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1. 发明授权

US09494575B2 Cells screening method 有权
标题翻译：细胞筛选方法
公开(公告)号：US09494575B2
公开(公告)日：2016-11-15
申请号：US12671690
申请日：2008-08-01
申请人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
发明人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
IPC分类号： C40B30/04 , C12N5/0781 , C40B60/12 , G01N33/50 , C12N5/10 , C12N5/071 , C12N5/16
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip. A microwell array comprises multiple wells capturing single cells and a coating layer on a principal surface around the wells containing a substance capable of binding to a substance produced by the cells in the wells. A method for screening a target cell comprises: causing specimen cells and a cell culture broth to be contained in the wells of the microwell array; culturing the cells in a state permitting the diffusion of substances from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell onto the coating layer; and detecting the substance produced by the target cell by the label substance binding around the well to specify the target cell.
摘要翻译：提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性质的方法和手段。微孔阵列包括捕获单个细胞的多个孔和围绕孔的主表面上的包覆层，其包含能够结合孔中由细胞产生的物质的物质。用于筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在微孔阵列的孔中; 以允许物质从孔扩散到涂层中的状态培养细胞; 将与靶细胞产生的物质特异性结合的标签物质进料到涂层上; 以及通过在井附近结合的标记物质来检测由靶细胞产生的物质以指定靶细胞。

2. 发明申请

US20110294678A1 CELLS SCREENING METHOD 有权
标题翻译：细胞筛选方法
公开(公告)号：US20110294678A1
公开(公告)日：2011-12-01
申请号：US12671690
申请日：2008-08-01
申请人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
发明人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
IPC分类号： C40B30/04 , C12N5/0781 , C12N5/10 , C12N5/071 , C12N5/0783 , C40B60/12 , C12N5/16
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.
摘要翻译：提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性的方法和手段，以及单独测定各细胞的状态。微孔阵列包括多个孔和在基底构件的一个主表面上的涂层，所述孔具有允许仅将单个细胞进入每个孔的尺寸。能够与孔中包含的细胞产生的物质结合的物质的涂层存在于孔周围的主表面上。筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在上述微孔阵列的孔中; 将涂层和孔浸入培养液中，并使培养液中的物质从孔扩散到涂层中的状态下培养细胞; 将标本物质特异性地与存在于试样细胞中的靶细胞产生的物质结合到涂层上; 以及通过所述标记物质检测由所述靶层细胞产生的与所述涂层中的物质结合的物质，以指定所述靶细胞。

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