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1. 发明授权

US08445240B2 Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products 有权
标题翻译：白蚁酶及其用于体外转化木质素材料至可发酵产品的用途
公开(公告)号：US08445240B2
公开(公告)日：2013-05-21
申请号：US13263107
申请日：2010-03-31
申请人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
发明人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
IPC分类号： C12P19/02 , C12N9/00 , C12P7/06 , C07H21/02
CPC分类号： C12N9/16 , C12P7/10 , C12P19/14 , C12P19/20 , Y02E50/16 , Y02E50/17
摘要： The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may be substantially purified, or used in a substantially unpurified form, to be admixed with a lignocellulose source to be converted to a fermentable product such as a sugar or a mixture of sugars. One aspect of the present disclosure, therefore, encompasses methods of converting a lignified plant material to a fermentable product, the method comprising obtaining a series of isolated polypeptides of a termite, wherein the series of polypeptides cooperate to convert a plant lignocellulose to a fermentable product; and incubating the series of polypeptides with a source of lignified plant material, under conditions allowing the polypeptides to cooperatively produce a fermentable product from the lignified plant material.
摘要翻译：本公开提供衍生自白蚁R flavipes的肠道的分离的核酸分子，包含可操作地插入其中的载体和分离的异源核酸分子的重组核酸分子，由此当转化到合适的宿主细胞系统中时，异源核酸序列表达为具有类似于在白蚁R. flavipes的肠中表达时的活性的多肽。重组核酸分子可以包含多于一个异源核酸分子，使得多于一个多肽可以由宿主系统表达。表达的多肽可以基本上纯化，或以基本上未纯化的形式使用，以与木质纤维素源混合以转化为可发酵产物，例如糖或糖的混合物。因此，本公开的一个方面包括将木质化植物材料转化为可发酵产品的方法，所述方法包括获得白蚁的一系列分离的多肽，其中所述一系列多肽协作以将植物木质纤维素转化为可发酵产物 ; 以及在允许多肽从木质化植物材料协同地产生可发酵产物的条件下，将一系列多肽与木质化植物材料来源一起孵育。

2. 发明申请

US20120064580A1 TERMITE ENZYMES AND USES THEREOF FOR IN VITRO CONVERSION OF LIGNIN-CONTAINING MATERIALS TO FERMENTABLE PRODUCTS 有权
标题翻译：用于将含有含木质素的材料转化为可食用产品的温和酶及其用途
公开(公告)号：US20120064580A1
公开(公告)日：2012-03-15
申请号：US13263107
申请日：2010-03-31
申请人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
发明人： Michael E. Scharf , Drion G. Boucias , Aurelien Tartar , Monique R. Coy , Xuguo Zhou , Tamer Ibrahim Zaki Salem , Sanjay B. Jadhao , Marsha M. Wheeler
IPC分类号： C12P19/00 , C12N5/10 , C12M1/00 , C12P19/02
CPC分类号： C12N9/16 , C12P7/10 , C12P19/14 , C12P19/20 , Y02E50/16 , Y02E50/17
摘要： The disclosure provides isolated nucleic acid molecules derived from the gut of the termite R flavipes, recombinant nucleic acid molecules comprising a vector and an isolated heterologous nucleic acid molecule operably inserted therein, whereby, when transformed into an appropriate host cell system, the heterologous nucleic acid sequence is expressed as a polypeptide having an activity similar to that when expressed in the gut of the termite R. flavipes. The recombinant nucleic acid molecules can comprise more than one heterologous nucleic acid molecule such that more than one polypeptide may be expressed by the host system. The expressed polypeptides may be substantially purified, or used in a substantially unpurified form, to be admixed with a lignocellulose source to be converted to a fermentable product such as a sugar or a mixture of sugars. One aspect of the present disclosure, therefore, encompasses methods of converting a lignified plant material to a fermentable product, the method comprising obtaining a series of isolated polypeptides of a termite, wherein the series of polypeptides cooperate to convert a plant lignocellulose to a fermentable product; and incubating the series of polypeptides with a source of lignified plant material, under conditions allowing the polypeptides to cooperatively produce a fermentable product from the lignified plant material.
摘要翻译：本公开提供衍生自白蚁R flavipes的肠道的分离的核酸分子，包含可操作地插入其中的载体和分离的异源核酸分子的重组核酸分子，由此当转化到合适的宿主细胞系统中时，异源核酸序列表达为具有类似于在白蚁R. flavipes的肠中表达时的活性的多肽。重组核酸分子可以包含多于一个异源核酸分子，使得多于一个多肽可以由宿主系统表达。表达的多肽可以基本上纯化，或以基本上未纯化的形式使用，以与木质纤维素源混合以转化为可发酵产物，例如糖或糖的混合物。因此，本公开的一个方面包括将木质化植物材料转化为可发酵产品的方法，所述方法包括获得白蚁的一系列分离的多肽，其中所述一系列多肽协作以将植物木质纤维素转化为可发酵产物 ; 以及在允许多肽从木质化植物材料协同地产生可发酵产物的条件下，将一系列多肽与木质化植物材料来源一起孵育。

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