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1. 发明申请

US20050112715A1 Methods for the identification of inhibitors of pectin esterase expression or activity in plants 审中-公开
标题翻译：用于鉴定植物中果胶酯酶表达或活性抑制剂的方法
公开(公告)号：US20050112715A1
公开(公告)日：2005-05-26
申请号：US10717879
申请日：2003-11-20
申请人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
发明人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
IPC分类号： C12Q1/44 , A01N25/00
CPC分类号： C12Q1/44 , G01N2500/00
摘要： The present inventors have discovered that pectin esterase is essential for plant growth. Specifically, the inhibition of pectin esterase gene expression resulted in seedlings that were damaged, short, and stunted at a very early growth stage. Thus, pectin esterase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit pectin esterase expression or activity, comprising: contacting a compound with a pectin esterase and detecting the presence and/or absence of binding between said compound and said pectin esterase, or detecting a decrease in pectin esterase expression or activity. The methods of the invention are useful for the identification of herbicides.
摘要翻译：本发明人发现，果胶酯酶对植物生长至关重要。具体来说，果胶酯酶基因表达的抑制导致幼苗在非常早期的生长阶段受损，短缺和发育迟缓。因此，果胶酯酶可以用作鉴定除草剂的靶标。因此，本发明提供了鉴定抑制果胶酯酶表达或活性的化合物的方法，包括：使化合物与果胶酯酶接触并检测所述化合物与所述果胶酯酶之间的结合的存在和/或不存在，或检测果胶酯酶表达或活性降低。本发明的方法可用于鉴定除草剂。

2. 发明授权

US06770452B1 Methods for the identification of inhibitors of serine acetyltransferase activity in plants 失效
标题翻译：鉴定植物中丝氨酸乙酰转移酶活性抑制剂的方法
公开(公告)号：US06770452B1
公开(公告)日：2004-08-03
申请号：US10402818
申请日：2003-03-28
申请人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
发明人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
IPC分类号： C12Q148
CPC分类号： C12N9/1029 , C12N15/8261 , C12N15/8274 , C12Q1/48 , G01N2333/91057 , G01N2500/02 , Y02A40/146
摘要： The present inventors have discovered that serine acetyltransferase (SAT) is essential for plant growth. Specifically, the inhibition of SAT gene expression in plant seedlings results in reduced growth and altered pigmentation. Thus, SAT is useful as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of herbicides by measuring the activity of an SAT in the presence and absence of a compound, wherein an alteration of SAT activity in the presence of the compound indicates the compound as a candidate for a herbicide.
摘要翻译：本发明人发现丝氨酸乙酰转移酶（SAT）对于植物生长是必需的。具体地说，在植物幼苗中抑制SAT基因表达导致生长减少和色素沉着改变。因此，SAT可用作鉴定除草剂的靶标。因此，本发明提供了通过在化合物存在和不存在下测量SAT的活性来鉴定除草剂的方法，其中在化合物存在下SAT活性的改变表示作为除草剂候选物的化合物。

3. 发明申请

US20050130118A1 Methods for identifying inhibitors of chlorophyll synthase 审中-公开
标题翻译：鉴定叶绿素合酶抑制剂的方法
公开(公告)号：US20050130118A1
公开(公告)日：2005-06-16
申请号：US10737014
申请日：2003-12-16
申请人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
发明人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
IPC分类号： C12Q1/00 , G01N33/573
CPC分类号： G01N33/573
摘要： The present inventors have discovered that antisense suppression of a chlorophyll synthase (CS) gene results in plants exhibiting one or more of chlorosis, reduced growth, and altered development. Thus, the present inventors have discovered that the protein encoded by the CS gene is essential for normal plant growth and development, and is useful as a target for the identification of compounds as antibiotics and herbicides, especially herbicides. The present invention is directed to methods for identifying inhibitors of a CS enzyme by incubating a CS polypeptide with a chlorophyllide and a phopholipid substrate in the presence and absence of a test compound under conditions suitable for the CS enzyme activity, adding a solution to the incubation reactions comprising a water immiscible organic solvent, a water-soluble alcohol, and a water-soluble dye that absorbs in the range of one or both the excitation and emission wavelength ranges of the chlorophyllide substrate, and measuring the fluorescence of the incubation reactions at from about 650 to 750 nm, using from about 425 to 445 nm as excitation wavelength, wherein a decrease in the fluorescence in the presence of the test compound indicates that the compound is a CS inhibitor.
摘要翻译：本发明人已经发现，叶绿素合酶（CS）基因的反义抑制导致植物表现出一种或多种褪绿，生长减少和改变发育。因此，本发明人已经发现，由CS基因编码的蛋白质对于正常的植物生长和发育是必需的，并且可用作鉴定作为抗生素和除草剂，特别是除草剂的化合物的靶标。本发明涉及通过在适合于CS酶活性的条件下，在存在和不存在测试化合物的情况下，将CS多肽与叶绿素和磷脂底物一起温育来鉴定CS酶抑制剂的方法，向培养基中加入溶液包括与水不溶混的有机溶剂，水溶性醇和在所述叶绿素底物的激发和发射波长范围中的一个或两个的范围内吸收的水溶性染料的反应，以及测量从约650至750nm，使用约425至445nm作为激发波长，其中在测试化合物存在下荧光的降低表明该化合物是CS抑制剂。

4. 发明授权

US06353155B1 Method for transforming plants 有权
标题翻译：转化植物的方法
公开(公告)号：US06353155B1
公开(公告)日：2002-03-05
申请号：US09607306
申请日：2000-06-30
申请人： Andreas S. Klöti , Rao Mulpuri
发明人： Andreas S. Klöti , Rao Mulpuri
IPC分类号： A01H100
CPC分类号： C12N15/8205
摘要： A method for preparing transgenic plants and seeds is claimed. The method is particularly useful for high-throughput transformation of plants, such as Arabidopsis thaliana, using many different types of DNA sequences of interest.
摘要翻译：要求保护转基因植物和种子的方法。该方法对于使用许多不同类型的感兴趣的DNA序列的拟南芥植物的高通量转化特别有用。

5. 发明授权

US08665512B2 Multi-pane electrochromic windows 有权
标题翻译：多窗格电致变色窗
公开(公告)号：US08665512B2
公开(公告)日：2014-03-04
申请号：US13619772
申请日：2012-09-14
申请人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
发明人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
IPC分类号： G02F1/153
CPC分类号： G02F1/161 , C03C17/34 , C03C17/36 , C03C17/3657 , C03C17/3681 , E06B7/28 , E06B9/24 , E06B2009/2464 , E06B2009/2643 , G02F1/1347 , G02F1/1523 , G02F1/153 , G02F1/155 , G02F1/157 , G02F2001/1555 , H05B3/86 , H05B2203/013
摘要： Window units, for example insulating glass units (IGU's), that have at least two panes, each pane having an electrochromic device thereon, are described. Two optical state devices on each pane of a dual-pane window unit provide window units having four optical states. Window units described allow the end user a greater choice of how much light is transmitted through the electrochromic window. Also, by using two or more window panes, each with its own electrochromic device, registered in a window unit, visual defects in any of the individual devices are negated by virtue of the extremely small likelihood that any of the visual defects will align perfectly and thus be observable to the user.
摘要翻译：描述具有至少两个窗格的窗单元，例如中空玻璃单元（IGU），每个窗格上具有电致变色设备。双窗格窗单元的每个窗格上的两个光学状态设备提供具有四个光学状态的窗口单元。描述的窗口单元允许最终用户更多地选择通过电致变色窗口传输多少光。此外，通过使用两个或更多个窗玻璃，每个具有其自己的电致变色装置，登记在窗口单元中，任何单个装置中的视觉缺陷由于任何视觉缺陷将完美对准而非常小的可能性而被否定，并且因此可以观察到用户。

6. 发明申请

US20120033287A1 MULTI-PANE ELECTROCHROMIC WINDOWS 有权
标题翻译：多平板电子窗
公开(公告)号：US20120033287A1
公开(公告)日：2012-02-09
申请号：US12851514
申请日：2010-08-05
申请人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
发明人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
IPC分类号： G02F1/161 , B32B17/00
CPC分类号： G02F1/161 , C03C17/34 , C03C17/36 , C03C17/3657 , C03C17/3681 , E06B7/28 , E06B9/24 , E06B2009/2464 , E06B2009/2643 , G02F1/1347 , G02F1/1523 , G02F1/153 , G02F1/155 , G02F1/157 , G02F2001/1555 , H05B3/86 , H05B2203/013
摘要： Window units, for example insulating glass units (IGU's), that have at least two panes, each pane having an electrochromic device thereon, are described. Two optical state devices on each pane of a dual-pane window unit provide window units having four optical states. Window units described allow the end user a greater choice of how much light is transmitted through the electrochromic window. Also, by using two or more window panes, each with its own electrochromic device, registered in a window unit, visual defects in any of the individual devices are negated by virtue of the extremely small likelihood that any of the visual defects will align perfectly and thus be observable to the user.
摘要翻译：描述具有至少两个窗格的窗单元，例如中空玻璃单元（IGU），每个窗格上具有电致变色设备。双窗格窗单元的每个窗格上的两个光学状态设备提供具有四个光学状态的窗口单元。描述的窗口单元允许最终用户更多地选择通过电致变色窗口传输多少光。此外，通过使用两个或更多个窗玻璃，每个具有其自己的电致变色装置，登记在窗口单元中，任何单个装置中的视觉缺陷由于任何视觉缺陷将完美对准而非常小的可能性而被否定，并且因此可以观察到用户。

7. 发明授权

US5763008A Chemical vapor deposition of mullite coatings 失效
标题翻译：莫来石涂层的化学气相沉积
公开(公告)号：US5763008A
公开(公告)日：1998-06-09
申请号：US368814
申请日：1995-01-06
申请人： Vinod Sarin , Rao Mulpuri
发明人： Vinod Sarin , Rao Mulpuri
IPC分类号： C04B35/185 , C04B35/622 , C04B35/626 , C04B41/45 , C04B41/81 , C23C16/40 , C23C16/455 , C23C16/00
CPC分类号： C04B41/81 , C04B35/185 , C04B35/62222 , C04B35/626 , C04B41/4531 , C23C16/401 , C23C16/455
摘要： This invention is directed to the creation of crystalline mullite coatings having uniform microstructure by chemical vapor deposition (CVD). The process comprises the steps of establishing a flow of reactants which will yield mullite in a CVD reactor, and depositing a crystalline coating from the reactant flow. The process will yield crystalline coatings which are dense and of uniform thickness.
摘要翻译：本发明涉及通过化学气相沉积（CVD）产生具有均匀微观结构的结晶莫来石涂层。该方法包括以下步骤：建立在CVD反应器中产生莫来石的反应物流，以及从反应物流中沉积结晶涂层。该方法将产生致密且厚度均匀的结晶涂层。

8. 发明授权

US08270059B2 Multi-pane electrochromic windows 有权
标题翻译：多窗格电致变色窗
公开(公告)号：US08270059B2
公开(公告)日：2012-09-18
申请号：US12851514
申请日：2010-08-05
申请人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
发明人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
IPC分类号： G02F1/153
CPC分类号： G02F1/161 , C03C17/34 , C03C17/36 , C03C17/3657 , C03C17/3681 , E06B7/28 , E06B9/24 , E06B2009/2464 , E06B2009/2643 , G02F1/1347 , G02F1/1523 , G02F1/153 , G02F1/155 , G02F1/157 , G02F2001/1555 , H05B3/86 , H05B2203/013
摘要： Window units, for example insulating glass units (IGU's), that have at least two panes, each pane having an electrochromic device thereon, are described. Two optical state devices on each pane of a dual-pane window unit provide window units having four optical states. Window units described allow the end user a greater choice of how much light is transmitted through the electrochromic window. Also, by using two or more window panes, each with its own electrochromic device, registered in a window unit, visual defects in any of the individual devices are negated by virtue of the extremely small likelihood that any of the visual defects will align perfectly and thus be observable to the user.
摘要翻译：描述具有至少两个窗格的窗单元，例如中空玻璃单元（IGU），每个窗格上具有电致变色设备。双窗格窗单元的每个窗格上的两个光学状态设备提供具有四个光学状态的窗口单元。描述的窗口单元允许最终用户更多地选择通过电致变色窗口传输多少光。此外，通过使用两个或更多个窗玻璃，每个具有其自己的电致变色装置，登记在窗口单元中，任何单个装置中的视觉缺陷由于任何视觉缺陷将完美对准而非常小的可能性而被否定，并且因此可以观察到用户。

9. 发明申请

US20130021659A1 MULTI-PANE ELECTROCHROMIC WINDOWS 有权
公开(公告)号：US20130021659A1
公开(公告)日：2013-01-24
申请号：US13619772
申请日：2012-09-14
申请人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
发明人： Robin Friedman , Sridhar Kailasam , Rao Mulpuri , Ron Powell , Dhairya Shrivastava
IPC分类号： G02F1/161
CPC分类号： G02F1/161 , C03C17/34 , C03C17/36 , C03C17/3657 , C03C17/3681 , E06B7/28 , E06B9/24 , E06B2009/2464 , E06B2009/2643 , G02F1/1347 , G02F1/1523 , G02F1/153 , G02F1/155 , G02F1/157 , G02F2001/1555 , H05B3/86 , H05B2203/013
摘要： Window units, for example insulating glass units (IGU's), that have at least two panes, each pane having an electrochromic device thereon, are described. Two optical state devices on each pane of a dual-pane window unit provide window units having four optical states. Window units described allow the end user a greater choice of how much light is transmitted through the electrochromic window. Also, by using two or more window panes, each with its own electrochromic device, registered in a window unit, visual defects in any of the individual devices are negated by virtue of the extremely small likelihood that any of the visual defects will align perfectly and thus be observable to the user.

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