专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120141997A1 Compositions And Methods For Free-Solution Conjugate Nucleic Acid Analysis 审中-公开
标题翻译：自由溶液共轭核酸分析的组成和方法
公开(公告)号：US20120141997A1
公开(公告)日：2012-06-07
申请号：US13372576
申请日：2012-02-14
申请人： Robert J. Meagher , Wyatt N. Vreeland , Annelise E. Barron
发明人： Robert J. Meagher , Wyatt N. Vreeland , Annelise E. Barron
IPC分类号： C12Q1/68 , G01N33/559
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2565/627 , C12Q2565/125 , C12Q2533/101
摘要： The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.
摘要翻译：本发明提供了用于进行核酸分子的自由结合分析的组合物和方法。例如，本发明提供用于基因分型的多重单碱基延伸测定。特别地，本发明提供了一系列用于实现核酸反应产物的高分辨率分离的分散聚酰胺“拖曳标签”。

2. 发明授权

US08114599B2 Compositions and methods for free-solution conjugate nucleic acid analysis 有权
标题翻译：自由溶液共轭核酸分析的组成和方法
公开(公告)号：US08114599B2
公开(公告)日：2012-02-14
申请号：US11960185
申请日：2007-12-19
申请人： Robert J. Meagher , Wyatt N Vreeland , Annelise E. Barron
发明人： Robert J. Meagher , Wyatt N Vreeland , Annelise E. Barron
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2565/627 , C12Q2565/125 , C12Q2533/101
摘要： The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.
摘要翻译：本发明提供了用于进行核酸分子的自由结合分析的组合物和方法。例如，本发明提供用于基因分型的多重单碱基延伸测定。特别地，本发明提供了一系列用于实现核酸反应产物的高分辨率分离的分散聚酰胺“拖曳标签”。

3. 发明授权

US09198645B2 Controlled vesicle self-assembly in continuous two phase flow microfluidic channels 有权
标题翻译：在连续两相流微流体通道中控制囊泡自组装
公开(公告)号：US09198645B2
公开(公告)日：2015-12-01
申请号：US10895366
申请日：2004-07-21
申请人： Andreas Jahn , Wyatt N. Vreeland , Laurie E. Locascio , Michael Gaitan
发明人： Andreas Jahn , Wyatt N. Vreeland , Laurie E. Locascio , Michael Gaitan
IPC分类号： A61K9/127 , A61B17/00
CPC分类号： A61B17/0057 , A61B2017/00654 , A61B2017/00659 , A61B2017/00898 , A61K9/1277
摘要： Methods for the formation of liposomes that encapsulate reagents in a continuous 2-phase flow microfluidic network with precision control of size, for example, from 100 nm to 300 nm, by manipulation of liquid flow rates are described. By creating a solvent-aqueous interfacial region in a microfluidic format that is homogenous and controllable on the length scale of a liposome, fine control of liposome size and polydispersity can be achieved.
摘要翻译：描述了通过操纵液体流速来形成将试剂封装在连续的2相流微流体网络中的脂质体的方法，其精确控制尺寸例如为100nm至300nm。通过在脂质体的长度尺度上产生均匀且可控的微流体形式的溶剂 - 水界面区域，可以实现脂质体大小和多分散性的精细控制。

4. 发明授权

US07718046B2 Micellar gradient focusing 有权
标题翻译：胶束梯度聚焦
公开(公告)号：US07718046B2
公开(公告)日：2010-05-18
申请号：US10864485
申请日：2004-06-10
申请人： David J. Ross , Peter B. Howell , Wyatt N. Vreeland
发明人： David J. Ross , Peter B. Howell , Wyatt N. Vreeland
IPC分类号： B01D57/02
CPC分类号： C07K1/26 , G01N30/02 , G01N30/34 , B01D15/3842
摘要： A method and device are provided for affinity gradient focusing for directing at least one analyte in a solution containing a pseudostationary phase and located in a channel such as a capillary or a microchannel. The method includes establishing a steady-state spatial gradient in a retention factor of the pseudostationary phase for the at least one analyte. The analyte is caused to be moved within the channel whereby the concentration of the at least one analyte changes at one or more positions along the gradient. The pseudostationary phase is charged and the analyte is either neutral or charged or alternatively, the pseudostationary phase is neutral and the analyte is charged. The device may include a fluid channel, a pseudostationary phase having a retention factor gradient, an electrical current source and a pump system for establishing the bulk flow in the solution in the channel.
摘要翻译：提供了一种用于亲和梯度聚焦的方法和装置，用于将至少一种分析物引导到含有假阶段并且位于诸如毛细管或微通道的通道中的溶液中。该方法包括在至少一种分析物的假定相的保留因子中建立稳态空间梯度。导致分析物在通道内移动，由此至少一种分析物的浓度在沿梯度的一个或多个位置处变化。假定相被充电，分析物是中性的或带电的，或者替代地，假定相是中性的并且分析物被充电。该装置可以包括流体通道，具有保持因子梯度的假定相，电流源和用于在通道中的溶液中建立大量流的泵系统。

5. 发明申请

US20080227211A1 COMPOSITIONS AND METHODS FOR FREE-SOLUTION CONJUGATE NUCLEIC ACID ANALYSIS 有权
标题翻译：用于自由解决方案的组合物和方法结合核酸分析
公开(公告)号：US20080227211A1
公开(公告)日：2008-09-18
申请号：US11960185
申请日：2007-12-19
申请人： Robert J. Meagher , Wyatt N. Vreeland , Annelise E. Barron
发明人： Robert J. Meagher , Wyatt N. Vreeland , Annelise E. Barron
IPC分类号： G01N27/26
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2565/627 , C12Q2565/125 , C12Q2533/101
摘要： The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.
摘要翻译：本发明提供了用于进行核酸分子的自由结合分析的组合物和方法。例如，本发明提供用于基因分型的多重单碱基延伸测定。特别地，本发明提供了一系列用于实现核酸反应产物的高分辨率分离的分散聚酰胺“拖曳标签”。

6. 发明申请

US20130168885A1 DEVICE AND METHOD FOR FORMATION OF VESICLES 审中-公开
标题翻译：用于形成VESICLES的装置和方法
公开(公告)号：US20130168885A1
公开(公告)日：2013-07-04
申请号：US13733882
申请日：2013-01-03
申请人： DONNA M. OMIATEK , RENEE R. HOOD , FRANCISCO JAVIER ATENCIA-FERNANDEZ , DON L. DEVOE , WYATT N. VREELAND
发明人： DONNA M. OMIATEK , RENEE R. HOOD , FRANCISCO JAVIER ATENCIA-FERNANDEZ , DON L. DEVOE , WYATT N. VREELAND
IPC分类号： B01J13/00
CPC分类号： B01J13/00 , A61K9/1277 , B01J13/14
摘要： A device and method for the formation of vesicles is disclosed herein. The device comprises a fluid introduction zone and a vesicle formation zone. The fluid introduction zone comprises a first inlet and a second inlet configured and disposed to provide parallel flow of an outer flow stream, flowing from the first inlet, sheathing an inner flow stream, flowing from the second inlet. The vesicle formation zone is configured and disposed to receive the parallel flow of the outer flow stream sheathing the inner flow stream and configured for a controlled and substantially uniform dispersion of an organic material, flowing in the inner flow stream, at a plane perpendicular to the vesicle formation zone.
摘要翻译：本文公开了一种用于形成囊泡的装置和方法。该装置包括流体引入区和囊泡形成区。所述流体引入区包括第一入口和第二入口，所述第一入口和第二入口被构造和设置成提供从所述第一入口流出的外流动流的平行流，所述第二入口包含从所述第二入口流出的内流动流。泡囊形成区域被配置和设置成接收外层流动流的平行流，所述外流动流包围内部流动流并且被配置为在与内部流动流垂直的平面处流动在内部流动流中的有机材料的受控且基本上均匀的分散囊泡形成区。

7. 发明授权

US07494577B2 Tandem isotachophoresis/zone electrophoresis method and system 有权
标题翻译：串联等电泳/区域电泳方法和系统
公开(公告)号：US07494577B2
公开(公告)日：2009-02-24
申请号：US10676857
申请日：2003-09-30
申请人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
发明人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
IPC分类号： G01N27/447
CPC分类号： B01L3/5027 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/049 , C07K1/26 , C07K1/28 , G01N27/44773
摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected , in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charge sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separated by zone electrophoresis.
摘要翻译：公开了一种分离具有给定负电荷并包含在样品中的组分的方法。在一个实施方案中，该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质，其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积进入和通过样品体积区域的上游电极有效地超过电荷样品组分。通过在通道末端继续施加电位，通过区域电泳分离的堆叠样品体积中的带电样品组分。

8. 发明授权

US06685813B2 Tandem isotachophoresis/zone electrophoresis method and system 有权
标题翻译：串联等电泳/区域电泳方法和系统
公开(公告)号：US06685813B2
公开(公告)日：2004-02-03
申请号：US09933993
申请日：2001-08-20
申请人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
发明人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
IPC分类号： B01D5902
CPC分类号： B01L3/5027 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/049 , C07K1/26 , C07K1/28 , G01N27/44773
摘要： A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.
摘要翻译：公开了一种分离具有给定负电荷并包含在样品中的组分的方法。在一个实施方案中，该方法包括将具有样品的微通道加载到放置在具有选定浓度的可滴定物质的后缘电解质和前沿电解质之间。通过在微通道上施加电压电位，通过等速电泳施加样品叠层中的带电组分，并且通过在上游端电极处电解形成的电解羟基或氢离子迁移到后缘离子缓冲液中，滴定其中的可滴定物质，其中选择后缘电解质中的可滴定物质的浓度，相对于上游通道区域的长度和样品加载体积，以允许样品在电离离子迁移之前堆积成相对小的样品体积进入和通过样品体积区域的上游电极有效地超过带电的样品组分。通过在通道末端继续施加电位，通过区域电泳分离堆叠样品体积中的带电样品组分。

9. 发明授权

US06818113B2 Microfluidic device with sample injector and method of using 有权
标题翻译：具有样品注射器的微流体装置和使用方法
公开(公告)号：US06818113B2
公开(公告)日：2004-11-16
申请号：US09780638
申请日：2001-02-10
申请人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
发明人： Stephen J. Williams , Hong Dong Tan , Hung Pin Kao , Wyatt N. Vreeland
IPC分类号： B01D5702
CPC分类号： C07K1/28 , B01L3/5027 , B01L3/502776 , B01L3/502784 , B01L2200/0605 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/049 , G01N27/44743 , G01N27/44747 , G01N27/44773 , G01N27/44791
摘要： A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.
摘要翻译：公开了一种用于将液体样品注入微流体装置中的电解质通道的方法和装置。该装置具有通道网络，其包括具有上游和下游通道部分的电解质通道以及分别在第一，第二和第三端口处的两个通道部分之间与电解质通道相交的第一，第二和第三侧通道。在该方法中，将样品电动移动到电解质通道中，以在其中形成限定的样品体积。通过同时控制施加到三个侧通道的电压以及上游和下游通道端部中的至少一个，样品体积元件可以被成形为具有期望的前沿和后沿形状和/或样品组分的分布在音量元素内。

10. 发明授权

US07572357B2 Chiral temperature gradient focusing 失效
标题翻译：手性温度梯度聚焦
公开(公告)号：US07572357B2
公开(公告)日：2009-08-11
申请号：US11039955
申请日：2005-01-24
申请人： David J. Ross , Wyatt N. Vreeland , Karin M. Balss
发明人： David J. Ross , Wyatt N. Vreeland , Karin M. Balss
IPC分类号： G01N27/447
CPC分类号： G01N27/44795 , G01N27/44734
摘要： A method and device are provided for concentrating and separating materials in fluids within a fluidic device having a fluid conduit such as a channel or capillary. The concentration is achieved by balancing the electrophoretic velocity of a material against the bulk flow of fluid in the presence of a temperature gradient. An additive is added to the fluid which interacts with the material and which modifies the normal electrophoretic mobility of the material. Using an appropriate fluid, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity so that the electrophoretic and bulk velocities sum to zero at a unique position along the conduit and the material will be focused at that position. The method and device may be adapted for use with a variety of materials including fluorescent dyes, amino acids, proteins, DNA and to concentrate a dilute material.
摘要翻译：提供了一种方法和装置，用于在具有诸如通道或毛细管的流体导管的流体装置内的流体中浓缩和分离材料。通过在存在温度梯度的情况下平衡材料的电泳速度与流体的体积流动来实现浓度。将添加剂添加到与材料相互作用并且改变材料的正常电泳迁移率的流体中。使用适当的流体，温度梯度可以产生相应的电泳速度梯度，使得电泳和体积速度在沿着导管的独特位置处总和为零，并且材料将聚焦在该位置。该方法和装置可适用于各种材料，包括荧光染料，氨基酸，蛋白质，DNA和浓缩稀释材料。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式