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1. 发明授权

US5851792A Aglucone isoflavone enriched vegetable protein whey whey protein material aglucone isoflavone material high genistein content material and high daidzein content material and process for producing the same from a vegetable protein whey 失效
标题翻译：富含葡萄糖异黄酮的植物蛋白乳清乳清蛋白材料配基异黄酮材料高染料木黄酮含量材料和高黄豆苷原含量材料及其制备方法从植物蛋白乳清
公开(公告)号：US5851792A
公开(公告)日：1998-12-22
申请号：US627301
申请日：1996-04-03
申请人： Jerome Shen , Mark A. Roussey , Barbara A. Bryan , Maryann C. Allred
发明人： Jerome Shen , Mark A. Roussey , Barbara A. Bryan , Maryann C. Allred
IPC分类号： A23J3/14 , A23J3/16 , A23L11/00 , A23L11/30 , C12P17/06 , C12P21/06 , C12P19/62
CPC分类号： A23J3/16 , C12P17/06
摘要： An aglucone isoflavone enriched vegetable protein whey, whey protein material, high genistein material, high daidzein material, and aglucone isoflavone material are provided, as well as a process for producing the same from a vegetable protein whey. Isoflavone conjugates in a vegetable protein whey are converted to isoflavone glucosides by treating the whey at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glucosides are converted to aglucone isoflavones by enzymatic reaction to produce an aglucone isoflavone enriched vegetable protein whey. Aglucone isoflavone whey protein material is recovered from the aglucone isoflavone enriched vegetable protein whey. A high genistein content material, a high daidzein content material, and an aglucone isoflavone material are produced from an alcohol extract of the aglucone isoflavone whey protein material.
摘要翻译：提供了富含配基异黄酮的植物蛋白乳清，乳清蛋白质材料，高染料木黄酮材料，高黄豆苷原物质和配基异黄酮材料，以及由植物蛋白乳清生产的方法。将植物蛋白乳清中的异黄酮共轭物通过在温度和pH下处理乳清一段时间以转化为异黄酮糖苷而转化为异黄酮糖苷。通过酶促反应将异黄酮糖苷转化成配基异黄酮，生成富含配基异黄酮的植物蛋白乳清。从配基异黄酮植物蛋白乳清中回收葡萄糖异黄酮乳清蛋白物质。高分子量染料木素含量材料，高黄豆苷元含量物质和配基异黄酮材料由配基异黄酮乳清蛋白材料的醇提取物生产。

2. 发明授权

US5827682A Two-step conversion of vegetable protein isoflavone conjugates to aglucones 失效
标题翻译：将植物蛋白异黄酮共轭物两步转化成甙元
公开(公告)号：US5827682A
公开(公告)日：1998-10-27
申请号：US730171
申请日：1996-10-15
申请人： Barbara A. Bryan , Maryann C. Allred , Mark A. Roussey
发明人： Barbara A. Bryan , Maryann C. Allred , Mark A. Roussey
IPC分类号： C07D311/36 , C07H17/07 , C12P17/06 , C21P21/06 , C07D311/64 , C12N9/24
CPC分类号： C12P17/06 , C07H17/07
摘要： Disclosed are methods for converting isoflavone conjugates in vegetable protein materials to isoflavone aglucones by forming an aqueous mixture of a vegetable protein material containing isoflavone conjugates, converting the isoflavone conjugates to isoflavone glucones, and converting the isoflavone glucones to isoflavone aglucones. The vegetable protein material may also contain isoflavone glucones which are converted to isoflavone aglucones. The isoflavone conjugates, isoflavone glucones, or isoflavone aglucones present in the vegetable protein material may be removed from the vegetable materials before, during, or after the conversion process.
摘要翻译：公开了通过形成含有异黄酮共轭物的植物蛋白材料的水性混合物，将异黄酮共轭物转化为异黄酮葡糖酸酯，并将异黄酮葡萄糖苷转化为异黄酮糖苷的方法，将植物蛋白材料中的异黄酮共轭物转化为异黄酮糖苷。植物蛋白材料还可以含有异黄酮葡萄糖甙，其被转化成异黄酮糖苷。存在于植物蛋白材料中的异黄酮共轭物，异黄酮葡萄糖甙或异黄酮甙元可以在转化过程之前，期间或之后从植物材料中除去。

3. 发明授权

US06521282B1 Aglucone isoflavone enriched vegetable flour and grit 失效
标题翻译：富含葡萄糖的异黄酮植物粉和砂砾
公开(公告)号：US06521282B1
公开(公告)日：2003-02-18
申请号：US09702265
申请日：2000-10-31
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： A23L120
CPC分类号： A23L11/33
摘要： A vegetable protein flour or grit is provided which is rich in aglucone isoflavones. In one embodiment, the vegetable protein flour or grit contains at least 400 parts per million of genistein and 400 ppm of daidzein. In another embodiment the vegetable protein flour or grit contains at least 50% genistein and daidzein relative to all genistein family isoflavones and daidzein family isoflavones, respectively.
摘要翻译：提供富含配基异黄酮的植物蛋白粉或粗粒。在一个实施方案中，植物蛋白粉或砂砾含有至少400ppm的染料木黄酮和400ppm的黄豆苷原。在另一个实施方案中，相对于所有染料木素族异黄酮和大豆黄素家族异黄酮，植物蛋白粉或砂砾分别含有至少50％的染料木黄酮和黄豆苷原。

4. 发明授权

US5726034A Aglucone isoflavone enriched vegetable protein extract and protein material, and high genistein and daidzein content materials and process for producing the same 失效
标题翻译：富含肌苷异黄酮的植物蛋白提取物和蛋白质材料，高染料木黄酮和黄豆苷原含量的材料及其制备方法
公开(公告)号：US5726034A
公开(公告)日：1998-03-10
申请号：US709026
申请日：1996-09-06
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： C12P17/16 , A23J3/14 , A23J3/16 , A23L11/00 , A23L11/30 , A61K31/35 , A61K31/352 , C07D311/36 , C07K14/415 , C12P17/06 , C21P21/06 , A61K35/78 , C12N9/24 , A61K31/337
CPC分类号： C12P17/06 , A23J3/14 , A23J3/16 , A23L11/07 , A23L11/33 , A23L11/34
摘要： An aglucone is isoflavone enriched vegetable protein extract and protein material are provided, as well as a high genistein content material and a high daidzein content material. Isoflavone conjugates in a vegetable material are converted to isoflavone glucosides by treating the vegetable material at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glycosides are converted to glucose isoflavones by enzymatic reaction. The vegetable material is extracted with an aqueous extractant having a pH above about the isoelectric point of protein in the vegetable material to extract protein and the isoflavones either before or after conversion of the isoflavone conjugates to isoflavone glucosides or the conversion of the isoflavone glucosides to aglucone isoflavones. An aglucone isoflavone enriched protein material is produced by precipitating the protein and aglucone isoflavones from the extract. A high genistein content material or a high daidzein content material may be produced from the aglucone isoflavone enriched protein extract or aglucone isoflavone enriched protein material by separating the high genistein or high daidzein content material from the extract or protein material.
摘要翻译：配基是富含异黄酮的植物蛋白提取物和蛋白质材料，以及高染料木黄酮含量的材料和高黄豆苷原含量的材料。将植物材料中的异黄酮共轭物通过在温度和pH下处理植物材料一段足以实现转化的时间转化为异黄酮糖苷。异黄酮糖苷通过酶反应转化成葡萄糖异黄酮。植物材料用pH高于植物原料蛋白质等电点的水提取剂提取，以在将异黄酮共轭物转化为异黄酮糖苷之前或之后提取蛋白质和异黄酮，或将异黄酮糖苷转化成配基异黄酮通过从提取物中沉淀蛋白质和配基异黄酮产生富含配基异黄酮的蛋白质材料。通过从提取物或蛋白质材料中分离高染料木黄酮或高黄豆苷原含量的材料，可以从富含配基异黄酮的蛋白质提取物或富含配基异黄酮的蛋白质材料中产生高染料木黄酮含量的材料或高黄豆苷原含量的材料。

5. 发明授权

US06579561B2 Aglucone isoflavone enriched vegetable flour and vegetable grit and process for making the same from a vegetable material containing isoflavone 失效
公开(公告)号：US06579561B2
公开(公告)日：2003-06-17
申请号：US09884217
申请日：2001-06-19
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： A23L120
CPC分类号： A23L11/33
摘要： The present invention provides a process for producing an aglucone isoflavone enriched vegetable flour or grit from a vegetable material containing isoflavone glucosides. A vegetable material containing isoflavone glucosides is slurried in water and the isoflavone glucosides are contacted with an enzyme effective to convert isoflavone glucosides to aglucone isoflavones. A vegetable flour or grit containing the aglucone isoflavones is recovered from the slurry.

6. 发明授权

US6013771A Isoflavone rich protein isolate and process for producing 失效
标题翻译：富含异黄酮的蛋白质分离和生产过程
公开(公告)号：US6013771A
公开(公告)日：2000-01-11
申请号：US93750
申请日：1998-06-09
申请人： Jerome L. Shen , Balagtas F. Guevara , Frank E. Spadafora , Barbara A. Bryan
发明人： Jerome L. Shen , Balagtas F. Guevara , Frank E. Spadafora , Barbara A. Bryan
IPC分类号： A23C11/10 , A23J1/00 , A23L1/30 , A23J1/14 , A23L2/38
CPC分类号： A23J1/006 , A23C11/106 , A23L33/105
摘要： A process for providing an isoflavone rich protein isolate is provided, along with the isoflavone rich protein isolate produced thereby. A vegetable material containing protein and at least one isoflavone compound is extracted with an aqueous extractant having a pH above the isoelectric point of the protein, and preferably an alkaline pH. The protein and isoflavones are extracted into the extractant, and the extractant containing the protein and isoflavones is separated from insoluble vegetable protein materials to form a protein extract. The pH of the protein extract is adjusted to about the isoelectric point of the protein to precipitate the protein. The extract containing the precipitated protein is cooled to a temperature of about 40.degree. F. to about 80.degree. F., and then the protein is separated from the extract. Washing of the separated protein is avoided, or, is conducted with minimum amounts of water. The cool separation temperatures and the low wash conditions unexpectedly significantly increase the concentration of isoflavones recovered in the separated protein.
摘要翻译：提供了富含异黄酮的蛋白质分离物的方法，以及由此产生的富含异黄酮的蛋白质分离物。含有蛋白质和至少一种异黄酮化合物的植物材料用pH高于蛋白质等电点的含水萃取剂提取，优选碱性pH。将蛋白质和异黄酮提取到提取剂中，将含有蛋白质和异黄酮的提取物与不溶性植物蛋白质材料分离，形成蛋白质提取物。将蛋白质提取物的pH调节至蛋白质的等电点以沉淀蛋白质。将含有沉淀蛋白质的提取物冷却至约40°F至约80°F的温度，然后将蛋白质与提取物分离。避免分离的蛋白质的洗涤，或者用最少量的水进行洗涤。冷分离温度和低洗涤条件意外地显着增加了分离的蛋白质中回收的异黄酮的浓度。

7. 发明授权

US5990291A Recovery of isoflavones from soy molasses 有权
标题翻译：从大豆糖蜜中回收异黄酮
公开(公告)号：US5990291A
公开(公告)日：1999-11-23
申请号：US204431
申请日：1998-12-03
申请人： Doyle H. Waggle , Barbara A. Bryan
发明人： Doyle H. Waggle , Barbara A. Bryan
IPC分类号： C12P17/06 , A23J1/14 , A23J3/16 , C07D311/36 , C07D311/40 , C07H17/07
CPC分类号： C07D311/40 , C07D311/36 , C07H17/07
摘要： Methods for recovering isoflavones and derivatives thereof from soy molasses are disclosed. In a first embodiment, a method is disclosed in which isoflavones are recovered without any significant conversion of isoflavone conjugates to other forms. In a second embodiment, a method is disclosed whereby isoflavone conjugates are converted to glucosides while in the soy material prior to their recovery. In a third embodiment, a method is disclosed in which isoflavones are converted to their aglucone form while in the soy material and prior to their recovery. Also disclosed are various isoflavone enriched products obtained from soy molasses.
摘要翻译：公开了从大豆糖蜜中回收异黄酮及其衍生物的方法。在第一个实施方案中，公开了一种方法，其中回收异黄酮而不将异黄酮共轭物转化为其它形式。在第二个实施方案中，公开了一种方法，其中异黄酮共轭物在其恢复之前在大豆原料中转化为葡糖苷。在第三个实施方案中，公开了一种方法，其中异黄酮在大豆原料中并在其恢复之前被转化为其配体形式。还公开了从大豆糖蜜获得的各种异黄酮富集产物。

8. 发明授权

US5637562A Aglucone isoflavone enriched vegetable protein concentrate and process for producing 失效
标题翻译：葡聚糖异黄酮富集植物蛋白浓缩物及其制备方法
公开(公告)号：US5637562A
公开(公告)日：1997-06-10
申请号：US307751
申请日：1996-04-12
申请人： Jerome L. Shen , Barbara A. Bryan
发明人： Jerome L. Shen , Barbara A. Bryan
IPC分类号： A23J3/14 , A23J3/34 , C07D311/36 , C07K1/02 , C07K1/14 , C07K1/30 , C07K1/36 , C07K14/415 , C12P17/06 , C12P17/16 , A61K31/335 , A61K38/02
CPC分类号： C07K14/415 , C12P17/06
摘要： Aglucone isoflavone enriched protein concentrates and processes for producing and recovering are disclosed. The process comprises an isoelectrie wash of a vegetable protein material to provide a protein concentrate, which is slurried and reacted with a sufficient amount of beta-glucosidase enzyme or esterase enzyme for a time period, temperature, and pH sufficient to convert at least a majority of the glucone isoflavones contained in the concentrate to aglucone isoflavones.
摘要翻译： PCT No.PCT / US94 / 10696 Sec。 371日期：1996年4月12日 102（e）日期1996年4月12日PCT 1994年9月21日PCT PCT。第WO95 / 10529号公报日期：1995年4月20日公开了富含异黄酮的蛋白质浓缩物及其制造和回收方法。该方法包括植物蛋白质材料的等离子洗涤以提供蛋白质浓缩物，其将浆化并与足够量的β-葡糖苷酶或酯酶在一定时间，温度和pH下反应，足以转化至少大部分的浓缩物中含有的葡萄糖异黄酮配成异黄酮。

9. 发明授权

US06495141B2 Recovery of isoflavones from soy molasses 有权
标题翻译：从大豆糖蜜中回收异黄酮
公开(公告)号：US06495141B2
公开(公告)日：2002-12-17
申请号：US10016461
申请日：2001-10-19
申请人： Doyle H. Waggle , Barbara A. Bryan
发明人： Doyle H. Waggle , Barbara A. Bryan
IPC分类号： A01N6500
CPC分类号： C07D311/40 , C07D311/36 , C07H17/07
摘要： Isoflavone compositions derived from soy molasses are provided. In a first embodiment, an isoflavone material derived from soy molasses containing at least two isoflavones is provided, where at least one of the isoflavones is genistein. In a second embodiment, an isoflavone material derived from soy molasses containing at least two isoflavones is provided, where at least one of the isoflavones is daidzein.
摘要翻译：提供衍生自大豆糖蜜的异黄酮组合物。在第一实施方案中，提供衍生自含有至少两种异黄酮的大豆糖蜜的异黄酮物质，其中至少一种异黄酮是染料木黄酮。在第二实施方案中，提供衍生自含有至少两种异黄酮的大豆糖蜜的异黄酮物质，其中至少一种异黄酮是黄豆苷元。

10. 发明授权

US5637561A Aglucone isoflavone enriched vegetable protein whey, whey protein, and process for producing 失效
标题翻译：富含葡聚糖的异黄酮植物蛋白乳清，乳清蛋白及其制备方法
公开(公告)号：US5637561A
公开(公告)日：1997-06-10
申请号：US307731
申请日：1996-04-12
申请人： Jerome L. Shen , Barbara A. Bryan
发明人： Jerome L. Shen , Barbara A. Bryan
IPC分类号： A23J3/14 , A23J3/34 , A23L33/00 , C07D311/36 , C07K1/14 , C07K1/30 , C07K1/34 , C07K14/415 , C12P17/06 , C12P21/02 , A61K31/335 , A61K38/02
CPC分类号： C12P17/06 , C07D311/36
摘要： Aglucone isoflavone enriched vegetable protein whey, whey protein and processes for producing and recovering such are disclosed. Aglucone isoflavone enriched vegetable protein whey is made by treating whey comprising glucone isoflavones with a sufficient amount of beta-glucosidase enzyme or esterase enzyme or acid to convert at least a majority of the glucone isoflavones to aglucones and thereby provide an aglucone enriched whey. An aglucone enriched whey protein is obtained by recovery of the protein.
摘要翻译： PCT No.PCT / US94 / 10699 Sec。 371日期：1996年4月12日 102（e）日期1996年4月12日PCT 1994年9月21日PCT PCT。第WO95 / 10512号公报日期1995年04月20日公开了富含葡萄糖的异黄酮植物蛋白乳清，乳清蛋白及其生产和回收方法。通过用足够量的β-葡糖苷酶或酯酶或酸处理包含葡糖异黄酮的乳清来将至少大部分的葡萄糖异黄酮转化为甙元，从而提供富含配基的乳清，从而制备富含异亮氨酸的富含异黄酮的植物蛋白乳清。通过回收蛋白质获得富集配基的乳清蛋白。

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