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1. 发明申请

US20080107619A1 Carbohydrate based cellulase inhibitors as feeding stimulants in termites 审中-公开
标题翻译：碳水化合物基纤维素酶抑制剂作为白蚁中的喂食兴奋剂
公开(公告)号：US20080107619A1
公开(公告)日：2008-05-08
申请号：US11975314
申请日：2007-10-18
申请人： Michael E. Scharf , Xuguo Zhou , Faith M. Oi , Marsha M. Wheeler
发明人： Michael E. Scharf , Xuguo Zhou , Faith M. Oi , Marsha M. Wheeler
IPC分类号： A01N43/16 , A01N33/18 , A01N43/50
CPC分类号： A01N25/006
摘要： A method, composition and system for controlling termites wherein single carbohydrate-based compounds are used as both cellulase inhibitors and feeding stimulants. Di-saccharides, cellobioimidazole (CBI), fluoro-methyl cellobiose (FMCB), and mono-saccharides, fluoro-methyl glucose (FMG) and analogs thereof inhibit termite cellulose digestion, which leads to starvation or stimulates termite feeding to cause mortality. CBI, FMCB and FMG were tested against enzyme fractions that represented endogenous (foregut/salivary gland/midgut) and symbiotic (hindgut) termite cellulases in vitro and in vivo. Feeding stimulation by di-saccharides results in greater cellulase inhibitor intake throughout midrange concentrations (1 mM-10 mM), which is associated with significant termite mortality. In contrast, the monosaccharide inhibitor, FMG did not stimulate feeding, but did inhibit feeding at concentrations above 1 mM, causing mortality. With modification to create longer β-glycosidic chain lengths, the cellulase inhibitors identified herein can also be targeted to endoglucanase activity for increased efficacy and use as novel termite control compositions.
摘要翻译：用于控制白蚁的方法，组合物和系统，其中单一碳水化合物基化合物用作纤维素酶抑制剂和喂养兴奋剂。二糖，纤维二糖咪唑（CBI），氟甲基纤维二糖（FMCB）和单糖，氟甲基葡萄糖（FMG）及其类似物抑制白蚁纤维素消化，导致饥饿或刺激白蚁进食导致死亡。针对在体外和体内代表内源性（前肠/唾液腺/中肠）和共生（后肠）白蚁纤维素酶的酶分数测试CBI，FMCB和FMG。通过二糖的喂养刺激导致在中等浓度（1mM-10mM）下的更大的纤维素酶抑制剂摄入量，其与显着的白蚁死亡率相关。相比之下，单糖抑制剂FMG没有刺激喂养，但是在高于1mM的浓度下抑制进食，导致死亡。通过修改以产生更长的β-糖苷链长度，本文鉴定的纤维素酶抑制剂也可以靶向内切葡聚糖酶活性，以提高作为新白蚁控制组合物的功效和用途。

2. 发明授权

US08445240B2 Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products 有权
标题翻译：白蚁酶及其用于体外转化木质素材料至可发酵产品的用途
公开(公告)号：US08445240B2
公开(公告)日：2013-05-21
申请号：US13263107
申请日：2010-03-31
申请人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
发明人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
IPC分类号： C12P19/02 , C12N9/00 , C12P7/06 , C07H21/02
CPC分类号： C12N9/16 , C12P7/10 , C12P19/14 , C12P19/20 , Y02E50/16 , Y02E50/17
摘要： The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may be substantially purified, or used in a substantially unpurified form, to be admixed with a lignocellulose source to be converted to a fermentable product such as a sugar or a mixture of sugars. One aspect of the present disclosure, therefore, encompasses methods of converting a lignified plant material to a fermentable product, the method comprising obtaining a series of isolated polypeptides of a termite, wherein the series of polypeptides cooperate to convert a plant lignocellulose to a fermentable product; and incubating the series of polypeptides with a source of lignified plant material, under conditions allowing the polypeptides to cooperatively produce a fermentable product from the lignified plant material.
摘要翻译：本公开提供衍生自白蚁R flavipes的肠道的分离的核酸分子，包含可操作地插入其中的载体和分离的异源核酸分子的重组核酸分子，由此当转化到合适的宿主细胞系统中时，异源核酸序列表达为具有类似于在白蚁R. flavipes的肠中表达时的活性的多肽。重组核酸分子可以包含多于一个异源核酸分子，使得多于一个多肽可以由宿主系统表达。表达的多肽可以基本上纯化，或以基本上未纯化的形式使用，以与木质纤维素源混合以转化为可发酵产物，例如糖或糖的混合物。因此，本公开的一个方面包括将木质化植物材料转化为可发酵产品的方法，所述方法包括获得白蚁的一系列分离的多肽，其中所述一系列多肽协作以将植物木质纤维素转化为可发酵产物 ; 以及在允许多肽从木质化植物材料协同地产生可发酵产物的条件下，将一系列多肽与木质化植物材料来源一起孵育。

3. 发明授权

US07968525B1 Use of RNA interference to validate new termiticide target sites and a method of termite control 失效
标题翻译：使用RNA干扰来验证新的杀白蚁靶点和一种白蚁控制方法
公开(公告)号：US07968525B1
公开(公告)日：2011-06-28
申请号：US12324312
申请日：2008-11-26
申请人： Michael E. Scharf , Xuguo Zhou , Faith M. Oi , Marsha M. Wheeler , Matthew R. Tarver , Monique R. Coy
发明人： Michael E. Scharf , Xuguo Zhou , Faith M. Oi , Marsha M. Wheeler , Matthew R. Tarver , Monique R. Coy
IPC分类号： A61K31/70 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C12N15/1136 , C12N15/113 , C12N2310/14
摘要： Methods, matrix compositions and kits for increasing the mortality of termites (R. flavipes) and interfering with termite development using RNA interference techniques to target cellulase, lignocellulase, hexamerin, broad, farnesoic acid methyl transferase, cytochrome P450 and vitellogenin activity are provided.
摘要翻译：提供了用于增加白蚁（R. flavipes）死亡率并干扰使用RNA干扰技术靶向纤维素酶，木质纤维素酶，六聚体，广义，法呢酸甲基转移酶，细胞色素P450和卵黄蛋白酶活性的基质组合物和试剂盒。

4. 发明申请

US20120064580A1 TERMITE ENZYMES AND USES THEREOF FOR IN VITRO CONVERSION OF LIGNIN-CONTAINING MATERIALS TO FERMENTABLE PRODUCTS 有权
标题翻译：用于将含有含木质素的材料转化为可食用产品的温和酶及其用途
公开(公告)号：US20120064580A1
公开(公告)日：2012-03-15
申请号：US13263107
申请日：2010-03-31
申请人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
发明人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
IPC分类号： C12P19/00 , C12N5/10 , C12M1/00 , C12P19/02
CPC分类号： C12N9/16 , C12P7/10 , C12P19/14 , C12P19/20 , Y02E50/16 , Y02E50/17
摘要： The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may be substantially purified, or used in a substantially unpurified form, to be admixed with a lignocellulose source to be converted to a fermentable product such as a sugar or a mixture of sugars. One aspect of the present disclosure, therefore, encompasses methods of converting a lignified plant material to a fermentable product, the method comprising obtaining a series of isolated polypeptides of a termite, wherein the series of polypeptides cooperate to convert a plant lignocellulose to a fermentable product; and incubating the series of polypeptides with a source of lignified plant material, under conditions allowing the polypeptides to cooperatively produce a fermentable product from the lignified plant material.
摘要翻译：本公开提供衍生自白蚁R flavipes的肠道的分离的核酸分子，包含可操作地插入其中的载体和分离的异源核酸分子的重组核酸分子，由此当转化到合适的宿主细胞系统中时，异源核酸序列表达为具有类似于在白蚁R. flavipes的肠中表达时的活性的多肽。重组核酸分子可以包含多于一个异源核酸分子，使得多于一个多肽可以由宿主系统表达。表达的多肽可以基本上纯化，或以基本上未纯化的形式使用，以与木质纤维素源混合以转化为可发酵产物，例如糖或糖的混合物。因此，本公开的一个方面包括将木质化植物材料转化为可发酵产品的方法，所述方法包括获得白蚁的一系列分离的多肽，其中所述一系列多肽协作以将植物木质纤维素转化为可发酵产物 ; 以及在允许多肽从木质化植物材料协同地产生可发酵产物的条件下，将一系列多肽与木质化植物材料来源一起孵育。

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