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1. 发明授权

US09310362B2 Microwell array chip and method of manufacturing same 有权
标题翻译：微孔阵列芯片及其制造方法相同
公开(公告)号：US09310362B2
公开(公告)日：2016-04-12
申请号：US13088582
申请日：2011-04-18
申请人： Atsushi Muraguchi , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
发明人： Atsushi Muraguchi , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
IPC分类号： C12N5/16 , C12N5/00 , G01N33/543 , B01J19/00 , A61K35/12 , A61K48/00
CPC分类号： G01N33/54366 , A61K35/12 , A61K48/00 , B01J19/0046 , B01J2219/00659 , C12M23/12 , G06F19/00
摘要： A microwell array chip made of silicon and having multiple microwells where each microwell is used to store a single specimen organic cell. The microwell of the array chip is of a size and shape to hold just one organic cell and the interior surface of the microwells are coated with a fluorocarbon film.
摘要翻译：由硅制成并具有多个微孔的微孔阵列芯片，其中每个微孔用于存储单个样品有机电池。阵列芯片的微孔具有仅容纳一个有机电池的尺寸和形状，并且微孔的内表面涂覆有氟碳膜。

2. 发明申请

US20080014631A1 Microwell Array Chip and Its Manufacturing Method 审中-公开
标题翻译：微孔阵列芯片及其制造方法
公开(公告)号：US20080014631A1
公开(公告)日：2008-01-17
申请号：US10573289
申请日：2004-09-22
申请人： Atsushi Muraguchi , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
发明人： Atsushi Muraguchi , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
IPC分类号： C12M1/34
CPC分类号： G01N33/54366 , A61K35/12 , A61K48/00 , B01J19/0046 , B01J2219/00659 , C12M23/12
摘要： A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein microwell markers are present on the same substrate surface as the openings of the microwells. A microwell array chip having multiple microwells on a principal surface of a substrate, said microwells being of a shape and size permitting the storage of only a single organic cell in each microwell, wherein protrusions are present in the openings of said microwells so as to narrow said openings. A method for manufacturing the microwell array chip. The method comprises the steps of: forming a film on at least one principal surface of a substrate; applying a resist coating on the film that has been formed; exposing the resist surface through a mask having a microwell pattern and removing uncured portions of resist; etching the exposed portions of said film and substrate to fabricate wells in the form of a microwell array; and removing the resist. A microwell array chip made of silicon and having multiple microwells, each microwell being used to store a single specimen organic cell, wherein each microwell is of a size and shape holding just one organic cell.
摘要翻译：一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中微孔标记物存在于与基底表面的开口相同的基底表面上微孔。一种微孔阵列芯片，其在基底的主表面上具有多个微孔，所述微孔具有允许在每个微孔中仅存储单个有机细胞的形状和尺寸，其中突起存在于所述微孔的开口中以使其变窄说开口一种微孔阵列芯片的制造方法。该方法包括以下步骤：在衬底的至少一个主表面上形成膜; 在已经形成的膜上施加抗蚀剂涂层; 通过具有微孔图案的掩模曝光抗蚀剂表面并除去未固化的抗蚀剂部分; 蚀刻所述膜和基底的暴露部分以制造微孔阵列形式的孔; 并除去抗蚀剂。由硅制成并具有多个微孔的微孔阵列芯片，每个微孔用于存储单个样品有机电池，其中每个微孔具有仅具有一个有机电池的尺寸和形状。

3. 发明申请

US20110195496A1 MICROWELL ARRAY CHIP AND METHOD OF MANUFACTURING SAME 有权
标题翻译： MICROWELL阵列芯片及其制造方法
公开(公告)号：US20110195496A1
公开(公告)日：2011-08-11
申请号：US13088582
申请日：2011-04-18
申请人： Atsushi MURAGUCHI , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
发明人： Atsushi MURAGUCHI , Hiroyuki Kishi , Yoshiharu Tokimitsu , Sachiko Kondo , Tsutomu Obata , Satoshi Fujiki , Yoshiyuki Yokoyama , Hirofumi Nabesawa , Sotohiro Takabayashi , Katsumi Tanino
IPC分类号： C12N5/078
CPC分类号： G01N33/54366 , A61K35/12 , A61K48/00 , B01J19/0046 , B01J2219/00659 , C12M23/12 , G06F19/00
摘要： The present invention is directed to a microwell array chip made of silicon and having multiple microwells. Each microwell is used to store a single specimen organic cell. Each microwell is of a size and shape holding just one organic cell, and the interior surface of the microwells are coated with a fluorocarbon film.
摘要翻译：本发明涉及由硅制成并具有多个微孔的微孔阵列芯片。每个微孔用于存储单个样品有机细胞。每个微孔具有只容纳一个有机电池的尺寸和形状，并且微孔的内表面涂覆有氟碳膜。

4. 发明授权

US09494575B2 Cells screening method 有权
标题翻译：细胞筛选方法
公开(公告)号：US09494575B2
公开(公告)日：2016-11-15
申请号：US12671690
申请日：2008-08-01
申请人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
发明人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
IPC分类号： C40B30/04 , C12N5/0781 , C40B60/12 , G01N33/50 , C12N5/10 , C12N5/071 , C12N5/16
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip. A microwell array comprises multiple wells capturing single cells and a coating layer on a principal surface around the wells containing a substance capable of binding to a substance produced by the cells in the wells. A method for screening a target cell comprises: causing specimen cells and a cell culture broth to be contained in the wells of the microwell array; culturing the cells in a state permitting the diffusion of substances from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell onto the coating layer; and detecting the substance produced by the target cell by the label substance binding around the well to specify the target cell.
摘要翻译：提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性质的方法和手段。微孔阵列包括捕获单个细胞的多个孔和围绕孔的主表面上的包覆层，其包含能够结合孔中由细胞产生的物质的物质。用于筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在微孔阵列的孔中; 以允许物质从孔扩散到涂层中的状态培养细胞; 将与靶细胞产生的物质特异性结合的标签物质进料到涂层上; 以及通过在井附近结合的标记物质来检测由靶细胞产生的物质以指定靶细胞。

5. 发明申请

US20110294678A1 CELLS SCREENING METHOD 有权
标题翻译：细胞筛选方法
公开(公告)号：US20110294678A1
公开(公告)日：2011-12-01
申请号：US12671690
申请日：2008-08-01
申请人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
发明人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
IPC分类号： C40B30/04 , C12N5/0781 , C12N5/10 , C12N5/071 , C12N5/0783 , C40B60/12 , C12N5/16
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.
摘要翻译：提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性的方法和手段，以及单独测定各细胞的状态。微孔阵列包括多个孔和在基底构件的一个主表面上的涂层，所述孔具有允许仅将单个细胞进入每个孔的尺寸。能够与孔中包含的细胞产生的物质结合的物质的涂层存在于孔周围的主表面上。筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在上述微孔阵列的孔中; 将涂层和孔浸入培养液中，并使培养液中的物质从孔扩散到涂层中的状态下培养细胞; 将标本物质特异性地与存在于试样细胞中的靶细胞产生的物质结合到涂层上; 以及通过所述标记物质检测由所述靶层细胞产生的与所述涂层中的物质结合的物质，以指定所述靶细胞。

6. 发明授权

US10525281B2 Spacer for ionized radiation therapy 有权
公开(公告)号：US10525281B2
公开(公告)日：2020-01-07
申请号：US13504989
申请日：2010-10-28
申请人： Takumi Fukumoto , Tsutomu Obata , Yoshitaka Tagami , Eiichi Uemura , Michiko Manabe
发明人： Takumi Fukumoto , Tsutomu Obata , Yoshitaka Tagami , Eiichi Uemura , Michiko Manabe
IPC分类号： A61N5/10 , G21F3/00 , G21F1/10 , A61B90/00
摘要： Provided is a spacer for ionizing radiation therapy, which can be easily disposed in a living body, does not require abdominal surgery for removing the disposed spacer after radiation therapy, and can effectively separate a tissue to be treated from other tissues. The spacer for ionizing radiation therapy includes a fiber assembly obtained by three-dimensionally entangling a fibrous material formed of a biocompatible synthetic polymeric material. Specifically, the spacer has a thickness of 1 mm to 100 mm.

7. 发明申请

US20120271093A1 Spacer For Ionized Radiation Therapy 审中-公开
标题翻译：用于电离辐射治疗的间隔物
公开(公告)号：US20120271093A1
公开(公告)日：2012-10-25
申请号：US13504989
申请日：2010-10-28
申请人： Takumi Fukumoto , Tsutomu Obata , Yoshitaka Tagami , Eiichi Uemura , Michiko Manabe
发明人： Takumi Fukumoto , Tsutomu Obata , Yoshitaka Tagami , Eiichi Uemura , Michiko Manabe
IPC分类号： A61N5/10
CPC分类号： A61N5/10 , A61B2090/0445 , A61B2090/0815 , A61N2005/1094 , A61N2005/1096 , G21F1/10 , G21F3/00
摘要： Provided is a spacer for ionizing radiation therapy, which can be easily disposed in a living body, does not require abdominal surgery for removing the disposed spacer after radiation therapy, and can effectively separate a tissue to be treated from other tissues. The spacer for ionizing radiation therapy includes a fiber assembly obtained by three-dimensionally entangling a fibrous material formed of a biocompatible synthetic polymeric material. Specifically, the spacer has a thickness of 1 mm to 100 mm.
摘要翻译：本发明提供一种电离辐射治疗用间隔物，其可以容易地设置在生物体内，不需要腹部手术去除放射线治疗后的配置间隔物，能够有效地将待治疗的组织与其他组织分离。用于电离辐射治疗的间隔物包括通过三维缠绕由生物相容性合成聚合物材料形成的纤维材料获得的纤维组件。具体而言，间隔物的厚度为1mm〜100mm。

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