专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06171463B2 Electrophoresis method and apparatus for separating bio-organic molecules 有权
标题翻译：用于分离生物有机分子的电泳方法和设备
公开(公告)号：US06171463B2
公开(公告)日：2001-01-09
申请号：US09301080
申请日：1999-04-28
申请人： Barton G. Selby , Johan Goudberg , Binh Vo , David Clark , Thomas Sch{grave over (e)}fer , Munechika Sakabe
发明人： Barton G. Selby , Johan Goudberg , Binh Vo , David Clark , Thomas Sch{grave over (e)}fer , Munechika Sakabe
IPC分类号： G01N2726
CPC分类号： G01N27/44743
摘要： The present invention relates to the electrophoretic separation of bio-organic molecules using a slab gel electrophoresis apparatus having a pair of spaced, confronting plates defining a multi-lane separation zone adapted to hold a separation medium and an upper loading zone. In one aspect of the invention, at least one of the plates is shaped to provide an expanded loading zone. The plate-to-plate distance within the expanded loading zone is greater than the plate-to-plate width within the separation zone. In another aspect of the invention, a comb is provided with each tooth having a gradual taper beginning at a support member and extending along most of the tooth's longitudinal axis, and a sharp taper beginning immediately beyond the gradual taper and extending to the end of the tooth. In a related aspect of the invention, a method is provided wherein a comb is inserted into the loading zone so that the teeth penetrate an upper portion of the separation medium, thereby forming a series of fluid-tight, elongate sample-loading apertures of substantially rectangular cross-section having a plate-to-plate width greater than that of the inter-plate distance in the separation zone. Methods of separating biomolecule analytes for optimized resolution and increased base reads are also disclosed.
摘要翻译：本发明涉及使用板式凝胶电泳装置的生物有机分子的电泳分离，所述板式凝胶电泳装置具有限定适于保持分离介质和上装载区的多通道分离区的一对间隔开的面对板。在本发明的一个方面中，至少一个板被成形为提供一个膨胀的加载区。扩展加载区内的板间距离大于分离区内的板对板宽度。在本发明的另一方面，梳子具有每个齿具有从支撑构件开始并且沿着该齿的纵向轴线的大部分延伸的渐变锥形的逐渐渐缩锥形，以及紧接着逐渐变细并且延伸至齿。在本发明的相关方面，提供了一种方法，其中将梳子插入装载区域中，使得齿穿透分离介质的上部，由此形成一系列流体密封的细长的样品加载孔，其基本上矩形横截面的板对板宽度大于分离区中的板间距离。还公开了用于优化分辨率和增加的碱基读数分离生物分子分析物的方法。

2. 发明授权

US5102623A Infinitesimal liquid reactor 失效
标题翻译：无限液体反应器
公开(公告)号：US5102623A
公开(公告)日：1992-04-07
申请号：US255474
申请日：1988-10-11
申请人： Tomoo Yamamoto , Shigeki Yagi , Munechika Sakabe , Osamu Segawa , Kiyoshi Kobayashi
发明人： Tomoo Yamamoto , Shigeki Yagi , Munechika Sakabe , Osamu Segawa , Kiyoshi Kobayashi
IPC分类号： B01L7/00 , G01N35/00 , G01N35/10
CPC分类号： B01L7/52 , G01N35/1011 , G01N2035/00435 , G01N2035/1025 , G01N2035/103 , G01N35/1083 , Y10T436/113332 , Y10T436/114165 , Y10T436/2575
摘要： An automatic infinitesimal liquid reactor equipped with a pipetter for dispensing reagents employs two stages which are capable of sliding horizontally. A microplate which has a plurality of recesses in rows is placed in one stage and reagent bottles are placed on the other stage. The pipetter is supported on a horizontal guide rail operating slidably horizontally and along an axis perpendicular to the direction in which the two stages slide. The horizontal guide rail is supported on a vertical guide rail so as to operate slidably in a vertical direction. Thus the pipetter can be moved in a plane perpendicular to the direction in which the two stages slide. When reagents are sucked from reagent bottles, the second stage is slid so that a reagent bottle is superposed below the pipetter and the pipetter is moved downwardly into the reagent bottle to draw in a reagent. When the reagent is discharged, the first stage is slid so that the microplate is superposed below the pipetter and the pipetter is moved downwardly into a recess to discharge the reagent, thereby dispensing a reagent efficiently and minimizing the space required for the reactor.

3. 发明授权

US5056427A Sealing of cavity on reagent tray 失效
标题翻译：在试剂盒上密封
公开(公告)号：US5056427A
公开(公告)日：1991-10-15
申请号：US493520
申请日：1990-03-14
申请人： Munechika Sakabe , Osamu Segawa
发明人： Munechika Sakabe , Osamu Segawa
IPC分类号： B01J19/16 , B01L3/00 , B01L7/00
CPC分类号： B01L3/50851 , B01J19/16 , B01L3/50853 , B01L7/00
摘要： A thermal reaction apparatus for sealing liquid within cavities includes a tray having cavities for storing therein liquid. The cavities have openings arranged at varying levels in thickness direction of the tray. A planar sealing member is disposed on the tray to cover the openings and is comprised of a pair of flexible sheets and viscous fluid filled between the flexible sheets. A pressure plate is disposed on the planar sealing member for applying thereto pressure effective to locally flow the viscous fluid to enable the sealing member to seal the varying levels of the cavity openings to thereby prevent evaporation of the liquid stored in the cavities. Alternatively, the elastic sealing member is comprised of an elastic sheet and projections arranged in registration with the respective cavities, and the pressure plate is disposed in contact with the projections for applying there to pressure effective to deform locally the sealing member through the projections to enable the sealing member to fittably seal the cavity openings. Or, the pressure plate may have projections, instead of the sealing member, in contact with the planar sealing member in registration to the respective cavities.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式