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    • 2. 发明专利
    • Reagent composition for analyzing residual leucocyte in blood bank product having reduced number of leucocyte
    • 用于分析具有减少白细胞数的血液银行产品中的残留白细胞的试剂组合物
    • JP2005043365A
    • 2005-02-17
    • JP2004213564
    • 2004-07-21
    • Bayer Corpバイエル コーポレイション
    • KUNICKA JOLANTAZELMANOVIC DAVIDFISCHER GENA
    • G01N33/49G01N1/30G01N15/14G01N33/48G01N33/50
    • G01N33/5002G01N2015/1486G01N2015/1488Y10T436/101666Y10T436/107497
    • PROBLEM TO BE SOLVED: To provide a reagent for highly accurately and precisely analyzing and counting residual leucocytes. SOLUTION: The method for counting and analyzing the residual leucocytes in a sample of a blood product selected from a group of plasma, erythrocytes, and platelets and having a reduced number of leucocytes includes a process (a) for forming a suspension of the blood product having a reduced number of leucocytes by bringing the blood product having a reduced number of leucocytes into contact with a sufficient quantity of a dissolving reagent, which contains both a low molar concentration of a buffer agent (i) and a nonionic surface-active agent (ii); a process (b) for incubating the suspension of the product having a reduced number of leucocytes and the dissolving reagent at temperatures sufficient for selectively dissolving platelets and erythrocytes without damaging the leucocytes over a sufficient period of time; a process (c) for bringing the leucocytes of the dissolved blood product into contact with pixels appropriate for dyeing the leucocytes; and a process (d) for measuring the number of the dyed leucocytes. COPYRIGHT: (C)2005,JPO&NCIPI
    • 要解决的问题:提供用于高精度和精确地分析和计数残留白细胞的试剂。 解决方案:用于计数和分析选自血浆,红细胞和血小板组中并且具有减少的白细胞数的血液制品样品中的残留白细胞的方法包括:(a)用于形成 通过使具有减少数量的白细胞数量的血液制品与足量的溶解试剂接触,所述血液制品含有少量的白细胞,所述溶解试剂含有低摩尔浓度的缓冲剂(i)和非离子表面活性剂 活性剂(ii); 一种用于在足以在足以在足以在足够的时间内损伤白细胞的情况下温育足以选择性地溶解血小板和红细胞的温度的培养具有减少白细胞数的产物和溶解试剂的悬浮液的方法(b) 用于使溶解的血液制品的白细胞与适合于染色白细胞的像素接触的方法(c); 以及用于测量染色的白细胞数目的方法(d)。 版权所有(C)2005,JPO&NCIPI
    • 8. 发明专利
    • DE69835854D1
    • 2006-10-26
    • DE69835854
    • 1998-06-23
    • BAYER CORP
    • ZELMANOVIC DAVIDMOSKALSKI WILLIAMDECLERCQ RONNYLOUWET FRANKKUCKERT EBERHARD DRPODSZUN WOLFGANG DR
    • G01N15/10G01N33/49G01N15/00G01N15/14
    • The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.