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1. 发明专利

JP2004161685A Yolk-related protein and application thereof 审中-公开
公开(公告)号：JP2004161685A
公开(公告)日：2004-06-10
申请号：JP2002330226
申请日：2002-11-14
申请人： Bl:Kk , Frontier Science Co Ltd , 株式会社ビーエル , 株式会社フロンティア・サイエンス
发明人： HARA AKIHIKO , NANBA YASUHARU , ITO KEIZO
IPC分类号： G01N33/53 , C07K14/46 , C07K16/18 , G01N33/543 , G01N33/545 , G01N33/553
摘要： PROBLEM TO BE SOLVED: To provide a substitution method of a conventional measuring system of an environmental hormone disruptor by detecting vitellogenin by providing a new female-specific yolk-related protein (YRP) derived from Oryzias latipes and an antibody against the protein. SOLUTION: The female-specific yolk-related protein derived from the Oryzias latipes has substantially 460 kDa molecular weight measured by gel filtration, and substantially 130 kDa molecular weight measured by dodecyl sodium sulfate-polyacrylamide gel electrophoresis. The antibody against the protein, and various kinds of immunological measuring methods of the yolk-related protein by using the antibody are also provided. COPYRIGHT: (C)2004,JPO

2. 发明专利

JP2002214236A METHOD AND DEVICE FOR DETECTING ANTIGEN IN BLOOD 失效
公开(公告)号：JP2002214236A
公开(公告)日：2002-07-31
申请号：JP2001014310
申请日：2001-01-23
申请人： JAPAN SCIENCE & TECH CORP , BL KK , FRONTIER SCIENCE CO LTD
发明人： HARA AKIHIKO , NANBA YASUHARU , ITO KEIZO , SASAKI TATSU
IPC分类号： G01N33/543
摘要： PROBLEM TO BE SOLVED: To detect a target antigen in blood by immuno-chromatography without previously removing hemocytes only by irrigating and developing the whole blood. SOLUTION: In this device for detecting the antigen in blood comprised of a membrane carrier (3) for chromatography development, a color label first antibody to a substance to be tested, a second antibody to the substance to be tested, and a hemocyte capturing membrane member (7), the first antibody is impregnated in a label antibody impregnating member (2) arranged at the starting section of the chromatography development, the second antibody is fixed to the carrier at a capturing section (31) separated from the starting section of chromatography development, and the hemocyte capturing membrane member is comprised of a carboxymethylcellulose membrane and laminated on the upper surface of the impregnating member. Blood is made to permeate into the hemocyte capturing membrane member as it is the whole blood to make blood components except hemocytes permeate into the impregnating member and is chromatographically developed on the carrier, and a complex of the substance to be tested and the first antibody is captured at the capturing section and colored.

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