专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5242792A Method for the preservation of red blood cells by lyophilization using glycerol or inositol with disaccharides 失效
标题翻译：使用甘油或肌醇与二糖冷冻干燥保存红细胞的方法
公开(公告)号：US5242792A
公开(公告)日：1993-09-07
申请号：US659765
申请日：1991-02-25
申请人： Alan S. Rudolph , Joseph P. Larry
发明人： Alan S. Rudolph , Joseph P. Larry
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0221
摘要： The invention is directed to a composition comprising a permeabilizing agent, a preserving agent, and a buffered solvent. This composition is used to prepare the cells for lyophilization cells and to rehydrate the cells to recover them from lyophilization.The process of this invention comprises adding the permeabilizing agent and the preserving agent in a buffered solution to red blood cells, agitating the combination for a period of time sufficient to allow permeation of the preserving agent into the cell, shell freezing the mixture, and lyophilizing the mixture. The dry lyophilized material can then be stored. The cells can be rehydrated using the same composition of permeability agent, preserving agent and buffered solvent.
摘要翻译：本发明涉及包含透化剂，防腐剂和缓冲溶剂的组合物。该组合物用于制备用于冻干细胞的细胞并将细胞再水化以从冷冻干燥中回收它们。本发明的方法包括将缓释溶液中的透化剂和防腐剂加入到红细胞中，搅拌该组合一段足以允许保存剂渗入细胞的时间，壳冷冻该混合物，并冻干混合物。然后可以将干燥的冻干材料储存。可以使用相同组成的渗透剂，防腐剂和缓冲溶剂来再水化细胞。

2. 发明授权

US5464629A Method of forming hydrogel particles having a controlled size using liposomes 失效
标题翻译：使用脂质体形成具有受控尺寸的水凝胶颗粒的方法
公开(公告)号：US5464629A
公开(公告)日：1995-11-07
申请号：US152366
申请日：1993-11-16
申请人： Mariam Monshipouri , Alan S. Rudolph
发明人： Mariam Monshipouri , Alan S. Rudolph
IPC分类号： A61K9/127 , A61K47/48 , B01J2/00 , B01J2/06 , B01J13/02 , B01J13/04
CPC分类号： A61K47/48784 , A61K47/48876 , A61K47/48923 , A61K9/127 , B01J13/02 , B01J13/04 , B01J2/003 , B01J2/06 , Y10T428/2984
摘要： A method of fabricating hydrogel particles of controlled size within liposomes is provided, which entails encapsulating an effective amount of one or more hydrogel-forming substances in liposomes in a liquid medium, removing any unencapsulated hydrogel-forming substances from the liquid medium, adding initiator to the liquid medium and into the liposomes, thereby initiating reaction of the one or more hydrogel-forming substances, and removing any extra-liposomal initiators from the medium.
摘要翻译：提供了一种在脂质体内制备受控尺寸的水凝胶颗粒的方法，其包括在液体介质中将有效量的一种或多种水凝胶形成物质包封在脂质体中，从液体介质中除去任何未包封的水凝胶形成物质，将引发剂加入到液体介质并进入脂质体，从而引发一种或多种形成水凝胶的物质的反应，并从介质中除去任何超脂质体引发剂。

3. 发明授权

US5051353A Preservation and restoration of hemoglobin in blood substitutes 失效
标题翻译：血液代用品中血红蛋白的保存和恢复
公开(公告)号：US5051353A
公开(公告)日：1991-09-24
申请号：US233303
申请日：1988-08-09
申请人： Lewis P. Stratton , Martha C. Farmer , Alan S. Rudolph
发明人： Lewis P. Stratton , Martha C. Farmer , Alan S. Rudolph
IPC分类号： A61K38/42
CPC分类号： A61K38/42
摘要： A hemoglobin-containing blood substitute preparation is protected from oxtion to methemoglobin. The protected preparation comprises between 10 mM and 60 mM of a compound selected from the group consisting of glutathione (GSH), nicotinamide adenine dinucleotide (NADH), nicotine adenine dinucleotide phosphate (NADPH) and mixtures of these in the aqueous phase of a hemoglobin-containing fluid. Oxidized hemoglobin in a preparation can be restored by adding between 10 mM and 60 mM of a compound selected from the group consisting of glutathione (GSH), nicotinamide adenine dinucleotide (NADH), nicotine adenine dinucleotide phosphate (NADPH) and mixtures of these to a hemoglobin-containing fluid in which the hemoglobin has converted in whole or part to methemoglobin, and storing the fluid at storage temperatures for blood products above the freezing point of the fluid for a time sufficient to convert methemoglobin to hemoglobin.
摘要翻译：含血红蛋白的血液替代物制剂被保护免于氧化成高铁血红蛋白。受保护的制剂包含10mM至60mM的选自谷胱甘肽（GSH），烟酰胺腺嘌呤二核苷酸（NADH），尼古丁腺嘌呤二核苷酸磷酸（NADPH）的化合物及其在血红蛋白 - 含有液体。通过加入10mM至60mM的选自谷胱甘肽（GSH），烟酰胺腺嘌呤二核苷酸（NADH），尼古丁腺嘌呤二核苷酸磷酸（NADPH）和它们的混合物的化合物组合，可以恢复制剂中的氧化血红蛋白其中血红蛋白全部或部分转化为高铁血红蛋白，并将流体存储在高于流体凝固点的血液制品的储存温度上足以将高铁血红蛋白转化成血红蛋白的时间。

4. 发明授权

US6033708A Method for producing sterile filterable liposome dispersion 失效
标题翻译：生产无菌可过滤脂质体分散体的方法
公开(公告)号：US6033708A
公开(公告)日：2000-03-07
申请号：US940739
申请日：1997-09-30
申请人： Victoria Kwasiborski , Richard O. Cliff , Alan S. Rudolph
发明人： Victoria Kwasiborski , Richard O. Cliff , Alan S. Rudolph
IPC分类号： A61K9/127 , A61K9/133 , A61K38/42
CPC分类号： A61K9/1277 , A61K38/42 , A61K9/127
摘要： The present invention relates to a composition and method of producing noggregating, filterable dispersion of liposome encapsulated agent or therapeutic of interest, specifically hemoglobin. A key step in the method of the present invention is the addition of a passivating protein prior to hydration of the liposomes with the agent of interest. The resulting dispersion is composed of unilamellar vesicles having a diameter of less than or equal to 0.2 microns.
摘要翻译：本发明涉及生产脂质体包封剂或感兴趣的治疗剂，特别是血红蛋白的非聚集，可过滤的分散体的组合物和方法。本发明方法的关键步骤是在脂质体与感兴趣的试剂水合之前加入钝化蛋白质。所得分散体由直径小于或等于0.2微米的单层囊泡组成。

5. 发明授权

US5690963A Freeze dried red blood cells 失效
标题翻译：冷冻干红细胞
公开(公告)号：US5690963A
公开(公告)日：1997-11-25
申请号：US497708
申请日：1995-06-30
申请人： Barry J. Spargo , Alan S. Rudolph , Byeong S. Chang , Thomas R. Groel, II
发明人： Barry J. Spargo , Alan S. Rudolph , Byeong S. Chang , Thomas R. Groel, II
IPC分类号： A01N1/02 , A61K35/18
CPC分类号： A61K35/18 , A01N1/02 , A01N1/0226 , A01N1/0289
摘要： A process and medium are disclosed for the lyophilization of cells, specifically red blood cells and platelets, and cell-like matter, which comprises the use of solution including a carbohydrate, and biocompatible polymers to permit reconstitution of transfusably useful cells which are viable by the measure of ATP and 2,3 DPG.
摘要翻译：公开了一种用于冻干细胞，特别是红细胞和血小板以及细胞样物质的方法和培养基，其包括使用包含碳水化合物的溶液和生物相容性聚合物，以允许重构可转移有用的细胞，其可由测量ATP和2,3 DPG。

6. 发明申请

US20130274437A1 STABILIZED AMYLOID-BETA OLIGOMERS AND USES THEREOF 有权
标题翻译：稳定的AMYLOID-BETA低聚物及其用途
公开(公告)号：US20130274437A1
公开(公告)日：2013-10-17
申请号：US13698857
申请日：2011-05-24
申请人： Roxanne Duan , Jonathan Moll , Alan S. Rudolph
发明人： Roxanne Duan , Jonathan Moll , Alan S. Rudolph
IPC分类号： C07K14/47 , C07K1/14
CPC分类号： C07K14/4711 , C07K1/14
摘要： The present invention pertains to stabilized Aβ oligomer compositions. Methods for generating stabilized Aβ oligomer compounds are also provided herein. Additionally, screening assays employing the Aβ oligomer compounds and methods for generating therapeutics with the Aβ oligomers are also provided. In a particular embodiment, the Aβ oligomer described herein is comprised of Aβ42 peptide.
摘要翻译：本发明涉及稳定的Abeta低聚物组合物。本文还提供了产生稳定的Aβ低聚物化合物的方法。另外，还提供了使用Abeta寡聚物化合物的筛选测定法和用Aβ产生治疗剂的方法。在一个具体实施方案中，本文所述的Aβ寡聚体由Abeta42肽组成。

7. 发明授权

US07811558B2 Use of stabilized platelets as hemostatic agent 有权
标题翻译：使用稳定的血小板作为止血剂
公开(公告)号：US07811558B2
公开(公告)日：2010-10-12
申请号：US11202376
申请日：2005-08-12
申请人： David Ho , Cindy S. Orser , Alan S. Rudolph
发明人： David Ho , Cindy S. Orser , Alan S. Rudolph
IPC分类号： A01N65/00
CPC分类号： A01N1/00 , A61K35/19 , G01N33/86
摘要： The present invention provides compositions comprising freeze-dried platelets, microparticles, or both for use as a hemostat, such as for treating bleeding or injuries associated with bleeding. It also provides methods of treating injuries or wounds, and methods of causing blood to clot. Likewise, it provides methods of promoting healing of wounds or of healing wounds.
摘要翻译：本发明提供了包含冷冻干燥的血小板，微粒或两者用作止血剂的组合物，例如用于治疗与出血相关的出血或损伤。它还提供了治疗损伤或伤口的方法，以及引起血液凝结的方法。同样，它提供了促进伤口愈合或愈合伤口的方法。

8. 发明授权

US06436705B1 Shape stabilized erythrocytes 失效
标题翻译：形状稳定的红细胞
公开(公告)号：US06436705B1
公开(公告)日：2002-08-20
申请号：US10046297
申请日：2002-01-16
申请人： Irina B. Bakaltcheva , Alan S. Rudolph , Barry J. Spargo , Samuel B. Leslie , Thomas R. Groel
发明人： Irina B. Bakaltcheva , Alan S. Rudolph , Barry J. Spargo , Samuel B. Leslie , Thomas R. Groel
IPC分类号： C12N508
CPC分类号： A01N1/0221 , A01N1/02
摘要： One aspect of the present invention is a method for storing tissues and cells (typically erythrocytes) having the step of (1) stabilizing the cells with a reversible stabilizing agent. This method typically will have the additional steps of (2) loading the cells with a cryoprotectant, and typically (3) storing the cells in liquid, frozen, or dry state. This method will also typically have the additional step of (4) prior to use, reversing the stabilization reaction. Preferably, the erythrocytes are pre-treated with CO to complex the hemoglobin with CO. It is anticipated that a practical method according to the invention will include reoxygenation of the eryathrocytes, and also washing out reagents prior to in vivo use. Another aspect of the present invention is an erythrocyte that has had its shape stabilized by the reversible crosslinking of proteins in the erythrocyte, such as the structural proteins of the cytoskeleton. Another aspect of the invention is a population of such reversibly cross-linked erythrocytes. Another aspect of the invention is the in vivo use of such erythrocytes, after the reversal of the crosslinking reaction. The use of more gentle, reversible cross-linking as described below is desirable to result in the recovery of erythrocyte deformability and extended post-transfusion survival.
摘要翻译：本发明的一个方面是用于存储具有以下步骤的组织和细胞（通常为红细胞）的方法：（1）用可逆稳定剂稳定细胞。该方法通常将具有（2）用冷冻保护剂加载细胞的另外的步骤，并且通常（3）将细胞储存在液体，冷冻或干燥状态。该方法通常也将在使用前具有（4）的附加步骤，从而逆转稳定化反应。优选地，用CO预处理红细胞以使CO与血红蛋白复合。根据本发明的实际方法预期将包括红细胞的再氧化，以及在体内使用之前洗去试剂。本发明的另一方面是通过红细胞中蛋白质的可逆交联（例如细胞骨架的结构蛋白质）使其形状稳定的红细胞。本发明的另一方面是这种可逆交联的红细胞的群体。本发明的另一方面是在反应交联反应后体内使用这种红细胞。使用如下所述的更温和的可逆交联是期望的，以导致红细胞变形能力的恢复和输血后的生存期延长。

9. 发明授权

US06358678B1 Applications of reversible crosslinking and co-treatment in stabilization and viral inactivations of erythrocytes 失效
标题翻译：可逆交联和共处理在红细胞稳定化和病毒灭活中的应用
公开(公告)号：US06358678B1
公开(公告)日：2002-03-19
申请号：US09022341
申请日：1998-02-11
申请人： Irina B. Bakaltcheva , Alan S. Rudolph , Barry J. Spargo , Samuel B. Leslie , Thomas R. Groel
发明人： Irina B. Bakaltcheva , Alan S. Rudolph , Barry J. Spargo , Samuel B. Leslie , Thomas R. Groel
IPC分类号： A01N102
CPC分类号： A01N1/0221 , A01N1/02
摘要： One aspect of the present invention is a method for storing tissues and cells (typically erythrocytes) having the step of (1) stabilizing the cells with a reversible stabilizing agent. This method typically will have the additional steps of (2) loading the cells with a cryoprotectant, and typically (3) storing the cells in liquid, frozen, or dry state. This method will also typically have the additional step of (4) prior to use, reversing the stabilization reaction. Preferably, the erythrocytes are pre-treated with CO to complex the hemoglobin with CO. It is anticipated that a practical method according to the invention will include reoxygenation of the erythrocytes, and also washing out reagents prior to in vivo use. Another aspect of the present invention is an erythrocyte that has had its shape stabilized by the reversible crosslinking of proteins in the erythrocyte, such as the structural proteins of the cytoskeleton. Another aspect of the invention is a population of such reversibly crosslinked erythrocytes. Another aspect of the invention is the in vivo use of such erythrocytes, after the reversal of the crosslinking reaction. The use of more gentle, reversible cross-linking as described below is desirable to result in the recovery of erythrocyte deformability and extended post-transfusion survival.
摘要翻译：本发明的一个方面是用于存储具有以下步骤的组织和细胞（通常为红细胞）的方法：（1）用可逆稳定剂稳定细胞。该方法通常将具有（2）用冷冻保护剂加载细胞的另外的步骤，并且通常（3）将细胞储存在液体，冷冻或干燥状态。该方法通常也将在使用前具有（4）的附加步骤，从而逆转稳定化反应。优选地，红细胞用CO预处理以使CO与血红蛋白复合。根据本发明的实际方法可以预期，包括红细胞的再氧化，以及在体内使用前洗涤试剂。本发明的另一方面是通过红细胞中蛋白质的可逆交联（例如细胞骨架的结构蛋白质）使其形状稳定的红细胞。本发明的另一方面是这种可逆交联的红细胞的群体。本发明的另一方面是在反应交联反应后体内使用这种红细胞。使用如下所述的更温和的可逆交联是期望的，以导致红细胞变形能力的恢复和输血后的生存期延长。

10. 发明授权

US5705191A Sustained delivery of active compounds from tubules, with rational control 失效
标题翻译：持续输送活性化合物从小管，合理控制
公开(公告)号：US5705191A
公开(公告)日：1998-01-06
申请号：US523725
申请日：1995-08-18
申请人： Ronald R. Price , Joel M. Schnur , Alan S. Rudolph , Jonathan Selinger , Alok Singh , Bruce P. Gaber
发明人： Ronald R. Price , Joel M. Schnur , Alan S. Rudolph , Jonathan Selinger , Alok Singh , Bruce P. Gaber
IPC分类号： A61K9/00 , A61K9/127 , A61K9/24
CPC分类号： A61K9/0092 , A61K9/1274
摘要： The present invention is a method for releasing an active agent into a use nvironment, by disposing this active agent within the lumen of a population of tubules, and disposing the tubules into a use environment, either directly or in some matrix such as a paint in contact with the use environment. The tubules have a preselected release profile to provide a preselected release rate curve. The preselected release profile may be achieved by controlling the length or length distribution of the tubules, or by placing degradable endcaps over some or all of the tubules in the population, or by combinations of these methods. The invention is also a population of tubules having a preselected release profile to provide a preselected release rate curve.
摘要翻译：本发明是一种将活性剂释放到使用环境中的方法，其通过将该活性剂置于一群小管的内腔内，并将该小管直接或以某种基质如涂料与使用环境接触。小管具有预先选择的释放曲线以提供预选的释放速率曲线。预选释放曲线可以通过控制小管的长度或长度分布，或者通过将可降解端帽置于群体中的一些或全部小管上，或通过这些方法的组合来实现。本发明还是具有预选释放曲线以提供预选释放速率曲线的小管群。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式