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1. 发明授权

US09359634B2 Fast reaction kinetics of enzymes having low activity in dry chemistry layers 有权
标题翻译：在干化学层中具有低活性的酶的快速反应动力学
公开(公告)号：US09359634B2
公开(公告)日：2016-06-07
申请号：US13210564
申请日：2011-08-16
申请人： Carina Horn , Claudia Gaessler-Dietsche , Dieter Heindl , Joachim Hoenes , Thomas Meier , Rainer Schmuck
发明人： Carina Horn , Claudia Gaessler-Dietsche , Dieter Heindl , Joachim Hoenes , Thomas Meier , Rainer Schmuck
IPC分类号： C12Q1/54 , C12N11/00 , C12N11/02 , C12N11/14 , C12N9/04 , C12Q1/32
CPC分类号： C12Q1/54 , C12N9/0006 , C12N11/00 , C12N11/02 , C12N11/14 , C12Q1/32
摘要： The present invention concerns a method for determining an analyte as well as a diagnostic element suitable therefore. In one particular form, a method for determining an analyte includes contacting a sample containing the analyte with a diagnostic element comprising a dry reagent layer. The dry reagent layer contains a mutated dehydrogenase which is specific for the analyte and an artificial coenzyme. The method also includes determining at least one of analyte presence and an amount of the analyte.
摘要翻译：本发明涉及一种用于确定分析物的方法以及因此合适的诊断元件。在一种具体形式中，用于确定分析物的方法包括使含有分析物的样品与包含干试剂层的诊断元件接触。干试剂层含有对分析物和人工辅酶特异的突变脱氢酶。该方法还包括确定分析物存在和分析物的量中的至少一种。

2. 发明申请

US20140322737A1 FAST REACTION KINETICS OF ENZYMES HAVING LOW ACTIVITY IN DRY CHEMISTRY LAYERS 有权
标题翻译：在干燥化学物质层中具有低活性的酶的快速反应动力学
公开(公告)号：US20140322737A1
公开(公告)日：2014-10-30
申请号：US13210564
申请日：2011-08-16
申请人： Carina Horn , Claudia Gaessler-Dietsche , Dieter Heindl , Joachim Hoenes , Thomas Meier , Rainer Schmuck
发明人： Carina Horn , Claudia Gaessler-Dietsche , Dieter Heindl , Joachim Hoenes , Thomas Meier , Rainer Schmuck
IPC分类号： C12Q1/54 , C12Q1/32
CPC分类号： C12Q1/54 , C12N9/0006 , C12N11/00 , C12N11/02 , C12N11/14 , C12Q1/32
摘要： The present invention concerns a method for determining an analyte as well as a diagnostic element suitable therefore. In one particular form, a method for determining an analyte includes contacting a sample containing the analyte with a diagnostic element comprising a dry reagent layer. The dry reagent layer contains a mutated dehydrogenase which is specific for the analyte and an artificial coenzyme. The method also includes determining at least one of analyte presence and an amount of the analyte.
摘要翻译：本发明涉及一种用于确定分析物的方法以及因此合适的诊断元件。在一种具体形式中，用于确定分析物的方法包括使含有分析物的样品与包含干试剂层的诊断元件接触。干试剂层含有对分析物和人工辅酶特异的突变脱氢酶。该方法还包括确定分析物存在和分析物的量中的至少一种。

3. 发明申请

US20120130062A1 ENZYMATIC SYNTHESIS OF CARBA-NAD 有权
标题翻译： CARBA-NAD的酶促合成
公开(公告)号：US20120130062A1
公开(公告)日：2012-05-24
申请号：US13358146
申请日：2012-01-25
申请人： Hartmut Duefel , Dieter Heindl , Carina Horn , Thomas Meier , Rainer Schmuck
发明人： Hartmut Duefel , Dieter Heindl , Carina Horn , Thomas Meier , Rainer Schmuck
IPC分类号： C07H19/207 , C12P19/32
CPC分类号： C07H19/207 , C12P19/32
摘要： The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.
摘要翻译：本公开内容涉及烟酰胺腺嘌呤二核苷酸NAD / NADH和烟酰胺腺嘌呤二核苷酸磷酸NADP / NADPH的稳定类似物的酶合成，即所谓的“carba-NAD”，即NAD / NADH或NADP / NADPH的类似物，分别包含碳环糖代替核糖。

4. 发明授权

US08580949B2 Enzymatic synthesis of carba-NAD 有权
标题翻译：碳酰化酶的酶合成
公开(公告)号：US08580949B2
公开(公告)日：2013-11-12
申请号：US13358146
申请日：2012-01-25
申请人： Hartmut Duefel , Dieter Heindl , Carina Horn , Thomas Meier , Rainer Schmuck
发明人： Hartmut Duefel , Dieter Heindl , Carina Horn , Thomas Meier , Rainer Schmuck
IPC分类号： C07H19/207
CPC分类号： C07H19/207 , C12P19/32
摘要： The disclosure concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called “carba-NADs”, i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.
摘要翻译：本公开内容涉及烟酰胺腺嘌呤二核苷酸NAD / NADH和烟酰胺腺嘌呤二核苷酸磷酸NADP / NADPH的稳定类似物的酶合成，即所谓的“carba-NAD”，即NAD / NADH或NADP / NADPH的类似物，分别包含碳环糖代替核糖。

5. 发明申请

US20120276565A1 STABILIZATION OF ENZYMES WITH STABLE COENZYMES 有权
标题翻译：用稳定的聚苯乙烯稳定酶
公开(公告)号：US20120276565A1
公开(公告)日：2012-11-01
申请号：US13400209
申请日：2012-02-20
申请人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
发明人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
IPC分类号： C12N9/96 , G01N27/26 , G01N21/76 , C12Q1/32 , C12Q1/26
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
摘要翻译：公开了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。此外，还公开了用稳定的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。

6. 发明授权

US09540702B2 Stabilization of enzymes with stable coenzymes 有权
标题翻译：用稳定的辅酶稳定酶
公开(公告)号：US09540702B2
公开(公告)日：2017-01-10
申请号：US13400209
申请日：2012-02-20
申请人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
发明人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
IPC分类号： C12N9/04
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
摘要翻译：公开了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。此外，还公开了用稳定的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。

7. 发明授权

US07972830B2 Thermostable Taq polymerase fragment 有权
标题翻译：热稳定性Taq聚合酶片段
公开(公告)号：US07972830B2
公开(公告)日：2011-07-05
申请号：US10917157
申请日：2004-08-12
申请人： Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
发明人： Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
摘要翻译：发现缺乏288个N-末端氨基酸（Taq＆Dgr; 288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段具有比TaqWT，Taq＆Dgr; 279和Taq＆Dgr; 289增加的热稳定性。因此，本发明提供Taq＆Dgr; 288，编码其相同或衍生物的重组表达载体，以及Taq＆Dgr; 288的纯化方案。本发明还包括含有Taq＆Dgr; 288的试剂盒，以及使用Taq＆Dgr; 288和含有Taq＆Dgr; 288的试剂盒。此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。

8. 发明申请

US20050037412A1 Thermostable Taq polymerase fragment 有权
标题翻译：热稳定性Taq聚合酶片段
公开(公告)号：US20050037412A1
公开(公告)日：2005-02-17
申请号：US10917157
申请日：2004-08-12
申请人： Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
发明人： Thomas Meier , Waltraud Ankenbauer , Annette Deufel , Dieter Heindl , Gisela Betzl , Rainer Schmuck , Bernd Schneidinger , Jessica Strey
IPC分类号： C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
CPC分类号： C12N9/1252
摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
摘要翻译：发现缺乏288个N末端氨基酸（TaqDelta288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段具有比TaqWT，TaqDelta279和TaqDelta289更高的热稳定性。因此，本发明提供TaqDelta288，编码其相同或衍生的重组表达载体，以及TaqDelta288的纯化方案。本发明还包括含有TaqDelta288的试剂盒以及使用TaqDelta288和含有TaqDelta288的试剂盒。此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。

9. 发明授权

US08129106B2 Sequence-specific detection of methylation in biomolecules 有权
标题翻译：生物分子中甲基化的序列特异性检测
公开(公告)号：US08129106B2
公开(公告)日：2012-03-06
申请号：US11570672
申请日：2005-06-14
申请人： Elmar Weinhold , Thomas Meier , Hartmut Düfel , Christine Markert-Hahn , Rainer Schmuck
发明人： Elmar Weinhold , Thomas Meier , Hartmut Düfel , Christine Markert-Hahn , Rainer Schmuck
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/53 , C07H15/00 , C07H21/04 , C07H19/04
CPC分类号： C07H19/16 , C12Q1/6827 , C12Q2537/164 , C12Q2522/101 , C12Q2521/125
摘要： A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.
摘要翻译：一种用于检测生物分子中序列特异性甲基化的方法，包括：（a）在所述甲基转移酶的可检测辅因子的存在下，使所述生物分子与S-腺苷-L-甲硫氨酸依赖性甲基转移酶接触; 和（b）检测所述甲基转移酶的识别序列是否已经用辅因子或其衍生物修饰，其中所述甲基转移酶的识别序列的修饰指示在所述识别序列处不存在甲基化。还公开了对S-腺苷-L-甲硫氨酸依赖性甲基转移酶特异性的辅因子，其中所述辅因子是N-腺苷氮丙啶衍生物，其报告基团连接到腺嘌呤环的6或7位或连接到氮丙啶环上。还公开了辅因子和甲基转移酶的复合物，其包含辅因子或复合物以及辅因子或复合物用于检测DNA分子中的序列特异性甲基化的组合物。

10. 发明申请

US20110165564A1 SEQUENCE-SPECIFIC DETECTION OF METHYLATION IN BIOMOLECULES 有权
标题翻译：生物分子中甲基化的序列特异性检测
公开(公告)号：US20110165564A1
公开(公告)日：2011-07-07
申请号：US11570672
申请日：2005-06-14
申请人： Elmar Weinhold , Thomas Meier , Hartmut Düfel , Christine Markert-Hahn , Rainer Schmuck
发明人： Elmar Weinhold , Thomas Meier , Hartmut Düfel , Christine Markert-Hahn , Rainer Schmuck
IPC分类号： C12Q1/68 , C07H19/22 , C07H21/04
CPC分类号： C07H19/16 , C12Q1/6827 , C12Q2537/164 , C12Q2522/101 , C12Q2521/125
摘要： A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.
摘要翻译：一种用于检测生物分子中序列特异性甲基化的方法，包括：（a）在所述甲基转移酶的可检测辅因子的存在下，使所述生物分子与S-腺苷-L-甲硫氨酸依赖性甲基转移酶接触; 和（b）检测所述甲基转移酶的识别序列是否已经用辅因子或其衍生物修饰，其中所述甲基转移酶的识别序列的修饰指示在所述识别序列处不存在甲基化。还公开了对S-腺苷-L-甲硫氨酸依赖性甲基转移酶特异性的辅因子，其中所述辅因子是N-腺苷氮丙啶衍生物，其报告基团连接到腺嘌呤环的6或7位或连接到氮丙啶环上。还公开了辅因子和甲基转移酶的复合物，其包含辅因子或复合物以及辅因子或复合物用于检测DNA分子中的序列特异性甲基化的组合物。

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