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1. 发明申请

US20140051134A1 METHOD FOR PRODUCING PHOSPHITE DEHYDROGENASE PROTEIN AND USE THEREOF 有权
标题翻译：生产磷酸脱氢蛋白的方法及其用途
公开(公告)号：US20140051134A1
公开(公告)日：2014-02-20
申请号：US14113346
申请日：2012-04-16
申请人： Akio Kuroda , Ryuichi Hirota
发明人： Akio Kuroda , Ryuichi Hirota
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12N9/0004 , C12P17/182 , C12Y120/01001
摘要： In order to provide: a phosphite dehydrogenase protein having both improved solubility and improved heat stability; a method for producing a gene encoding the phosphite dehydrogenase protein; a method for producing the phosphite dehydrogenase protein; and use of the phosphite dehydrogenase protein, (i) a phosphite dehydrogenase protein having a specific amino acid sequence and (ii) a gene encoding the phosphite dehydrogenase protein are used.
摘要翻译：为了提供：具有改善的溶解度和改善的热稳定性的亚磷酸酯脱氢酶蛋白质; 生产编码亚磷酸脱氢酶蛋白质的基因的方法; 一种生产亚磷酸脱氢酶蛋白的方法; 和使用亚磷酸酯脱氢酶蛋白质，（i）具有特定氨基酸序列的亚磷酸脱氢酶蛋白质和（ii）编码亚磷酸酯脱氢酶蛋白质的基因。

2. 发明授权

US09273290B2 Method for producing phosphite dehydrogenase protein and use thereof 有权
标题翻译：亚磷酸脱氢酶蛋白的生产方法及其应用
公开(公告)号：US09273290B2
公开(公告)日：2016-03-01
申请号：US14113346
申请日：2012-04-16
申请人： Akio Kuroda , Ryuichi Hirota
发明人： Akio Kuroda , Ryuichi Hirota
IPC分类号： C12N9/04 , C12P17/18 , C12N9/02
CPC分类号： C12N9/0006 , C12N9/0004 , C12P17/182 , C12Y120/01001
摘要： In order to provide: a phosphite dehydrogenase protein having both improved solubility and improved heat stability; a method for producing a gene encoding the phosphite dehydrogenase protein; a method for producing the phosphite dehydrogenase protein; and use of the phosphite dehydrogenase protein, (i) a phosphite dehydrogenase protein having a specific amino acid sequence and (ii) a gene encoding the phosphite dehydrogenase protein are used.
摘要翻译：为了提供：具有改善的溶解度和改善的热稳定性的亚磷酸酯脱氢酶蛋白质; 生产编码亚磷酸脱氢酶蛋白质的基因的方法; 一种生产亚磷酸脱氢酶蛋白的方法; 和使用亚磷酸酯脱氢酶蛋白质，（i）具有特定氨基酸序列的亚磷酸脱氢酶蛋白质和（ii）编码亚磷酸酯脱氢酶蛋白质的基因。

3. 发明授权

US08394602B2 Luminescent method for measuring endotoxin 有权
标题翻译：用于测量内毒素的发光方法
公开(公告)号：US08394602B2
公开(公告)日：2013-03-12
申请号：US12734485
申请日：2008-11-11
申请人： Akio Kuroda , Kenichi Noda
发明人： Akio Kuroda , Kenichi Noda
IPC分类号： C12Q1/56
CPC分类号： G01N21/763 , C12Q1/66 , G01N33/56911 , G01N33/579
摘要： The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device.
摘要翻译：本发明提供了一种方法，其包括使样品，可通过与内毒素结合而活化的因子C的试剂与包含与肽结合的发光底物的合成发光底物反应，从而将发光底物从所述合成发光基板允许发光酶作用于在所述发光底物释放步骤中释放的所述发光基板，用于测量所述发光强度，并且基于在所述发光测量步骤中获得的测量值来定量所述样品中的内毒素水平，在不使用任何专用测量装置的情况下，能够以常规的内毒素测量方法无法检测的水平简单且快速地测量内毒素的方法。

4. 发明授权

US08133542B2 Resin-coated metal plate and drawn cans using these plates 有权
标题翻译：树脂涂覆的金属板和使用这些板的拉伸罐
公开(公告)号：US08133542B2
公开(公告)日：2012-03-13
申请号：US12847610
申请日：2010-07-30
申请人： Akio Kuroda , Takashi Iwai , Kazuhiro Sato
发明人： Akio Kuroda , Takashi Iwai , Kazuhiro Sato
IPC分类号： B05D3/02
CPC分类号： B32B15/08 , B32B1/02 , B32B7/02 , B32B15/09 , B32B15/18 , B32B15/20 , B32B27/08 , B32B27/28 , B32B27/308 , B32B27/36 , B32B2255/06 , B32B2255/26 , B32B2307/554 , B32B2307/704 , B32B2307/714 , B32B2439/66 , B32B2439/70 , B65D1/28 , Y10T428/1355 , Y10T428/31681 , Y10T428/31786
摘要： The present invention offers resin-coated metal plates and methods of making, in which the resin-coated metal plates prevent wicket scars from occurring during processes of varnish, print and dry, and there are few scars on the resin-coated surface, the inner surface side of the can and that have superior corrosion resistance and workability, and drawn cans using these metal plates. Therefore, resin film that is applied to the metal plates, is comprised of two layers of a crystallized saturated polyester resin layer [A] and a layer composed of resin [B] that is comprised of saturated polyester resin (i) and ionomer resin (ii), wherein the layer composed of resin [B] is laminated on the above-mentioned metal plate to tightly contact with it, and on the polar surface of the saturated polyester resin layer that is crystallized [A] a highly crystallized layer (X) is formed. A drawn can is formed using the resin-coated metal plate.
摘要翻译：本发明提供了树脂涂覆的金属板和制造方法，其中树脂涂覆的金属板防止在清漆，印刷和干燥过程中出现小网痕迹，并且在树脂涂覆表面上几乎没有疤痕罐的表面侧，具有优异的耐腐蚀性和可加工性，以及使用这些金属板的拉伸罐。因此，施加到金属板上的树脂膜由两层结晶的饱和聚酯树脂层[A]和由饱和聚酯树脂（i）和离聚物树脂（i）组成的树脂[B]组成的层组成， ii），其中由树脂[B]组成的层层叠在上述金属板上以紧密接触，并且在结晶的饱和聚酯树脂层的极性表面上[A]高度结晶的层（X ）形成了。使用树脂涂覆的金属板形成拉伸罐。

5. 发明申请

US20100311093A1 METHOD OF AMPLIFYING ATP AND USER THEREOF 有权
标题翻译：放大ATP及其用途的方法
公开(公告)号：US20100311093A1
公开(公告)日：2010-12-09
申请号：US12769100
申请日：2010-04-28
申请人： Akio Kuroda
发明人： Akio Kuroda
IPC分类号： C12Q1/48 , C12N9/12
CPC分类号： C12N9/1229 , C12N15/62 , C12P19/32 , C12Q1/485
摘要： The ATP amplification method is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. Also provided is an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
摘要翻译： ATP扩增方法是通过使多磷酸激酶和腺苷酸激酶（不含有ADP的融合蛋白）的融合蛋白（PPK-ADK）作用于不含有ADP的混合物来扩增和检测非常微量的外源ATP的方法 ATP，AMP和多磷酸盐化合物。还提供了可以扩增和检测单细胞水平的ATP的超灵敏ATP扩增方法，以及基于该ATP扩增方法的超敏感微生物测定法。

6. 发明申请

US20080213754A1 Method of Amplifying Atp and Use Thereof 有权
标题翻译：放大和使用它的方法
公开(公告)号：US20080213754A1
公开(公告)日：2008-09-04
申请号：US10566957
申请日：2004-07-27
申请人： Akio Kuroda
发明人： Akio Kuroda
IPC分类号： C12Q1/68 , C12P19/30 , C12N9/12
CPC分类号： C12N9/1229 , C12N15/62 , C12P19/32 , C12Q1/485
摘要： The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
摘要翻译：本发明的ATP扩增方法是通过使多磷酸激酶的融合蛋白（PPK-ADK）和不含ADP的融合蛋白的腺苷酸激酶（PPK-ADK）作用而扩增和检测非常微量的外源ATP的方法在ATP，AMP和多磷酸盐化合物的混合物上。本发明还提供了可以扩增和检测单细胞水平的ATP的超灵敏ATP扩增方法，以及基于该ATP扩增方法的超敏感微生物测定法。

7. 发明授权

US5951903A Method and apparatus for joining metal pieces 失效
标题翻译：用于连接金属片的方法和装置
公开(公告)号：US5951903A
公开(公告)日：1999-09-14
申请号：US513789
申请日：1995-12-29
申请人： Shigeru Isoyama , Takeshi Hirabayashi , Hideyuki Nikaido , Toshiaki Amagasa , Nozomu Tamura , Takahiro Yamasaki , Masashi Osada , Hirosuke Yamada , Toshisada Takechi , Norio Takashima , Junzo Nitta , Shuji Amanuma , Kanji Hayashi , Akio Kuroda , Yoshiki Mito , Kunio Miyamoto , Kazuo Morimoto , Ikuo Wakamoto , Kazuya Tsurusaki , Hideo Sakamoto , Michio Hashimoto , Hiroyuki Nakano , Toshinobu Eguchi , Fumihiro Maeda
发明人： Shigeru Isoyama , Takeshi Hirabayashi , Hideyuki Nikaido , Toshiaki Amagasa , Nozomu Tamura , Takahiro Yamasaki , Masashi Osada , Hirosuke Yamada , Toshisada Takechi , Norio Takashima , Junzo Nitta , Shuji Amanuma , Kanji Hayashi , Akio Kuroda , Yoshiki Mito , Kunio Miyamoto , Kazuo Morimoto , Ikuo Wakamoto , Kazuya Tsurusaki , Hideo Sakamoto , Michio Hashimoto , Hiroyuki Nakano , Toshinobu Eguchi , Fumihiro Maeda
IPC分类号： B21B1/26 , B21B15/00 , B23K13/01 , B23K13/02 , H05B6/10 , B21B45/00
CPC分类号： B21B15/0085 , B23K13/015 , B23K13/02 , B21B1/26 , B23K2201/18
摘要： According to the invention, the rear end of a preceding metal piece and a front end of a succeeding metal piece are heated, pressed and joined prior to the finishing hot rolling. An alternating magnetic field running through the metal pieces in the thickness direction thereof is generated in an end region on opposed faces of the respective metal pieces to perform heating. Another alternating magnetic field whose direction is reverse with respect to that of the former alternating magnetic field is partially generated in the end region on the opposed faces of the metal pieces and in either a region where the metal pieces exist or a region outside width ends of the metal pieces. This assures a uniform heating of the end region on the opposed faces of the metal pieces over their full width to reliably join the both metal pieces to each other.
摘要翻译： PCT No.PCT / JP94 / 02113 Sec。 371 1995年12月29日第 102（e）1995年12月29日PCT PCT 1994年12月15日PCT公布。公开号WO95 / 16525 日期：1995年6月22日根据本发明，在精加工热轧之前，先前金属片的后端和后面的金属片的前端被加热，压制和接合。在各个金属片的相对面上的端部区域中产生沿其厚度方向穿过金属片的交变磁场，以进行加热。在金属片的相对面上的端部区域和金属片存在的区域或外侧宽度端部的区域中部分地产生相对于前者的交变磁场的方向相反的另一个交变磁场金属件。这确保了金属片的相对面上的端部区域在其全宽度上的均匀加热，以将两个金属片彼此可靠地接合。

8. 发明授权

US5488330A Bias circuit for controlling bias voltage of differential amplifier 失效
标题翻译：用于控制差分放大器偏置电压的偏置电路
公开(公告)号：US5488330A
公开(公告)日：1996-01-30
申请号：US361583
申请日：1994-12-22
申请人： Hideaki Masuoka , Akio Kuroda
发明人： Hideaki Masuoka , Akio Kuroda
IPC分类号： H03G11/00 , H03D7/14 , H03F1/32 , H03F3/45 , H04B1/26
CPC分类号： H03F3/4521 , H03F1/3211 , H03F3/45085 , H03F3/45089 , H03F2203/45366 , H03F2203/45542 , H03F2203/45544 , H03F2203/45574 , H03F2203/45702
摘要： A first differential amplifier includes first and second transistors. A bias circuit includes a second differential amplifier, a constant-current source, and a capacitor. The bases of third and fourth transistors constituting a second differential amplifier, are connected to those of the first and second transistors, respectively. The constant-current source supplies a constant current to the third and fourth transistors, and one end of the capacitor is connected to a connection node of the constant-current source and fourth transistor. In the bias circuit, when a high-level signal is supplied to the base of the first transistor, the capacitor is charged and discharged in accordance with the operations of the third and fourth transistors, with the result that the base potential of the first and second transistors is stabilized, and the operation current flowing through the first differential amplifier is prevented from increasing.
摘要翻译：第一差分放大器包括第一和第二晶体管。偏置电路包括第二差分放大器，恒流源和电容器。构成第二差分放大器的第三和第四晶体管的基极分别连接到第一和第二晶体管的基极。恒流源向第三和第四晶体管提供恒定电流，并且电容器的一端连接到恒流源和第四晶体管的连接节点。在偏置电路中，当向第一晶体管的基极提供高电平信号时，根据第三和第四晶体管的操作对电容器进行充电和放电，结果是第一和第二晶体管的基极电位第二晶体管稳定，并且防止流过第一差分放大器的工作电流增加。

9. 发明授权

US4631717A High density information records of an electrostatic capacitance type 失效
标题翻译：高密度信息记录的静电电容型
公开(公告)号：US4631717A
公开(公告)日：1986-12-23
申请号：US675014
申请日：1984-11-26
申请人： Kazuhira Namikawa , Toshiaki Hamaguchi , Mutsuaki Nakamura , Akio Kuroda , Akio Hata , Noriki Fujii
发明人： Kazuhira Namikawa , Toshiaki Hamaguchi , Mutsuaki Nakamura , Akio Kuroda , Akio Hata , Noriki Fujii
IPC分类号： C08K3/02 , C08K3/04 , C08K5/101 , C08K5/103 , C08L27/06 , C08L51/06 , G11B9/06 , G11B5/00
CPC分类号： G11B9/068 , C08K5/101 , C08K5/103 , Y10T428/31678 , Y10T428/31786
摘要： High density information records formed from conductive resin compositions which comprise a graft polymer obtained by subjecting vinyl chloride monomer to graft polymerization with a copolymer of ethylene and vinyl acetate having a content of vinyl acetate of not less than 35 wt % and a melt index up to 20 and prepared according to a solution polymerization technique. The conductive composition further comprises predetermined amounts of a conductive material and a lubricant of a specific type. The lubricant is an ester between aliphatic acid and alcohol having a hydroxyl value not larger than 15.
摘要翻译：由导电性树脂组合物形成的高密度信息记录，其包含通过使氯乙烯单体与乙酸乙烯酯的含量不小于35重量％的乙烯和乙酸乙烯酯的共聚物进行接枝聚合而得到的接枝聚合物，熔体指数高达 20并根据溶液聚合技术制备。导电组合物还包含预定量的导电材料和特定类型的润滑剂。润滑剂是脂肪酸与羟基值不大于15的醇之间的酯。

10. 发明授权

US4547545A High density information record made of a conductive resin composition comprising a specific type of dimethylpolysiloxane 失效
标题翻译：由包含特定类型的二甲基聚硅氧烷的导电树脂组合物制成的高密度信息记录
公开(公告)号：US4547545A
公开(公告)日：1985-10-15
申请号：US603761
申请日：1984-04-13
申请人： Toshiaki Hamaguchi , Kazuhira Namikawa , Toshikazu Goshima , Mutsuaki Nakamura , Akio Kuroda , Yoshiaki Kudo
发明人： Toshiaki Hamaguchi , Kazuhira Namikawa , Toshikazu Goshima , Mutsuaki Nakamura , Akio Kuroda , Yoshiaki Kudo
IPC分类号： C08K3/04 , G11B9/06 , H01B1/24 , C08K5/54
CPC分类号： C08K3/04 , G11B9/068 , H01B1/24
摘要： A high density information record of the electrostatic capacitance type which comprises a record substrate on which signal information is recorded as geometric variations. The substrate is made of a conductive resin composition comprising a thermoplastic resin, a conductive material, and a defined amount of a specific type of dimethylpolysiloxane lubricant having a degree of inhomogeneity of 1.0 to 1.3 and a degree of polymerization of 12 to 60.
摘要翻译：静电电容型的高密度信息记录包括记录信号作为几何变化的记录基板。该基片由包含热塑性树脂，导电材料和一定量的不均匀度为1.0至1.3且聚合度为12至60的特定类型二甲基聚硅氧烷润滑剂的导电树脂组合物制成。

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