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    • 1. 发明申请
    • Diffusion-controlling sensor layer
    • 扩散控制传感器层
    • US20070292912A1
    • 2007-12-20
    • US11893800
    • 2007-08-16
    • Wolfgang KreissGunther EberzClaus WeisemannKlaus Burger
    • Wolfgang KreissGunther EberzClaus WeisemannKlaus Burger
    • C12Q1/68
    • G01N33/5008C12Q1/18G01N21/76G01N33/558
    • The invention relates to a diffusion-controlling sensor layer for detecting the biological effect of substances consisting of a diffusion-controlling matrix and sensors suspended therein. The invention further comprises a method and an apparatus for the location-dependent detection of the biological effect of substances using the sensor layer according to the invention (“imaging” of the spatial distribution of biological activity). A further embodiment of the method according to the invention relates to the coupling of investigations of the biological activity of substances with chromatographic and electrophoretic separation techniques so that biologically active individual substances can be identified directly in mixtures. An additional embodiment of the method according to the invention comprises the direct combination of the detection of the effect via sensor layers with chromatographic and electrophoretic separation techniques which are in turn coupled to spectroscopic techniques. This close coupling of substance separation, detection of effect and spectroscopy directly provides results on the structure of the biologically active substances in a mixture of substances.
    • 本发明涉及一种扩散控制传感器层,用于检测由扩散控制基质和悬浮在其中的传感器组成的物质的生物学效应。 本发明还包括用于使用根据本发明的传感器层(“成像”生物活性的空间分布)的物质的位置相关检测物质的生物学效应的方法和装置。 根据本发明的方法的另一个实施方案涉及物质生物活性的研究与色谱和电泳分离技术的偶联,使得生物活性的个体物质可以直接在混合物中鉴定。 根据本发明的方法的另外的实施例包括通过传感器层检测效应与色谱和电泳分离技术的直接组合,这些技术又耦合到光谱技术。 物质分离的紧密耦合,效应和光谱的检测直接提供了物质混合物中生物活性物质结构的结果。