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1. 发明授权

US6083553A Recovery of isoflavones from soy molasses 失效
标题翻译：从大豆糖蜜中回收异黄酮
公开(公告)号：US6083553A
公开(公告)日：2000-07-04
申请号：US92805
申请日：1998-06-05
申请人： Doyle H. Waggle , Barbara A. Bryan
发明人： Doyle H. Waggle , Barbara A. Bryan
IPC分类号： C07D311/40 , C07D311/36
CPC分类号： C07D311/40
摘要： Methods for recovering isoflavones and derivatives thereof from soy molasses are disclosed. In a first embodiment, a method is disclosed in which isoflavones are recovered from soy molasses. In a second embodiment, a method is disclosed whereby isoflavone conjugates present in soy molasses are converted to isoflavone glucosides, and an isoflavone glucoside material is recovered from the soy molasses. In a third embodiment, a method is disclosed in which isoflavones are converted to their aglucone form, and an aglucone isoflavone material is recovered from soy molasses.
摘要翻译：公开了从大豆糖蜜中回收异黄酮及其衍生物的方法。在第一实施方案中，公开了从大豆糖蜜中回收异黄酮的方法。在第二实施方案中，公开了一种存在于大豆糖蜜中的异黄酮共轭物转化为异黄酮糖苷的方法，从大豆糖蜜中回收异黄酮糖苷物质。在第三个实施方案中，公开了一种方法，其中将异黄酮转化为其配体形式，并且从大豆糖蜜中回收配基异黄酮材料。

2. 发明授权

US5919921A Recovery of isoflavones from soy molasses 失效
公开(公告)号：US5919921A
公开(公告)日：1999-07-06
申请号：US086658
申请日：1998-05-29
申请人： Doyle H. Waggle , Barbara A. Bryan
发明人： Doyle H. Waggle , Barbara A. Bryan
IPC分类号： C12P17/06 , A23J1/14 , A23J3/16 , C07D311/36 , C07D311/40 , C07H17/07 , C07H1/08 , A61K35/78 , C07H17/04
CPC分类号： C07D311/40 , C07D311/36 , C07H17/07
摘要： Methods for recovering isoflavones and derivatives thereof from soy molasses are disclosed. In a first embodiment, a method is disclosed in which isoflavones are recovered without any significant conversion of isoflavone conjugates to other forms. In a second embodiment, a method is disclosed whereby isoflavone conjugates are converted to glucosides while in the soy material prior to their recovery. In a third embodiment, a method is disclosed in which isoflavones are converted to their aglucone form while in the soy material and prior to their recovery. Also disclosed are various isoflavone enriched products obtained from soy molasses.

3. 发明授权

US5851792A Aglucone isoflavone enriched vegetable protein whey whey protein material aglucone isoflavone material high genistein content material and high daidzein content material and process for producing the same from a vegetable protein whey 失效
标题翻译：富含葡萄糖异黄酮的植物蛋白乳清乳清蛋白材料配基异黄酮材料高染料木黄酮含量材料和高黄豆苷原含量材料及其制备方法从植物蛋白乳清
公开(公告)号：US5851792A
公开(公告)日：1998-12-22
申请号：US627301
申请日：1996-04-03
申请人： Jerome Shen , Mark A. Roussey , Barbara A. Bryan , Maryann C. Allred
发明人： Jerome Shen , Mark A. Roussey , Barbara A. Bryan , Maryann C. Allred
IPC分类号： A23J3/14 , A23J3/16 , A23L11/00 , A23L11/30 , C12P17/06 , C12P21/06 , C12P19/62
CPC分类号： A23J3/16 , C12P17/06
摘要： An aglucone isoflavone enriched vegetable protein whey, whey protein material, high genistein material, high daidzein material, and aglucone isoflavone material are provided, as well as a process for producing the same from a vegetable protein whey. Isoflavone conjugates in a vegetable protein whey are converted to isoflavone glucosides by treating the whey at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glucosides are converted to aglucone isoflavones by enzymatic reaction to produce an aglucone isoflavone enriched vegetable protein whey. Aglucone isoflavone whey protein material is recovered from the aglucone isoflavone enriched vegetable protein whey. A high genistein content material, a high daidzein content material, and an aglucone isoflavone material are produced from an alcohol extract of the aglucone isoflavone whey protein material.
摘要翻译：提供了富含配基异黄酮的植物蛋白乳清，乳清蛋白质材料，高染料木黄酮材料，高黄豆苷原物质和配基异黄酮材料，以及由植物蛋白乳清生产的方法。将植物蛋白乳清中的异黄酮共轭物通过在温度和pH下处理乳清一段时间以转化为异黄酮糖苷而转化为异黄酮糖苷。通过酶促反应将异黄酮糖苷转化成配基异黄酮，生成富含配基异黄酮的植物蛋白乳清。从配基异黄酮植物蛋白乳清中回收葡萄糖异黄酮乳清蛋白物质。高分子量染料木素含量材料，高黄豆苷元含量物质和配基异黄酮材料由配基异黄酮乳清蛋白材料的醇提取物生产。

4. 发明授权

US5763389A Aglucone isoflavone enriched vegetable protein extract and isolate and process for producing 失效
标题翻译：葡聚糖异黄酮富集植物蛋白提取物，分离和加工生产
公开(公告)号：US5763389A
公开(公告)日：1998-06-09
申请号：US307752
申请日：1996-04-12
申请人： Jerome L. Shen , Barbara A. Bryan
发明人： Jerome L. Shen , Barbara A. Bryan
IPC分类号： C07K1/30 , A23J3/14 , A23J3/34 , C07D311/36 , C07K1/02 , C07K1/14 , C07K1/36 , C07K14/415 , C12P17/06 , A61K31/35 , A61K38/02
CPC分类号： C12P17/06 , C07K14/415
摘要： Aglucone isoflavone enriched vegetable protein extract and isolate and processes for producing and recovering are disclosed. The aglucone isoflavone extract is made by extracting a vegetable protein material comprising glucone isoflavones with an aqueous extractant having a pH above about the isoelectric point of the protein material to produce an aqueous extract, and reacting the glucone isoflavones with a sufficient amount of beta-glucosidase enzyme or esterase enzyme for a time period, temperature, and pH sufficient to convert at least a majority of the glucone isoflavones in the extract to aglucone isoflavones and thereby produce the aglucone isoflavone enriched extract. The aglucone isoflavone enriched isolates are produced by adjusting the pH of the reacted extract to about the isoelectric point of the vegetable protein material to precipitate the protein material, and separating the protein material to produce an aglucone enriched protein isolate.
摘要翻译： PCT No.PCT / US94 / 10697 Sec。 371日期：1996年4月12日 102（e）日期1996年4月12日PCT 1994年9月21日PCT PCT。公开号WO95 / 10530 日期：1995年4月20日公开了富含异黄酮的植物蛋白提取物和分离物及其制备和回收方法。配基异黄酮提取物是通过用pH高于蛋白质材料的等电点的水提取剂提取含有葡萄糖异黄酮的植物蛋白质材料制成的，以产生水提取物，并使葡萄糖异黄酮与足够量的β-葡糖苷酶反应酶或酯酶一段时间，温度和pH足以将提取物中的至少大部分葡萄糖异黄酮转化成配基异黄酮，从而产生富含配基异黄酮的提取物。通过将反应的提取物的pH调节到植物蛋白材料的等电点以产生蛋白质材料，并分离蛋白质材料以产生富含葡萄糖的蛋白质分离物，产生富含异黄酮的分离物。

5. 发明授权

US4933281A Method for producing rhamnose 失效
标题翻译：生产鼠李糖的方法
公开(公告)号：US4933281A
公开(公告)日：1990-06-12
申请号：US26857
申请日：1987-03-17
申请人： Lacy Daniels , Robert J. Linhardt , Barbara A. Bryan , Friedrich Mayerl , Wilhelm Pickenhagen
发明人： Lacy Daniels , Robert J. Linhardt , Barbara A. Bryan , Friedrich Mayerl , Wilhelm Pickenhagen
IPC分类号： C12N1/20 , C07C59/01 , C07H13/04 , C12P7/42 , C12P19/02 , C12P19/44 , C12R1/385
CPC分类号： C07H13/04 , C07C59/01 , C12P19/44 , Y10S435/822 , Y10S435/874 , Y10S435/875
摘要： A method for large-scale production of rhamnose and 3-hydroxydecanoic acid is disclosed comprising the steps of growing microorganisms of Pseudomonas sp. capable of production of high levels of rhamnolipid in a defined culture medium containing vegetable oil. Additional steps include isolating the rhamnolipid from the culture medium, hydrolyzing the rhamnolipid to produce rhamnose and 3-hydroxydecanoic acid, and separating the rhamnose from the acid. Corn oil is the preferred vegetable oil and Pseudomonas aeruginosa is the preferred Pseudomonas sp. Non-limiting concentrations of nitrogen compounds and magnesium compounds and limiting concentrations of iron compounds are additionally preferably included in the culture medium.

6. 发明授权

US06521282B1 Aglucone isoflavone enriched vegetable flour and grit 失效
标题翻译：富含葡萄糖的异黄酮植物粉和砂砾
公开(公告)号：US06521282B1
公开(公告)日：2003-02-18
申请号：US09702265
申请日：2000-10-31
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： A23L120
CPC分类号： A23L11/33
摘要： A vegetable protein flour or grit is provided which is rich in aglucone isoflavones. In one embodiment, the vegetable protein flour or grit contains at least 400 parts per million of genistein and 400 ppm of daidzein. In another embodiment the vegetable protein flour or grit contains at least 50% genistein and daidzein relative to all genistein family isoflavones and daidzein family isoflavones, respectively.
摘要翻译：提供富含配基异黄酮的植物蛋白粉或粗粒。在一个实施方案中，植物蛋白粉或砂砾含有至少400ppm的染料木黄酮和400ppm的黄豆苷原。在另一个实施方案中，相对于所有染料木素族异黄酮和大豆黄素家族异黄酮，植物蛋白粉或砂砾分别含有至少50％的染料木黄酮和黄豆苷原。

7. 发明授权

US06495141B2 Recovery of isoflavones from soy molasses 有权
标题翻译：从大豆糖蜜中回收异黄酮
公开(公告)号：US06495141B2
公开(公告)日：2002-12-17
申请号：US10016461
申请日：2001-10-19
申请人： Doyle H. Waggle , Barbara A. Bryan
发明人： Doyle H. Waggle , Barbara A. Bryan
IPC分类号： A01N6500
CPC分类号： C07D311/40 , C07D311/36 , C07H17/07
摘要： Isoflavone compositions derived from soy molasses are provided. In a first embodiment, an isoflavone material derived from soy molasses containing at least two isoflavones is provided, where at least one of the isoflavones is genistein. In a second embodiment, an isoflavone material derived from soy molasses containing at least two isoflavones is provided, where at least one of the isoflavones is daidzein.
摘要翻译：提供衍生自大豆糖蜜的异黄酮组合物。在第一实施方案中，提供衍生自含有至少两种异黄酮的大豆糖蜜的异黄酮物质，其中至少一种异黄酮是染料木黄酮。在第二实施方案中，提供衍生自含有至少两种异黄酮的大豆糖蜜的异黄酮物质，其中至少一种异黄酮是黄豆苷元。

8. 发明授权

US5726034A Aglucone isoflavone enriched vegetable protein extract and protein material, and high genistein and daidzein content materials and process for producing the same 失效
标题翻译：富含肌苷异黄酮的植物蛋白提取物和蛋白质材料，高染料木黄酮和黄豆苷原含量的材料及其制备方法
公开(公告)号：US5726034A
公开(公告)日：1998-03-10
申请号：US709026
申请日：1996-09-06
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： C12P17/16 , A23J3/14 , A23J3/16 , A23L11/00 , A23L11/30 , A61K31/35 , A61K31/352 , C07D311/36 , C07K14/415 , C12P17/06 , C21P21/06 , A61K35/78 , C12N9/24 , A61K31/337
CPC分类号： C12P17/06 , A23J3/14 , A23J3/16 , A23L11/07 , A23L11/33 , A23L11/34
摘要： An aglucone is isoflavone enriched vegetable protein extract and protein material are provided, as well as a high genistein content material and a high daidzein content material. Isoflavone conjugates in a vegetable material are converted to isoflavone glucosides by treating the vegetable material at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glycosides are converted to glucose isoflavones by enzymatic reaction. The vegetable material is extracted with an aqueous extractant having a pH above about the isoelectric point of protein in the vegetable material to extract protein and the isoflavones either before or after conversion of the isoflavone conjugates to isoflavone glucosides or the conversion of the isoflavone glucosides to aglucone isoflavones. An aglucone isoflavone enriched protein material is produced by precipitating the protein and aglucone isoflavones from the extract. A high genistein content material or a high daidzein content material may be produced from the aglucone isoflavone enriched protein extract or aglucone isoflavone enriched protein material by separating the high genistein or high daidzein content material from the extract or protein material.
摘要翻译：配基是富含异黄酮的植物蛋白提取物和蛋白质材料，以及高染料木黄酮含量的材料和高黄豆苷原含量的材料。将植物材料中的异黄酮共轭物通过在温度和pH下处理植物材料一段足以实现转化的时间转化为异黄酮糖苷。异黄酮糖苷通过酶反应转化成葡萄糖异黄酮。植物材料用pH高于植物原料蛋白质等电点的水提取剂提取，以在将异黄酮共轭物转化为异黄酮糖苷之前或之后提取蛋白质和异黄酮，或将异黄酮糖苷转化成配基异黄酮通过从提取物中沉淀蛋白质和配基异黄酮产生富含配基异黄酮的蛋白质材料。通过从提取物或蛋白质材料中分离高染料木黄酮或高黄豆苷原含量的材料，可以从富含配基异黄酮的蛋白质提取物或富含配基异黄酮的蛋白质材料中产生高染料木黄酮含量的材料或高黄豆苷原含量的材料。

9. 发明授权

US5637561A Aglucone isoflavone enriched vegetable protein whey, whey protein, and process for producing 失效
标题翻译：富含葡聚糖的异黄酮植物蛋白乳清，乳清蛋白及其制备方法
公开(公告)号：US5637561A
公开(公告)日：1997-06-10
申请号：US307731
申请日：1996-04-12
申请人： Jerome L. Shen , Barbara A. Bryan
发明人： Jerome L. Shen , Barbara A. Bryan
IPC分类号： A23J3/14 , A23J3/34 , A23L33/00 , C07D311/36 , C07K1/14 , C07K1/30 , C07K1/34 , C07K14/415 , C12P17/06 , C12P21/02 , A61K31/335 , A61K38/02
CPC分类号： C12P17/06 , C07D311/36
摘要： Aglucone isoflavone enriched vegetable protein whey, whey protein and processes for producing and recovering such are disclosed. Aglucone isoflavone enriched vegetable protein whey is made by treating whey comprising glucone isoflavones with a sufficient amount of beta-glucosidase enzyme or esterase enzyme or acid to convert at least a majority of the glucone isoflavones to aglucones and thereby provide an aglucone enriched whey. An aglucone enriched whey protein is obtained by recovery of the protein.
摘要翻译： PCT No.PCT / US94 / 10699 Sec。 371日期：1996年4月12日 102（e）日期1996年4月12日PCT 1994年9月21日PCT PCT。第WO95 / 10512号公报日期1995年04月20日公开了富含葡萄糖的异黄酮植物蛋白乳清，乳清蛋白及其生产和回收方法。通过用足够量的β-葡糖苷酶或酯酶或酸处理包含葡糖异黄酮的乳清来将至少大部分的葡萄糖异黄酮转化为甙元，从而提供富含配基的乳清，从而制备富含异亮氨酸的富含异黄酮的植物蛋白乳清。通过回收蛋白质获得富集配基的乳清蛋白。

10. 发明授权

US06703051B1 Process for separating and recovering protein and isoflavones from a plant material 失效
标题翻译：从植物材料中分离和回收蛋白质和异黄酮的方法
公开(公告)号：US06703051B1
公开(公告)日：2004-03-09
申请号：US09170514
申请日：1998-10-13
申请人： Gregory A. Bates , Barbara A. Bryan
发明人： Gregory A. Bates , Barbara A. Bryan
IPC分类号： A61K3578
CPC分类号： A23J1/14 , A23L33/105
摘要： A method is provided for separating and recovering protein and isoflavones from a plant material. A clarified protein extract of the plant material is prepared and contacted with a polar ion exchange resin, such as an anion exchange resin allowing the isoflavones in the extract to bind with the ion exchange resin and depleting the extract of isoflavones. A protein extract which has been depleted of isoflavones is separated from the ion exchange resin, and an isoflavone depleted protein material is recovered from the isoflavone depleted extract. After removal of the isoflavone depleted extract from the resin, the isoflavones are separated and recovered from the ion exchange resin. Recovered isoflavone glycosides and isoflavone glycoside conjugates may be converted to their corresponding aglucone isoflavone form.
摘要翻译：提供了从植物材料中分离和回收蛋白质和异黄酮的方法。制备植物材料的澄清蛋白质提取物并与极性离子交换树脂例如阴离子交换树脂接触，使得提取物中的异黄酮与离子交换树脂结合并消耗异黄酮的提取物。将已经耗尽异黄酮的蛋白质提取物与离子交换树脂分离，并从异黄酮消耗的提取物中回收异黄酮消耗的蛋白质材料。从树脂中除去异黄酮消耗的提取物后，从离子交换树脂中分离并回收异黄酮。回收的异黄酮糖苷和异黄酮糖苷共轭物可以转化成其相应的配基异黄酮形式。

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