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1. 发明申请

WO2009067174A1 NORMALIZATION METHODS FOR G-PROTEIN COUPLED RECEPTOR MEMBRANE ARRAY 审中-公开
标题翻译： G蛋白偶联受体膜阵列的正常化方法
公开(公告)号：WO2009067174A1
公开(公告)日：2009-05-28
申请号：PCT/US2008/012805
申请日：2008-11-14
申请人： CORNING INCORPORATED , DONG, Longying , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , LIU, Li , LYNN, Jeffrey G
发明人： DONG, Longying , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , LIU, Li , LYNN, Jeffrey G
IPC分类号： G01N33/50
CPC分类号： G01N33/554 , G01N2333/726 , G01N2500/10
摘要： Reference membrane components are either pre-labeled or labeled during assays for purposes of normalizing signals associated with binding or functional assays employing G-protein coupled receptor microarrays. A reference component may be included in a membrane in which the target GPCR is embedded or may be present in another membrane printed in conjunction with the target membrane on a microspot. Or, a GPCR microarray may be pre-labeled by incorporating a label on an exposed substrate in a defect in the printed microspot.
摘要翻译：参考膜组分在测定期间被预标记或标记，目的是使与G-蛋白偶联受体微阵列的结合或功能测定相关的信号归一化。参考组分可以包含在其中嵌入目标GPCR的膜中，或者可以存在于与微孔上的靶膜结合印刷的另一膜中。或者，GPCR微阵列可以通过在印刷的微点的缺陷中的暴露的基底上并入标签来进行预标记。

2. 发明申请

WO2005010532A1 MULTIPLEXED BINDING ASSAYS FOR RECEPTOR ARRAYS 审中-公开
标题翻译：用于受体阵列的多重绑定测定
公开(公告)号：WO2005010532A1
公开(公告)日：2005-02-03
申请号：PCT/US2004/019750
申请日：2004-06-21
申请人： CORNING INCORPORATED , FANG, Ye , FERRIE, Ann M , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , PENG, Jinlin , WEBB, Brian L
发明人： FANG, Ye , FERRIE, Ann M , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , PENG, Jinlin , WEBB, Brian L
IPC分类号： G01N33/566
CPC分类号： C40B30/04 , G01N33/74 , G01N2333/726
摘要： A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.
摘要翻译：提供了一种专题微阵列和在存在或不存在目标化合物的情况下使用标记配体的混合溶液进行多重结合测定的方法。这些方法使研究人员可以使用微阵列格式来筛选多个靶点的化合物。

3. 发明申请

WO2013116449A1 AUTOMATIC CONTINUOUS PERFUSION CELL CULTURE MICROPLATE CONSUMABLES 审中-公开
标题翻译：自动连续灌注细胞培养微生物消耗
公开(公告)号：WO2013116449A1
公开(公告)日：2013-08-08
申请号：PCT/US2013/024030
申请日：2013-01-31
申请人： CORNING INCORPORATED , GORAL, Vasiliy Nikolaevich , LAI, Fang , YUEN, Po Ki
发明人： GORAL, Vasiliy Nikolaevich , LAI, Fang , YUEN, Po Ki
IPC分类号： C12M1/32 , C12M1/00 , C12M1/42
CPC分类号： C12M29/10 , B32B37/12 , C12M23/12 , C12M29/04 , C12M35/08 , Y10T156/10 , Y10T156/1064
摘要： A microplate for culturing cells with automatic, continuous perfusion includes a well frame and a planar substrate. The well frame and the planar substrate form a first well, a second well, and a third well. The first well is fluidly connected with the second well with a first perfusion membrane and the first well is fluidly connected with the third well with a second perfusion membrane. Methods of fabricating the microplate and methods of culturing cells are also provided.
摘要翻译：用于用自动连续灌注培养细胞的微板包括井架和平面基底。井架和平面底板形成第一井，第二井和第三井。第一个井用第一个灌注膜与第二个井流体连接，第一个井用第二个灌注膜与第三个井流体连接。还提供了制备微孔板的方法和培养细胞的方法。

4. 发明申请

WO2012128962A1 LABEL-FREE RIGID CELL ASSAY METHOD 审中-公开
标题翻译：无标签电池测试方法
公开(公告)号：WO2012128962A1
公开(公告)日：2012-09-27
申请号：PCT/US2012/028241
申请日：2012-03-08
申请人： CORNING INCORPORATED , LAI, Fang , LI, Guangshan , LIU, Anping , PENG, Jinlin
发明人： LAI, Fang , LI, Guangshan , LIU, Anping , PENG, Jinlin
IPC分类号： G01N21/55
CPC分类号： G01N21/553
摘要： A label-free cell assay method including: culturing rigid cells in a buffer, the rigid cells having a diameter of about 3 to about 7 micrometers; depositing the rigid cells on the surface of a sensor system; and detecting the rigid cells with the sensor when exposed to two or more wavelengths of light having two or more penetration depths, as defined herein. Also disclosed are assay methods for contacting the rigid cells with a substance and determining the response of the contacted rigid cells to the substance, such as a drug candidate or modulator compound, as defined herein.
摘要翻译：无标记细胞测定方法，包括：在缓冲液中培养刚性细胞，刚性细胞具有约3至约7微米的直径; 将刚性电池沉积在传感器系统的表面上; 并且当暴露于具有两个或更多个穿透深度的两个或更多波长的光，如本文所定义时，用传感器检测刚性单元。还公开了用于使刚性细胞与物质接触并测定接触的刚性细胞对如本文所定义的药物候选物或调节剂化合物的物质的反应的测定方法。

5. 发明申请

WO2007078926A3 FLUORESCENT DYES 审中-公开
公开(公告)号：WO2007078926A3
公开(公告)日：2007-07-12
申请号：PCT/US2006/048307
申请日：2006-12-19
申请人： CORNING INCORPORATED , GASPER, Susan M , HE, Mingqian , LAI, Fang , LESLIE, Thomas M , SAMSON, Helen
发明人： GASPER, Susan M , HE, Mingqian , LAI, Fang , LESLIE, Thomas M , SAMSON, Helen
IPC分类号： C09B23/10 , C09B23/14 , G01N33/52
摘要： Fluorescent dyes useful for detecting a target material in biological assays an electron donating moiety which is linked by a conjugated π-electron bridge to an electron accepting moiety. The π-electron bridge includes at least one carbocyclic ring structure or heterocyclic ring structure. The electron accepting moiety is a carboxylic acid, a salt of a carboxylic acid, or has the formula (I) wherein X 8 is selected from the group consisting of H, CH 3 , and (CH 2 ) n6 -Z 2 - , where Z 2 - is a monovalent anion; and n 6 is an integer from 1 through 10; and Q 3 is O or S.

6. 发明申请

WO2004012658A2 METHOD AND DEVICE FOR PROTEIN DELIVERY INTO CELLS 审中-公开
标题翻译：蛋白质递送到细胞中的方法和装置
公开(公告)号：WO2004012658A2
公开(公告)日：2004-02-12
申请号：PCT/US2003/023879
申请日：2003-07-30
申请人： CORNING INCORPORATED
发明人： FANG, Ye , LAI, Fang , PICARD, Laurent, Alain, Gabriel , WEBB, Brian, L.
IPC分类号： A61K
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
摘要翻译：提供了将表面介导的蛋白质递送到活细胞中并制备蛋白质转染的细胞簇阵列的方法。该方法包括提供含蛋白质的混合物; 将所述含蛋白质的混合物在限定的位置沉积在表面上; 将含蛋白质的混合物作为微滴固定在表面上; 并以足够的密度和将蛋白质递送到细胞中的条件将细胞电镀在表面上。含蛋白质的混合物包含任何合适的氨基酸序列，包括肽，蛋白质，蛋白质结构域，抗体或蛋白质 - 核酸缀合物等与载体试剂。蛋白质转染的细胞阵列可用于在活细胞的天然环境中快速和直接地筛选蛋白质或酶功能或任何给定的细胞内蛋白质相互作用，以及用于其他生物和化学分析物的高通量筛选，其中影响这些蛋白质的功能。

7. 发明公开

EP1525473A2 METHOD AND DEVICE FOR PROTEIN DELIVERY INTO CELLS 有权
标题翻译：行政蛋白在细胞的方法
公开(公告)号：EP1525473A2
公开(公告)日：2005-04-27
申请号：EP03766993.4
申请日：2003-07-30
申请人： CORNING INCORPORATED
发明人： FANG, Ye , LAI, Fang , PICARD, Laurent, Alain, Gabriel , WEBB, Brian, L.
IPC分类号： G01N33/50 , G01N33/68 , C12N15/00
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

8. 发明授权

EP1525473B1 METHOD FOR PROTEIN DELIVERY INTO CELLS 有权
标题翻译：行政蛋白在细胞的方法
公开(公告)号：EP1525473B1
公开(公告)日：2009-03-18
申请号：EP03766993.4
申请日：2003-07-30
申请人： Corning Incorporated
发明人： FANG, Ye , LAI, Fang , PICARD, Laurent, Alain, Gabriel , WEBB, Brian, L.
IPC分类号： G01N33/50 , G01N33/68 , C12N15/00
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

9. 发明公开

EP1646871A1 MULTIPLEXED BINDING ASSAYS FOR RECEPTOR ARRAYS 审中-公开
标题翻译： MULTIPLEX结合分析FOR受体阵列
公开(公告)号：EP1646871A1
公开(公告)日：2006-04-19
申请号：EP04776836.1
申请日：2004-06-21
申请人： Corning Incorporated
发明人： FANG, Ye , FERRIE, Ann M , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , PENG, Jinlin , WEBB, Brian L
IPC分类号： G01N33/566 , G01N33/543 , G01N33/68
CPC分类号： C40B30/04 , G01N33/74 , G01N2333/726
摘要： A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.

10. 发明公开

EP2220490A1 NORMALIZATION METHODS FOR G-PROTEIN COUPLED RECEPTOR MEMBRANE ARRAY 审中-公开
标题翻译：归一化法G蛋白偶联受体膜组
公开(公告)号：EP2220490A1
公开(公告)日：2010-08-25
申请号：EP08851118.3
申请日：2008-11-14
申请人： Corning Incorporated
发明人： DONG, Longying , HONG, Yulong , LAHIRI, Joydeep , LAI, Fang , LIU, Li , LYNN, Jeffrey G
IPC分类号： G01N33/50
CPC分类号： G01N33/554 , G01N2333/726 , G01N2500/10
摘要： Reference membrane components are either pre-labeled or labeled during assays for purposes of normalizing signals associated with binding or functional assays employing G-protein coupled receptor microarrays. A reference component may be included in a membrane in which the target GPCR is embedded or may be present in another membrane printed in conjunction with the target membrane on a microspot. Or, a GPCR microarray may be pre-labeled by incorporating a label on an exposed substrate in a defect in the printed microspot.

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