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1. 发明申请

US20050130118A1 Methods for identifying inhibitors of chlorophyll synthase 审中-公开
标题翻译：鉴定叶绿素合酶抑制剂的方法
公开(公告)号：US20050130118A1
公开(公告)日：2005-06-16
申请号：US10737014
申请日：2003-12-16
申请人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
发明人： Abubakr Aslamkhan , Lining Guo , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Jocelyn Kearney , Cory Christensen , Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes
IPC分类号： C12Q1/00 , G01N33/573
CPC分类号： G01N33/573
摘要： The present inventors have discovered that antisense suppression of a chlorophyll synthase (CS) gene results in plants exhibiting one or more of chlorosis, reduced growth, and altered development. Thus, the present inventors have discovered that the protein encoded by the CS gene is essential for normal plant growth and development, and is useful as a target for the identification of compounds as antibiotics and herbicides, especially herbicides. The present invention is directed to methods for identifying inhibitors of a CS enzyme by incubating a CS polypeptide with a chlorophyllide and a phopholipid substrate in the presence and absence of a test compound under conditions suitable for the CS enzyme activity, adding a solution to the incubation reactions comprising a water immiscible organic solvent, a water-soluble alcohol, and a water-soluble dye that absorbs in the range of one or both the excitation and emission wavelength ranges of the chlorophyllide substrate, and measuring the fluorescence of the incubation reactions at from about 650 to 750 nm, using from about 425 to 445 nm as excitation wavelength, wherein a decrease in the fluorescence in the presence of the test compound indicates that the compound is a CS inhibitor.
摘要翻译：本发明人已经发现，叶绿素合酶（CS）基因的反义抑制导致植物表现出一种或多种褪绿，生长减少和改变发育。因此，本发明人已经发现，由CS基因编码的蛋白质对于正常的植物生长和发育是必需的，并且可用作鉴定作为抗生素和除草剂，特别是除草剂的化合物的靶标。本发明涉及通过在适合于CS酶活性的条件下，在存在和不存在测试化合物的情况下，将CS多肽与叶绿素和磷脂底物一起温育来鉴定CS酶抑制剂的方法，向培养基中加入溶液包括与水不溶混的有机溶剂，水溶性醇和在所述叶绿素底物的激发和发射波长范围中的一个或两个的范围内吸收的水溶性染料的反应，以及测量从约650至750nm，使用约425至445nm作为激发波长，其中在测试化合物存在下荧光的降低表明该化合物是CS抑制剂。

2. 发明授权

US06770452B1 Methods for the identification of inhibitors of serine acetyltransferase activity in plants 失效
标题翻译：鉴定植物中丝氨酸乙酰转移酶活性抑制剂的方法
公开(公告)号：US06770452B1
公开(公告)日：2004-08-03
申请号：US10402818
申请日：2003-03-28
申请人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
发明人： John W Rice , Lining Guo , Keith Davis , Adel Zayed , Robert Ascenzi , Joseph Cameron Mitchell , Daniel N Riggsbee , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Susanne Kjemtrup , Carol Hamilton , Jeffrey Woessner , Jorn Gorlach
IPC分类号： C12Q148
CPC分类号： C12N9/1029 , C12N15/8261 , C12N15/8274 , C12Q1/48 , G01N2333/91057 , G01N2500/02 , Y02A40/146
摘要： The present inventors have discovered that serine acetyltransferase (SAT) is essential for plant growth. Specifically, the inhibition of SAT gene expression in plant seedlings results in reduced growth and altered pigmentation. Thus, SAT is useful as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of herbicides by measuring the activity of an SAT in the presence and absence of a compound, wherein an alteration of SAT activity in the presence of the compound indicates the compound as a candidate for a herbicide.
摘要翻译：本发明人发现丝氨酸乙酰转移酶（SAT）对于植物生长是必需的。具体地说，在植物幼苗中抑制SAT基因表达导致生长减少和色素沉着改变。因此，SAT可用作鉴定除草剂的靶标。因此，本发明提供了通过在化合物存在和不存在下测量SAT的活性来鉴定除草剂的方法，其中在化合物存在下SAT活性的改变表示作为除草剂候选物的化合物。

3. 发明申请

US20050112715A1 Methods for the identification of inhibitors of pectin esterase expression or activity in plants 审中-公开
标题翻译：用于鉴定植物中果胶酯酶表达或活性抑制剂的方法
公开(公告)号：US20050112715A1
公开(公告)日：2005-05-26
申请号：US10717879
申请日：2003-11-20
申请人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
发明人： Adel Zayed , Robert Ascenzi , Douglas Boyes , Rao Mulpuri , Neil Hoffman , Keith Davis , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
IPC分类号： C12Q1/44 , A01N25/00
CPC分类号： C12Q1/44 , G01N2500/00
摘要： The present inventors have discovered that pectin esterase is essential for plant growth. Specifically, the inhibition of pectin esterase gene expression resulted in seedlings that were damaged, short, and stunted at a very early growth stage. Thus, pectin esterase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit pectin esterase expression or activity, comprising: contacting a compound with a pectin esterase and detecting the presence and/or absence of binding between said compound and said pectin esterase, or detecting a decrease in pectin esterase expression or activity. The methods of the invention are useful for the identification of herbicides.
摘要翻译：本发明人发现，果胶酯酶对植物生长至关重要。具体来说，果胶酯酶基因表达的抑制导致幼苗在非常早期的生长阶段受损，短缺和发育迟缓。因此，果胶酯酶可以用作鉴定除草剂的靶标。因此，本发明提供了鉴定抑制果胶酯酶表达或活性的化合物的方法，包括：使化合物与果胶酯酶接触并检测所述化合物与所述果胶酯酶之间的结合的存在和/或不存在，或检测果胶酯酶表达或活性降低。本发明的方法可用于鉴定除草剂。

4. 发明申请

US20050100879A1 Methods for the identification of inhibitors of flavanone 3-hydroxylase expression or activity in plants 审中-公开
标题翻译：鉴定黄烷酮3-羟化酶抑制剂在植物中的表达或活性的方法
公开(公告)号：US20050100879A1
公开(公告)日：2005-05-12
申请号：US10702306
申请日：2003-11-06
申请人： Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes , Neil Hoffman , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
发明人： Keith Davis , Adel Zayed , Robert Ascenzi , Douglas Boyes , Neil Hoffman , Jorn Gorlach , Jeffrey Woessner , Carol Hamilton , Kenneth Phillips
IPC分类号： A01N25/00 , C12Q1/00 , C12Q1/26 , C12Q1/68 , G01N33/50
CPC分类号： G01N33/5097 , C12Q1/26 , G01N2430/20 , G01N2500/00
摘要： The present inventors have discovered that Flavanone 3-hydroxylase (“FHT”) is essential for plant growth. Specifically, the inhibition of FHT gene expression in plant seedlings results in small and chlorotic seedlings. Thus, FHT can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit FHT expression or activity. The present invention can be used to identify compounds having herbicide activity by contacting a compound with FHT and detecting the presence and/or absence of binding between the compound and the FHT, or alternatively, detecting a decrease in FHT expression or activity.
摘要翻译：本发明人已经发现，黄酮酮3-羟化酶（“FHT”）对于植物生长是必需的。具体来说，植物幼苗中FHT基因表达的抑制作用会导致幼苗和幼苗的生长。因此，FHT可用作鉴定除草剂的靶标。因此，本发明提供了鉴定抑制FHT表达或活性的化合物的方法。本发明可用于通过使化合物与FHT接触并检测化合物与FHT之间的结合的存在和/或不存在，或者检测FHT表达或活性的降低来鉴定具有除草剂活性的化合物。

5. 发明申请

US20050101485A1 Methods for the identification of inhibitors of biotin synthase expression or activity in plants 审中-公开
标题翻译：用于鉴定植物中生物素合酶表达抑制剂或活性的方法
公开(公告)号：US20050101485A1
公开(公告)日：2005-05-12
申请号：US10702364
申请日：2003-11-06
申请人： Neil Hoffman , Jorn Gorlach , Douglas Boyes , Adel Zayed , Jeffrey Woessner , Carol Hamilton , Keith Davis , Robert Ascenzi
发明人： Neil Hoffman , Jorn Gorlach , Douglas Boyes , Adel Zayed , Jeffrey Woessner , Carol Hamilton , Keith Davis , Robert Ascenzi
IPC分类号： A01N61/00 , C12Q1/48 , G01N33/50 , G01N33/573 , A01N25/00 , C12Q1/37
CPC分类号： G01N33/5097 , A01N61/00 , C12Q1/48 , G01N33/573 , G01N2500/02 , G01N2500/04
摘要： The present inventors have discovered that Biotin synthase (BS) is essential for plant growth. Specifically, inhibition of BS gene expression in plant seedlings results in severe chlorosis and reduced growth. Thus, BS can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit BS expression or activity, and as such, the methods of the invention are useful for the identification of herbicides.
摘要翻译：本发明人已经发现，生物素合酶（BS）对于植物生长是必需的。具体来说，植物幼苗中BS基因表达的抑制导致严重的萎黄症和生长减少。因此，BS可以用作鉴定除草剂的目标。因此，本发明提供了鉴定抑制BS表达或活性的化合物的方法，因此，本发明的方法可用于鉴定除草剂。

6. 发明授权

US08563806B2 Nematode inducible promotors and methods of use 失效
标题翻译：线虫诱导型启动子和使用方法
公开(公告)号：US08563806B2
公开(公告)日：2013-10-22
申请号：US12522260
申请日：2008-02-04
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/82 , C12N15/87 , C12N15/63
CPC分类号： C07K14/415 , C12N15/8227 , C12N15/8239
摘要： The invention provides isolated promoter polynucleotides that are root-specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.
摘要翻译：本发明提供了由植物寄生线虫根特异性和/或诱导的分离的启动子多核苷酸。本发明的启动子可用于控制植物根中感兴趣的核酸的表达，并且特别可用于控制编码破坏植物中寄生线虫饲养位点的形成或维持的试剂的转录。

7. 发明申请

US20100095403A1 PearlI1-Like Pathogen Control Genes and Methods of use in Plants 审中-公开
标题翻译： PearlI1样病原体控制基因及其在植物中的应用
公开(公告)号：US20100095403A1
公开(公告)日：2010-04-15
申请号：US12525847
申请日：2008-02-07
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Markus Frank , Anton Callaway
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Markus Frank , Anton Callaway
IPC分类号： A01H5/00 , C12N15/82 , C07H21/02
CPC分类号： C12N15/8285 , C07K14/415 , Y02A40/164
摘要： The invention provides pEARLI1-like polynucleotides which are capable of conferring increased nematode resistance to a plant. Also provided are inhibitory polynucleotides based on pEARLI1-like genes, which are capable of conferring resistance to necrotrophic fungi to plants. Specifically, the invention relates to transgenic plants, transgenic seeds, and expression vectors based on polynucleotides encoding pEARLI1-like genes, and methods of use thereof.
摘要翻译：本发明提供能够赋予对植物线虫抗性增加的pEARL1样多核苷酸。还提供了基于pEARL11-样基因的抑制性多核苷酸，其能够赋予对植物的坏死性真菌的抗性。具体地，本发明涉及基于编码pEARL11-样基因的多核苷酸的转基因植物，转基因种子和表达载体及其使用方法。

8. 发明申请

US20070250946A1 Monocot Ahass Sequences and Methods of Use 审中-公开
标题翻译： Monocot Ahass序列和使用方法
公开(公告)号：US20070250946A1
公开(公告)日：2007-10-25
申请号：US11659129
申请日：2005-08-04
申请人： Robert Ascenzi , Gregory Budziszewski , Genichi Kakefuda , Bijay Singh
发明人： Robert Ascenzi , Gregory Budziszewski , Genichi Kakefuda , Bijay Singh
IPC分类号： A01H1/00 , A01H5/00 , A01N43/50 , C07H21/04 , C07K1/00 , C12N15/00 , C12N5/00
CPC分类号： C12Y202/01006 , C12N9/88 , C12N15/8274 , C12N15/8278
摘要： Isolated polynucleotides that encode acetohydroxyacid synthase small subunit (AHASS) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and expression vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, expression cassettes, and expression vectors, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides are also described.
摘要翻译：描述了编码乙酰羟酸合酶小亚基（AHASS）多肽的分离的多核苷酸，以及编码这些多肽的氨基酸序列。描述了包含本发明的多核苷酸的表达盒和表达载体，以及用多核苷酸，表达盒和表达载体转化的植物和宿主细胞。还描述了使用多核苷酸增强植物对除草剂的抗性的方法。

9. 发明授权

US08691962B2 Peroxidase gene nematode inducible promotors and methods of use 失效
标题翻译：过氧化物酶基因线虫诱导型启动子及其使用方法
公开(公告)号：US08691962B2
公开(公告)日：2014-04-08
申请号：US12519530
申请日：2007-12-20
申请人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
发明人： Aaron Wiig , Robert Ascenzi , Xiang Huang , Sumita Chaudhuri , Rui-Guang Zhen , Yu Han
IPC分类号： C12N15/63 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8285 , C12N15/8239 , Y02A40/164
摘要： The invention provides isolated plant gene promoters and regulatory elements that are root specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.
摘要翻译：本发明提供了植物寄生线虫根系特异性和/或诱导的分离的植物基因启动子和调控元件。本发明的启动子可用于控制植物根中感兴趣的核酸的表达，并且特别可用于控制编码破坏植物中寄生线虫饲养位点的形成或维持的试剂的转录。

10. 发明申请

US20130289264A1 Methods And Compositions For Analyzing Ahasl Genes 有权
标题翻译：用于分析Ahasl基因的方法和组合
公开(公告)号：US20130289264A1
公开(公告)日：2013-10-31
申请号：US13527103
申请日：2012-06-19
申请人： Chengyan ZHAO , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan ZHAO , Robert Ascenzi , Bijay K. Singh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译：本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。硬粒小麦

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