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1. 发明授权

US10031142B2 In situ chemiluminescent substrates and assays 有权
公开(公告)号：US10031142B2
公开(公告)日：2018-07-24
申请号：US13808922
申请日：2011-07-06
申请人： Alison Sparks , Zhixian Wang , Melissa Gee
发明人： Alison Sparks , Zhixian Wang , Melissa Gee
IPC分类号： G01N33/58 , C12Q1/42 , C12Q1/34 , C07F9/12 , C07F9/40 , C07F9/6541 , C07F9/655 , C07F9/6558 , C07H15/203 , C12Q1/6816 , C07D321/00
摘要： Methods for generating a chemiluminescent enzyme substrate in situ, in aqueous or other assay conditions. Also disclosed are methods to use the substrates to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Kits relating to these methods are also disclosed.

2. 发明申请

US20130196325A1 In Situ Chemiluminescent Substrates and Assays 审中-公开
标题翻译：原位化学发光底物和测定
公开(公告)号：US20130196325A1
公开(公告)日：2013-08-01
申请号：US13808922
申请日：2011-07-06
申请人： Alison Sparks , Zhixian Wang , Melissa Gee
发明人： Alison Sparks , Zhixian Wang , Melissa Gee
IPC分类号： G01N33/58 , C12Q1/42 , C12Q1/68 , C12Q1/34
CPC分类号： G01N33/581 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/6541 , C07F9/65512 , C07F9/65583 , C07H15/203 , C12Q1/34 , C12Q1/42 , C12Q1/6816
摘要： Methods for generating a chemiluminescent enzyme substrate in situ, in aqueous or other assay conditions. Also disclosed are methods to use the substrates to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Kits relating to these methods are also disclosed.
摘要翻译：在水性或其他测定条件下原位生成化学发光酶底物的方法。还公开了使用底物产生光，检测和/或定量酶，抗原和/或核酸的方法。还披露了与这些方法有关的套件。

3. 发明申请

US20110003277A1 Dioxetane-Nanoparticle Assemblies For Energy Transfer Detection Systems, Methods Of Making The Assemblies, And Methods Of Using The Assemblies in Bioassays 审中-公开
标题翻译：用于能量转移检测系统的二氧杂环丁烷 - 纳米粒子组件，制造组件的方法以及在生物测定中使用组件的方法
公开(公告)号：US20110003277A1
公开(公告)日：2011-01-06
申请号：US12819844
申请日：2010-06-21
申请人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
发明人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C12Q1/00 , H01B1/22
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , G01N33/581
摘要： Assemblies comprising nanoparticles and chemiluminescent substrates such as dioxetanes are provided. The assemblies can be used in assays to detect the presence and/or amount of a single analyte or multiple analytes in a sample. Methods of making the assemblies are also described.
摘要翻译：提供了包含纳米颗粒和化学发光底物如二氧杂环丁烷的组件。组合物可用于测定以检测样品中单一分析物或多个分析物的存在和/或量。还描述了制造组件的方法。

4. 发明申请

US20060216768A1 Dioxetane-nanoparticle assemblies for energy transfer detection systems, methods of making the assemblies, and methods of using the assemblies in bioassays 审中-公开
标题翻译：用于能量转移检测系统的二氧杂环丁烷 - 纳米颗粒组件，制造组件的方法和在生物测定中使用组件的方法
公开(公告)号：US20060216768A1
公开(公告)日：2006-09-28
申请号：US11221895
申请日：2005-09-09
申请人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
发明人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
IPC分类号： G01N33/533
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , G01N33/581
摘要： Assemblies comprising nanoparticles and chemiluminescent substrates such as dioxetanes are provided. The assemblies can be used in assays to detect the presence and/or amount of a single analyte or multiple analytes in a sample. Methods of making the assemblies are also described.
摘要翻译：提供了包含纳米颗粒和化学发光底物如二氧杂环丁烷的组件。组合物可用于测定以检测样品中单一分析物或多个分析物的存在和/或量。还描述了制造组件的方法。

5. 发明授权

US6140495A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6140495A
公开(公告)日：2000-10-31
申请号：US296539
申请日：1999-04-22
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07D327/06 , C07F9/02 , C07F9/06 , C07F9/28 , C07H19/04
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T + E，1/2 + EE动力学以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变，在芳族取代基上除了与二氧杂环丁烷的连接点相邻的位置之外的位置。信号强度可以通过认可的化学发光增强剂进一步增强。

6. 发明授权

US5843681A Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore 失效
标题翻译：因此，用于化学发光检测蛋白酶的二氧杂环丁烷化合物，使用方法和试剂盒
公开(公告)号：US5843681A
公开(公告)日：1998-12-01
申请号：US728990
申请日：1996-10-11
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
IPC分类号： G01N33/573 , C07D321/00 , C07K5/072 , C07K5/08 , C07K5/09 , C07K5/10 , C12Q1/37 , G01N33/58
CPC分类号： C07K5/06095 , C07D321/00 , C07K5/0802 , C07K5/0817 , C07K5/1002 , C12Q1/37 , G01N33/582 , Y10S530/807
摘要： 1, 2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
摘要翻译：提供了带有蛋白水解酶特异性氨基酸或肽的1,2-二氧杂环丁烷化合物，该氨基酸或肽可以通过相应的蛋白酶的作用除去。当去除氨基酸或肽时，1,2-二氧杂环丁烷以化学发光分解，产生光，提供快速，超灵敏和方便的手段，用于检测待检样品中蛋白酶的存在。产生的光量或化学发光度可以与存在的蛋白酶的量相关。提供免疫测定，以及DNA杂交和DNA探针测定。

7. 发明授权

US5591591A Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore 失效
标题翻译：用于化学发光检测蛋白酶的二氧杂环丁烷化合物，使用方法及其试剂盒
公开(公告)号：US5591591A
公开(公告)日：1997-01-07
申请号：US385788
申请日：1995-02-09
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
IPC分类号： G01N33/573 , C07D321/00 , C07K5/072 , C07K5/08 , C07K5/09 , C07K5/10 , C12Q1/37 , G01N33/58 , C07K5/06
CPC分类号： C07K5/06095 , C07D321/00 , C07K5/0802 , C07K5/0817 , C07K5/1002 , C12Q1/37 , G01N33/582 , Y10S530/807
摘要： 1,2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
摘要翻译：提供了带有蛋白水解酶特异性氨基酸或肽的1,2-二氧环乙烷化合物，该氨基酸或肽可以通过相应的蛋白酶的作用除去。当去除氨基酸或肽时，1,2-二氧杂环丁烷以化学发光分解，产生光，提供快速，超灵敏和方便的手段，用于检测待检样品中蛋白酶的存在。产生的光量或化学发光度可以与存在的蛋白酶的量相关。提供免疫测定，以及DNA杂交和DNA探针测定。

8. 发明授权

US6022964A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6022964A
公开(公告)日：2000-02-08
申请号：US904847
申请日：1997-08-01
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07D327/06 , C07F9/02 , C07F9/06 , C07F9/28 , C07H19/04
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T1 / 2动力学，以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变芳香族取代基上的位置比邻近二氧杂环丁烷的点附近的那些。信号强度可以通过认可的化学发光增强剂进一步增强。

9. 发明授权

US5783381A Chemiluminescent 1,2-dioxetanes 失效
公开(公告)号：US5783381A
公开(公告)日：1998-07-21
申请号：US545174
申请日：1995-10-19
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
IPC分类号： G01N31/00 , C07D305/06 , C07D321/00 , C07F7/18 , C09K11/07 , C12Q1/34 , G01N21/78 , G01N33/52 , C12Q1/00
CPC分类号： C12Q1/34 , C07D321/00 , C07F7/1856 , G01N33/52 , Y10S435/975
摘要： Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence of a base released by physical or natural processes, to calibrate light measuring apparatus and to determine the amount of reducing or oxidizing agent present in the base.

10. 发明申请

US20060079699A1 Intermediate compounds and methods for synthesizing chemiluminescent dioxetane substrates 审中-公开
标题翻译：中间体化合物和合成化学发光二恶环丁烷底物的方法
公开(公告)号：US20060079699A1
公开(公告)日：2006-04-13
申请号：US11210967
申请日：2005-08-25
申请人： Brooks Edwards , Rouh-Rong Juo , Alison Sparks
发明人： Brooks Edwards , Rouh-Rong Juo , Alison Sparks
IPC分类号： C07F9/655
CPC分类号： C07F9/6551 , C07F9/12
摘要： Methods for synthesizing 1,2-dioxetane compounds and intermediate compounds useful in the synthesis of these dioxetane compounds are described. The methods and intermediates allow for the efficient production of dioxetane substrates having electron withdrawing groups via a Homer Emmons coupling route.
摘要翻译：描述了用于合成这些二氧杂环丁烷化合物的1,2-二氧环乙烷化合物和中间体化合物的合成方法。方法和中间体允许通过荷马·埃蒙斯（Homer Emmons）偶联途径有效地制备具有吸电子基团的二氧杂环丁烷底物。

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