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1. 发明申请

US20060160116A1 Repetitive sequence-free DNA libraries 审中-公开
标题翻译：重复序列的DNA文库
公开(公告)号：US20060160116A1
公开(公告)日：2006-07-20
申请号：US11299347
申请日：2005-12-08
申请人： Allen Christian , Larry Dugan , Joel Bedford
发明人： Allen Christian , Larry Dugan , Joel Bedford
IPC分类号： C40B40/08 , C12Q1/68
CPC分类号： C12N15/1093 , C12Q1/6841 , C12Q1/6876 , C12Q2537/161
摘要： A method of creating a repetitive sequence-free DNA library comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, and adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library. The invention also provides a method of creating a whole chromosome painting probe comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library, and labeling the repetitive sequence-free DNA library to produce the whole chromosome painting probe. The invention also provides a method of in-situ hybridization comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library, labeling the repetitive sequence-free DNA library to produce the whole chromosome painting probe, and using the painting probe in in-situ hybridization.
摘要翻译：一种产生无重复序列的DNA文库的方法，包括提供DNA文库，从DNA文库提供扩增混合物，并向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库的步骤。本发明还提供了一种创建整个染色体绘画探针的方法，其包括以下步骤：提供DNA文库，提供来自DNA文库的扩增混合物，向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库并标记重复序列的DNA文库以产生整个染色体绘图探针。本发明还提供了一种原位杂交方法，其包括提供DNA文库，从DNA文库提供扩增混合物，向扩增混合物中加入重复序列级分DNA以产生无重复序列的DNA文库，标记重复无序DNA文库产生全染色体绘图探针，并在原位杂交中使用绘图探针。

2. 外观设计

USD700270S1 Spinner blade 有权
标题翻译：旋转刀片
公开(公告)号：USD700270S1
公开(公告)日：2014-02-25
申请号：US29457213
申请日：2013-06-07
申请人： Joseph Bonner , Daniel Bonner , Joseph R. Bonner , Larry Dugan
设计人： Joseph Bonner , Daniel Bonner , Joseph R. Bonner , Larry Dugan

3. 发明授权

US06675679B2 Internal gripping pipe wrench 失效
标题翻译：内部夹紧扳手
公开(公告)号：US06675679B2
公开(公告)日：2004-01-13
申请号：US10189968
申请日：2002-07-03
申请人： Larry Dugan
发明人： Larry Dugan
IPC分类号： B25B2308
CPC分类号： B25B13/5083
摘要： Disclosed is a wrench that grips the internal surface of a pipe to be turned. When torque is applied to the wrench, one or more gripping surfaces are forced apart by a cam mechanism. The gripping surfaces tightly engage the internal surface of the pipe in response to movement of an internal cam. Torque applied to the wrench causes the pipe to turn. Because the wrench grips the internal diameter of a pipe, it can be used without damaging the external surface of the pipe, and used in very confined spaces. The gripping surfaces distribute the gripping load evenly across the pipe, which minimizes any deformation of the pipe.
摘要翻译：公开了一种夹持待转动管道的内表面的扳手。当扭矩施加到扳手时，一个或多个夹紧表面被凸轮机构强制分开。抓握表面响应于内部凸轮的移动紧密地接合管子的内表面。扭矩施加在扳手上会使管道转动。因为扳手夹住管道的内径，所以可以在不损坏管道的外表面的情况下使用，并用于非常狭窄的空间。抓握表面将抓握载荷均匀地分布在管道上，这最小化管道的任何变形。

4. 发明授权

US06904835B2 Mechanical coupler 失效
标题翻译：机械耦合器
公开(公告)号：US06904835B2
公开(公告)日：2005-06-14
申请号：US10670175
申请日：2003-09-23
申请人： Larry Dugan , Steven X. Johnson
发明人： Larry Dugan , Steven X. Johnson
IPC分类号： B25B13/50 , B25B23/08
CPC分类号： B25B13/5083
摘要： Disclosed is a coupler that grips the internal surface of a torque receptor such as a pipe or driveshaft to be turned. When torque is applied to the coupler, one or more gripping surfaces are forced apart by a cam mechanism. The gripping surfaces tightly engage the internal surface of the pipe in response to movement of an internal cam. Torque applied to the wrench causes the pipe to turn. Because the wrench grips the internal diameter of a torque receptor, it can be used without damaging the external surface of the torque receptor, and used in very confined spaces. The gripping surfaces distribute the gripping load evenly across the pipe, which minimizes any deformation of the pipe.
摘要翻译：公开了一种联接器，其夹持扭矩接收器的内表面，例如要转动的管道或驱动轴。当扭矩施加到联接器时，一个或多个夹紧表面被凸轮机构强制分开。抓握表面响应于内部凸轮的移动紧密地接合管子的内表面。扭矩施加在扳手上会使管道转动。由于扳手夹紧扭矩接收器的内径，因此可以在不损坏扭力传感器的外表面的情况下使用，并用于非常狭窄的空间。抓握表面将抓握载荷均匀地分布在管道上，这最小化管道的任何变形。

5. 发明授权

US06851342B2 Anti-ovaling mechanical coupler 失效
标题翻译：抗椭圆机械耦合器
公开(公告)号：US06851342B2
公开(公告)日：2005-02-08
申请号：US10670174
申请日：2003-09-23
申请人： Larry Dugan , Steven X. Johnson , Henry J. Gohlke , Jianmin Li
发明人： Larry Dugan , Steven X. Johnson , Henry J. Gohlke , Jianmin Li
IPC分类号： B25B13/50 , B25B23/08
CPC分类号： B25B13/5083
摘要： Disclosed is a coupler that grips the internal surface of a torque receptor such as a pipe or driveshaft to be turned. When torque is applied to the coupler, one or more gripping surfaces are forced apart by a cam mechanism. The gripping surfaces tightly engage the internal surface of the pipe in response to movement of an internal cam. Torque applied to the wrench causes the pipe to turn. Because the wrench grips the internal diameter of a torque receptor, it can be used without damaging the external surface of the torque receptor, and used in very confined spaces. The gripping surfaces distribute the gripping load evenly across the pipe, which minimizes any deformation of the pipe. The pipe is engaged by the gripping surfaces beyond the threaded portion of the pipe, where the pipe has more structure, to prevent ovaling.
摘要翻译：公开了一种联接器，其夹持扭矩接收器的内表面，例如要转动的管道或驱动轴。当扭矩施加到联接器时，一个或多个夹紧表面被凸轮机构强制分开。抓握表面响应于内部凸轮的移动紧密地接合管子的内表面。扭矩施加在扳手上会使管道转动。由于扳手夹紧扭矩接收器的内径，因此可以在不损坏扭力传感器的外表面的情况下使用，并用于非常狭窄的空间。抓握表面将抓握载荷均匀地分布在管道上，这最小化管道的任何变形。管道被夹紧表面超过管道的螺纹部分接合，其中管道具有更多的结构，以防止椭圆形。

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