01L3/502761 , B01L3/527 , B01L7/52 , B01L2200/0668 , B01L2200/141 , B01L2300/0867 , B01L2300/0877 , B01L2400/043
摘要： A system for immunoassaying a sample comprising providing magnetic beads, connecting signal molecules to the beads, connecting the sample to the magnetic beads with the connected signal molecules, magnetically trapping the magnetic beads with the connected signal molecules and the sample, lysising the sample, and analyzing the sample.
摘要翻译：一种用于免疫测定样品的系统，包括提供磁珠，将信号分子连接到珠，将样品连接到具有连接的信号分子的磁珠，用连接的信号分子和样品磁性捕获磁珠，裂解样品，以及分析样品。

8. 发明申请

US20070122025A1 AUTOMATED MACROMOLECULAR CRYSTAL DETECTION SYSTEM AND METHOD 失效
标题翻译：自动化大分子晶体检测系统及方法
公开(公告)号：US20070122025A1
公开(公告)日：2007-05-31
申请号：US10452668
申请日：2003-05-30
申请人： Allen Christian , Brent Segelke , Bernard Rupp , Dominique Toppani
发明人： Allen Christian , Brent Segelke , Bernard Rupp , Dominique Toppani
IPC分类号： G06K9/00
CPC分类号： G06K9/4609 , G06K9/522
摘要： An automated macromolecular method and system for detecting crystals in two-dimensional images, such as light microscopy images obtained from an array of crystallization screens. Edges are detected from the images by identifying local maxima of a phase congruency-based function associated with each image. The detected edges are segmented into discrete line segments, which are subsequently geometrically evaluated with respect to each other to identify any crystal-like qualities such as, for example, parallel lines, facing each other, similarity in length, and relative proximity. And from the evaluation a determination is made as to whether crystals are present in each image.
摘要翻译：用于检测二维图像中的晶体的自动化大分子方法和系统，例如从结晶屏阵列获得的光学显微镜图像。通过识别与每个图像相关联的基于相位一致性的函数的局部最大值，从图像中检测边缘。检测到的边缘被分割成离散线段，随后几何地相对于彼此估计，以识别任何类似晶体的质量，例如平行线，彼此面对，长度相似性和相对接近度。从评估结果可以确定每个图像中是否存在晶体。

9. 发明申请

US20070107539A1 Biobriefcase aerosol collector 失效
标题翻译： Biobriefcase气溶胶收集器
公开(公告)号：US20070107539A1
公开(公告)日：2007-05-17
申请号：US11497918
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N15/0606 , G01N1/2208 , G01N1/44 , G01N2015/0088 , G01N2015/0261
摘要： A system for sampling air and collecting particles entrained in the air that potentially include bioagents. The system comprises providing a receiving surface, directing a liquid to the receiving surface and producing a liquid surface. Collecting samples of the air and directing the samples of air so that the samples of air with particles entrained in the air impact the liquid surface. The particles potentially including bioagents become captured in the liquid. The air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid but cause minor turbulence. The liquid surface has a surface tension and the collector samples the air and directs the air to the liquid surface so that the air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid, but cause minor turbulence on the surface resulting in insignificant evaporation of the liquid.
摘要翻译：一种用于对空气进行取样并收集在空气中的颗粒的系统，其中可能包括生物标签。该系统包括提供接收表面，将液体引导到接收表面并产生液体表面。收集空气的样品并引导空气样品，使得夹带在空气中的颗粒的空气样品冲击液体表面。潜在地包括生物标记的颗粒被捕获在液体中。夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流。液体表面具有表面张力，并且收集器对空气进行采样并将空气引导到液体表面，使得夹带在空气中的颗粒的空气以足够的速度冲击液体表面以将颗粒夹带入液体中，但是引起轻微的紊流在表面上导致液体的微不足道的蒸发。

10. 发明申请

US20070107537A1 Biobriefcase electrostatic aerosol collector 失效
标题翻译： Biobriefcase静电吸尘器
公开(公告)号：US20070107537A1
公开(公告)日：2007-05-17
申请号：US11497897
申请日：2006-08-01
申请人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
发明人： Perry Bell , Allen Christian , Christopher Bailey , Ladona Willis , Donald Masquelier , Shanavaz Nasarabadi
IPC分类号： G01N1/22
CPC分类号： G01N1/2208 , G01N2001/2217
摘要： A system for sampling air and collecting particles entrained in the air comprising a receiving surface, a liquid input that directs liquid to the receiving surface and produces a liquid surface, an air input that directs the air so that the air with particles entrained in the air impact the liquid surface, and an electrostatic contact connected to the liquid that imparts an electric charge to the liquid. The particles potentially including bioagents become captured in the liquid by the air with particles entrained in the air impacting the liquid surface. Collection efficiency is improved by the electrostatic contact electrically charging the liquid. The effects of impaction and adhesion due to electrically charging the liquid allows a unique combination in a particle capture medium that has a low fluid consumption rate while maintaining high efficiency.
摘要翻译：用于对空气进行采样并收集夹带在空气中的颗粒的系统，包括接收表面，将液体引导到接收表面并产生液体表面的液体输入;引导空气的空气输入，使得具有夹带在空气中的颗粒的空气冲击液体表面，以及连接到液体上的电荷的静电接触。潜在地包括生物标记的颗粒被空气捕获在液体中，颗粒夹带在空气中，撞击液体表面。通过使液体充电的静电接触来提高收集效率。由于对液体进行充电而引起的冲击和粘附的影响允许在保持高效率的同时具有低流体消耗率的颗粒捕获介质中的独特组合。

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